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  1. Troubleshooting Your Plasmid Cloning Experiment

    Type
    Blog Post
    Published
    Sept. 24, 2019, 12:55 p.m.
    ...want to amplify sequences from a large genome organisms like mouse or human. There are several online ...about different plasmid elements Resources on Addgene.org Find protocols for plasmid cloning Read our ...

  2. Plasmids 101: Cre-lox

    Type
    Blog Post
    Published
    Jan. 13, 2015, 3:47 p.m.
    ...including activating an oncogene only in a particular organ, or bypassing embryonic lethality. To facilitate... K. 1993. PubMed PMID: 8513499. Resources on Addgene.org Find Cre plasmids Find loxP plasmids  ...

  4. Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

    Type
    Blog Post
    Published
    Aug. 24, 2021, 1:15 p.m.
    .... Mol. Life Sci. 76, 4689–4704 (2019). https://doi.org/10.1007/s00018-019-03239-6 Additional resources...in our Intro to Antibodies post Resources on Addgene.org Browse Addgene’s Antibodies plasmid collection...

  5. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

    Type
    Blog Post
    Published
    April 6, 2021, 12:30 p.m.
    ...Extracts. Human Gene Therapy 31:664–678 . https://doi.org/10.1089/hum.2019.277 Joung J, Konermann S, Gootenberg...activation screening. Nat Protoc 12:828–863 . https://doi.org/10.1038/nprot.2017.016...

  6. Hot Plasmids and Viral Preps - January 2021

    Type
    Blog Post
    Published
    Jan. 5, 2021, 2:15 p.m.
    ...Sheets, MB, et al., ACS Synth Biol. 2020. https://pubs.acs.org/doi/10.1021/acssynbio.9b00395 O’Brian, SP, et...Lee, J. et al., Science Advances 2020. https://doi.org/10.1126/sciadv.abb7438 The CRISPR corner By Jennifer...

  8. Rabies and Neuronal Tracing

    Type
    Blog Post
    Published
    May 29, 2018, 1:51 p.m.
    ...connected to the neck. Shortly after, we learned about organs and the jobs they perform. This became foundational...Gene Expression with FLEx Vectors Resources on Addgene.org AAV Vector for TVA Expression AAV for Rabies...

  9. Adeno Associated Virus (AAV) for Cell and Gene Therapy

    Type
    Blog Post
    Published
    Nov. 7, 2017, 1:59 p.m.
    ...investigated including lentiviruses, adenovirus, and inorganic delivery tools such as nanoparticles. These technologies...Addgene AAV Quality Control Process Resources on Addgene.org Find ready-to-use AAV in Addgene's Viral Service...

  10. Science communication: 9 Strategies to Get Your Foot in the Door

    Type
    Blog Post
    Published
    Sept. 27, 2017, 1:23 p.m.
    ...work or volunteer for a science communication organisation like Building with Biology. Local environmental...Science Communication Additional Resources on Addgene.org Find Job Openings at Addgene ...

  11. 6 Tips for Grant Writing

    Type
    Blog Post
    Published
    Oct. 18, 2016, 2:30 p.m.
    ...used to conversing with other scientists that we forget that some of the terminology we use routinely isn...applications for funding from charities, commercial organizations, or universities may have someone senior from...

  12. Tips for Technical Support Calls

    Type
    Blog Post
    Published
    Sept. 20, 2016, 2:39 p.m.
    ... the company’s FAQs? A lot of companies and organizations selling biological reagents have a ton of data...with the Addgene Deposit Process Resources on Addgene.org Check Out Our Frequently Asked Questions Pages...

  13. A Look at Addgene's QC Process

    Type
    Blog Post
    Published
    May 6, 2025, 1:15 p.m.
    ...fully sequenced by Addgene’s QC team. Email help@addgene.org to get started! Addgene’s QC process Addgene ...Addgene Bioinformatics at Addgene Resources on Addgene.org Educational Resources Sequence Analysis of a...

  14. Antibodies 101: Stripping and Reprobing Western Blots

    Type
    Blog Post
    Published
    Aug. 1, 2024, 1:15 p.m.
    ...antibody you used to assess the stripping, and don’t forget to re-block your membrane! Depending on the system...out other Antibody 101 articles Resources on addgene.org Western Blot Protocol Western Blot Protocol Video...

  15. Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux

    Type
    Blog Post
    Published
    Sept. 13, 2016, 2:30 p.m.
    ...Advocating for Science Symposium and Workshop, organized by Future of Research, Academics for the Future... More information can be found at http://futureofresearch.org/advocating-for-science-boston-2016/. Sign...

