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  1. An Inside Look into Addgene's Dev Team

    Type
    Blog Post
    Published
    Jan. 23, 2020, 2:45 p.m.
    ...Addgene blog Learn about what went into our search engine update Find all Addgene news Resources on Addgene.org... Sophia K. Cheng...

  2. Lab to Office Culture Shock

    Type
    Blog Post
    Published
    Feb. 5, 2019, 1:04 p.m.
    ...counter-intuitive, but I find office work more mentally challenging than lab work at times.” Sometimes a desk job...to non-scientists can be a fun and gratifying challenge. You’re also getting the opportunity to learn ...

  3. Supporting Reproducibility with a Connected ELN

    Type
    Blog Post
    Published
    Jan. 17, 2019, 1:38 p.m.
    ...response to requirements that emerged from intensive engagement with a wide range of people at the University... The two guiding principles emerging from the engagement with the Wisconsin research community were to...

  4. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

    Type
    Blog Post
    Published
    Nov. 1, 2018, 12:35 p.m.
    ...Central PMCID: PMC384129. Aizenman, Einat, Hanna Engelberg-Kulka, and Gad Glaser. "An Escherichia coli chromosomal...PMID: 12718874. Yang, Junjie, Weihong Jiang, and Sheng Yang. "mazF as a counter-selectable marker for unmarked...

  5. Save Time with Transient Plant Leaf Transformations

    Type
    Blog Post
    Published
    July 25, 2019, 12:03 p.m.
    .... During a transient leaf transformation, the engineered Agrobacterium strain is brought into contact ..., 2006. 225-229. PubMed PMID: 16739580. Li, Jian-Feng, et al. "The FAST technique: a simplified Agrobacterium-based...

  6. Bioinformatics at Addgene

    Type
    Blog Post
    Published
    July 14, 2022, 1:15 p.m.
    ...on-site, but now we were presented with a new challenge: automating the bioinformatics steps needed to... reviewing and testing several platforms for engineering pipelines, we selected Nextflow, a workflow management...

  7. Tips for CRISPR Gene Editing in Mice

    Type
    Blog Post
    Published
    June 28, 2016, 10:59 a.m.
    ...multiple genes by CRISPR/Cas-mediated genome engineering." Cell 153.4 (2013): 910-918. PubMed PMID: 23643243...Addgene Blog Get the Basics of Mouse Genome Engineering with CRISPR Validate Your Genome Edit Learn about...

  8. Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

    Type
    Blog Post
    Published
    Oct. 27, 2015, 3 p.m.
    ... cytoplasm. Generally, FPs evolved in or were engineered for use in cytoplasmic environments. However,...problems of popular fluorescent proteins and were re-engineered by mutating cysteine residues and N-glycosylation...

  9. Plasmids 101: E. coli Strains for Protein Expression

    Type
    Blog Post
    Published
    Feb. 10, 2015, 3:06 p.m.
    ...suited for recombinant protein expression. Many challenges can arise when over-expressing a foreign protein...recombinant proteins are typically expressed in E. coli engineered to accomodate high protein loads using inducible...

  10. Tips for Using FRET in Your Experiments

    Type
    Blog Post
    Published
    Nov. 5, 2014, 3:41 p.m.
    ...linking the quantity of absorbed light, at a given wavelength, to the concentration of fluorophore in solution...method, where the donor is excited by a specific wavelength of light and the signal is collected by using...

  11. Antibodies 101: Introduction to Gating in Flow Cytometry

    Type
    Blog Post
    Published
    April 4, 2024, 1:15 p.m.
    ...(see Figure 2). H describes the maximum signal strength, while W results from the time that the cell spent...increase the electric current and thereby the signal strength. So, we need to adjust the voltage in the cytometer...

  12. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    Published
    Sept. 28, 2023, 1:15 p.m.
    ...used here could be an attractive strategy for engineering other enzymes. Conclusion We hope this post has...precise control over vascular endothelial barrier strength. eLife, 12, RP84364. https://doi.org/10.7554/eLife...

  13. CRISPR 101: Validating Your Genome Edit

    Type
    Blog Post
    Published
    Nov. 3, 2022, 12:15 p.m.
    ...sites? Never fear! You can introduce a silent, passenger edit to serve as a proxy for your edit of interest... a version of Cas9 that has been specifically engineered for reduced off target editing, such as SpCas9...

