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  1. Choosing a Good Mentor for Scientists

    Type
    Blog Post
    Published
    Jan. 16, 2014, 2:56 p.m.
    ...the lab, if possible. Attend a lab meeting. Spend some time hanging out with members of the lab. It is ...There are many excellent mentors out there. With some good pre-work you can find a lab that will deserve...

  2. New and Upcoming Viral Vectors - December 2019

    Type
    Blog Post
    Published
    Dec. 19, 2019, 2:40 p.m.
    ...inventory of ready-to-use viral vectors. Here are some of the AAV we have released in the last few months...Harvey’s lab. These plasmids localize opsins to the soma to avoid off target photostimulation of axons and...

  4. Summer SciComm Series: Master of SciComm

    Type
    Blog Post
    Published
    July 21, 2022, 1:15 p.m.
    ...lab reports and empirical essays had also stirred some of my more creative faculties into motion, such .... Often, these companies believe they possess something that will truly benefit others. Herein, a good...

  5. RUBY-Red Siliques

    Type
    Blog Post
    Published
    March 1, 2022, 2:15 p.m.
    ...is great for a wide variety of applications. But some techniques require you to select against the presence...studying large samples. RUBY Red But recently, something new has been added to the palette. RUBY is a bright...

  6. Hot Plasmids and Viral Preps - September 2021

    Type
    Blog Post
    Published
    Sept. 21, 2021, 1:15 p.m.
    ...our CRISPR Plasmids and Resources page. Here are some highlights from the past couple months including...collection to provide ready-to-use viral preps. Here are some of the new viral preps from recent months: Second...

  7. Antibodies 101: Avoiding the Mouse on Mouse Mess in IHC

    Type
    Blog Post
    Published
    Sept. 21, 2023, 1:15 p.m.
    ...will have the same levels of endogenous IgGs, so some researchers can get away with species on species...primary and secondary antibody complexes (Figure 2B) Some labs have found that you can significantly decrease...

  8. Identifying Sequence Elements with SnapGene's Feature Database

    Type
    Blog Post
    Published
    Dec. 21, 2017, 2:06 p.m.
    ... in commercial plasmids were inconsistent and sometimes clearly wrong. In an effort to be rigorous, we...we dug into the original literature, some of it decades old, to provide reliable feature annotations. ...

  9. 10 Great Guest Posts We're Thankful For

    Type
    Blog Post
    Published
    Nov. 18, 2016, 3 p.m.
    ...to show our gratitude on the blog by highlighting some of the great posts written by Addgene depositors...-body mutants that respond to different pH’s and some that are fused to fluorescent proteins. She also...

  13. New and Upcoming Viral Vectors - September 2019

    Type
    Blog Post
    Published
    Sept. 12, 2019, 1 p.m.
    ...inventory of ready-to-use viral vectors. Here are some of the AAV we have released in the last few months...manipulating specific neurons. We recently packaged a soma-targeted opsin from Christopher Harvey’s lab. According...

  14. Plasmids 101: Gram Negative and Positive Bacteria

    Type
    Blog Post
    Published
    Aug. 2, 2022, 1:15 p.m.
    ...realized that certain stains were retained only in some species of bacteria. The stain colored one type,...medicine and more successful experiments. It’s something that all scientists should understand when stepping...

  15. Using CRISPR/Cas9 to Edit Disease Out of the Genome

    Type
    Blog Post
    Published
    June 23, 2022, 1:15 p.m.
    ...that CRISPR/Cas9 could be used to edit several autosomal-dominant mutations that have been connected to...disease in the near future,” Li says, though there are some things to sort out first. The potential for off-...

  17. Addgene Depositors Get More Citations

    Type
    Blog Post
    Published
    April 20, 2017, 2:30 p.m.
    ...journals as those that did make deposits to Addgene.  Insomuch as different journals represent different research...using these weighted numbers. Note: this does drop some journals from our dataset, since not all journals...

  18. The MTA at Addgene

    Type
    Blog Post
    Published
    July 23, 2024, 2:17 p.m.
    ...often get positive feedback on “our” MTAs, which sometimes makes people think our MTAs are different than... research use only to industry-based scientists. Some organizations choose to share their plasmids under...

  19. Predicting Adverse Reactions to Monoclonal Antibody Drugs

    Type
    Blog Post
    Published
    Feb. 13, 2024, 2:15 p.m.
    ...BY fromGroell et.al, 2018.   3D models are somewhat of a middle ground between 2D assays and in vivo...of the adverse drug reaction. This system would somewhat act like a 2-step verification. All approaches...

  20. Summer SciComm Series: A PhD in Science Communication

    Type
    Blog Post
    Published
    Aug. 23, 2022, 1:15 p.m.
    ... an experiment, then spend the evening listen to some bloke at the pub telling me that actually he drives...might struggle to understand. Why should we trust something that we cannot read or verify for ourselves? Further...

  22. Optimizing Donor DNA for Enhanced CRISPR Genome Editing

    Type
    Blog Post
    Published
    March 24, 2016, 2:30 p.m.
    ...Our recent paper (Richardson et al 2016) revealed some of the biophysical parameters that can influence...genetic diseases in which sequence replacement holds some kind of fitness advantage but error-prone repair...

  23. Components of CRISPR/Cas9

    Type
    Blog Post
    Published
    Feb. 2, 2016, 5 p.m.
    ...' NAAAAC  The synthetic guide RNA or gRNA (sometimes sgRNA) In the native Type II CRISPR/Cas system...sequence) to modify the desired genomic locus.   Some historical notes CRISPR arrays in bacterial genomes...

  24. Addgene Welcomes John Doench to the Advisory Board

    Type
    Blog Post
    Published
    Aug. 13, 2015, 2:30 p.m.
    ...assignment that was open ended... it was actually something bordering on research, rather than 'here’s the..., sharing ethic that Addgene has behind it is something that very much resonates with me.” About Addgene...

  25. Plasmids 101: Mammalian Vectors

    Type
    Blog Post
    Published
    March 25, 2014, 3:15 p.m.
    ...a process called transfection. Transfection is somewhat comparable to bacterial transformation (the introduction...large-T antigen (293E or 293T cells), allow for episomal amplification of plasmids containing the viral...

  26. Plasmids 101: Screening Strategies Used in Plasmid Cloning

    Type
    Blog Post
    Published
    Sept. 15, 2020, 1:15 p.m.
    ...Find plasmids for blue-white screening at Addgene! Some bacterial strains contain lacZ in their genome, ...sequencing primers in our Molecular Biology Reference. Sometimes common  primers can't provide all of the information...
Showing: 451 - 480 of 745 results