We narrowed to 796 results for: arc

1. Plasmids 101: Terminators and PolyA signals

   Type

   Blog Post

   Published

   March 31, 2016, 2:30 p.m.

   ...expression in a context-dependent manner. Nucleic Acids Research. 2000. PubMed PMID:10648781. PubMed Central PMCID...

2. Tips for Titering Your Lentiviral Preps

   Type

   Blog Post

   Published

   March 15, 2016, 2:30 p.m.

   ...resistance-genes, or the transgene itself. Many researchers prefer to design primers that target the common...

3. 7 Simple Strategies to Resolve Conflicts with Difficult Supervisors

   Type

   Blog Post

   Published

   Jan. 21, 2015, 1:30 p.m.

   ... to Expedite Your Experiments and Achieve Your Research Goals    ...

4. Fluorescent Tagging of Endogenous Genes with SapTrap

   Type

   Blog Post

   Published

   Oct. 12, 2017, 2:26 p.m.

   ...with either blasticidin or puromycin selection. Researchers at the Allen Institute for Cell Science recently...

5. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

   Type

   Blog Post

   Published

   April 4, 2019, 12:53 p.m.

   ...knockdown, however, keeps bacteria viable and allows researchers to study if that gene is a target of an antibiotic...

6. Tips for a 1st time AAV user (by a Rookie AAV user)

   Type

   Blog Post

   Published

   Oct. 23, 2018, 11:49 a.m.

   ...out Addgene's virus protocols Find AAV for your research ...

7. Cloning Mammalian Cells with the Agarose Method

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   Blog Post

   Published

   Sept. 7, 2017, 12:17 p.m.

   ... biological screens. For example, for cancer researchers, if growth properties in agarose are of interest...

8. Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles

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   Blog Post

   Published

   June 22, 2017, 2:30 p.m.

   ...function of a protein or its role in a disease, researchers often isolate proteins of interest and examine...

9. Polymerase Chain Reaction Overview and Applications

   Type

   Blog Post

   Published

   Aug. 10, 2021, 1:15 p.m.

   ... was first identified in the late 1960s during research at hot springs in Yellowstone National Park.  ...

10. Viral Vectors 101: Voltage Indicators

    Type

    Blog Post

    Published

    Jan. 30, 2024, 2:15 p.m.

    ...ability to monitor their signals – electricity! The research tools for studying voltage within a cell are genetically...

11. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

    Type

    Blog Post

    Published

    Dec. 10, 2020, 1 p.m.

    ...using adenoviral vectors with your CRISPR/Cas9 research! To find more information about the adenoviral...

12. Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards

    Type

    Blog Post

    Published

    May 22, 2015, 4:58 p.m.

    ...exclusively because historical studies of pathogen research have conclusively shown that barrier protocols...

13. Plasmids 101: Secondary Nanobody Toolbox

    Type

    Blog Post

    Published

    Feb. 27, 2018, 2:04 p.m.

    ...you ready to start using nanobodies for your own research? Great! Pleiner et al. have done the hard work...

14. DNA Purification Without a Kit

    Type

    Blog Post

    Published

    Feb. 11, 2020, 2:15 p.m.

    ...number of spin columns you need to complete your research. Do you know of other nucleic acid purification...

15. A Guide to Starting Your Own Journal Club

    Type

    Blog Post

    Published

    Feb. 6, 2020, 3 p.m.

    ... of learning about new cutting edge science, researchers of all varieties must stay current with literature...

16. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

    Type

    Blog Post

    Published

    Aug. 30, 2022, 1:15 p.m.

    ...your target epitope(s). To achieve these goals, researchers commonly use one of two types of fixatives - ...

17. Antibodies 101: Producing Recombinant Antibodies

    Type

    Blog Post

    Published

    Aug. 13, 2024, 1:15 p.m.

    .... Recombinant Antibodies in Basic Neuroscience Research. Curr Protoc Neurosci. 2020 Dec;94(1):e106. doi...

18. Plasmids 101: Choosing an Antibiotic Resistance Gene

    Type

    Blog Post

    Published

    Oct. 10, 2023, 1 p.m.

    ...universally compatible with all bacteria used in research, it isn’t a widely popular selection agent. Pros...

19. Tips for Screening with Yeast Two Hybrid Systems

    Type

    Blog Post

    Published

    Oct. 22, 2015, 2:30 p.m.

    ...that retain their individual activities (1). Researchers also discovered the modular format of some transcriptional...

20. Cpf1: A New Tool for CRISPR Genome Editing

    Type

    Blog Post

    Published

    Oct. 14, 2015, 2:30 p.m.

    ...location and GC-content. After sequencing and searching for cellular RNAs important for Cpf1 function,...

21. Plasmids 101: Protein tags

    Type

    Blog Post

    Published

    Dec. 11, 2014, 4:26 p.m.

    ...decades of trying to address these challenges, researchers have amassed a considerable molecular tool box...

22. Sonic Hedgehog? Sleeping Beauty? Learn About the Genes Behind Addgene’s Conference Room Names

    Type

    Blog Post

    Published

    April 2, 2019, 1:44 p.m.

    ...Resources on Addgene.org Find plasmids for your research Deposit plasmids at Addgene  ...

23. CRISPR-mediated Plant Base Editors

    Type

    Blog Post

    Published

    Jan. 3, 2019, 1:35 p.m.

    ... is an Ag-biotechnologist in the National Rice Research Institute, Cuttack, Odisha, India. Currently Kutubuddin...

24. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type

    Blog Post

    Published

    Sept. 16, 2020, 1 p.m.

