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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 866 results for: FER

Showing: 641 - 680 of 866 results

  1. Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

    Type
    Blog Post
    Published
    June 12, 2014, 3:30 p.m.
    ...explains it, her work has always been focused on gene transfer, from transgenes to plants, chloroplast to the...anymore. The kit contains sequences for several different fluorescent proteins. Visualizing fluorescence...for those who go to work in cancer biology.  References: A Golden Gate Modular Cloning Toolbox for Plants...

  2. Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

    Type
    Blog Post
    Published
    Dec. 12, 2013, 3:35 p.m.
    ...the physical DNA samples, for when that's the preferred mode of receiving parts." "We have found an amazing...functions, which we know and love. Boolean is in reference to George Boole who in 1854 was thinking about...tell us readers, what do you want to make?   References: Jerome Bonnet et al. "Amplifying genetic logic...

  3. Isolating Genomic Regions of Interest with the CRISPR System

    Type
    Blog Post
    Published
    May 30, 2019, 12:44 p.m.
    ... adaptations of this system have been made in different fields of study. In addition to the identification...purification with an anti-Cas9 antibody. These different tag systems and affinity purification schemes ...please also see our interview with Dr. Fujii. References Campbell, Amy E., et al. "NuRD and CAF-1-mediated...

  4. Capturing a Moment with Marc Zimmer’s “The State of Science”

    Type
    Blog Post
    Published
    July 30, 2020, 1:15 p.m.
    ...journal editorials, science social media, and conference workshops for years. That is, presumably, why...these fields, and seeing names like Feng Zhang, Jennifer Doudna, Karl Deisseroth, and Ed Boyden crop up...scientist who enjoys learning a little bit about many different things (part of the reason Addgene has been such...

  5. Tips for arabidopsis transformation

    Type
    Blog Post
    Published
    Oct. 25, 2018, 1:23 p.m.
    ...plant pathogenic species that stably integrates transfer DNA (tDNA) into the genomes of the plants it attacks... (Nakagawa et al., 2007). Alternatively, if you prefer Golden Gate assembly, there are a series of plasmids...countless transgenic plants in the process.     References 1. Emami, S., Yee, M. and Dinneny, J. R. (2013...

  6. Better Dyeing Through Chemistry & Small Molecule Fluorophores

    Type
    Blog Post
    Published
    Sept. 8, 2016, 2:30 p.m.
    ... lipoic acid ligase, biotin ligase, phosphopantetheinyltransferase) – These enzymes catalyze the covalent... We continue to develop derivatives that have different spectral properties, exhibit high fluorogenicity...modalities. Follow him on twitter @rhodamine110.   References 1. Liu, Zhe, Luke D. Lavis, and Eric Betzig. ...

  7. A Career in Grants: How To Become a Grant Professional

    Type
    Blog Post
    Published
    Aug. 20, 2015, 2:30 p.m.
    ...development, and more; our work touched on many different issues, and our program didn’t stay in one place...management, and program evaluation are highly transferable. Use your experience and your passion(s) to ...coach you. There are many grant consultants who offer grant training, mentoring and coaching services,...

  8. Tips for Writing a Good Cover Letter

    Type
    Blog Post
    Published
    May 21, 2024, 1:30 p.m.
    ...can use your cover letter to explain why your transferable skills make you a good candidate for the position...experience or skills, often by pointing to your transferable skills. In the above example, if you don’t have...experience, having given more than 20 talks at conferences and taught for five years at [name of the university...

  9. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    Published
    Aug. 14, 2018, 12:42 p.m.
    ...tracking experiments (Rizzo, 2009). With many different FPs that emit across the visible spectrum, researchers...antibodies. The “spaghetti monster” name is a reference to the deity of the social movement, the church... to the expanded use of smFPs in the future. References 1. Viswanathan, Sarada, et al. "High-performance...

  10. New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression

    Type
    Blog Post
    Published
    Jan. 24, 2023, 2:15 p.m.
    ... the Shapiro lab at Caltech.   References and Resources References Piraner, D. I. et al. Going Deeper...straightforward to engineer various cell lines from different tissues and mammalian species to produce gas vesicles...

  11. Rett Syndrome: A History of Research and Therapeutic Outlooks

    Type
    Blog Post
    Published
    Nov. 19, 2020, 2:15 p.m.
    ...these discoveries, scientists have studied how different parts of MECP2 influence Rett Syndrome. Zoghbi...varying degrees determine the clinical severity of different MECP2 mutations. More recently, the Bird lab found..., Rett databases, and other helpful tools.  References: Amir RE, Van den Veyver IB, Wan M, Tran CQ, ...

