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Showing: 481 - 510 of 814 results
  1. Antibodies 101: Stripping and Reprobing Western Blots

    Type
    Blog Post
    Published
    Aug. 1, 2024, 1:15 p.m.
    ...antibody you used to assess the stripping, and don’t forget to re-block your membrane! Depending on the system...out other Antibody 101 articles Resources on addgene.org Western Blot Protocol Western Blot Protocol Video...
  2. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

    Type
    Blog Post
    Published
    April 6, 2021, 12:30 p.m.
    ...Extracts. Human Gene Therapy 31:664–678 . https://doi.org/10.1089/hum.2019.277 Joung J, Konermann S, Gootenberg...activation screening. Nat Protoc 12:828–863 . https://doi.org/10.1038/nprot.2017.016...
  3. Adeno Associated Virus (AAV) for Cell and Gene Therapy

    Type
    Blog Post
    Published
    Nov. 7, 2017, 1:59 p.m.
    ...investigated including lentiviruses, adenovirus, and inorganic delivery tools such as nanoparticles. These technologies...Addgene AAV Quality Control Process Resources on Addgene.org Find ready-to-use AAV in Addgene's Viral Service...
  4. 6 Tips for Grant Writing

    Type
    Blog Post
    Published
    Oct. 18, 2016, 2:30 p.m.
    ...used to conversing with other scientists that we forget that some of the terminology we use routinely isn...applications for funding from charities, commercial organizations, or universities may have someone senior from...
  5. Tips for Technical Support Calls

    Type
    Blog Post
    Published
    Sept. 20, 2016, 2:39 p.m.
    ... the company’s FAQs? A lot of companies and organizations selling biological reagents have a ton of data...with the Addgene Deposit Process Resources on Addgene.org Check Out Our Frequently Asked Questions Pages...
  6. Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux

    Type
    Blog Post
    Published
    Sept. 13, 2016, 2:30 p.m.
    ...Advocating for Science Symposium and Workshop, organized by Future of Research, Academics for the Future... More information can be found at http://futureofresearch.org/advocating-for-science-boston-2016/. Sign...
  7. Adapting Toehold Switches to Detect Zika Virus

    Type
    Blog Post
    Published
    Aug. 30, 2016, 2:30 p.m.
    ...Guillain-Barré syndrome. In response, the World Health Organization has declared a global health emergency and called...Used to Study Dangerous Viruses Resources on Addgene.org Check out All the Plasmids from the Collin's...
  8. 5 Tips for Troubleshooting Viral Transductions

    Type
    Blog Post
    Published
    Aug. 11, 2016, 2:23 p.m.
    ...PMID: 12036457. 6. Davis, Howard E., Jeffrey R. Morgan, and Martin L. Yarmush. "Polybrene increases retrovirus...Lentiviral Vectors Additional Resources on the Addgene.org Check out Our Viral Vector Guide Pages Find ...
  9. Plasmids 101: Protein Expression

    Type
    Blog Post
    Published
    June 7, 2018, 1:17 p.m.
    ...frequently used species due to its use as a model organism in genetics and biochemistry. When using S. cerevisiae...promoters Check out protein tags Resources on Addgene.org Bacterial expression systems Search for plasmids...
  10. Transferable Skills Guide: Managing a Team

    Type
    Blog Post
    Published
    Jan. 30, 2018, 2:42 p.m.
    ...Positive feedback is equally as important so don’t forget to recognize someone’s hard work and reward a job...Industry and Success in Management Resources at Addgene.org Addgene's Management Team ...
  11. PCR Applications for Copy Number Variation Assays

    Type
    Blog Post
    Published
    April 26, 2022, 1:15 p.m.
    ...Reaction Overview and Applications Resources on Addgene.org Protocol: Polymerase Chain Reaction (PCR) Protocol...and FCGR3B. PloS one, 10(1), e0116791. https://doi.org/10.1371/journal.pone.0116791 Providenti MA, O'Brien...
  12. PhD Applications After COVID

    Type
    Blog Post
    Published
    Jan. 5, 2023, 2:15 p.m.
    ...more comfortable and help you to keep yourself organized. By writing down your key points and keeping them...  Ashley Bastin is a first year PhD student at George Washington University whose research focuses on...
  13. Pooled Library Amplifications

    Type
    Blog Post
    Published
    May 30, 2024, 1:30 p.m.
    ...protocol, feel free to contact us at deposit@addgene.org. Happy amplifying! More resources on the Addgene...Quality Control for Pooled Libraries Resources on Addgene.org Addgene's Guide to Plasmid Pooled Libraries  ...
  14. Addgene's Cancer Collection Pages Connect the Research Community