  16. Adapting Toehold Switches to Detect Zika Virus

    Type
    Blog Post
    Published
    Aug. 30, 2016, 2:30 p.m.
    ...Guillain-Barré syndrome. In response, the World Health Organization has declared a global health emergency and called...Used to Study Dangerous Viruses Resources on Addgene.org Check out All the Plasmids from the Collin's...

  17. 5 Tips for Troubleshooting Viral Transductions

    Type
    Blog Post
    Published
    Aug. 11, 2016, 2:23 p.m.
    ...PMID: 12036457. 6. Davis, Howard E., Jeffrey R. Morgan, and Martin L. Yarmush. "Polybrene increases retrovirus...Lentiviral Vectors Additional Resources on the Addgene.org Check out Our Viral Vector Guide Pages Find ...

  18. Popular Retroviral Vectors and Their Uses in Scientific Research

    Type
    Blog Post
    Published
    July 24, 2018, 12:51 p.m.
    ...our CRISPR reagents. Please check www.genome-engineering.org for the most up-to-date information. Check ...guide to all things lentivirus Resources on Addgene.org Gamma-retrovirus Guide Lentivirus Guide ...

  19. Corralling and Cornering the Goldilocks Antibody

    Type
    Blog Post
    Published
    Aug. 9, 2022, 1:15 p.m.
    ...in research. Nature 518, 27–29 (2015). https://doi.org/10.1038/518027a Freedman LP, Cockburn IM, Simcoe...Preclinical Research. PLoS Biol 13(6): e1002165. https://doi.org/10.1371/journal.pbio.1002165 More resources on ...

  21. Mouse Modeling, Part 1: Genetically Engineered Mice

    Type
    Blog Post
    Published
    July 11, 2019, 1:26 p.m.
    ...Mice are a common model organism used to understand mammalian traits and genetically engineered mouse... genome engineering blog posts Resources on Addgene.org Find more about Cre-lox Read our Genome Engineering...

  22. PCR Applications for Copy Number Variation Assays

    Type
    Blog Post
    Published
    April 26, 2022, 1:15 p.m.
    ...Reaction Overview and Applications Resources on Addgene.org Protocol: Polymerase Chain Reaction (PCR) Protocol...and FCGR3B. PloS one, 10(1), e0116791. https://doi.org/10.1371/journal.pone.0116791 Providenti MA, O'Brien...

  23. PhD Applications After COVID

    Type
    Blog Post
    Published
    Jan. 5, 2023, 2:15 p.m.
    ...more comfortable and help you to keep yourself organized. By writing down your key points and keeping them...  Ashley Bastin is a first year PhD student at George Washington University whose research focuses on...

  24. Plasmids 101: Protein Expression

    Type
    Blog Post
    Published
    June 7, 2018, 1:17 p.m.
    ...frequently used species due to its use as a model organism in genetics and biochemistry. When using S. cerevisiae...promoters Check out protein tags Resources on Addgene.org Bacterial expression systems Search for plasmids...

  25. Transferable Skills Guide: Managing a Team

    Type
    Blog Post
    Published
    Jan. 30, 2018, 2:42 p.m.
    ...Positive feedback is equally as important so don’t forget to recognize someone’s hard work and reward a job...Industry and Success in Management Resources at Addgene.org Addgene's Management Team ...

  26. Pooled Library Amplifications

    Type
    Blog Post
    Published
    May 30, 2024, 1:30 p.m.
    ...protocol, feel free to contact us at deposit@addgene.org. Happy amplifying! More resources on the Addgene...Quality Control for Pooled Libraries Resources on Addgene.org Addgene's Guide to Plasmid Pooled Libraries  ...

  27. Addgene's Cancer Collection Pages Connect the Research Community

    Type
    Blog Post
    Published
    Sept. 6, 2016, 2:30 p.m.
    ...proliferation. Hence it is imperative to the health of an organism that both classes of genes function normally to...Used in Cancer Lineage Tracing Resources on Addgene.org Visit our Cancer Collection Pages Deposit Your...

  28. Anatomy of a Plasmid Page at Addgene

    Type
    Blog Post
    Published
    Feb. 4, 2016, 3:30 p.m.
    ... please don't hesitate to contact us at help@addgene.org Resources on the Addgene Blog Read about Our... Do Quality Control at Addgene Resources on Addgene.org Join the Addgene Community Learn the Benefits...

  30. Uncovering Ancestral Bias in CRISPR Research

    Type
    Blog Post
    Published
    Nov. 29, 2022, 2:15 p.m.
    ...Boehm. bioRxiv 2022.11.18.517155; doi: https://doi.org/10.1101/2022.11.18.517155   Resources on ancestral...diversity. Nature 604, 437–446 (2022). https://doi.org/10.1038/s41586-022-04601-8 Lewis, Anna CF, Santiago...
Showing: 451 - 480 of 795 results