We narrowed to 331 results for: AGA
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TypeBlog PostPublishedApril 11, 2024, 1:15 p.m....RepB) necessary for replication in various hosts (Pagatto and Dillon 2001). The plasmid has its own replication...
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Antibodies 101: Reading a Flow Plot
TypeBlog PostPublishedFeb. 6, 2024, 3:13 p.m....different populations using conjugated antibodies against CD11c and CD123. You can see that mDCs are CD11c... -
CRISPR-based Genome Editing Systems For Editing in E. coli
TypeBlog PostPublishedApril 13, 2021, 1:15 p.m....Increasing rounds of transfer on transposition-inducing agar lead to higher cargo copy numbers. (D) The effect... -
The PAM Requirement and Expanding CRISPR Beyond SpCas9
TypeBlog PostPublishedAug. 20, 2020, 2:30 p.m....NNNNRYAC Streptococcus thermophilus (St) NNAGAAW Treponema denticola (Td) NAAAAC ~20 additional... -
Four Base Editing Reporters to Monitor and Enrich Editing in Real-time
TypeBlog PostPublishedJuly 7, 2020, 1:15 p.m....D, Serebrenik A, Shaban N, Brown WL, Donati F, Munagala U, Conticello SG, Harris RS (2018) A fluorescent... -
Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone
TypeBlog PostPublishedFeb. 18, 2020, 2:15 p.m....Iwasa, Shawnna Buttery, Kat Milligan-Myhre, and Pragati Chengappa. Financial and moral supporters Denise... -
Deciphering the Mysteries of Behavior with Viral Vectors
TypeBlog PostPublishedSept. 17, 2019, 12:40 p.m....open new ways for a novel therapeutic approach against drug addiction. Anxiety tests Rodents have a natural... -
Sonic Hedgehog? Sleeping Beauty? Learn About the Genes Behind Addgene’s Conference Room Names
TypeBlog PostPublishedApril 2, 2019, 1:44 p.m....squash" originated from the squash itself, but think again. Spaghetti squash is actually a component of the... -
Designing Your Chalk Talk for the Academic Job Interview
TypeBlog PostPublishedMarch 12, 2019, 1:37 p.m....favor a different model. Finding evidence for or against a model is a standard scientific strategy. However... -
Overcoming the AAV Size Limitation for CRISPR Delivery
TypeBlog PostPublishedSept. 16, 2020, 1 p.m.... Barrangou R, Boyaval P, Fremaux C, Horvath P, Magadán AH, Moineau S (2010) The CRISPR/Cas bacterial immune... -
AAV Titers: Where Do They Come From And What Do They Mean?
TypeBlog PostPublishedNov. 15, 2017, 3 p.m....reliable, or if the sample needs to be quantified again. Some of the issues with titrating by qPCR are ... -
Bioinformatics at Addgene
TypeBlog PostPublishedJuly 14, 2022, 1:15 p.m....QC'ing data at Addgene is like standing under a Niagara Falls sized rainbow Over the years we have... -
Changing Labor Laws Bring Increased Postdoc Wages
TypeBlog PostPublishedOct. 25, 2016, 2:30 p.m....as of October 1st the salary minimum was raised again to $51,600, itself a raise of 3.2%. Other institutions... -
Plasmids 101: E. coli Strains for Protein Expression
TypeBlog PostPublishedFeb. 10, 2015, 3:06 p.m....of several popular strains of E. coli for DNA propagation. While great for cloning purposes, these E. coli... -
Antibodies 101: Normalization and Loading Controls for Western Blots
TypeBlog PostPublishedApril 8, 2025, 1:15 p.m....Ah, the notorious western blot: we meet again. So useful, yet so finicky to design and optimize. Today... -
Antibodies 101: Introduction to Gating in Flow Cytometry
TypeBlog PostPublishedApril 4, 2024, 1:15 p.m.... populations with basically a smear in between. Again, it can depend on the type of stain whether a tighter... -
An Integrin Antibody Toolkit from IPI
TypeBlog PostPublishedMarch 28, 2024, 1:15 p.m....αVβ3.13, may also block function of mouse αVβ3, again the first known reagent to do so. The 15 hybridoma-derived... -
Tips for Getting a Faculty Position
TypeBlog PostPublishedJuly 20, 2023, 1:15 p.m....create a new field, impact disease treatment, etc., again in clear terms. "Implications for human disease"... -
Hot Biosensors 2022: Year-End Roundup
TypeBlog PostPublishedDec. 6, 2022, 2:15 p.m....kinetics and high signal/noise ratio. Here, Takeharu Nagai and colleagues present an improved genetically encoded... -
3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells
TypeBlog PostPublishedSept. 5, 2017, 1:58 p.m....template that corrects the blocking mutation and again identify the right clone from a few hundred. From...