We narrowed to 329 results for: AGA

1. Tips for Getting a Faculty Position

   Type

   Blog Post

   Published

   July 20, 2023, 1:15 p.m.

   ...create a new field, impact disease treatment, etc., again in clear terms. "Implications for human disease"...

2. Filming Science Videos in the Age of Social Distancing

   Type

   Blog Post

   Published

   May 20, 2021, 1:15 p.m.

   ...what works and what doesn’t, and trying all over again. Here’s that process!   Figure 1: The Design-Build-Test-Learn...

3. Site Directed Mutagenesis by PCR

   Type

   Blog Post

   Published

   Aug. 2, 2016, 2:30 p.m.

   ...fragments should be separated on a high percentage agarose gel (~3%). Note that primer duplication will evade...

4. Evolution of Lab Techniques

   Type

   Blog Post

   Published

   June 21, 2016, 2:30 p.m.

   ...restriction enzymes and then running the products on an agarose gel allows a researcher to properly orient specific...

5. Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility

   Type

   Blog Post

   Published

   May 18, 2018, 7:32 p.m.

   ...directly by creating well characterised antibodies against all human extracellular proteins with community-based...

6. CRISPR 101: Epigenetics and Editing the Epigenome

   Type

   Blog Post

   Published

   June 24, 2020, 5:45 p.m.

   ...experimental period, as this mark was faithfully propagated in culture and in vivo. In certain cases, epigenetic...

7. Hot Plasmids: Spring 2025

   Type

   Blog Post

   Published

   June 3, 2025, 1:15 p.m.

   ... set of highly-specific recombinant antibodies against three of the six human glypican proteins (GPC1,...

8. Viral Vectors 101: AAV Serotypes and Tissue Tropism

   Type

   Blog Post

   Published

   Jan. 16, 2025, 3:42 p.m.

   ...Diep, J., Ishikawa, Y., Jae, L. T., Wosen, J. E., Nagamine, C. M., Chapman, M. S., & Carette, J. E. (2016...

9. Antibodies 101: The Basics of Western Blotting

   Type

   Blog Post

   Published

   March 8, 2021, 2:41 p.m.

   ...blocked, it is stained with a primary antibody against your protein of interest. But which antibody should...

10. Finding Your Perfect Job After University

    Type

    Blog Post

    Published

    Jan. 12, 2016, 3:30 p.m.

    ...Working in industry: virology On completing my MSc, I again had to look at what type of work I would like to...

11. When Fidelity Matters: A frank discussion about ligase fidelity

    Type

    Blog Post

    Published

    March 6, 2018, 1:49 p.m.

    ...DNA ligase, are naturally able to discriminate against ligating substrates containing base pair mismatches...

12. Quick Guide to All Things Lentivirus

    Type

    Blog Post

    Published

    March 21, 2017, 2:30 p.m.

    ...multiplicity of infection (MOI=ratio of virus to cells). Again, my tip here is to test several protocols as the...

13. Lambda Red: A Homologous Recombination-based Technique for Genetic Engineering

    Type

    Blog Post

    Published

    Dec. 15, 2016, 3:57 p.m.

    ...temperature allows the repressor to re-nature and again repress expression of exo, beta and gam.  A major...

14. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

    Type

    Blog Post

    Published

    Nov. 2, 2021, 1:15 p.m.

    ...j.ymthe.2004.05.024 Dudek AM, Pillay S, Puschnik AS, Nagamine CM, Cheng F, Qiu J, Carette JE, Vandenberghe LH...

15. Transferable Skills Guide: Teamwork

    Type

    Blog Post

    Published

    Nov. 28, 2017, 2 p.m.

    ...matter whether that other person really is acting against the team’s interests or not; once that seed of ...

16. Sequencing Options for CRISPR Genotyping

    Type

    Blog Post

    Published

    Oct. 4, 2016, 2:30 p.m.

    ...equitable society. Read more at https://magazine.scienceforthepeople.org/.     References 1. Fu, Yanfang, et...

17. Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein

    Type

    Blog Post

    Published

    June 28, 2018, 3:01 p.m.

    ...complexes - from the cell-lysate using antibodies against the target protein. The captured protein complexes...

18. Custom CRISPR Screens & the Green Listed Software

    Type

    Blog Post

    Published

    July 11, 2017, 2:30 p.m.

    ...Another interesting approach is to design screens against known druggable targets, preferentially those for...

19. Antibodies 101: Designing Your First Flow Panel

    Type

    Blog Post

    Published

    May 28, 2024, 1:45 p.m.

    ...briefly go through each section of this article again. In the beginning, we talked about the relationship...

20. A Primer on Optogenetics: Introduction and Opsin Delivery

    Type

    Blog Post

    Published

    Sept. 10, 2015, 2:30 p.m.

    ...for sufficient opsin to reach the axon terminal. Again, this must be empirically determined in your own...