We narrowed to 796 results for: arc

1. Antibodies 101: Chimeric Antibodies

   Type

   Blog Post

   Published

   April 25, 2023, 1:15 p.m.

   ...Protein A and Protein B interact. You read extensive research on the two proteins and come up with a great experimental...

2. Don’t Wait To Launch Your Health Science Startup

   Type

   Blog Post

   Published

   Aug. 25, 2015, 2:30 p.m.

   ...successful startup management team). Sreyan is a research associate at Harvard Medical School and runs everything...

3. Even more elegant: Single injection CRISPR/Cas9 in C. elegans

   Type

   Blog Post

   Published

   July 7, 2015, 3:36 p.m.

   ...were released, signaling a new era in C. elegans research. Homology directed repair (HDR), which enables...

4. Rewiring Metabolic Circuitry with CRISPR RNA Scaffolds [Video]

   Type

   Blog Post

   Published

   April 7, 2015, 4:21 p.m.

   ...introduce heterologous genes, but the trend of research has shifted in recent years toward tinkering with...

5. Hot Plasmids: FRET-Based Biosensors

   Type

   Blog Post

   Published

   May 6, 2014, 1:07 p.m.

   ...cultures. The optimized Twitch sensors allowed the researchers to see tonic action potential firing in neurons...

6. Mentoring for Scientists: I Have a Mentor, What Now?

   Type

   Blog Post

   Published

   March 6, 2014, 7:12 p.m.

   ...podcast interview with Harvard Medical School researcher Connie Cepko to learn about her mentoring style...

7. The Importance of High Titer for AAV Transductions

   Type

   Blog Post

   Published

   March 1, 2018, 2:11 p.m.

   ...diluting to confirm. Luke Makana Hanley is a Research Team Lab Tech at Addgene working on methods to...

8. Learning Technical Skills at a Lab Central Workshop

   Type

   Blog Post

   Published

   Feb. 20, 2018, 2:30 p.m.

   ...Addgene strives to be a great resource for the research community, so it is extremely important for us...

9. Plasmids 101: NGS Quality Control for Pooled Libraries

   Type

   Blog Post

   Published

   Oct. 26, 2017, 1:59 p.m.

   ... libraries. Pooled libraries can be a powerful research tool, but their proper use requires careful amplification...

10. New and Upcoming Viral Vectors - September 2019

    Type

    Blog Post

    Published

    Sept. 12, 2019, 1 p.m.

    ... viral protocols and videos Find AAVs for your research ...

11. Visualizing Genomic Loci with CRISPR-Sirius

    Type

    Blog Post

    Published

    April 18, 2019, 12:53 p.m.

    ...plasmids for visualization Find plasmids for your research Read our CRISPR guide ...

12. Quantifying DNA? Here are Five DNA Quantification Methods to Consider

    Type

    Blog Post

    Published

    July 21, 2020, 1:15 p.m.

    ...Molecular Biology Reference Find plasmids for your research Browse protocols for molecular biology, cloning...

13. Virtual Science Conference Coming Up? Three Addgenies Share Their Experience

    Type

    Blog Post

    Published

    June 30, 2020, 1:15 p.m.

    ...interaction might not work when you are giving a research talk, there are still other ways to make your ...

14. Photosensitizer Induced Cell Ablation with FAP-TAP MG-2I-dL5**

    Type

    Blog Post

    Published

    Sept. 19, 2017, 1:20 p.m.

    ...cells. Are you ready to use MG-2I-dL5** with your research? Let us know about your experiences using photosensitizers...

15. Pushing the Limits of DNA Assembly

    Type

    Blog Post

    Published

    Sept. 6, 2017, 1:01 p.m.

    ... obtaining a plasmid with the gene of interest (search Addgene plasmids by gene here), designing primers...

16. Plasmids 101: Gram Negative and Positive Bacteria

    Type

    Blog Post

    Published

    Aug. 2, 2022, 1:15 p.m.

    ...Staining. https://serc.carleton.edu/microbelife/research_methods/microscopy/gramstain.html Smith, A. & Hussey...

