We narrowed to 809 results for: org
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TypeBlog PostPublishedMay 17, 2022, 1:15 p.m....systemically transduce and then retransduce a model organism such as mice, e.g. first at 2 months, then at ...field has settled on ‘best practices’ for model organisms. Q11: How consistent is expression is when ...Tips for a first time AAV user Resources on Addgene.org Find Adenovirus Plasmids Adenovirus Guide Page...
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Expanding the Targeting Scope and Editing Efficiency of Adenine Base Editors
TypeBlog PostPublishedMarch 17, 2020, 1:32 p.m....without DNA cleavage. Nature 551:464–471 . https://doi.org/10.1038/nature24644 Richter MF, Zhao KT, Eton E...compatibility and activity. Nature Biotechnology. https://doi.org/10.1038/s41587-020-0453-z Additional resources ... in our CRISPR 101 blog series Resources on Addgene.org Find base editors for your research Read our... -
Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME
TypeBlog PostPublishedJune 6, 2019, 12:41 p.m....in mammalian hosts Article contributed by Lukas Morgan Listen to the acoustic reporter gene podcast segment...the body. When a microbe travels through a host organism, the expression of some genes can change depending...protein structure that water-bound photosynthetic organisms use for buoyancy. These proteins allow dissolved... -
Cancer, Inflammation and Immunity - Harnessing the Body’s Defenses to Fight Cancer
TypeBlog PostPublishedJuly 6, 2017, 1:59 p.m....inflammation for therapeutic benefit ”as stated by the organizers. My overall impression from this conference...with the scientific community, contact deposit@addgene.org to find out more about our free deposit and distribution...: Deciphering the Relationship Resources on Addgene.org Browse Cancer Plasmids Browse Immunology Plasmids... -
A Quick Guide to a Career in Software Product Management
TypeBlog PostPublishedNov. 24, 2020, 2:15 p.m....stakeholders, the product managers collate and organize that information and form it into a roadmap--a... features, programs, or tools to build. After organizing the information, a software product manager works...everything running smoothly, you’ll want to be really organized. Often, product managers will have three or more... -
How to Keep a Lab Notebook for Bioinformatic Analyses
TypeBlog PostPublishedJune 2, 2016, 2:30 p.m.... making similar mistakes in the future. Stay organized Set up a table of contents in the top directory...Blog Read Our Tips to Keep Your Lab Notebook Organized Learn about Software You Can Use to Save Time ...Software Matchmaker Additional Resources on Addgene.org Find Protocols for Your Next Experiment Find... -
3 Challenges in Plant Synthetic Biology
TypeBlog PostPublishedJuly 22, 2014, 5:46 p.m....engineer a specific function in these multicellular organisms. However, the rewards are great if you take the...are needed to increase the body of knowledge. Organizations like Open Plant can then build on this research...plant circadian clock. Petunias have been a model organism for plant research for decades, and the biochemical... -
Plasmids 101: Yeast Vectors
TypeBlog PostPublishedFeb. 25, 2014, 7:11 p.m....defined as the inability of an organism to synthesize a particular organic compound required for its growth...DNA transformation system.Unlike most other microorganisms, yeast have both a stable haploid and diploid... -
Form Your Own Peer Mentoring Group: A How-To Guide for Scientists
TypeBlog PostPublishedFeb. 18, 2014, 4:22 p.m....like to start a group mentoring program at your organization, contact me! I can provide a step-by-step, how-to... people from different labs, departments or organizations as it brings a diversity of perspective. Sometimes...transition. Schedule your first meeting and get organized. Discuss your general areas of interest and what... -
Transferable Skills Guide: Public Speaking
TypeBlog PostPublishedApril 20, 2023, 4 p.m.... Should I go over this slide again? Say, 'Oh, I forgot to mention X, so let's quickly go over it now'?...Chinese undergrads. You get nervous and freeze, or forget a point, or talk extremely quickly. You realize...happens to other people, it’s normal and quickly forgotten, and when it happens to you, it’s noteworthy and... -
