Skip to main content
Addgene
Showing: 481 - 510 of 745 results

  1. GPCRs: How Do They Work and How Do We Study Them?

    Type
    Blog Post
    Published
    Feb. 11, 2021, 2:15 p.m.
    ...activate many GPCRs are still unknown. To identify some of these unknown ligands and their GPCR binding ...off-target effects thus scientists then modified some GPCRs further to respond to specific, pharmacologically-inert...

  2. Viral Vectors 101: Viral Applications

    Type
    Blog Post
    Published
    April 13, 2023, 1:15 p.m.
    ...number of emerging viral applications and give you some recommendations for putting viruses to work. Viral...targeting an exon in your gene of interest, for example. Some customization is required, but many viral constructs...

  3. Flow Cytometry Readouts: Yes, No, and Everything in Between

    Type
    Blog Post
    Published
    Aug. 20, 2024, 1:15 p.m.
    ...adjusted voltage and compensated your colors. Here are some good practice tips on adjusting voltage: During ...would be shifted to the right in comparison, say somewhere between a 104–105 signal intensity. If your samples...

  4. Antibodies 101: Flow Cytometry Controls

    Type
    Blog Post
    Published
    June 6, 2024, 1:50 p.m.
    ... gating your target population (b). There is some debate in the literature as to when you need isotype...them to gate our populations, but others do not. Some journals require isotype controls, while others ...

  5. How Addgene Creates Content

    Type
    Blog Post
    Published
    Nov. 17, 2022, 2:15 p.m.
    ... should be included and what basic information someone new to antibodies might need to successfully start...separate from the author, and a second review from someone less familiar with the subject for clarity. Our...

  6. A Quick Guide to a Career in Software Product Management

    Type
    Blog Post
    Published
    Nov. 24, 2020, 2:15 p.m.
    ...Technologies. If the first thing you hear when someone says they’re a software product manager is “project...sequencing (NGS), and high-throughput screening. While some developers had science backgrounds, most did not...

  8. How to Keep a Lab Notebook for Bioinformatic Analyses

    Type
    Blog Post
    Published
    June 2, 2016, 2:30 p.m.
    ...a lot of space and don’t tell you why you did something or what the result was, so try not to do this.... that I try to follow in my own research. Again, some of this may not apply or may not work for you. The...

  10. 3 Challenges in Plant Synthetic Biology

    Type
    Blog Post
    Published
    July 22, 2014, 5:46 p.m.
    ...like a lot of specialized science, these tools sometimes have limited distribution. We hope that will change...navigate when mapping out a new project. We’ve shared some of the concerns that Revolution Bio had to address...

  12. Kiran Musunuru on the Newest TALEN Genome-Editing System

    Type
    Blog Post
    Published
    Dec. 10, 2013, 3:13 p.m.
    ... a homologous template provided by the sister chromosome. As cells take those two ends and put them back...there is a possibility of introducing a mutation somewhere else in the genome. It remains to be seen which...

  14. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

    Type
    Blog Post
    Published
    June 15, 2017, 2:30 p.m.
    ...order aggregates. This property can be tolerated in some applications (e.g. labeling of cells or tracking...uploading it as a preprint, or documenting it in some other way. Besides the data, new plasmids and/or...

  15. Choosing the B(right)est Fluorescent Protein: Photostability

    Type
    Blog Post
    Published
    June 8, 2017, 2:30 p.m.
    ...light. Any fluorescent molecule will photobleach at some point. For live-cell imaging, it is desirable to...different ways. We describe a number of issues with some of the experimental designs used to make these measurements...

  16. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

    Type
    Blog Post
    Published
    Dec. 8, 2016, 3:30 p.m.
    ...strains ultimately perform the same function, there is some evidence that ccdB Survival™ can be more difficult...plasmid CcdB protein involves poisoning of DNA-topoisomerase II complexes.” J Mol Biol. 1992 Aug 5;226(3)...