17. Viral Vectors 101: Inverted Terminal Repeats

    Type

    Blog Post

    Published

    Oct. 19, 2023, 1:15 p.m.

    ...knowledge of ITRs is still limited, but this area of research is important for downstream AAV applications.  ...

18. Choosing Your Fluorescent Proteins for Multi-Color Imaging

    Type

    Blog Post

    Published

    Oct. 9, 2014, 3 p.m.

    ... was a fellow at the Bauer Center for Genomics Research at Harvard University.       ...

19. Plasmids 101: How to Verify Your Plasmid Using a Restriction Digest Analysis

    Type

    Blog Post

    Published

    Aug. 28, 2014, 3:34 p.m.

    ...well as the predicted size of the insert. You can search NCBI for YGOI to find the particular reference ...

20. Plasmids 101: Transformation, Transduction, Bacterial Conjugation, and Transfection

    Type

    Blog Post

    Published

    June 25, 2019, 12:54 p.m.

    ... technique in molecular biology and scientific research. There are multiple ways foreign DNA can be introduced...

21. 15 Years of Addgene: The Top 15 Plasmids

    Type

    Blog Post

    Published

    Jan. 8, 2019, 1:54 p.m.

    ...Browse our CRISPR collection Find plasmids for your research  Learn about our viral service ...

22. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

    Type

    Blog Post

    Published

    May 26, 2020, 1:15 p.m.

    ...our CRISPR guide Find CRISPR plasmids for your research Find resources and software for your CRISPR experiment...

23. Quick Guide to Working with Drosophila Part 3: Genome Engineering in Flies

    Type

    Blog Post

    Published

    July 28, 2017, 1:30 p.m.

    ...consumption. Drosophila are also used in cancer research (3). The sky is the limit. As mentioned earlier...

24. Improved Plasmid Maps Powered by SnapGene

    Type

    Blog Post

    Published

    June 29, 2017, 1:06 p.m.

    ...tabs. BLAST Use the Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine ...

25. Luminescent Imaging with Nano-lanterns

    Type

    Blog Post

    Published

    May 25, 2017, 2:30 p.m.

    ...by both autofluorescence and photobleaching. Researchers have long been interested in using luminescence...

26. Plasmids 101: Methylation and Restriction Enzymes

    Type

    Blog Post

    Published

    June 30, 2016, 2:30 p.m.

    ... Marcy Patrick...

27. How to Keep a Lab Notebook for Bioinformatic Analyses

    Type

    Blog Post

    Published

    June 2, 2016, 2:30 p.m.

    ...the simple rules that I try to follow in my own research. Again, some of this may not apply or may not ...

28. Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers

    Type

    Blog Post

    Published

    July 28, 2015, 2:30 p.m.

    ... the ENCODE Consortium in Cold Spring Harbor in March. A number of groups were excited and asked for the...

29. Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens

    Type

    Blog Post

    Published

    Feb. 24, 2015, 7:50 p.m.

    ...Brigham MD, Trevino AE, Joung J, Abudayyeh OO, Barcena C, Hsu PD, Habib N, Gootenberg JS, Nishimasu H,...

30. Plasmids 101: Yeast Vectors

    Type

    Blog Post

    Published

    Feb. 25, 2014, 7:11 p.m.

    ...  Marcy Patrick...

31. Form Your Own Peer Mentoring Group: A How-To Guide for Scientists

    Type

    Blog Post

    Published

    Feb. 18, 2014, 4:22 p.m.

    ...podcast interview with Harvard Medical School researcher Connie Cepko to learn about her mentoring style...

32. Seven Tips for Using LinkedIn as a Scientist

    Type

    Blog Post

    Published

    Oct. 1, 2019, 1:21 p.m.

    ... culture and connections. Start your interview research there! 6) Your LinkedIn profile should be as good...

33. Lighting Up Cell Signaling with Photoswitchable Kinases

    Type

    Blog Post

    Published

    July 25, 2017, 1:20 p.m.