    Type
    Blog Post
    Published
    Sept. 6, 2016, 2:30 p.m.
    ...proliferation. Hence it is imperative to the health of an organism that both classes of genes function normally to...Used in Cancer Lineage Tracing Resources on Addgene.org Visit our Cancer Collection Pages Deposit Your...
  15. Anatomy of a Plasmid Page at Addgene

    Type
    Blog Post
    Published
    Feb. 4, 2016, 3:30 p.m.
    ... please don't hesitate to contact us at help@addgene.org Resources on the Addgene Blog Read about Our... Do Quality Control at Addgene Resources on Addgene.org Join the Addgene Community Learn the Benefits...
  16. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    Published
    July 31, 2018, 12:55 p.m.
    ... as an ideal target. It is an easily accessible organ that can be monitored non-invasively over time. ...for gene expression in mammals Resources at Addgene.org Find AAV plasmids Find ready-to-use AAV viral...
  17. AAVs CREATed for Gene Delivery to the CNS and PNS

    Type
    Blog Post
    Published
    Sept. 28, 2017, 2:01 p.m.
    ..., which connect the heart, lung, gut, and other organs to the CNS, are also an important target for gene...Viral Vector Blog Posts Additional Resources on Addgene.org Search for AAV Vectors to use with your research...
  18. Uncovering Ancestral Bias in CRISPR Research

    Type
    Blog Post
    Published
    Nov. 29, 2022, 2:15 p.m.
    ...Boehm. bioRxiv 2022.11.18.517155; doi: https://doi.org/10.1101/2022.11.18.517155   Resources on ancestral...diversity. Nature 604, 437–446 (2022). https://doi.org/10.1038/s41586-022-04601-8 Lewis, Anna CF, Santiago...
  19. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    Published
    Aug. 13, 2024, 1:15 p.m.
    ...Antibodies Polyclonal Antibodies Resources on addgene.org Addgene's Antibody Purification Protocol Addgene's..., 2014, Pages 438-449, ISSN 1359-0294, https://doi.org/10.1016/j.cocis.2014.10.002. Randolph TW, Schiltz...
  20. A Guide to Starting Your Own Journal Club

    Type
    Blog Post
    Published
    Feb. 6, 2020, 3 p.m.
    ...figure. Some follow the same figure by figure organization with a different person responsible for each...journal clubs should be well planned and carefully organized. Most importantly, they should be regularly assessed...
  21. Tips for arabidopsis transformation

    Type
    Blog Post
    Published
    Oct. 25, 2018, 1:23 p.m.
    ...Stanford University. Arabidopsis is a fantastic model organism for many reasons, not the least of which is ease...Using arabidopsis in the classroom Resources on Addgene.org Find plant plasmids Visit our plant resource...
  22. Tips for Screening with Yeast Two Hybrid Systems

    Type
    Blog Post
    Published
    Oct. 22, 2015, 2:30 p.m.
    ...generated using random genomic fragments from the organism of interest, or a defined cDNA library (e.g. the...number of two-hybrid systems available in model organisms, including bacteria, alternative fungi (C. albicans...
  23. Your Lentiviral Plasmid FAQs Answered

    Type
    Blog Post
    Published
    April 23, 2014, 1:08 p.m.
    ...Read about the ClonTracr Library Resources on Addgene.org Addgene's most popular lentiviral plasmids Overview... Kendall Morgan...
  24. Transferable Skills Guide: Career Planning Resources

    Type
    Blog Post
    Published
    Nov. 14, 2017, 2 p.m.
    ...Chemical Society (ACS) are just 2 examples of organizations that hold large, annual conferences. Gordon ...Management Advice from Joanne Kamens Resources at Addgene.org Find Open Positions at Addgene ...
  25. New CRISPR Tools: Cas7-11 and PASTE

    Type
    Blog Post
    Published
    Feb. 17, 2022, 2 p.m.
    ...integrases bioRxiv 2021. 11.01.466786; doi: https://doi.org/10.1101/2021.11.01.466786 Additional Resources ... With Cas13  CRISPR Cheat Sheet Resources at Addgene.org CRISPR Guide...
  26. Transferable Skills Guide: Creativity

    Type
    Blog Post
    Published
    Jan. 18, 2022, 2:15 p.m.
    ...Review. Retrieved January 14, 2022, from https://hbr.org/2019/11/5-mental-mistakes-that-kill-your-productivity...Review. Retrieved January 14, 2022, from https://hbr.org/2019/07/a-brief-exercise-to-spur-innovation-on-...
  27. Hot Plasmids - November 2023

    Type
    Blog Post
    Published
    Nov. 14, 2023, 3:35 a.m.
    ...design. Nature Communications, 14(1), 5141. https://doi.org/10.1038/s41467-023-40788-8. PMID 37612276.   Optogenetic...proximity labeling experiments in cells, tissues, and organisms. Lee, S.Y., Cheah, J.S., et al. (2023). Engineered...
Showing: 481 - 510 of 814 results