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We narrowed to 839 results for: LEA

Showing: 641 - 680 of 839 results

  1. Developing a cold tolerant E. coli using specialized chaperones

    Type
    Blog Post
    Published
    Jan. 28, 2020, 2:35 p.m.
    ...consider them worth the trouble.  Enter ELSA: E. coli Learning Suboptimal Acclimatisation To resolve this impasse...potential collaborators and interested audiences. Please visit our team wiki or contact us at igemiiserb...

  2. 15 Years of Addgene: The Top 15 Plasmids

    Type
    Blog Post
    Published
    Jan. 8, 2019, 1:54 p.m.
    ...Cas9n (a D10A nickase mutant) to nick, rather than cleave DNA. Find pSpCas9n(BB)-2A-GFP (PX461). pSpCas9(...CRISPR collection Find plasmids for your research  Learn about our viral service ...

  3. AAV Q&A with Tim Miles

    Type
    Blog Post
    Published
    May 17, 2022, 1:15 p.m.
    ... tail vein injections but would like to avoid "leaking" into the brain, would you recommend using the ...for very high MOI on top of very high coverage. Please see this paper for more information about PHP.eB...

  7. GCE4All: Making Genetic Code Expansion Accessible

    Type
    Blog Post
    Published
    June 15, 2023, 1:15 p.m.
    ... canonical 20 amino acids. But in your quest to learn more about your protein of interest, you may find... both translation and fidelity of translation, leading to system-wide effects. And on top of that you ...

  8. Viral Vectors 101: The AAV Retrograde Serotype

    Type
    Blog Post
    Published
    April 27, 2023, 1:15 p.m.
    ...retrograde AAV work are not yet understood, one thing is clear: this application opens a lot of doors in neuroscience...Resources on the Addgene Blog Get Started with AAV Learn more about Lentivirus Find out More about Addgene's...

  9. Sharing is Caring: But How to Distribute Open Hardware?

    Type
    Blog Post
    Published
    Feb. 28, 2023, 2:15 p.m.
    ...community are particularly successful. Machine-learning denoising of raw data (CARE), segmentation of ...example of how sharing of data and molecular tools can lead to faster and more efficient science. Researchers...

  10. How Addgene Creates Content

    Type
    Blog Post
    Published
    Nov. 17, 2022, 2:15 p.m.
    ...involved yourself. You probably won’t be surprised to learn that Addgene’s mission statement extends beyond ...and our graphic designer to make sure things are clear, accurate, and in line with Addgene style.    ...

  11. 3 Challenges in Plant Synthetic Biology

    Type
    Blog Post
    Published
    July 22, 2014, 5:46 p.m.
    ...developed a genetic tool for plant engineering, please share it with Addgene to both build the plant synthetic...two Jack Russell Terriers. More SynBio Reading: Learn more about Revolution Bioengineering Follow RevBio...

  12. Plasmids 101: Yeast Vectors

    Type
    Blog Post
    Published
    Feb. 25, 2014, 7:11 p.m.
    ...selection markers.  The yeast origin of replication (Please note: This first section primarily pertains to ... used for color and growth selection if divalent lead ions are used in the growth media. ura4+ pyrimidine...

  14. Seven Tips for Using LinkedIn as a Scientist

    Type
    Blog Post
    Published
    Oct. 1, 2019, 1:21 p.m.
    ...to send invitations. Finally, if you don’t know clearly why you are inviting someone to connect, perhaps...give advice for your interviews.  Pro tip: You can learn a lot from the company LinkedIn page. This can include...

  15. Lighting Up Cell Signaling with Photoswitchable Kinases

    Type
    Blog Post
    Published
    July 25, 2017, 1:20 p.m.
    ...Treatment of the cells with a PP1/PP2A inhibitor leads to maintenance of phosphorylated MEK1/2 levels, ...PMC4097317. Additional Resources on the Addgene Blog Learn More about Photoactivatable Fluorescent Proteins...

  16. Viral Vectors 101: An Introduction to AAV

    Type
    Blog Post
    Published
    May 30, 2023, 1:15 p.m.
    ..., Luk H., et al. "Efficient serotype-dependent release of functional vector into the culture medium during...Our Primer for Beginners Read Our Lentivirus FAQ Learn about Viral Vector Elements Resources on Addgene.org...

  17. To Each HIS Own

    Type
    Blog Post
    Published
    March 2, 2023, 2:15 p.m.
    ...required the incorporation of a cleavage site and a post-purification cleavage step to remove the tag in order...

  18. Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening

    Type
    Blog Post
    Published
    April 12, 2016, 2:30 p.m.
    ...reprogramming, and beyond. For further details, please check out Wong et al 2016 and Wong et al 2015. ...PMCID: PMC4780610. Resources on the Addgene Blog Learn Best Practices for Designing Your gRNA Read How ...

  19. Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP

    Type
    Blog Post
    Published
    Nov. 24, 2015, 3:30 p.m.
    ...photoconvertible variants, it seems like GFP is always learning new things. Now, work from Connie Cepko’s lab ...easier. Advantages and other possibilities It’s clear that Cre-DOG and T-DOGGs open up many new possibilities...

