We narrowed to 402 results for: LARGE
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TypeBlog PostPublishedJan. 12, 2017, 3:30 p.m....Addgene’s ready-made entry clones can be used with a large variety of plasmids. Speed The Gateway system enables...
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Mesothelioma - Causes, Symptoms, and Treatment
TypeBlog PostPublishedDec. 13, 2016, 3:30 p.m....diagnosis indicate, for Stage 3, that the cancer is large in size and may have spread while Stage 4 indicates... -
Advancing Biology with Zebrafish: Genetic Tools for Developmental Studies and More
TypeBlog PostPublishedAug. 13, 2019, 12:58 p.m....Zebrafish mutants In the 1990’s, two revolutionary large-scale random mutagenesis screens produced ~1,500... -
Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor
TypeBlog PostPublishedJuly 23, 2019, 12:46 p.m....insertions and deletions (indels) most likely to have large phenotypic effects such as those that affected gene... -
To Codon Optimize or Not: That is the Question
TypeBlog PostPublishedNov. 12, 2020, 2:15 p.m.... vivo (Slimko and Lester, 2003). Addgene has a large selection of codon optimized genes, so check out... -
Tips from the Repository Trenches: Using Barcodes to Track Samples
TypeBlog PostPublishedOct. 6, 2015, 2:30 p.m....information in a small space and is ideal for tracking large collections. Many companies, such as Thermo Fisher... -
Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More
TypeBlog PostPublishedDec. 2, 2014, 7:23 p.m....been very difficult for us to respond to such a large number of requests by ourselves without Addgene.... -
Tips for Using FRET in Your Experiments
TypeBlog PostPublishedNov. 5, 2014, 3:41 p.m....for dynamic experiments in which FRET changes are large. Two other methods are commonly used to measure... -
Plasmids 101: Multicistronic Vectors
TypeBlog PostPublishedSept. 9, 2014, 8:20 p.m....have some disadvantages. These elements are quite large (500-600 bp) and may take up precious space in viral... -
Plasmids 101: The Promoter Region – Let's Go!
TypeBlog PostPublishedApril 3, 2014, 8:05 p.m....transcription factors, therefore promoters play a large role in determining where and when your gene of ... -
Using AAV for Neuronal Tracing
TypeBlog PostPublishedAug. 9, 2018, 1:04 p.m....using these viruses to mark neuronal connectivity a large majority of neuronal cells are labeled overtime ...based on its unique hue (plasmids at Addgene). A large-scale example of neuronal tracing with AAV vectors... -
QC Sequencing Technologies at Addgene
TypeBlog PostPublishedJuly 15, 2025, 1:15 p.m....constantly faces the challenge of keeping up with the large amount of QC analysis required every week. They ... -
CRISPR 101: Homology Directed Repair
TypeBlog PostPublishedJan. 26, 2023, 2:15 p.m....thus dsDNA plasmids are generally preferred for large insertions such as fluorescent proteins or selection... -
New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression
TypeBlog PostPublishedJan. 24, 2023, 2:15 p.m....difficult. The first-generation mARGs also had a large genetic footprint (two cassettes + a booster cassette... -
CRISPR-based Genome Editing Systems For Editing in E. coli
TypeBlog PostPublishedApril 13, 2021, 1:15 p.m.... linearized pEcgRNA backbone can be prepared in large amounts and frozen for future use or directly ligated... -
Plasmids 101: Plasmid Incompatibility
TypeBlog PostPublishedMarch 31, 2020, 1:15 p.m....lead to asymmetrical plasmid loss of the usually large, low copy virulence plasmids. This strategy has ...partitioning machinery away from a larger, low copy plasmid and the larger, low copy plasmid is more likely... -
Tips for CRISPR Gene Editing in Mice
TypeBlog PostPublishedJune 28, 2016, 10:59 a.m.... 3. Mashiko, Daisuke, et al. "Feasibility for a large scale mouse mutagenesis by injecting CRISPR/Cas ... -
Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View
TypeBlog PostPublishedOct. 19, 2017, 1:54 p.m....by adding a nuclear localization sequence (SV40 Large T nuclear localization sequence) and expressing ... -
pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs
TypeBlog PostPublishedDec. 14, 2017, 2:08 p.m....factors - OCT3/4, SOX2, KLF4, C-MYC, LIN28 and SV40 large T antigen (SV40LT). The method had a reprogramming...research and clinical trials, as it allows access to a larger number of willing participants. While fibroblasts... -
Deep Mutational Scanning with One Pot Saturation Mutagenesis
TypeBlog PostPublishedFeb. 22, 2017, 3:30 p.m....Measuring the Activity of Protein Variants on a Large Scale Using Deep Mutational Scanning.” Nature Protocols...