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We narrowed to 814 results for: org

Showing: 451 - 480 of 814 results
  1. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

    Type
    Blog Post
    Published
    June 2, 2015, 6:06 p.m.
    ...processing machinery present in almost every living organism. Precursor, or pre-tRNAs are cleaved by RNase ... illustrated the power of polycistronic gene organization, and its translation to CRISPR/Cas9 shows great...
  2. Gendered Innovations: Why Does Sex of the Cell Matter?

    Type
    Blog Post
    Published
    July 21, 2016, 2:30 p.m.
    ...components of biological research that are often forgotten or ignored. If we wish to conduct research that...careers through structural change in research organizations. “Fix the Knowledge” or “gendered innovations...
  3. Antibodies 101: Affinity Reagents

    Type
    Blog Post
    Published
    April 14, 2022, 1:15 p.m.
    ...Introduction to Antibodies Additional resources on addgene.org  Antibody GuideRecombinant antibodies available...Format. Journal of Biological Chemistry. https://doi.org/10.1074/jbc.M407888200 Kwon, N., Kim, Y., Lee, ...
  4. RaPID Detection of RNA-protein Interactions

    Type
    Blog Post
    Published
    Aug. 16, 2018, 12:40 p.m.
    ...alternative option but it’s toxic to tissues and model organisms. Detecting RNA-protein interactions with RaPID...resources for studying chromatin Resources on Addgene.org Find Split-BioID plasmids Find CRISPR plasmids...
  5. CRISPRainbow and Genome Visualization

    Type
    Blog Post
    Published
    Feb. 28, 2017, 3:30 p.m.
    ...helping researchers visualize the genome and its organization within the nucleus, also called the 4D nucleome...Control Protein Activity with GFP Resources on Asddgene.org Find Fluorescent Protein Plasmids Find Fluorescent...
  6. Viral Vectors 101: An Introduction to AAV

    Type
    Blog Post
    Published
    May 30, 2023, 1:15 p.m.
    ...types even when systemically administered in an organism. These features make AAV ideal for human therapies...Learn about Viral Vector Elements Resources on Addgene.org Read Our AAV Guide Find AAV Vectors Browse All...
  7. The time and cost required to make a plasmid

    Type
    Blog Post
    Published
    March 23, 2023, 1:15 p.m.
    ...where to find them! Keeping good records and an organized lab saves time and money across the board. Consider...Download the Plasmids 101 eBook Resources on Addgene.org Join the community of Addgene depositors here...
  8. Troubleshooting Your Plasmid Cloning Experiment

    Type
    Blog Post
    Published
    Sept. 24, 2019, 12:55 p.m.
    ...want to amplify sequences from a large genome organisms like mouse or human. There are several online ...about different plasmid elements Resources on Addgene.org Find protocols for plasmid cloning Read our ...
  9. Plasmid Tools for Microbiome Studies

    Type
    Blog Post
    Published
    June 27, 2019, 12:25 p.m.
    ...Sequencing the 16S rRNA reveals the identity of the organisms present while sequencing of all transcripts gives...of our Plasmids 101 blog posts Resources on Addgene.org Check out our microbiology resource page Deposit...
  10. Addgene: The First Twenty Years

    Type
    Blog Post
    Published
    Dec. 19, 2024, 2:15 p.m.
    ...old-fashioned way: through physical outreach, visiting organizations, knocking on doors, and attending conferences...the globe.  “Read the Addgene guide: https://www.addgene.org/guides/crispr/” — SirHamhands, responding to...
  11. Plasmids 101: Cre-lox

    Type
    Blog Post
    Published
    Jan. 13, 2015, 3:47 p.m.
    ...including activating an oncogene only in a particular organ, or bypassing embryonic lethality. To facilitate... K. 1993. PubMed PMID: 8513499. Resources on Addgene.org Find Cre plasmids Find loxP plasmids  ...
  12. Transferable Skills Guide: Cross-team Communication

    Type
    Blog Post
    Published
    Nov. 21, 2019, 1:53 p.m.
    ...sample,” did the word convey the same concept to Morgan, our senior engineer? The answer to these questions...science communication blog posts Resources on Addgene.org Find careers at Addgene ...
  13. 6 Tips for Grant Writing

    Type
    Blog Post
    Published
    Oct. 18, 2016, 2:30 p.m.
    ...used to conversing with other scientists that we forget that some of the terminology we use routinely isn...applications for funding from charities, commercial organizations, or universities may have someone senior from...
  14. Tips for Technical Support Calls

    Type
    Blog Post
    Published
    Sept. 20, 2016, 2:39 p.m.
    ... the company’s FAQs? A lot of companies and organizations selling biological reagents have a ton of data...with the Addgene Deposit Process Resources on Addgene.org Check Out Our Frequently Asked Questions Pages...
  15. Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux

    Type
    Blog Post
    Published
    Sept. 13, 2016, 2:30 p.m.
    ...Advocating for Science Symposium and Workshop, organized by Future of Research, Academics for the Future... More information can be found at http://futureofresearch.org/advocating-for-science-boston-2016/. Sign...
  16. Adapting Toehold Switches to Detect Zika Virus

    Type
    Blog Post
    Published
    Aug. 30, 2016, 2:30 p.m.
    ...Guillain-Barré syndrome. In response, the World Health Organization has declared a global health emergency and called...Used to Study Dangerous Viruses Resources on Addgene.org Check out All the Plasmids from the Collin's...
  17. 5 Tips for Troubleshooting Viral Transductions

    Type
    Blog Post
    Published
    Aug. 11, 2016, 2:23 p.m.
    ...PMID: 12036457. 6. Davis, Howard E., Jeffrey R. Morgan, and Martin L. Yarmush. "Polybrene increases retrovirus...Lentiviral Vectors Additional Resources on the Addgene.org Check out Our Viral Vector Guide Pages Find ...
  18. Corralling and Cornering the Goldilocks Antibody

    Type
    Blog Post
    Published
    Aug. 9, 2022, 1:15 p.m.
    ...in research. Nature 518, 27–29 (2015). https://doi.org/10.1038/518027a Freedman LP, Cockburn IM, Simcoe...Preclinical Research. PLoS Biol 13(6): e1002165. https://doi.org/10.1371/journal.pbio.1002165 More resources on ...
  19. Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

    Type
    Blog Post
    Published
    Aug. 24, 2021, 1:15 p.m.
    .... Mol. Life Sci. 76, 4689–4704 (2019). https://doi.org/10.1007/s00018-019-03239-6 Additional resources...in our Intro to Antibodies post Resources on Addgene.org Browse Addgene’s Antibodies plasmid collection...
  20. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

    Type
    Blog Post
    Published
    April 6, 2021, 12:30 p.m.
    ...Extracts. Human Gene Therapy 31:664–678 . https://doi.org/10.1089/hum.2019.277 Joung J, Konermann S, Gootenberg...activation screening. Nat Protoc 12:828–863 . https://doi.org/10.1038/nprot.2017.016...
  21. Hot Plasmids and Viral Preps - January 2021

    Type
    Blog Post
    Published
    Jan. 5, 2021, 2:15 p.m.
    ...Sheets, MB, et al., ACS Synth Biol. 2020. https://pubs.acs.org/doi/10.1021/acssynbio.9b00395 O’Brian, SP, et...Lee, J. et al., Science Advances 2020. https://doi.org/10.1126/sciadv.abb7438 The CRISPR corner By Jennifer...
Showing: 451 - 480 of 814 results