  15. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    Published
    Oct. 5, 2021, 1:15 p.m.
    ...photoreceptors (Oliinyk et al., 2019). They were engineered to incorporate BV. Their advantages are their...Verkhusha, V.V. Near-infrared fluorescent proteins engineered from bacterial phytochromes. Curr Opin Chem Biol...

  16. Five Popular Model Organisms, Part 2

    Type
    Blog Post
    Published
    Sept. 29, 2020, 1:15 p.m.
    ... perfect for molecular cloning and metabolic engineering.  The E. coli genome has an extensive research...you are working with Arabidopsis check out the GreenGate Toolkit which allows for easy cloning of constructs...

  17. 3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells

    Type
    Blog Post
    Published
    Sept. 5, 2017, 1:58 p.m.
    ...References 1. Cong, Le, et al. "Multiplex genome engineering using CRISPR/Cas systems." Science 339.6121 (...Mali, Prashant, et al. "RNA-guided human genome engineering via Cas9." Science 339.6121 (2013): 823-826. ...

  18. Site Directed Mutagenesis by PCR

    Type
    Blog Post
    Published
    Aug. 2, 2016, 2:30 p.m.
    ...validation PCR followed by Restriction Fragment Length Polymorphism (RFLP) analysis (right). Introduction... absence) of that particular site with fragment length polymorphism (RFLP) analysis. In this process, ...

  19. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

    Type
    Blog Post
    Published
    April 24, 2015, 2:39 p.m.
    ...First, the imaging of Brainbow mouse tissue was challenging due to low fluorescence intensity, caused partially...Barry R, Mahou P, Le Franc Y, Supatto W, Matho KS, Ieng S, Fouquet S, Dupin E, Benosman R, Chédotal A, Beaurepaire...

  20. Viral Vectors 101: AAV Serotypes and Tissue Tropism

    Type
    Blog Post
    Published
    Jan. 16, 2025, 3:42 p.m.
    ...on. In addition, researchers have created many engineered serotypes by mixing, matching, and modifying ...summarize briefly: AAVrg is derived from AAV2 and engineered to infect neurons and undergo retrograde transport...

  21. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    Published
    June 1, 2023, 1:15 p.m.
    ..., Pillay, S., Puschnik, A. S., Nagamine, C. M., Cheng, F., Qiu, J., Carette, J. E., & Vandenberghe, L..... E., & Manfredsson, F. P. (2017). Rationally Engineered AAV Capsids Improve Transduction and Volumetric...

  22. Is this the right place for me? 8 tactics for choosing a lab

    Type
    Blog Post
    Published
    Oct. 2, 2018, 12:56 p.m.
    ... you? Maybe you like things a bit hectic and challenging and this drives your best work. Or is it teamwork... comparison can be very helpful in uncovering strengths and weaknesses of the options. Use this blog as...

  23. Transferable Skills Guide: Teamwork

    Type
    Blog Post
    Published
    Nov. 28, 2017, 2 p.m.
    ...are currently about 5.5 million articles in the English version of Wikipedia alone. Humans have been forced...a team is a really good way of creating and strengthening those social bonds. So never mind how much better...

  24. Plasmids 101: Using Transposons in the Lab

    Type
    Blog Post
    Published
    July 6, 2021, 1:28 p.m.
    ...Sleeping Beauty, piggyBac and Tol2 for Genome Engineering. IJMS 22:5084. https://doi.org/10.3390/ijms22105084...about the INTEGRATE system for bacterial genome engineering  Read about the Sleeping Beauty DNA transposon...

  25. Antibodies 101: Validation

    Type
    Blog Post
    Published
    March 24, 2022, 1:15 p.m.
    ... signal, so you’ll need a range of expression strengths to be confident in your data. This is a good option...9. https://doi.org/10.1038/518027a  Roncador G, Engel P, Maestre L, Anderson AP, Cordell JL, Cragg MS,...

  27. A Case Study of Addgene Bioinformatics

    Type
    Blog Post
    Published
    May 25, 2023, 1:15 p.m.
    ...our pipeline. In it, they break down the unique challenges of our platform and how we were able to address...

  29. CRISPR Guide Update

    Type
    Blog Post
    Published
    Oct. 10, 2024, 1:15 p.m.
    ...the Cascade-Cas3 system. We also describe newly engineered Cas9s with more flexible requirements and enhanced...
Showing: 451 - 480 of 672 results