  17. Transferable Skills Guide: Problem Solving

    Type
    Blog Post
    Published
    Nov. 30, 2021, 2:15 p.m.
    ...by offering advice on areas where you might need some help. Today in our transferable skills guide: Problem-Solving... question you have about the world. You might do some background research to better understand it and ...

  18. Plasmids 101: The protein expression toolbox

    Type
    Blog Post
    Published
    Feb. 16, 2023, 7:05 p.m.
    ...protein expression control tools as well as explore some new innovations. Whether you want to turn things...more difficult than a simple knock out. Looking for some permanent knock out plasmids? Check out our Cre ...

  19. Giving gRNAs a Facelift - Synthetic and Beyond

    Type
    Blog Post
    Published
    Oct. 20, 2022, 1:15 p.m.
    ...cells (Allen, et al., Frontiers in Genome Editing).  Some of the RNA stabilizing modifications listed above...applications like siRNA and antisense oligonucleotides. Some of these synthetic modifications, specifically ones...

  20. Transferable Skills Guide: Cross-team Communication

    Type
    Blog Post
    Published
    Nov. 21, 2019, 1:53 p.m.
    ... a month or so of meetings, I began to notice something: even though our scientists and engineers were...versa), then you have almost certainly experienced some of the hurdles I needed to overcome.  I eventually...

  22. Plasmids 101: Golden Gate Cloning

    Type
    Blog Post
    Published
    Aug. 27, 2015, 2:30 p.m.
    ...Gate cloning into a modular cloning strategy. Sometimes referred to as MoClo, this strategy uses the Type...Christian M, Wang L, Zhang Y, Schmidt C, Baller JA, Somia NV, Bogdanove AJ, Voytas DF. Nucleic Acids Res. ...

  23. Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

    Type
    Blog Post
    Published
    Sept. 26, 2019, 12:50 p.m.
    ...methods aren’t stealthy enough to gain access to some cells and tissues, such as human induced pluripotent...zygotes with CRISPR components. However, this sometimes requires intracellular injections into the cytoplasm...

  24. Open Resources and Plasmid Tools For Studying C. elegans

    Type
    Blog Post
    Published
    July 18, 2019, 12:55 p.m.
    ...experiments.  In this blog post let's take a look at some of the other open resources discussed at the meeting... (Schwartz and Jorgensen, 2018).  These are just some examples of worm resources and tools openly shared...

  26. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

    Type
    Blog Post
    Published
    July 12, 2021, 1:15 p.m.
    ... from Maya Kostman for the IGI.     Here are some highlights of the plasmids shared over the last ... plasmid sharing and more. As you’ve read above, some of these plasmids have been in the repository for...

  27. High Complexity Golden Gate Assembly with NEB’s DAD

    Type
    Blog Post
    Published
    Aug. 1, 2023, 1:15 p.m.
    ...to select large sets (with overhangs that violate some of the traditional design rules) for successful ...deposited by NEB – and don’t forget to ask DAD for some help! References Potapov, V., Ong, J. L., Kucera...

  28. Viral Vectors 101: Chemogenetics

    Type
    Blog Post
    Published
    July 11, 2023, 1:15 p.m.
    ...ability to be metabolized into clozapine (CLZ). While some scientists argue that these issues can be mitigated...These synthetic agonists can penetrate the brain and some, such as varenicline, are already approved for clinical...

  29. Science communication: 9 Strategies to Get Your Foot in the Door

    Type
    Blog Post
    Published
    Sept. 27, 2017, 1:23 p.m.
    ...visual person. Why don’t you record a podcast or put some effort into making a video? Use your unique talent...annual meetings. 8) Training – Get a formal education Some people get into science communication by taking ...

  30. The Materials Science of Optogenetics Experiments

    Type
    Blog Post
    Published
    Sept. 17, 2015, 2:30 p.m.
    ... of reasons for this but the primary one is that some behavioral experiments require stimulation only ... in this primer on optogenetics we’ll delve into some of the important considerations when performing ...
Showing: 481 - 510 of 745 results