We narrowed to 582 results for: RON

...number, but it was attained without any of the error-prone repair that normally accompanies Cas9 cutting. We... holds some kind of fitness advantage but error-prone repair of the gene would be disastrous (i.e. if ...

...effective delegation skills: The team will be stronger and less dependent on the manager to succeed. ... your team and take responsibility if things go wrong. Here are some important criteria for delegating...

...biosensing technique can be applied to an important neuronal signalling pathway - if you’ve got a useful tool...genes to specific cell types in the brain, mapping neuronal networks, and selectively activating signaling...

...go of the DNA. ABEs are somewhat slow and need a strong Cas9 domain to “hold on” long enough to the DNA...Richter MF, Zhao KT, Eton E, Lapinaite A, Newby GA, Thuronyi BW, Wilson C, Koblan LW, Zeng J, Bauer DE, Doudna...

...high GC content and hairpin structures, ITRs are prone to replication errors and disruptive mutations and...improvements unrelated to capsid protein modification. Front Med, 10, 1106085. https://doi.org/10.3389/fmed.2023.1106085...

...immunogenicity, which means they do not stimulate a strong immune response when introduced into an organism...Harbor, NY, USA: 1997. PubMed PMID: 21433340. 4. Cronin J, et al. “Altering the tropism of lentiviral vectors...

... use amplicons in ITR as we have found this to strongly bias titer. We are also adopting ddPCR, which ...microglia? I have been using the AAV.PHP.eb for neuron, oligodendrocyte, and astrocyte specificity and...

... S, Bladen CL, Jones B, Aldred SF, Bestagno M, Burrone O, Maynard J, Ferrara F, Trimmer JS, Görnemann ...doi.org/10.1093/ilar.46.3.258  Mould AP, Askari JA, Byron A, Takada Y, Jowitt TA, Humphries MJ (2016) Ligand-induced...

...Transformation is the uptake of genetic material from the environment by bacterial cells. In nature, this genetic material...plasmid DNA or fragmented DNA released into the environment. Various factors promote natural transformation...

...improved RNAi vector that leads to significantly stronger phenotypes than standard RNAi protocols without...Recently, the Sidhu group at the University of Toronto expanded the specificity landscape of the SH3 proteins...

...make a knockout in your gene of interest, as it is prone to generating indel errors. Indel errors generated...Non-Homologous End-Joining Really an Inherently Error-Prone Process? PLoS Genet 10:e1004086 . https://doi.org...

...other than to sustain those techniques. We feel strongly that the scientific and the agricultural community...themselves if they want this beautiful flower in their front yard or not. By developing plant aesthetics with...

...and a specialist. Notably, the team must have an upfront conversation about its goals and vision for the...us about the biotech startup process and work environment! Interested in Synthetic Biology? Read On! Synthetic...

...disease in question – cardiomyocytes, adipocytes or neurons, for instance – to see how those mutations influence...TALENs may be cleaner than CRISPR/Cas9. Reference: Quirong Ding et al. "A TALEN genome-editing system for ...

... means that F(ab’)2 fragments may have overall stronger interaction with their antigens than a Fab or ... Ashley Waldron...

... psoriasis Generalized pustular psoriasis is a chronic autoinflammatory skin disease estimated to affect...genetically-encoded biosensors and iPSC-derived cortical neurons from patients with GNB1 mutations, the lab hopes...

...degraded. To effectively isolate intact RNA, a fast, strong protein denaturant would be required- something...late 1970’s, Chirgwin and colleagues showed that a strong protein denaturant, guanidinium thiocyanate, did...

...particularly helpful when wanting to target specific neuron subtypes, since AAVs tend to be very neuro-permissive...Postdoctoral Fellow in Connie Cepko’s Lab investigating neuronal circuits of the retina using viral tools.   References...

...Transcription, translation, and prone to degradation – those are the words that describe RNA! Double ...telomeres and centromeres. These genomic regions are prone to persistent R-loop formation, presumably due to...

...more useful! From FPs optimized for oxidizing environments to photoconvertible variants, it seems like ... cells infected with AAV-Cre-DOG retain normal neuronal function, this system may make optogenetics in...