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  1. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    Published
    Aug. 13, 2024, 1:15 p.m.
    ...Antibodies Polyclonal Antibodies Resources on addgene.org Addgene's Antibody Purification Protocol Addgene's..., 2014, Pages 438-449, ISSN 1359-0294, https://doi.org/10.1016/j.cocis.2014.10.002. Randolph TW, Schiltz...

  2. A Guide to Starting Your Own Journal Club

    Type
    Blog Post
    Published
    Feb. 6, 2020, 3 p.m.
    ...figure. Some follow the same figure by figure organization with a different person responsible for each...journal clubs should be well planned and carefully organized. Most importantly, they should be regularly assessed...

  3. AAVs CREATed for Gene Delivery to the CNS and PNS

    Type
    Blog Post
    Published
    Sept. 28, 2017, 2:01 p.m.
    ..., which connect the heart, lung, gut, and other organs to the CNS, are also an important target for gene...Viral Vector Blog Posts Additional Resources on Addgene.org Search for AAV Vectors to use with your research...

  4. Tips for arabidopsis transformation

    Type
    Blog Post
    Published
    Oct. 25, 2018, 1:23 p.m.
    ...Stanford University. Arabidopsis is a fantastic model organism for many reasons, not the least of which is ease...Using arabidopsis in the classroom Resources on Addgene.org Find plant plasmids Visit our plant resource...

  5. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    Published
    July 31, 2018, 12:55 p.m.
    ... as an ideal target. It is an easily accessible organ that can be monitored non-invasively over time. ...for gene expression in mammals Resources at Addgene.org Find AAV plasmids Find ready-to-use AAV viral...

  6. Tips for Screening with Yeast Two Hybrid Systems

    Type
    Blog Post
    Published
    Oct. 22, 2015, 2:30 p.m.
    ...generated using random genomic fragments from the organism of interest, or a defined cDNA library (e.g. the...number of two-hybrid systems available in model organisms, including bacteria, alternative fungi (C. albicans...

  7. Your Lentiviral Plasmid FAQs Answered

    Type
    Blog Post
    Published
    April 23, 2014, 1:08 p.m.
    ...Read about the ClonTracr Library Resources on Addgene.org Addgene's most popular lentiviral plasmids Overview... Kendall Morgan...

  8. Hot Plasmids - November 2023

    Type
    Blog Post
    Published
    Nov. 14, 2023, 3:35 a.m.
    ...design. Nature Communications, 14(1), 5141. https://doi.org/10.1038/s41467-023-40788-8. PMID 37612276.   Optogenetic...proximity labeling experiments in cells, tissues, and organisms. Lee, S.Y., Cheah, J.S., et al. (2023). Engineered...

  9. Viral Production at Addgene

    Type
    Blog Post
    Published
    Oct. 24, 2023, 1:15 p.m.
    ...Gene Therapy Methods, 25(2), Article 2. https://doi.org/10.1089/hgtb.2013.131 Wang, Y., Bergelson, S., ...Human Gene Therapy Methods, 29(2), 96–103. https://doi.org/10.1089/hgtb.2017.198  ...

  10. New CRISPR Tools: Cas7-11 and PASTE

    Type
    Blog Post
    Published
    Feb. 17, 2022, 2 p.m.
    ...integrases bioRxiv 2021. 11.01.466786; doi: https://doi.org/10.1101/2021.11.01.466786 Additional Resources ... With Cas13  CRISPR Cheat Sheet Resources at Addgene.org CRISPR Guide...

  11. Transferable Skills Guide: Creativity

    Type
    Blog Post
    Published
    Jan. 18, 2022, 2:15 p.m.
    ...Review. Retrieved January 14, 2022, from https://hbr.org/2019/11/5-mental-mistakes-that-kill-your-productivity...Review. Retrieved January 14, 2022, from https://hbr.org/2019/07/a-brief-exercise-to-spur-innovation-on-...

  12. Transferable Skills Guide: Career Planning Resources

    Type
    Blog Post
    Published
    Nov. 14, 2017, 2 p.m.
    ...Chemical Society (ACS) are just 2 examples of organizations that hold large, annual conferences. Gordon ...Management Advice from Joanne Kamens Resources at Addgene.org Find Open Positions at Addgene ...

  14. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

    Type
    Blog Post
    Published
    Nov. 1, 2018, 12:35 p.m.
    ...toxins to claim territory or kill competing microorganisms, but did you know bacteria also produce substances...about knockout/knock-in plasmids Resources on Addgene.org Check out our molecular biology reference Search...