  12. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type
    Blog Post
    Published
    Aug. 20, 2020, 2:30 p.m.
    .... Note: Jennifer Tsang and Mary Gearing contributed to the update of this post. References Abudayyeh...2016). Type VI CRISPR systems, which target RNA, offer additional targeting flexibility. Cas13a (formerly...

  13. CRISPR-mediated Plant Base Editors

    Type
    Blog Post
    Published
    Jan. 3, 2019, 1:35 p.m.
    ...0-5%. This blog post covers a brief review of different basic BE technologies and their adaptation for...Nickase Cas9 (D10A) Deaminase PAM requirements Reference CBE SpCas9 rAPOBEC1 NGG 6 SpCas9 Target-AID...improvement in the laboratory of Yinong Yang at PSU.   References 1. Komor, Alexis C., et al. "Programmable editing...

  14. Plasmids 101: E. coli Strains for Protein Expression

    Type
    Blog Post
    Published
    Feb. 10, 2015, 3:06 p.m.
    ...below, certain specialized strains are available to confer greater transcriptional control, assist with proper...pLys comes in two flavors—pLysS and pLysE—the difference being that the latter provides tighter control...minimal media supplemented with M9 salts may be preferable if the protein product is secreted to the medium...

  15. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    Published
    April 3, 2014, 8:05 p.m.
    ...host organism. Because transcription machinery differs between cell types or organisms, promoters must...eukaryotes and prokaryotes We have included two reference tables below listing some of the most common bacterial...Mammalian Vectors Read about Reporter Gene like Luciferase and GFP Resources on Addgene.org Find Plasmids...

  16. Harnessing Bacterial Toxins for Allelic Exchange

    Type
    Blog Post
    Published
    Aug. 15, 2019, 12:30 p.m.
    ...leaves a “scar” in the chromosome, sometimes interfering with expression of surrounding genes. However...Gram-negative bacteria become resistant to antibiotics! References Espah Borujeni, Amin, Anirudh S. Channarasappa...for allelic exchange Read our molecular biology reference Watch molecular biology protocol videos ...

  17. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    Published
    Oct. 5, 2021, 1:15 p.m.
    ...recent research @daria_gift_shch.     References and resources References Shemetov, A.A., Oliinyk, O.S. & ...the monomeric state of a probe leads to less interference with the oligomeric state of a fusion partner...

  18. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

    Type
    Blog Post
    Published
    May 5, 2020, 1:15 p.m.
    ...Cas12a, Cas13a does not require strict sequence preferences at the target site, while Cas12a requires a PAM...femto-aptomolar range. For more details on the differences between these methods, check out this table.  ...can follow her on Twitter @shra_krishna.       References Azhar, M., Phutela, R., Ansari, A. H., Sinha,...

  19. CRISPR 101: Validating Your Genome Edit

    Type
    Blog Post
    Published
    Nov. 3, 2022, 12:15 p.m.
    ...dice on a new gRNA in the future! References and Resources References Eva K. Brinkman, Tao Chen, Mario ... frame editing frequency is 50%, then you could infer that approximately 25% of cells are likely -/-, ...

  20. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

    Type
    Blog Post
    Published
    May 10, 2016, 2:30 p.m.
    ...binding sites increased activation of HIV promoter-luciferase constructs. In multiple HIV-1 latent T cell lines...the HIV-1 copy number, although the efficiency differed from 48-100% between strains. In a similar experiment...for some of the world’s most common diseases. References Zhang Y, Yin C, Zhang T, Li F, Yang W, Kaminski...

  21. How to Negotiate a Successful Lab Start Up Budget

    Type
    Blog Post
    Published
    Sept. 1, 2015, 2:30 p.m.
    ...on unnecessary materials, or even a loss of the offer all together. The problem is that the discussions...specialized equipment, try finding institutes that offer shared equipment that you can use at a lower cost...turned down a faculty position because none of the offering institutes were able to provide the resources ...

  22. Hot Plasmids: Spring 2025

    Type
    Blog Post
    Published
    June 3, 2025, 1:15 p.m.
    ...control of one of seven enhancers specific for different interneuron populations. The enhancers span all...(LAMP5) — as well as cholinergic (choline acetyltransferase, ChAT) neurons. The authors characterized ...effort to develop molecular tools for studying different brain cell types. Look for more of these interneuron-targeting...

  23. Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility

    Type
    Blog Post
    Published
    May 18, 2018, 7:32 p.m.
    ...of the program beneath it. You can jump to the different time stamps in the description to watch any sections...precisely. You can find a great discussion of different types of replication in Nosek and Errington 2017...scientific findings. This makes sense on many different levels - it’s hard to process large amounts of...

