We narrowed to 762 results for: SOM

...journals as those that did make deposits to Addgene.  Insomuch as different journals represent different research...using these weighted numbers. Note: this does drop some journals from our dataset, since not all journals...

... look to nature: some protists are coated in symbiotic bacteria (called epixenosomes) that contain an ...

...Our recent paper (Richardson et al 2016) revealed some of the biophysical parameters that can influence...genetic diseases in which sequence replacement holds some kind of fitness advantage but error-prone repair...

...' NAAAAC  The synthetic guide RNA or gRNA (sometimes sgRNA) In the native Type II CRISPR/Cas system...sequence) to modify the desired genomic locus.   Some historical notes CRISPR arrays in bacterial genomes...

...assignment that was open ended... it was actually something bordering on research, rather than 'here’s the..., sharing ethic that Addgene has behind it is something that very much resonates with me.” About Addgene...

...a process called transfection. Transfection is somewhat comparable to bacterial transformation (the introduction...large-T antigen (293E or 293T cells), allow for episomal amplification of plasmids containing the viral...

...Bioluminescent reporters require a chemical substrate (and sometimes cofactors) to be provided and are generally dimmer...as their substrate is available. Table 1 compares some general advantages and disadvantages of each approach...

...adjusted voltage and compensated your colors. Here are some good practice tips on adjusting voltage: During ...would be shifted to the right in comparison, say somewhere between a 104–105 signal intensity. If your samples...

... gating your target population (b). There is some debate in the literature as to when you need isotype...them to gate our populations, but others do not. Some journals require isotype controls, while others ...

...activate many GPCRs are still unknown. To identify some of these unknown ligands and their GPCR binding ...off-target effects thus scientists then modified some GPCRs further to respond to specific, pharmacologically-inert...

...Technologies. If the first thing you hear when someone says they’re a software product manager is “project...sequencing (NGS), and high-throughput screening. While some developers had science backgrounds, most did not...

...Find plasmids for blue-white screening at Addgene! Some bacterial strains contain lacZ in their genome, ...sequencing primers in our Molecular Biology Reference. Sometimes common  primers can't provide all of the information...

... should be included and what basic information someone new to antibodies might need to successfully start...separate from the author, and a second review from someone less familiar with the subject for clarity. Our...

...cells (Allen, et al., Frontiers in Genome Editing).  Some of the RNA stabilizing modifications listed above...applications like siRNA and antisense oligonucleotides. Some of these synthetic modifications, specifically ones...

...constant domains, and the hinge region length. Now for some essential isotype nomenclature. Antibody heavy chain... the lower-case Greek letters μ, δ, γ, ε, and α. Some isotypes have different versions or subclasses that...

...number of emerging viral applications and give you some recommendations for putting viruses to work. Viral...targeting an exon in your gene of interest, for example. Some customization is required, but many viral constructs...

...we will focus on the most recent advances and how some of these variants may be appropriate for your specific...these off-target effects can be genome-wide and, in some cases, difficult to determine, the potential for...

...travels through a host organism, the expression of some genes can change depending on the location. Current...reporters have limited deep-tissue performance, and sometimes require an alternative such as a radioactive tracer...

...RNA sequence (in this case, norovirus RNA), the ribosome binding site is inaccessible. When the target ...however, it binds to the switch and exposes its ribosome binding site, allowing for the gene on the toehold...

... difficult to replicate. Unclear methods Until someone attempts to replicate the results of another’s ...original research finding we can be more certain that some important, previously unknown variable actually ...

...a lot of space and don’t tell you why you did something or what the result was, so try not to do this.... that I try to follow in my own research. Again, some of this may not apply or may not work for you. The...

...abilities to turn genes on and off,” he said. “Some are strong and some more moderate; there is an allelic series...

...like a lot of specialized science, these tools sometimes have limited distribution. We hope that will change...navigate when mapping out a new project. We’ve shared some of the concerns that Revolution Bio had to address...

...explained that the data must be reproducible by someone else. Second, "an entrepreneur must be OK with ...importantly, “the company does not become real until someone takes the risk full-time and abandons all safety...

... a homologous template provided by the sister chromosome. As cells take those two ends and put them back...there is a possibility of introducing a mutation somewhere else in the genome. It remains to be seen which...

...components. Nearly all of us played with LEGO as a child (some of us still do), so we are used to the idea of general...test out lots of combinations, to end up finding something surprisingly useful. To apply these principles...

...by offering advice on areas where you might need some help. Today in our transferable skills guide: Problem-Solving... question you have about the world. You might do some background research to better understand it and ...

... a month or so of meetings, I began to notice something: even though our scientists and engineers were...versa), then you have almost certainly experienced some of the hurdles I needed to overcome.  I eventually...

...order aggregates. This property can be tolerated in some applications (e.g. labeling of cells or tracking...uploading it as a preprint, or documenting it in some other way. Besides the data, new plasmids and/or...

...light. Any fluorescent molecule will photobleach at some point. For live-cell imaging, it is desirable to...different ways. We describe a number of issues with some of the experimental designs used to make these measurements...