We narrowed to 365 results for: his

1. Antibodies 101: Conjugation

   Type

   Blog Post

   Published

   March 26, 2024, 1:15 p.m.

   ...typically used in microscopy-based assays like immunohistochemistry, though they are also a common choice for...

2. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

   Type

   Blog Post

   Published

   Dec. 8, 2016, 3:30 p.m.

   ...ccdB accomplish this? Let’s start with a brief history of the gene and how molecular biologists have harnessed...

3. CRISPR 101: Cas9 vs. The Other Cas(s)

   Type

   Blog Post

   Published

   Dec. 8, 2022, 2:15 p.m.

   ...to detect variants such as single nucleotide polymorphisms. Fast facts – PAM requirement: none. Best ...

4. Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP

   Type

   Blog Post

   Published

   Nov. 24, 2015, 3:30 p.m.

   ...repurpose popular GFP reporter lines for more sophisticated experimental manipulations, saving the time ...

5. Antibodies 101: Selecting the Right Antibody

   Type

   Blog Post

   Published

   March 15, 2022, 1:15 p.m.

   ...be variations within an application. Take immunohistochemistry (IHC), for instance. While an antibody may...

6. Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins

   Type

   Blog Post

   Published

   March 19, 2019, 1:31 p.m.

   ...coordinate emotions, thoughts, and behavior. Historically, scientists study the electrical activity in...

7. Reproducibility for Everyone: Lessons from an Open Science Collaboration

   Type

   Blog Post

   Published

   Feb. 28, 2019, 1:34 p.m.

   ... Schwessinger for helpful conversations on the history of the group, lessons learned, and to Sonali Roy...

8. Career Coaching for Scientists: Why and Where Do I Find One?

   Type

   Blog Post

   Published

   Jan. 29, 2015, 1:25 p.m.

   ...has an outstanding blog and, while her PhD is in History, I think she would be a good match as a coach for...

9. Working with Nuclear Receptors

   Type

   Blog Post

   Published

   July 15, 2014, 4:03 p.m.

   ...other members of the complex, such as p300 and CBP histone acetyltransferases or CARM1 methyltransferase. ...

10. Progress Towards a PAM-Free CRISPR

    Type

    Blog Post

    Published

    March 7, 2024, 2:39 p.m.

    ...Engineering a CRISPR Variant with PAM Flexibility History of CRISPR Cas – A Tale of Survival and Evolution...

11. Science communication: 9 Strategies to Get Your Foot in the Door

    Type

    Blog Post

    Published

    Sept. 27, 2017, 1:23 p.m.

    ...or upload your data to Zooniverse. The Natural History Museum has downloadable resources to enable you...

12. Fluorescent Proteins 101: Introduction to FRET

    Type

    Blog Post

    Published

    June 27, 2017, 1:03 p.m.

    ...have enhanced the ability of labs to answer sophisticated questions about cellular processes. Currently...

13. Engaging with science and society at pgEd

    Type

    Blog Post

    Published

    Sept. 20, 2018, 12:17 p.m.

    ...sticky ethical dilemmas to dark episodes in the history of eugenics and research misconduct. Past ethical...

14. Adenoviral Vector Production and Troubleshooting

    Type

    Blog Post

    Published

    June 14, 2018, 11:44 a.m.

    .... The viral genome DNA is further protected by histone-like proteins forming the nucleocapsid. B) Three...

15. Plasmids 101: The Wide World of Natural Plasmids

    Type

    Blog Post

    Published

    Oct. 8, 2024, 1:15 p.m.

    ...cells survive their own secretions. Pro tip! Historically, colicin-producing plasmids have been referred...

16. Antibodies 101: Choosing the Right Isotype

    Type

    Blog Post

    Published

    April 6, 2023, 3 p.m.

    ...Antibody to Mouse Tissue Sections. In Journal of Histochemistry & Cytochemistry (Vol. 46, Issue 8, pp. ...

17. Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors

    Type

    Blog Post

    Published

    Aug. 20, 2019, 12:31 p.m.

    ...science, dating back several hundred years, with historical ties to psychology and philosophy (the mind-body...

18. Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

    Type

    Blog Post

    Published

    Dec. 14, 2021, 2:15 p.m.

    ...enzymatic fusions are godsends. Tethering dCas9 to the histone acetyltransferase p300 or the DNA methyltransferase...

19. CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!

    Type

    Blog Post

    Published

    Oct. 6, 2020, 1:15 p.m.

    ...-CBP In this method, dCas9 is fused to CBP, an histone acetyltransferase domain capable of rearranging...

20. Cpf1 Update: Comparison to Cas9 and NgAgo

    Type

    Blog Post

    Published

    July 14, 2016, 2:30 p.m.

    ... off-target editing rates, determined using sophisticated genome-wide analysis. At many of its computationally...