  21. Mouse Modeling, Part 2: Breeding and Crossing Mice

    Type
    Blog Post
    Published
    Aug. 6, 2019, 12:55 p.m.
    ... engineered mice in your experiment. As you’ve learned in Part 1, there are many types of genetically ...about Cre-lox Read our Genome Engineering Guide Learn more in our CRIPSR guide ...

  22. Open Resources and Plasmid Tools For Studying C. elegans

    Type
    Blog Post
    Published
    July 18, 2019, 12:55 p.m.
    ...characterize the transcriptome. The first data release for CeNGEN is planned for August 15th, 2019. For...PMC4689222. Additional resources on the Addgene blog Learn more about SapTrap Read about CRISPR/Cas9 in C. ...

  23. Overcoming the Challenges of Lentiviral Production

    Type
    Blog Post
    Published
    Aug. 7, 2018, 12:31 p.m.
    ...different generations of lentiviral packaging systems please see Addgene’s Lentiviral Guide. While the third...cell line. For more tips on lentiviral titering please see our blog post. Between choosing the best lentiviral...

  27. Fluorescent Proteins 101: Introduction to FRET

    Type
    Blog Post
    Published
    June 27, 2017, 1:03 p.m.
    ... cells (Sustarsic and Kapanidis 2015), which may lead to monitoring of these processes in live animals...Addgene Blog Tips for using FRET in Your Experiments Learn about Fluorophore Targeting for FRET Studies FRET...

  28. Plasmids 101: Modular Cloning

    Type
    Blog Post
    Published
    March 12, 2024, 1:15 p.m.
    ...depend on many gRNAs delivered with a relevant Cas nuclease. MoClo can be used to assemble multiplexed gRNA...choosing a MoClo strategy and kit if you want to learn more about this cloning technique! Many thanks to...

  29. Adapting Toehold Switches to Detect Zika Virus

    Type
    Blog Post
    Published
    Aug. 30, 2016, 2:30 p.m.
    ...oligos to arrive. We also were careful to set up clear tasks for each member of the team to accomplish ...Lab Member and Sample 6 Cofounder Michael Koeris Learn How Minigenomes Are Being Used to Study Dangerous...

  30. Illuminating Epigenetics with A FRET Based Biosensor

    Type
    Blog Post
    Published
    Nov. 19, 2015, 3:30 p.m.
    ...behaviors, and a chronic feeling of hunger that can lead to excessive eating and obesity. What is fascinating... to visualize changes in gene expression and may lead to the development of a whole new range of methods...

  31. How-to: 5 Steps to a Great Panel Discussion

    Type
    Blog Post
    Published
    Oct. 22, 2014, 1:57 p.m.
    ... to admit to inviting interesting friends and colleagues to events for this purpose. If possible, make... more than 40 minutes for the panel discussion leaving 20 minutes for audience questions (30 if discussion...

  33. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    Published
    Sept. 24, 2024, 1:15 p.m.
    ...applications that don’t yet exist, but it wasn’t clear that this was even possible. There is a variety ...that MagLOV’s “astonishing” magnetoresponse was clearly visible whether expressed in E. coli, in mammalian...

  34. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    Published
    May 18, 2021, 1:15 p.m.
    ...page.  A new system uses light to deactivate photocleavable gRNAs within seconds. A CRISPR-based lineage...AAV5. AAV9 preps and more AAV1 versions will be released soon. Visualize axonal projections and putative...

  35. Cloning Mammalian Cells with the Agarose Method

    Type
    Blog Post
    Published
    Sept. 7, 2017, 12:17 p.m.
    ...appear as opaque dots (colonies should contain at least 50 cells and be well-separated. If the growth rate...to a 96-well assay plate. We generally freeze at least 2 ampules each of 4 clones with different levels...

  36. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type
    Blog Post
    Published
    May 4, 2017, 2:30 p.m.
    ...used to: Monitor dynamics of vesicle fusion and release from the plasma membrane by fusing various vesicle...PMC4831023. Additional Resources on the Addgene Blog Learn More About Fluorescent Protein Aggregation Catch...

  37. Viral Vectors 101: Voltage Indicators

    Type
    Blog Post
    Published
    Jan. 30, 2024, 2:15 p.m.
    ...cell level as they are in vitro. Factors like photobleaching, lack of red-shifted indicators, and brightness...selectively is especially essential. If you want to learn more about how viruses can be used for chemigenetics...

  39. CRISPR 101: Mammalian Expression Systems and Delivery Methods

    Type
    Blog Post
    Published
    Sept. 24, 2015, 2:30 p.m.
    ...Infects dividing and non-dividing cells. AAV is least toxic method for in vivo viral delivery. Cas9 ... Blog Check out our CRISPR Featured Topic Page Learn about CRISPR Delivery Using Ribonucleoproteins (...

  40. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    Published
    May 20, 2014, 2:06 p.m.
    ...temperature). Photostability: Fluorescent molecules gets bleached (i.e. lose the ability to emit light) after prolonged...imaging technologies to study gene expression, nuclear localization, and dynamics” Curr. Opin. Cell. Biol...
Showing: 641 - 680 of 839 results