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We narrowed to 749 results for: SOM

Showing: 301 - 320 of 749 results

  1. Addgene Protocols Go Viral: AAV Purification and Titration

    Type
    Blog Post
    Published
    June 13, 2019, 12:19 p.m.
    ...the loading and unloading of the column and give some tips for performing this purification.  Check out...titration by qPCR using SYBR green technology Here’s some advice on how to successfully set up your qPCR to... new to tissue culture, check out this video for some tips and best practices for getting started with...

  2. Depositor Week 2020 is Happening Now. Join us!

    Type
    Blog Post
    Published
    Nov. 16, 2020, 2:15 p.m.
    ...these plasmid deposits came in this year, there were some plasmids deposited as early as 2006 that have helped...your science along? We’ll also take a look back on some fascinating Addgene history that you might not have... into 2021. Check out the infographic below for some Addgene depositor fun facts for a quick way to grasp...

  3. Addgene's Website Update

    Type
    Blog Post
    Published
    April 8, 2024, 2:15 p.m.
    ...content teams have made some changes to the look and feel of addgene.org. Some you’ll notice right away...Starting today, you may notice some changes to our website! Part of sharing science and making access...

  4. Q&A With Julie Cicalese, the Newest Member of the Addgene Board of Directors

    Type
    Blog Post
    Published
    March 17, 2021, 2:40 p.m.
    ...colleague. She mentioned Addgene was looking for someone with HR expertise to join their newly formed compensation... and also serves as an objective body to oversee some of Addgene’s compensation decisions. It was a great... enough people will be vaccinated and we’ll have some herd immunity, which raises questions like “How ...

  5. Your Top Requested Plasmid in 2016!

    Type
    Blog Post
    Published
    Jan. 11, 2017, 8:47 p.m.
    ...transfection. The 2A "self cleaving" peptide causes the ribosome to translate Cas9-2A and the Puromycin resistance...the name? Not included in this plasmid's name are some additional features. The first and most imporant...Learn about Using CRISPR to Generate Knockouts Read Some Common Ways to Deliver CRISPR Plasmids to Mammalian...

  7. From our Table to Yours: An Inside Look at Lunch at Addgene

    Type
    Blog Post
    Published
    Nov. 27, 2013, 2:54 p.m.
    ...an indication of the stiff competition or says something about my cooking.   This is a creative (and delicious...competitions in the future, and hopefully include some of our favorite winning recipes. Hack Day lunches...Director, Joanne Kamens, responded by starting something we call “Random Lunches.” Once or twice a year...

  8. A new platform for fast functional characterization of potentially cancerous mutations

    Type
    Blog Post
    Published
    May 7, 2019, 12:39 p.m.
    ...Plasmid Collection for “Functional Annotation of Somatic Mutations in Cancer.” Of all the mutations and ...how a given gene is involved in a complex pathway. Some of the most heavily-studied genes in cancer research...Kwok-Shing, et al. "Systematic functional annotation of somatic mutations in cancer." Cancer Cell 33.3 (2018): ...

  10. Science as Art

    Type
    Blog Post
    Published
    March 2, 2015, 3:13 p.m.
    ... of art to me… The problem is that scientists sometimes forget how to communicate about their work with...and I believe it could have made the difference in some areas of conservation.” Appreciating science as ...focused his x-ray machine not on teeth, but on blossoms, thus revealing the startling inner beauty of ...

  11. Inside Addgene's 10 Year Anniversary Gala

    Type
    Blog Post
    Published
    July 3, 2014, 3:48 p.m.
    ... was set aside for the event. As guests arrived, some gravitated towards the sushi and drinks, while others...Addgene family. It was great to see the faces of some of our depositing scientists who weren’t able to... roam the museum. I even posed for pictures with some Addgenies. As the gala came to an end, it was clear...

  12. Working 9-5: an Addgene Co-op

    Type
    Blog Post
    Published
    June 2, 2022, 2:15 p.m.
    ...was useful to me as some days it was not as easy to commute as others. I know some fear that remote work... Working a 9-5 As it turned out, Addgene uses something called “core hours”. Core hours consist of the...

  13. A Better Way to Get Customer Support: The Help Center

    Type
    Blog Post
    Published
    Dec. 10, 2015, 3:30 p.m.
    ...support team available whenever you need them, but sometimes that’s not enough. With your busy schedule, you...prefer to troubleshoot yourself without contacting someone else and waiting for an answer. Luckily, there’...easier than ever to get the support you need. If for some reason you can’t find the answer that you’re looking...

  14. 6 Steps to Submitting a Resume That Gets Seen

    Type
    Blog Post
    Published
    July 9, 2015, 2:30 p.m.
    ...excitement, in a position. Everyone prefers to hire someone that will be excited to be there. If you are appying..., you will need to be more directed and target someone in a specific department and/or location. The more...asking for help. The worst thing that can happen is someone will say no, but if you don't ask, you will never...

  15. Plasmids 101: Positive and Negative Selection for Plasmid Cloning

    Type
    Blog Post
    Published
    Aug. 22, 2019, 12:43 p.m.
    ...successfully takes up plasmid DNA. Among those that do, some now have plasmids that contain your gene of interest...selecting for the loss of a gene product - usually something toxic. This gene is found on the original plasmid...alleviates its toxic effect. Negative selection is sometimes called counterselection.  Toxin-antitoxin systems...

  17. Antibodies 101: Affinity Tags

    Type
    Blog Post
    Published
    Sept. 19, 2023, 1 p.m.
    ...and everything in between – now all you need are some of your favorite proteins purified to carry out ...used for both direct and indirect visualization. Some of these tags can also be used for protein purification...purification, just as affinity tags can sometimes be repurposed for visualization. However, due to the nature...

  18. Cellosaurus: A Cell Line Information Database

    Type
    Blog Post
    Published
    Feb. 21, 2023, 2:15 p.m.
    ... labor-intensive project; scripts can be used in some cases, but much of this work has required manually...the graduate student down the hall who got it from some technician downstairs who got it from who knows ...submissions from scientists all over globe, with some surprising, and important, findings being shared...

  19. An Addgene Summer Internship

    Type
    Blog Post
    Published
    Aug. 8, 2024, 7:22 p.m.
    ...impression I got from the La Vida Scholars tour. After some time had passed, I reached out to Amy with exciting...the Summer Intern Poster Presentation.  As someone who had limited experience when it came to lab ...full potential as a scientist. But I expected something wrong. My capstone project was based on plasmid...

  20. Year of the Camelid: Antibody Style

    Type
    Blog Post
    Published
    May 9, 2024, 1:15 p.m.
    ...Camelid family, I wanted to take a moment to revisit some of the ways these animals have impacted humans through...and affinity reagents. But for a quick refresher, some of the advantages of sdAbs over conventional antibodies...libraries to generate new sdAbs. There has also been some interesting work done developing transgenic mice...
Showing: 301 - 320 of 749 results