    ... efficiency than its wild-type counterpart.  In search of a smaller, but equally potent Cas9 protein, ...

25. Finding nucleic acids with SHERLOCK and DETECTR

    Type

    Blog Post

    Published

    April 16, 2020, 1 p.m.

    ...Durbin AF, Kellner MJ, Tan AL, Paul LM, Parham LA, Garcia KF, Barnes KG, Chak B, Mondini A, Nogueira ML, ...

26. Plasmids 101: Plasmid Incompatibility

    Type

    Blog Post

    Published

    March 31, 2020, 1:15 p.m.

    ...et al., 2015). Now, bioinformatics tools help researchers determine how compatible their plasmids will ...

27. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type

    Blog Post

    Published

    Sept. 21, 2017, 2:07 p.m.

    ...genes that fell into 3 categories based on the architecture of the CRISPR protein in question: C2c1, C2c2...

28. Fluorescent Proteins 101: Aptamer Fluorophores

    Type

    Blog Post

    Published

    April 11, 2017, 2:30 p.m.

    ...or as fluorophores in several different ways. Researchers have used oligonucleotides in fluorescent visualization...

29. Plasmids 101: Gateway Cloning

    Type

    Blog Post

    Published

    Jan. 12, 2017, 3:30 p.m.

    ... genes, including Hs.KRAS4a. Use our website to search for your favorite gene! STEP 2: Generate an Expression...

30. Plasmids 101: Stringent Regulation of Replication

    Type

    Blog Post

    Published

    June 20, 2024, 1:15 p.m.

    ...initiation of chromosome replication. Nucleic Acids Research, 46(12), 6140–6151. https://doi.org/10.1093/nar...

31. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

    Type

    Blog Post

    Published

    Oct. 13, 2022, 1:45 p.m.

    ...modifications. Cas9 has been modified to enable researchers to knock out, knock in, base edit, activate, ...

32. Getting the Most from Your Lentiviral Transduction

    Type

    Blog Post

    Published

    April 7, 2016, 2:30 p.m.

    ...Resources on Addgene.org Find Viral Vectors for Your Research Brush Up on All Things Lentivirus with our Lentiviral...

33. The Fluorescent Vegetables in Aptamer Soup

    Type

    Blog Post

    Published

    Jan. 29, 2019, 2:27 p.m.

    ... instead activates Spinach fluorescence. In the search for something more stable for quantitative imaging...

34. Plasmids 101: Biotinylation

    Type

    Blog Post

    Published

    Nov. 15, 2018, 1:50 p.m.

    ...ssDNA or RNA–protein interactions.” Nucleic Acids Research, 23.18 (1995): 3792–3793. PubMed PMID: 7479014...

35. Hot Plasmids: Summer 2024

    Type

    Blog Post

    Published

    Aug. 27, 2024, 1:15 p.m.

    ... Structural determination on magnetic beads for scarce macromolecules in heterogeneous samples. bioRxiv...

36. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type

    Blog Post

    Published

    Sept. 28, 2023, 1:15 p.m.

    ...more to optogenetics than neuronal activity. Researchers have designed a variety of photoswitchable elements...

37. Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods

    Type

    Blog Post

    Published

    March 1, 2016, 3:30 p.m.

    ... with Linkers (MODAL) strategy." Nucleic acids research 42.1 (2014): e7-e7. PubMed PMID: 24153110. PubMed...

38. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

    Type

    Blog Post

    Published

    April 24, 2015, 2:39 p.m.

    ...systems. Interested in applying Brainbow to your research? Check out Brainbow plasmids available from Addgene...

39. Antibodies 101: The Basics of Western Blotting

    Type

    Blog Post

    Published

    March 8, 2021, 2:41 p.m.

    ... that you can make on your own.  To narrow your search, first pay attention to the validated applications...

40. Viral Vectors 101: AAV Serotypes and Tissue Tropism

    Type

    Blog Post

    Published

    Jan. 16, 2025, 3:42 p.m.

    ...numbered AAV1, AAV2, and so on. In addition, researchers have created many engineered serotypes by mixing...

41. Technique: Probe Phage Genomes for Host Binding Proteins

    Type

    Blog Post

    Published

    Sept. 29, 2016, 2:30 p.m.

    ...the (all-too-common) event that homology-based searches return no candidates. Our strategy: Express random...

42. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

    Type

    Blog Post

    Published

    March 7, 2017, 3:30 p.m.

    ...pretty amazing. Despite the shifting landscape of research opportunities, it’s an exciting time to be in ...

43. Lambda Red: A Homologous Recombination-based Technique for Genetic Engineering

    Type

    Blog Post

    Published

    Dec. 15, 2016, 3:57 p.m.

    ...blogger, Beth Kenkel Beth Kenkel is currently a research scientist in the Department of Laboratory Medicine...

44. Antibodies 101: Designing Your First Flow Panel

    Type

    Blog Post

    Published

    May 28, 2024, 1:45 p.m.

    ...design your first flow panels! Paul Heisig is a Research Associate in the lab of Arlene Sharpe at Harvard...

45. Which Fluorescence Microscopy Techniques is Best for Me?

    Type

    Blog Post

    Published

    Oct. 10, 2017, 1:57 p.m.

    ...of tissue clearing techniques (9) now allows researchers to image tissues that are over a cm3 in size....

46. Technical Design of a Western Blot

    Type

    Blog Post

    Published

    Aug. 22, 2024, 1:15 p.m.

    ...modifications, we recommend doing a little more research into gel chemistry and/or specialty gel options...