We narrowed to 762 results for: SOM

...eluting in water, Tris-EDTA (TE), just Tris buffer, or some other variations. Does it make a difference? Short...helix, fragmenting plasmid DNA, PCR product, or chromosome. One of the main causes of degradation during...

...gastrointestinal tract. Require Administration of Substrate: Some luciferase reporters, such as Renilla luciferase...imaging shows only that the bacteria localize to somewhere in the mouse abdomen. arg1 signal, however, can...

...confirm the sequence of a plasmid and we’ll highlight some of the new features available on our website through...experiments: Primer and restriction digest design Some regions of plasmids are difficult to sequence by...

...can indicate behavioral state, for example, if someone is awake, agitated, or asleep. Though DA firing...concentration. To address this, researchers from some labs have developed fluorescent-protein based DA...

...manipulation tools  Sometimes DNA sequences need to be “cleaned up” or changed in some way before you can...

...our CRISPR Plasmids and Resources page. Here are some highlights from the past couple months: A new lentiviral...collection to provide ready-to-use viral preps. Here are some of the new viral preps from recent months: Our ...

...hydrogen peroxide or ions like calcium and zinc. Some biosensors contain a single fluorescent protein ...information you are looking for. Below we have summarized some of the common fluorescent detection methods and ...

... processes. Initially, Tanenbaum et al. observed some GFP aggregation, which they reduced by using superfolder...concerns are greater with SunTag. v1 SunTag exhibits some scaffold aggregation (v4 Suntag does not.) It’s...

...advantages of viruses and naked DNA while eliminating some of the drawbacks. Specifically, viruses are able...avoids many, though not all, of these defenses. For some applications of genome engineering - such as certain...

...Chris Voigt, another Addgene depositor, discussed some of the many challenges of creating biological circuits...repository, and I was proud to be responsible for sharing some of our data at this meeting. We weren't quite sure...

... that aligns with your interests. We've outlined some important factors to consider and summarize helpful... “not be shackled by your own subject - maybe something used in astrophysics can be applied to fungal ...

...the lab, if possible. Attend a lab meeting. Spend some time hanging out with members of the lab. It is ...There are many excellent mentors out there. With some good pre-work you can find a lab that will deserve...

...powerful tool to study proteins in vivo, it does have some limitations. GFP has long protein maturation times...amino acids, thus it is translated quickly by the ribosome. This feature provides HA frankenbody the ability...

...vectors (AAV) not all serotypes are equal; while some serotypes are typically high yielding, for others...Clathrin-mediated endocytosis and subsequent endo-lysosomal trafficking of adeno-associated virus/phage." ...

...will have the same levels of endogenous IgGs, so some researchers can get away with species on species...primary and secondary antibody complexes (Figure 2B) Some labs have found that you can significantly decrease...

...lab reports and empirical essays had also stirred some of my more creative faculties into motion, such .... Often, these companies believe they possess something that will truly benefit others. Herein, a good...

...is great for a wide variety of applications. But some techniques require you to select against the presence...studying large samples. RUBY-Red But recently, something new has been added to the palette. RUBY is a bright...

...our CRISPR Plasmids and Resources page. Here are some highlights from the past couple months including...collection to provide ready-to-use viral preps. Here are some of the new viral preps from recent months: Second...

...to show our gratitude on the blog by highlighting some of the great posts written by Addgene depositors...-body mutants that respond to different pH’s and some that are fused to fluorescent proteins. She also...

... in commercial plasmids were inconsistent and sometimes clearly wrong. In an effort to be rigorous, we...we dug into the original literature, some of it decades old, to provide reliable feature annotations. ...

...inventory of ready-to-use viral vectors. Here are some of the AAV we have released in the last few months...Harvey’s lab. These plasmids localize opsins to the soma to avoid off target photostimulation of axons and...

...often get positive feedback on “our” MTAs, which sometimes makes people think our MTAs are different than... research use only to industry-based scientists. Some organizations choose to share their plasmids under...

...your DNA is intact or the correct size. This is something that absorbance based methods can’t tell you and...ELISA reader if no other instruments are available. Some last reminders about quantifying DNA In choosing...

...technology before the conference begins.“There were some concerns of having a stable internet connection,...We made sure that we had a backup plan in case someone’s internet cut out. “ On networking Networking can...

...inventory of ready-to-use viral vectors. Here are some of the AAV we have released in the last few months...manipulating specific neurons. We recently packaged a soma-targeted opsin from Christopher Harvey’s lab. According...

...BY fromGroell et.al, 2018.   3D models are somewhat of a middle ground between 2D assays and in vivo...of the adverse drug reaction. This system would somewhat act like a 2-step verification. All approaches...

... an experiment, then spend the evening listen to some bloke at the pub telling me that actually he drives...might struggle to understand. Why should we trust something that we cannot read or verify for ourselves? Further...

...realized that certain stains were retained only in some species of bacteria. The stain colored one type,...medicine and more successful experiments. It’s something that all scientists should understand when stepping...

...that CRISPR/Cas9 could be used to edit several autosomal-dominant mutations that have been connected to...disease in the near future,” Li says, though there are some things to sort out first. The potential for off-...

...contributions.   Our volunteers are extremely diverse. Some of them are undergraduates or PhD students who are...researcher makes a small but crucial contribution to someone else’s project. We think that these collaborations...