    ...photoswitchable kinases in vivo with C. elegans. Researchers commonly use rectal bacterial infection to induce...

34. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

    Type

    Blog Post

    Published

    April 13, 2017, 2:30 p.m.

    ...Levine, B. (2010). Methods in Mammalian Autophagy Research. Cell,140(3), 313-326. doi:10.1016/j.cell.2010.01.028...

35. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

    Type

    Blog Post

    Published

    Dec. 8, 2016, 3:30 p.m.

    ...protocols! We invite feedback from all members of the research community to help us develop these resources further...

36. Antibodies 101: Selecting the Right Antibody

    Type

    Blog Post

    Published

    March 15, 2022, 1:15 p.m.

    ...experiments require antibodies. You run a quick internet search for the required antibodies and find….hundreds ...

37. Antibodies 101: Fab Fragments

    Type

    Blog Post

    Published

    Sept. 5, 2024, 1:15 p.m.

    ...alone. Full-sized antibodies dominate the world of research affinity reagents, and for good reason. However...

38. The time and cost required to make a plasmid

    Type

    Blog Post

    Published

    March 23, 2023, 1:15 p.m.

    ...special collection pages to more easily find plasmids Search for a plasmid in Addgene’s repository ...

39. To Each HIS Own

    Type

    Blog Post

    Published

    March 2, 2023, 2:15 p.m.

    ...Much of today's biological research requires a close examination of specific proteins within a system...

40. Giving gRNAs a Facelift - Synthetic and Beyond

    Type

    Blog Post

    Published

    Oct. 20, 2022, 1:15 p.m.

    ...stable than most macromolecules used in scientific research such as DNA or protein. It comes as no surprise...

41. Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number

    Type

    Blog Post

    Published

    Sept. 4, 2018, 12:53 p.m.

    ...Researchers express genes of interest from plasmids in order to study gene function or to engineer cells...

42. Split-BioID: An Improved Method for Studying Protein-Protein Interactions

    Type

    Blog Post

    Published

    May 1, 2018, 1:57 p.m.

    ...half-lives. Interested in using Split-BioID with your research? Plasmids are available from Addgene! References...

43. Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors

    Type

    Blog Post

    Published

    Aug. 20, 2019, 12:31 p.m.

    ... viral protocols and videos Find AAVs for your research ...

44. Fluorescent Proteins 101: Introduction to FRET

    Type

    Blog Post

    Published

    June 27, 2017, 1:03 p.m.

    ... Before constructing your own FRET probe, try searching PubMed for articles describing the FRET tool that...

45. Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

    Type

    Blog Post

    Published

    Dec. 14, 2021, 2:15 p.m.

    ...p300 or the DNA methyltransferase DNMT3A allows researchers to easily add biochemical marks to genetic regions...

46. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

    Type

    Blog Post

    Published

    Nov. 14, 2024, 2:15 p.m.

    ...Cas9 system in human cell lines. Nucleic Acids Research. 2014;43(3):e17. doi:10.1093/nar/gku1198...

47. Antibodies 101: Stripping and Reprobing Western Blots

    Type

    Blog Post

    Published

    Aug. 1, 2024, 1:15 p.m.

    ...reprobe your western blot several times — some researchers report up to ten cycles! Just remember that a...

48. Antibodies 101: Choosing the Right Isotype

    Type

    Blog Post

    Published

    April 6, 2023, 3 p.m.

    ...After all, there’s no sense in spending valuable research dollars on a new secondary antibody if you don...

49. Progress Towards a PAM-Free CRISPR

    Type

    Blog Post

    Published

    March 7, 2024, 2:39 p.m.

    ...alleles, and the growing need for gene editing in research, the old PAM constraints just won’t cut it anymore...

50. Hot Plasmids and Viral Preps - January 2021

    Type

    Blog Post

    Published

    Jan. 5, 2021, 2:15 p.m.

    ...fluorescence-activated cell sorting (FACS) helps researchers sift through thousands of cells to identify cells...