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Showing: 281 - 300 of 809 results
  1. Viral Vectors 101: What is a Virus (and a Viral Vector)?

    Type
    Blog Post
    Published
    Aug. 18, 2023, 4:15 p.m.
    ...done to and for us? BMC Biology, 15, 91. https://doi.org/10.1186/s12915-017-0433-z. PMID: 29052511. Louten...Replication. Essential Human Virology, 49. https://doi.org/10.1016/B978-0-12-800947-5.00004-1 Taylor, M. W...40). Springer International Publishing. https://doi.org/10.1007/978-3-319-07758-1_2 More resources on the...
  2. What the HEK?

    Type
    Blog Post
    Published
    April 18, 2023, 1:15 p.m.
    ... etc.) and are ready to use on another cell or organism. This pipeline is utilized to transduce cell lines...resources on the Addgene blog Five Popular Model Organisms 10 Tips for Mammalian Cell Culture Adenoviral...and Troubleshooting Additional resources on Addgene.org AAV Production in HEK-293T Cells  ...
  3. Identifying Sequence Elements with SnapGene's Feature Database

    Type
    Blog Post
    Published
    Dec. 21, 2017, 2:06 p.m.
    ...feature database. Powering the plasmid maps on Addgene.org with SnapGene Server is useful because it helps...is to enhance Collections so that a group or organization can annotate their sequences from a shared database...sequencing at Addgene Additional Resources on Addgene.org Example plasmid analysis powered by SnapGene...
  4. Science Careers: Unruly Interests Feed Many Paths

    Type
    Blog Post
    Published
    Sept. 26, 2017, 12:12 p.m.
    ...fascinating biology is found in weird and rare organisms so I found it hard to stick to vertebrates alone...afield at first and I let them pass by. But I didn’t forget: what would it be like? Then I spotted an opening...Subscribe to Career Blog Posts Resources at Addgene.org Find Job Openings at Addgene ...
  5. Behind-the-scenes of the Isolation of the Thermostable IgnaviCas9 From a Yellowstone Hot Spring

    Type
    Blog Post
    Published
    Nov. 12, 2019, 2 p.m.
    ...abundant biological dark matter and so the lab organized a sampling trip to Yellowstone National Park (...then, many researchers have searched for novel organisms and enzymes from these extreme environments. Gearing...GeoCas9, another thermostable Cas9 Resources on Addgene.org Read our CRISPR guide Find CRISPR plasmids for...
  6. Rewiring Metabolic Circuitry with CRISPR RNA Scaffolds [Video]

    Type
    Blog Post
    Published
    April 7, 2015, 4:21 p.m.
    ...Plasmids for CRISPRi are described here: http://www.addgene.org/crispr/qi/ Plasmids described in  Zalatan...al. Cell  (2015) can be found here: https://www.addgene.org/browse/article/9593/ Plasmids described in...al. Cell  (2013) can be found here: https://www.addgene.org/browse/article/6441/ Resources: An open source...
  7. Revamp Your Lab Meetings With Creative Virtual Collaboration

    Type
    Blog Post
    Published
    Feb. 9, 2021, 2:15 p.m.
    ... paper. PLoS Comput Biol 14:e1006508 . https://doi.org/10.1371/journal.pcbi.1006508 Vicens Q, Bourne PE...Collaboration. PLoS Comput Biol 3:e44 . https://doi.org/10.1371/journal.pcbi.0030044 Wuchty S, Jones BF... of Knowledge. Science 316:1036–1039 . https://doi.org/10.1126/science.1136099  ...
  8. FlipGFP, a novel fluorescence protease reporter to study apoptosis

    Type
    Blog Post
    Published
    May 21, 2019, 12:10 p.m.
    ...cleavage. Secondly, fluorescence imaging of living organisms can be challenging due to issues with tissue autofluorescence...dynamics of apoptosis both in vitro and in living organisms to better study and understand these diseases ...Read our plasmids 101 blog series Resources on Addgene.org Browse the fluorescent protein collection Find...
  9. Addgene-Seeding Labs plasmid grant round 3: microbial drug resistance and biofilms, antidiabetic plant components, visualizing brain injury - and a glowing robot fish

    Type
    Blog Post
    Published
    Nov. 27, 2018, 2 p.m.
    ...extreme life on earth including extremophile microorganisms, biomarkers, and recently, cancer research ...Addgene has partnered with various community organizations and workforce projects to reach audiences beyond...Previous Awardee Kwabena Duedu Resources at Addgene.org Check out our CRISPR resources and CRISPR for...
  10. Unique and persistent IDs for improved reproducibility: Addgene now supports RRIDs and compact identifiers for all plasmids

