We narrowed to 363 results for: his

1. CRISPR 101: Cas9 vs. The Other Cas(s)

   Type

   Blog Post

   Published

   Dec. 8, 2022, 2:15 p.m.

   ...to detect variants such as single nucleotide polymorphisms. Fast facts – PAM requirement: none. Best ...

2. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

   Type

   Blog Post

   Published

   Dec. 8, 2016, 3:30 p.m.

   ...ccdB accomplish this? Let’s start with a brief history of the gene and how molecular biologists have harnessed...

3. Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins

   Type

   Blog Post

   Published

   March 19, 2019, 1:31 p.m.

   ...coordinate emotions, thoughts, and behavior. Historically, scientists study the electrical activity in...

4. Reproducibility for Everyone: Lessons from an Open Science Collaboration

   Type

   Blog Post

   Published

   Feb. 28, 2019, 1:34 p.m.

   ... Schwessinger for helpful conversations on the history of the group, lessons learned, and to Sonali Roy...

5. Antibodies 101: Selecting the Right Antibody

   Type

   Blog Post

   Published

   March 15, 2022, 1:15 p.m.

   ...be variations within an application. Take immunohistochemistry (IHC), for instance. While an antibody may...

6. Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP

   Type

   Blog Post

   Published

   Nov. 24, 2015, 3:30 p.m.

   ...repurpose popular GFP reporter lines for more sophisticated experimental manipulations, saving the time ...

7. Career Coaching for Scientists: Why and Where Do I Find One?

   Type

   Blog Post

   Published

   Jan. 29, 2015, 1:25 p.m.

   ...has an outstanding blog and, while her PhD is in History, I think she would be a good match as a coach for...

8. Working with Nuclear Receptors

   Type

   Blog Post

   Published

   July 15, 2014, 4:03 p.m.

   ...other members of the complex, such as p300 and CBP histone acetyltransferases or CARM1 methyltransferase. ...

9. Plasmids 101: The Wide World of Natural Plasmids

   Type

   Blog Post

   Published

   Oct. 8, 2024, 1:15 p.m.

   ...cells survive their own secretions. Pro tip! Historically, colicin-producing plasmids have been referred...

10. Progress Towards a PAM-Free CRISPR

    Type

    Blog Post

    Published

    March 7, 2024, 2:39 p.m.

    ...Engineering a CRISPR Variant with PAM Flexibility History of CRISPR Cas – A Tale of Survival and Evolution...

11. Antibodies 101: Choosing the Right Isotype

    Type

    Blog Post

    Published

    April 6, 2023, 3 p.m.

    ...Antibody to Mouse Tissue Sections. In Journal of Histochemistry & Cytochemistry (Vol. 46, Issue 8, pp. ...

12. Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

    Type

    Blog Post

    Published

    Dec. 14, 2021, 2:15 p.m.

    ...enzymatic fusions are godsends. Tethering dCas9 to the histone acetyltransferase p300 or the DNA methyltransferase...

13. Engaging with science and society at pgEd

    Type

    Blog Post

    Published

    Sept. 20, 2018, 12:17 p.m.

    ...sticky ethical dilemmas to dark episodes in the history of eugenics and research misconduct. Past ethical...

14. Adenoviral Vector Production and Troubleshooting

    Type

    Blog Post

    Published

    June 14, 2018, 11:44 a.m.

    .... The viral genome DNA is further protected by histone-like proteins forming the nucleocapsid. B) Three...

15. CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!

    Type

    Blog Post

    Published

    Oct. 6, 2020, 1:15 p.m.

    ...-CBP In this method, dCas9 is fused to CBP, an histone acetyltransferase domain capable of rearranging...

16. Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors

    Type

    Blog Post

    Published

    Aug. 20, 2019, 12:31 p.m.

    ...science, dating back several hundred years, with historical ties to psychology and philosophy (the mind-body...

17. Science communication: 9 Strategies to Get Your Foot in the Door

    Type

    Blog Post

    Published

    Sept. 27, 2017, 1:23 p.m.

    ...or upload your data to Zooniverse. The Natural History Museum has downloadable resources to enable you...

18. Fluorescent Proteins 101: Introduction to FRET

    Type

    Blog Post

    Published

    June 27, 2017, 1:03 p.m.

    ...have enhanced the ability of labs to answer sophisticated questions about cellular processes. Currently...

19. Cpf1 Update: Comparison to Cas9 and NgAgo

    Type

    Blog Post

    Published

    July 14, 2016, 2:30 p.m.

    ... off-target editing rates, determined using sophisticated genome-wide analysis. At many of its computationally...

20. Viral Vectors 101: Voltage Indicators

    Type

    Blog Post

    Published

    Jan. 30, 2024, 2:15 p.m.

    ...a genetic component. The VSD components have historically come from a variety of sources – voltage-gated...