Skip to main content

We narrowed to 762 results for: SOM

Showing: 481 - 510 of 762 results
  1. Summer SciComm Series: A PhD in Science Communication

    Type
    Blog Post
    Published
    Aug. 23, 2022, 1:15 p.m.
    ... an experiment, then spend the evening listen to some bloke at the pub telling me that actually he drives...might struggle to understand. Why should we trust something that we cannot read or verify for ourselves? Further...
  2. Optimizing Donor DNA for Enhanced CRISPR Genome Editing

    Type
    Blog Post
    Published
    March 24, 2016, 2:30 p.m.
    ...Our recent paper (Richardson et al 2016) revealed some of the biophysical parameters that can influence...genetic diseases in which sequence replacement holds some kind of fitness advantage but error-prone repair...
  3. Components of CRISPR/Cas9

    Type
    Blog Post
    Published
    Feb. 2, 2016, 5 p.m.
    ...' NAAAAC  The synthetic guide RNA or gRNA (sometimes sgRNA) In the native Type II CRISPR/Cas system...sequence) to modify the desired genomic locus.   Some historical notes CRISPR arrays in bacterial genomes...
  4. Addgene Welcomes John Doench to the Advisory Board

    Type
    Blog Post
    Published
    Aug. 13, 2015, 2:30 p.m.
    ...assignment that was open ended... it was actually something bordering on research, rather than 'here’s the..., sharing ethic that Addgene has behind it is something that very much resonates with me.” About Addgene...
  5. Plasmids 101: Mammalian Vectors

    Type
    Blog Post
    Published
    March 25, 2014, 3:15 p.m.
    ...a process called transfection. Transfection is somewhat comparable to bacterial transformation (the introduction...large-T antigen (293E or 293T cells), allow for episomal amplification of plasmids containing the viral...
  6. Plasmids 101: Screening Strategies Used in Plasmid Cloning

    Type
    Blog Post
    Published
    Sept. 15, 2020, 1:15 p.m.
    ...Find plasmids for blue-white screening at Addgene! Some bacterial strains contain lacZ in their genome, ...sequencing primers in our Molecular Biology Reference. Sometimes common  primers can't provide all of the information...
  7. Viral Vectors 101: Viral Applications

    Type
    Blog Post
    Published
    April 13, 2023, 1:15 p.m.
    ...number of emerging viral applications and give you some recommendations for putting viruses to work. Viral...targeting an exon in your gene of interest, for example. Some customization is required, but many viral constructs...
  8. Flow Cytometry Readouts: Yes, No, and Everything in Between

    Type
    Blog Post
    Published
    Aug. 20, 2024, 1:15 p.m.
    ...adjusted voltage and compensated your colors. Here are some good practice tips on adjusting voltage: During ...would be shifted to the right in comparison, say somewhere between a 104–105 signal intensity. If your samples...
  9. Antibodies 101: Flow Cytometry Controls

    Type
    Blog Post
    Published
    June 6, 2024, 1:50 p.m.
    ... gating your target population (b). There is some debate in the literature as to when you need isotype...them to gate our populations, but others do not. Some journals require isotype controls, while others ...
  10. Antibodies 101: Isotypes

    Type
    Blog Post
    Published
    Oct. 19, 2021, 1:15 p.m.
    ...constant domains, and the hinge region length. Now for some essential isotype nomenclature. Antibody heavy chain... the lower-case Greek letters μ, δ, γ, ε, and α. Some isotypes have different versions or subclasses that...
  11. Fluorescent Proteins 101: Luciferases

    Type
    Blog Post
    Published
    July 22, 2025, 1:15 p.m.
    ...Bioluminescent reporters require a chemical substrate (and sometimes cofactors) to be provided and are generally dimmer...as their substrate is available. Table 1 compares some general advantages and disadvantages of each approach...
  12. How Addgene Creates Content

    Type
    Blog Post
    Published
    Nov. 17, 2022, 2:15 p.m.
    ... should be included and what basic information someone new to antibodies might need to successfully start...separate from the author, and a second review from someone less familiar with the subject for clarity. Our...
  13. Giving gRNAs a Facelift - Synthetic and Beyond

    Type
    Blog Post
    Published
    Oct. 20, 2022, 1:15 p.m.
    ...cells (Allen, et al., Frontiers in Genome Editing).  Some of the RNA stabilizing modifications listed above...applications like siRNA and antisense oligonucleotides. Some of these synthetic modifications, specifically ones...
  14. GPCRs: How Do They Work and How Do We Study Them?

    Type
    Blog Post
    Published
    Feb. 11, 2021, 2:15 p.m.
    ...activate many GPCRs are still unknown. To identify some of these unknown ligands and their GPCR binding ...off-target effects thus scientists then modified some GPCRs further to respond to specific, pharmacologically-inert...
  15. A Quick Guide to a Career in Software Product Management

    Type
    Blog Post
    Published
    Nov. 24, 2020, 2:15 p.m.
    ...Technologies. If the first thing you hear when someone says they’re a software product manager is “project...sequencing (NGS), and high-throughput screening. While some developers had science backgrounds, most did not...
  16. How to Keep a Lab Notebook for Bioinformatic Analyses

    Type
    Blog Post
    Published
    June 2, 2016, 2:30 p.m.
    ...a lot of space and don’t tell you why you did something or what the result was, so try not to do this.... that I try to follow in my own research. Again, some of this may not apply or may not work for you. The...
  17. 3 Challenges in Plant Synthetic Biology

    Type
    Blog Post
    Published
    July 22, 2014, 5:46 p.m.
    ...like a lot of specialized science, these tools sometimes have limited distribution. We hope that will change...navigate when mapping out a new project. We’ve shared some of the concerns that Revolution Bio had to address...
  18. Kiran Musunuru on the Newest TALEN Genome-Editing System

    Type
    Blog Post
    Published
    Dec. 10, 2013, 3:13 p.m.
    ... a homologous template provided by the sister chromosome. As cells take those two ends and put them back...there is a possibility of introducing a mutation somewhere else in the genome. It remains to be seen which...
  19. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

    Type
    Blog Post
    Published
    June 15, 2017, 2:30 p.m.
    ...order aggregates. This property can be tolerated in some applications (e.g. labeling of cells or tracking...uploading it as a preprint, or documenting it in some other way. Besides the data, new plasmids and/or...
  20. Choosing the B(right)est Fluorescent Protein: Photostability

    Type
    Blog Post
    Published
    June 8, 2017, 2:30 p.m.
    ...light. Any fluorescent molecule will photobleach at some point. For live-cell imaging, it is desirable to...different ways. We describe a number of issues with some of the experimental designs used to make these measurements...
  21. Plasmids 101: The protein expression toolbox

    Type
    Blog Post
    Published
    Feb. 16, 2023, 7:05 p.m.
    ...protein expression control tools as well as explore some new innovations. Whether you want to turn things...more difficult than a simple knock out. Looking for some permanent knock out plasmids? Check out our Cre ...
  22. Transferable Skills Guide: Problem Solving

    Type
    Blog Post
    Published
    Nov. 30, 2021, 2:15 p.m.
    ...by offering advice on areas where you might need some help. Today in our transferable skills guide: Problem-Solving... question you have about the world. You might do some background research to better understand it and ...
Showing: 481 - 510 of 762 results