    Type
    Blog Post
    Published
    Nov. 20, 2018, 8:17 p.m.
    ...identification of research reagents, several journals or organizations now recommend or require authors to use specific...well-established prefix resolution services such as Identifiers.org and N2T.net. To help authors comply with journal...Addgene_87155” and the following URL (https://scicrunch.org/resolver/RRID:Addgene_87155) will take you directly...
  11. Science Career Options

    Type
    Blog Post
    Published
    Feb. 23, 2017, 1:30 p.m.
    ...that requires travel can show that you are self organised, reliable, and can work independently. Practical...involves traveling, showing that you are a highly organised and experienced traveler by citing specific examples...the Contacts for Your Dream Job Resources on Addgene.org Addgene Careers Page ...
  12. Plasmids 101: Gram Negative and Positive Bacteria

    Type
    Blog Post
    Published
    Aug. 2, 2022, 1:15 p.m.
    ...American Society for Microbiology (2005). https://asm.org/getattachment/5c95a063-326b-4b2f-98ce-001de9a5ece3.... Cleveland Clinic. Gram Stain. https://my.clevelandclinic.org/health/diagnostics/22612-gram-stain Hardy...
  13. DAP arrays for multiplex gene editing

    Type
    Blog Post
    Published
    July 7, 2022, 1:15 p.m.
    ...multiplex gene editing in a variety of cells and organisms. However, lengthy genetic payloads and insufficient...processing mechanism, which is conserved in all living organisms and has previously been used for multiplex Cas9...nuclease editing, in virtually any cell type or organisms of interest with only minimal genetic payloads...
  14. Networking Follow-up: I Met a Person. Now What?

    Type
    Blog Post
    Published
    Jan. 12, 2021, 2:15 p.m.
    ...never forget the strawberries. Grand gestures are memorable! Attend events for one organization or go ...If appropriate, invite them to speak for your organization, mentoring group, or department. Even if it ...
  15. Management for Scientists: Delegating is Key

    Type
    Blog Post
    Published
    Feb. 20, 2015, 8:30 p.m.
    ...action plans! As a project progresses it is easy to forgot the original plan. Write down your plans in a shared...develop to reach their full potential in the organization. This will result in more engaged, successful...Series Learn How Volunteering at a Professional Organization Can Help You Develop Leadership Skils Set Concrete...
  16. 15 Hot Plasmids from 2017

    Type
    Blog Post
    Published
    Jan. 9, 2018, 3:02 p.m.
    ... using a viral approach Genetically modified organisms like transgenic mice are important tools for understanding...specific to interneurons in mice as well as other organisms, opening the possibility of using these tools ...protein (mCyRFP1). Derived from cyan-excitable organge fluorescent protein (CyOFP1), some of the exciting... a stable monomeric form as determined by the organized smooth endoplasmic reticulum (OSER) assay. A major...
  17. GCE4All: Making Genetic Code Expansion Accessible

    Type
    Blog Post
    Published
    June 15, 2023, 1:15 p.m.
    ...Proteins. Science, 244(4901), 182–188. https://doi.org/10.1126/science.2649980 Shandell, M. A., Tan, Z...Perspective. Biochemistry, 60(46), 3455–3469. https://doi.org/10.1021/acs.biochem.1c00286 Zhu, P., Mehl, R., ... Proteins in Escherichia coli. 12(21). https://doi.org/10.21769/BioProtoc.4541  ...
  18. Viral Vectors 101: Viral Applications

    Type
    Blog Post
    Published
    April 13, 2023, 1:15 p.m.
    ...development. Vaccines. 7, 75 (2022). DOI: https://doi.org/10.1038/s41541-022-00503-y Gorry PR, McPhee DA...Transduct Target Ther 6, 53 (2021). DOI: https://doi.org/10.1038/s41392-021-00487-6 Additional Resources...Editing to Primary Cells Additional Resources on Addgene.org Browse Our Viral Vector Pages Learn About Viral...
  19. Sharing is Caring: But How to Distribute Open Hardware?

    Type
    Blog Post
    Published
    Feb. 28, 2023, 2:15 p.m.
    ...disciplinary boundaries and can allow for innovative organizations and companies to provide problem-solving platforms...microscopic imaging. Nat Commun 11, 5979 (2020). https://doi.org/10.1038/s41467-020-19447-9 Hohlbein, J., Diederich...vadis?. Nat Methods 19, 1020–1025 (2022). https://doi.org/10.1038/s41592-022-01602-3...
Showing: 281 - 300 of 809 results