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We narrowed to 580 results for: RON

Showing: 281 - 300 of 580 results

  1. Summer SciComm Series: Master of SciComm

    Type
    Blog Post
    Published
    July 21, 2022, 1:15 p.m.
    ...interests and areas of expertise. Perhaps the strongest aspect of science communication, then, is its ...allowed to percolate within a new intellectual environment. Over time, this interest came to incorporate...

  5. Identifying Sequence Elements with SnapGene's Feature Database

    Type
    Blog Post
    Published
    Dec. 21, 2017, 2:06 p.m.
    ...possible option, we place the most important controls front and center, and make specialized controls available...plasmids were inconsistent and sometimes clearly wrong. In an effort to be rigorous, we dug into the original...

  6. New and Upcoming Viral Vectors - December 2019

    Type
    Blog Post
    Published
    Dec. 19, 2019, 2:40 p.m.
    ...with ever more efficient tools for light mediated neuronal control and we are expanding our viral service...photostimulation of axons and dendrites on neighboring neurons. We have also packaged hChR2(H134R) plasmids from...

  7. Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?

    Type
    Blog Post
    Published
    June 9, 2016, 2:30 p.m.
    ...gene-editing protein - a DNA-cleaving Argonaute from Natronobacterium gregoryi, or NgAgo for short. Addgene has ... Han. “DNA-guided genome editing using the Natronobacterium gregoryi Argonaute.” Nat Biotechnol. Epub ...

  8. Even more elegant: Single injection CRISPR/Cas9 in C. elegans

    Type
    Blog Post
    Published
    July 7, 2015, 3:36 p.m.
    ...elegans, they inserted the SEC into a synthetic intron between a fluorescent protein (FP) and a 3xFLAG...resulting loxP scar is located in this synthetic intron, and only the fluorescent protein and 3xFLAG-tag...

  9. The Importance of High Titer for AAV Transductions

    Type
    Blog Post
    Published
    March 1, 2018, 2:11 p.m.
    ...for successful transduction. In another example, neuronal gene expression by an anterograde AAV was shown...input-defined neural pathways for defense behaviors." Neuron 93.1 (2017): 33-47. PubMed PMID: 27989459. PubMed...

  10. Using CRISPR/Cas9 to Edit Disease Out of the Genome

    Type
    Blog Post
    Published
    June 23, 2022, 1:15 p.m.
    ...disease mutation in basal forebrain cholinergic neurons derived from iPSCs. From Rohn et al. 2018; see ... Limitations, and Implications for the Future.” Front. Oncol. https://doi.org/10.3389/fonc.2020.01387 ...

  12. Visualizing Genomic Loci with CRISPR-Sirius

    Type
    Blog Post
    Published
    April 18, 2019, 12:53 p.m.
    ...CRISPRainbow. Both tools were targeted to the FBN3 intronic repeat which contains 22 copies of the gRNA target...distances of loci pairs from intergenic regions, intronic regions, and a pericentromeric region. All pairs...

  13. Improving Transduction Efficiency with Exosome AAVs

    Type
    Blog Post
    Published
    March 14, 2019, 1:54 p.m.
    ...cells (as demonstrated in different cellular environments such as retina or glioblastoma). They also found.... Exo-AAV9 was successfully shown to transduce neurons in the brain after systemic (i.v.) delivery in ...

  14. K. phaffii: Rising to the Occasion

    Type
    Blog Post
    Published
    April 4, 2023, 1 p.m.
    ...generating knock-in mutations, and NHEJ is an error-prone pathway frequently introducing frameshift knockout...Emerging Model Organisms in Fundamental Research. Front. Microbiol. 11-2020, 2021. DOI: 10.3389/fmicb.2020.607028...

  15. Pushing the Limits of DNA Assembly

    Type
    Blog Post
    Published
    Sept. 6, 2017, 1:01 p.m.
    ...Assembly method from New England Biolabs® has minimal upfront requirements and allows you to expediently join...-executed cloning endeavor, NEBuilder requires upfront planning. This cloning methodology requires that...

  17. How Dry Ice Affects Viral Vector Sample pH & How to Avoid It

    Type
    Blog Post
    Published
    June 20, 2017, 2:30 p.m.
    ...shipped), where they would be exposed to a closed environment containing CO2 (from the dry ice). After 48 hours...PMID: 23538862. 2. Potter M, Lins B, Mietzsch M, Heilbronn R, Van Vliet K, Chipman P, Agbandje-McKenna M,...

  18. Addgene Depositors Get More Citations

    Type
    Blog Post
    Published
    April 20, 2017, 2:30 p.m.
    ...10.5%  3.2  534.1 3 2010 3 Applied and Environmental Microbiology  1.6%  1.2% 0.75   2.3 4 2010... 2010 167  Applied and Environmental Microbiology  1.6% 1.2%  0.75   125.3 In figure 2, you can...

  19. Optimizing Donor DNA for Enhanced CRISPR Genome Editing

    Type
    Blog Post
    Published
    March 24, 2016, 2:30 p.m.
    ...number, but it was attained without any of the error-prone repair that normally accompanies Cas9 cutting. We... holds some kind of fitness advantage but error-prone repair of the gene would be disastrous (i.e. if ...

  20. Management for Scientists: Delegating is Key

    Type
    Blog Post
    Published
    Feb. 20, 2015, 8:30 p.m.
    ...effective delegation skills: The team will be stronger and less dependent on the manager to succeed. ... your team and take responsibility if things go wrong. Here are some important criteria for delegating...
Showing: 281 - 300 of 580 results