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We narrowed to 406 results for: LARGE

Showing: 321 - 340 of 406 results
  1. Deep Dive: Statistical Tests (Comparisons)

    Type
    Blog Post
    Published
    March 7, 2023, 2:15 p.m.
    ... to reduce false positive rates and control for large numbers of comparisons. The Bonferroni does this...arranging all calculated p values from smallest to largest and ranking them (1 being the smallest value). ...calculates the cut off for significance by finding the largest p value where its rank divided by the total number...
  2. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    Published
    Jan. 6, 2015, 5:21 p.m.
    ...mechanism for sgRNA delivery and providing a means for large scale functional screens. For more information on... kinases Receptor tyrosine kinases (RTKs) are a large class of cell-surface receptors which play a critical...
  3. Important Considerations When Using AAVs

    Type
    Blog Post
    Published
    June 13, 2017, 2:30 p.m.
    ...preferential tropism for different brain regions is largely due the ability of different serotypes to infect...is fine and a researcher should aim to inject a larger amount of vector using a serotype with strong CNS... of adeno-associated virus serotypes: impact of larger genomes on infectivity and postentry steps." Journal...
  4. Sequencing Options for CRISPR Genotyping

    Type
    Blog Post
    Published
    Oct. 4, 2016, 2:30 p.m.
    ...require high read counts (fast multiplexing) Requires large cell population, senstive to time since cell fixing...; Pattanayak et al. 2013). This approach misses larger mismatches (e.g. six nucleotides) that may still...
  5. Antibodies 101: Flow Cytometry

    Type
    Blog Post
    Published
    July 20, 2021, 1:15 p.m.
    ...useful for intracellular staining where binding of large immune complexes may be hindered. Indirect staining...information about the relative size of the cell; larger cells produce more FSC. Light that scatters sideways...
  6. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    Published
    Sept. 10, 2020, 11:45 a.m.
    ...can help reduce off-target activity. Deleting a large region of the genome by removing the sequence between...
  7. Antibody Validation for Flow Cytometry

    Type
    Blog Post
    Published
    Sept. 10, 2024, 1:15 p.m.
    ...transfected with low efficacy. But since there can be large differences between endogenous expression and transfected... This can be particularly useful when designing larger multicolour panels, where different manufacturers...
  8. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    Published
    Oct. 12, 2023, 1:15 p.m.
    ...distributing widely throughout the organism to target large or diffuse biological structures. Of primary interest...transduction in Old World monkeys AAV.CAP-Mac is largely neurotropic in infant rhesus macaques and green...AAVs to transduce the same cell, can accommodate larger, modular genetic payloads. Since most systemic ...
  9. 15 Hot Plasmids from 2017

    Type
    Blog Post
    Published
    Jan. 9, 2018, 3:02 p.m.
    ...redshifted-emission spectrum compared to that of CyOFP1, a large stokes shift, long fluorescence lifetime, and a ...imaging. These variants were developed as part of a larger strategy to engineer improved red fluorescent proteins...
  10. Quantifying DNA? Here are Five DNA Quantification Methods to Consider

    Type
    Blog Post
    Published
    July 21, 2020, 1:15 p.m.
    ...electrophoresis. Smaller fragments migrate quicker than larger fragments. These fragments are quantified over ...that smaller fragments are quantified first before larger fragments. Figure 1: The quantification of...time. Smaller fragments migrate more quickly than larger fragments. Image from OpenWetWare. This method...
Showing: 321 - 340 of 406 results