All in a Twist: dsRNA
TypeBlog PostPublishedFeb. 2, 2023, 2:15 p.m....previous chemical versions with toxicity to many organisms. Fig. 1: Exogenous sources of dsRNA ...cellular dysregulation) is the mitochondria. These organelles have their own circular genomes which can produce...consequences. Nat Rev Mol Cell Biol, 23: 286-301 (2022). doi.org/10.1038/s41580-021-00430-1 More resources on the... -
Antibodies 101: Polyclonal Antibodies
TypeBlog PostPublishedJuly 14, 2021, 1:15 p.m.... antibodies. BioTechniques 65:127–136. https://doi.org/10.2144/btn-2018-0065 Fishman JB, Berg EA (2019...Spring Harb Protoc 2019:pdb.top099101. https://doi.org/10.1101/pdb.top099101 Additional resources on the...recombinant plasmid-based antibodies Resources on Addgene.org Discover Addgene’s antibody plasmid collection... -
Visualizing Protein Turnover In Situ
TypeBlog PostPublishedJan. 16, 2018, 3:20 p.m....in Molecular Biology. This annual symposium is organized by international PhD students at the Max Planck...concerts and bake. you can contact her at rojas@ti2learning.org. References 1. Dieterich, Daniela C., et al... tagging strategies Additional Resources on Addgene.org Fluorescent protein plasmids Use FRET to study... -
Celebrating 15 Years of Scientific Sharing
TypeBlog PostPublishedJan. 14, 2019, 2:08 p.m....celebrate! As we enter this new growth phase of the organization and the expansion of our impact, I’d like to...service and contribution! Leadership On an organizational level, we keep passing our audits with flying...even via Twitter. We love working with local organizations like Just-a-Start, the Northeastern co-op program... -
Choosing the B(right)est Fluorescent Protein: Aggregation Tendency
TypeBlog PostPublishedJune 15, 2017, 2:30 p.m....antiparallel configuration), typical structures known as Organized Smooth Endoplasmic Reticulum (OSER) whorls are...Besides the data, new plasmids and/or cells or organisms that are engineered can be shared to simplify ... Protein Biosensors Additional Resources on Addgene.org Find Empty Backbones with Fluorescent Tags Find... -
Lighting Up Cell Signaling with Photoswitchable Kinases
TypeBlog PostPublishedJuly 25, 2017, 1:20 p.m.... a signaling pathway in the context of a whole organism, but also allows for precise activation of a kinases..., but they need to be expressed in a tissue or organism that blue and violet light can penetrate. How ...Control Protein Activity with GFP Resources at Addgene.org Find KTR reporter plasmids Find photoactivatable... -
Seven Tips for Using LinkedIn as a Scientist
TypeBlog PostPublishedOct. 1, 2019, 1:21 p.m....More…” menu. You can also follow companies and organizations to see their news and job postings. 4) Use ...connections can connect you to people at that organization. This type of connection can help you gather...that is not relevant to business or volunteer organizational work. LinkedIn is not the platform for politics... -
Channelrhodopsins with improved light sensitivity for minimally-invasive optogenetics
TypeBlog PostPublishedNov. 3, 2020, 2:15 p.m....inside a mouse’s brain. But the brain is a tricky organ to access, which often forces scientists to use ...optogenetics. Nat Methods 16:1176–1184 . https://doi.org/10.1038/s41592-019-0583-8 Additional resources ...chemogenetics and optogenetics Resources on Addgene.org Browse all viral vectors available at Addgene... -
Antibodies 101: Choosing the Right Isotype
TypeBlog PostPublishedApril 6, 2023, 3 p.m....Issue 8, pp. 977–983). SAGE Publications. https://doi.org/10.1177/002215549804600813 Erickson, P. A., Lewis... Cell Biology (pp. 283–310). Elsevier. https://doi.org/10.1016/s0091-679x(08)60255-1 Manning, C. F., ...e38313). Public Library of Science (PLoS). https://doi.org/10.1371/journal.pone.0038313 More resources from... -
Making CRISPR Plasmids Using Fragmid
TypeBlog PostPublishedAug. 29, 2024, 1:15 p.m....available. Table 1: Fragmid destination vectors Organism Delivery Type Destination Vector Mammalian...select the features of your vector, which are: Organism (Mammalian or drosophila) Delivery type (lentivirus...technologies. Cell Genomics, 4(3), 100519. https://doi.org/10.1016/j.xgen.2024.100519 More resources from ...