  15. Healthcare Consulting: A Door to the Business of Life Sciences

    Type
    Blog Post
    Published
    Oct. 13, 2016, 2:30 p.m.
    ...pharmaceutical companies, nonprofit or global health organizations, investment and equity research firms, biotech...Entrepeneur Get Tips on Resume Writing Resources on Addgene.org Check Out Career Opportunities at Addgene ...

  16. Tips for Getting a Faculty Position

    Type
    Blog Post
    Published
    July 20, 2023, 1:15 p.m.
    ...please do not hesitate to email me at snappe@janelia.hhmi.org." You can find his original blog post below... Series Science Career Options Resources on Addgene.org Learn more About Careers at Addgene ...

  17. CRISPR 101: Homology Directed Repair

    Type
    Blog Post
    Published
    Jan. 26, 2023, 2:15 p.m.
    ...). PubMed. Making designer mutants in model organisms. Peng, Y. et al. Development 141; (2014). PubMed...CRISPR Editing in Human Cells Resources on Addgene.org Browse All CRISPR Plasmids Find Validated gRNAs...

  18. Evolution of Lab Techniques

    Type
    Blog Post
    Published
    June 21, 2016, 2:30 p.m.
    ... abundance. If a scientist wants to know if an organism has a particular gene mutation, southern blotting...Check out Plasmid Cloning by PCR Resources on Addgene.org Check out Our Protocol Pages Find Molecular ...

  19. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

    Type
    Blog Post
    Published
    May 10, 2016, 2:30 p.m.
    ...difficult given that the viral reservoir spans multiple organ systems. Both approaches may prove more successful...Genome-Wide Screens Additional Resources onf Addgene.org Check out Addgene’s newly updated CRISPR Guide...

  20. 3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells

    Type
    Blog Post
    Published
    Sept. 5, 2017, 1:58 p.m.
    ...versatile tool for precise gene editing in many organisms and model systems. We have used CRISPR/Cas9 extensively...Delivering CRISPR Ribonucleoproteins Resources on Addgene.org Browse All CRISPR Plasmids Check Out Our CRISPR...

  21. Harnessing Bacterial Toxins for Allelic Exchange

    Type
    Blog Post
    Published
    Aug. 15, 2019, 12:30 p.m.
    ...s toolbox. Depending on the growth rate of the organism of interest, from the day primers arrive, the ...about Lambda Red recombineering Resources on Addgene.org Find plasmids for allelic exchange Read our ...

  23. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    Published
    Jan. 10, 2023, 2:15 p.m.
    ... in the comments below or contact us at help@addgene.org. Resources and References References Bacolla...19408315; PMCID: PMC2735187. More resources on Addgene.org  Addgene's Plasmids 101 eBookAddgene's Molecular...

  24. Is this the right place for me? 8 tactics for choosing a lab

    Type
    Blog Post
    Published
    Oct. 2, 2018, 12:56 p.m.
    ...encourage diversity in all forms? How are their organizational skills? Are they involved with colleagues ...posts on mentoring for scientists Resources on Addgene.org Learn about open positions at Addgene Check ...

  25. Finding Your Perfect Job After University

    Type
    Blog Post
    Published
    Jan. 12, 2016, 3:30 p.m.
    ...Luckily, I have worked for a variety of different organizations, so I tend to provide them with an overview ...practices, but can sometimes go unnoticed at large organizations. Another downside to large commercial companies...

  27. Important Considerations When Using AAVs

    Type
    Blog Post
    Published
    June 13, 2017, 2:30 p.m.
    ..., AAV7, AAV8, AAV9 Adapted from https://www.addgene.org/viral-vectors/aav/aav-guide. Where in the tissue...Viral Vector Elements Additional Resources on Addgene.org Find AAV Vectors for Your Research Get Ready-to-Use...

  28. Antibodies 101: Designing Your First Flow Panel

    Type
    Blog Post
    Published
    May 28, 2024, 1:45 p.m.
    ...different in nature (eGFP being a protein and FITC an organic molecule), their bleed is so strong that these ...Antibodies 101: Flow Compensation Resources on Addgene.org Addgene's Antibody Guide Ready-to-use antibodies...

  29. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    Published
    July 19, 2016, 2:30 p.m.
    ...unaffected by intervening proteins, lipid bilayers, organelles or other cellular impediments. FRET has been ...Your Fluorescent Protein Basics Resources on Addgene.org Find Plasmids for FRET Browse Empty Plasmids...

  30. Custom CRISPR Screens & the Green Listed Software

    Type
    Blog Post
    Published
    July 11, 2017, 2:30 p.m.
    ...melanogaster, and T. gondii are available through www.addgene.org/crispr/libraries/. Additional libraries are...gRNA Tips for a 1st CRISPR User Resources on Addgene.org Learn More About CRISPR Find CRISPR Screening...
Showing: 481 - 510 of 795 results