  24. Sequencing Options for CRISPR Genotyping

    Type
    Blog Post
    Published
    Oct. 4, 2016, 2:30 p.m.
    ...irrespective of in silico prediction. These techniques differ in important ways, but can also complement one ...sequencing (Hendel et al. 2015), a significant difference as CRISPR experiments move closer to the clinic...https://magazine.scienceforthepeople.org/.     References 1. Fu, Yanfang, et al. "High-frequency off-target...

  26. It’s CRISPR Week at Addgene!

    Type
    Blog Post
    Published
    May 13, 2019, 12:01 p.m.
    ...Twitter feed will be all about CRISPR. We’ll have a different theme for each day. Beginning today, we’ll start... Jennifer Tsang...

  27. We’re Going Viral This Week!

    Type
    Blog Post
    Published
    Oct. 14, 2019, 1 p.m.
    ...viral vector information. Each day, we’ll cover a different topic as shown in the graphic below. Follow the... Jennifer Tsang...

  29. New FLIS Plasmid (April Fools 2022)

    Type
    Blog Post
    Published
    April 1, 2022, 1:15 p.m.
    ... this new technology as helpful as we have!  References and Resources Additional resources on the Addgene...Addgene blog:  Flies 101: Catching flies in the lab References Abril, U.N.O., et. al. (2022) A plasmid-based...

  30. Congrats to the 2019 Deck The Lab Winners

    Type
    Blog Post
    Published
    Jan. 17, 2020, 3 p.m.
    ...MCDB 🎄🎅🎁🐸🐸🐸🐸 pic.twitter.com/2MPLawgNQl — Jennifer Landino (@jenelandino) December 3, 2019 Hope...  Jennifer Tsang...

  31. Twenty Years of Sharing Science!

    Type
    Blog Post
    Published
    Jan. 8, 2024, 3:25 p.m.
    ... to 764 scientists at 375 organizations in 21 different countries. It's safe to say we keep our shipping...science support to our efficient and easy tech transfer process to our website and educational resources...

  32. SciComm with the Experts at Science in the News Part 1

    Type
    Blog Post
    Published
    March 16, 2017, 1:06 p.m.
    ... the News hosts a podcast, seminars, a blog, conferences, and more for non-scientists. These programs ...Communicating with Non-Scientists How to Approach Different Audiences Listen to the SITN Podcast Many thanks...

  34. Bringing Renewable Energy to Addgene

    Type
    Blog Post
    Published
    Aug. 27, 2019, 1:08 p.m.
    ...higher percentage of renewable sources. This program offers options of between 14% - 100% electricity supplied...have chosen to participate at the highest level offered. As a result, we will be drawing 100% of our electricity...

  35. CRISPR Cheat Sheet

    Type
    Blog Post
    Published
    Jan. 27, 2021, 2 p.m.
    ... May 31, 2018 and last updated Jan 27, 2021 by Jennifer Tsang. We have some crisp new CRISPR material ...and destroy viruses that infect them. Commonly referred to as a bacterial immune system. Genome Editing...

  36. Depositor Week 2020 is Happening Now. Join us!

    Type
    Blog Post
    Published
    Nov. 16, 2020, 2:15 p.m.
    ...consistently contribute to our collection so that we can offer the scientific community the latest and greatest...  Jennifer Tsang...

  37. New Viral Vectors - Summer 2024

    Type
    Blog Post
    Published
    July 9, 2024, 1:15 p.m.
    ...vectors repository since March.  Addgene is now offering an AAV2 CAG-driven tTA expresser from the Gradinaru...PdCO, deposited by the Yizhar lab. Addgene is now offering AAV1 and AAV5 preparations of several variants...

  38. Performing In Vivo CRISPR Screens Using the FITS Approach

    Type
    Blog Post
    Published
    May 2, 2024, 1:15 p.m.
    ...activated T cells, precluding analyses of T cell differentiation beginning with naïve T cells. The Framework...improving CD8+ T cell responses to cancer.   References and resources Milling, L. E., Markson, S. C.,...

  39. Addgene’s Blog Gets a New Look!

    Type
    Blog Post
    Published
    Jan. 25, 2021, 5:54 p.m.
    ...website, the latest Addgene news, and awards that we offer there. Each topic category contains a overview ...  Jennifer Tsang...

  40. Editor's Choice, July 2016

    Type
    Blog Post
    Published
    Aug. 5, 2016, 3 p.m.
    ...benefits you can find in the post, may make Cpf1 the preferred CRISPR variant going forward.   Special Delivery...posts from July: Addgene at the ASM Microbe 2016 Conference Generating Mouse Models Using CRISPR/Cas9 Cpf1...
Showing: 641 - 680 of 866 results