We narrowed to 402 results for: LARGE

...mechanism for sgRNA delivery and providing a means for large scale functional screens. For more information on... kinases Receptor tyrosine kinases (RTKs) are a large class of cell-surface receptors which play a critical...

...Getting a vector into a particular cell type depends largely on the viral capsid, but specificity doesn’t end...

...their environment. These types of interactions to a large extent involve receptors expressed on the cell surface...

...preferential tropism for different brain regions is largely due the ability of different serotypes to infect...is fine and a researcher should aim to inject a larger amount of vector using a serotype with strong CNS... of adeno-associated virus serotypes: impact of larger genomes on infectivity and postentry steps." Journal...

...The best applications for dsDNA inserts include: large insertions or deletions, including selectable DNA...

...require high read counts (fast multiplexing) Requires large cell population, senstive to time since cell fixing...; Pattanayak et al. 2013). This approach misses larger mismatches (e.g. six nucleotides) that may still...

...useful for intracellular staining where binding of large immune complexes may be hindered. Indirect staining...information about the relative size of the cell; larger cells produce more FSC. Light that scatters sideways...

...are sensitive to distortions in helix shape (22). Large purine:purine mismatches and most smaller pyrimidine...

... at the same time pass over in silence his very large one” (http://oll.libertyfund.org/titles/galilei-...that the community of science supporters is much larger and stronger that I expected. Jennifer Stancill...

...) with 30-60 basepair homology arms to knock in large epitopes, such as GFP (Paix et al., 2014). A further...

...can help reduce off-target activity. Deleting a large region of the genome by removing the sequence between...

...-resolution imaging, they still do not emit the large number of photons achievable with photoswitchable...

...agencies to propose reforms to the H-1B visa system. Large firms and firms that work on projects with an international...

...transfected with low efficacy. But since there can be large differences between endogenous expression and transfected... This can be particularly useful when designing larger multicolour panels, where different manufacturers...

...distributing widely throughout the organism to target large or diffuse biological structures. Of primary interest...transduction in Old World monkeys AAV.CAP-Mac is largely neurotropic in infant rhesus macaques and green...AAVs to transduce the same cell, can accommodate larger, modular genetic payloads. Since most systemic ...

...redshifted-emission spectrum compared to that of CyOFP1, a large stokes shift, long fluorescence lifetime, and a ...imaging. These variants were developed as part of a larger strategy to engineer improved red fluorescent proteins...

...electrophoresis. Smaller fragments migrate quicker than larger fragments. These fragments are quantified over ...that smaller fragments are quantified first before larger fragments. Figure 1: The quantification of...time. Smaller fragments migrate more quickly than larger fragments. Image from OpenWetWare. This method...

...with a transfectable cell line. 2) They require larger expression vectors which are often more difficult...into viral vectors. Cas9 multiplexing vectors are larger because they require regulatory sequences to allow...individual promoters).  spCas9 and its gRNAs are also larger than their Cpf1 counterparts. Table 2. Cas9 Multiplexing...

...outlier, this story had us wondering: is there a larger trend here? Do papers associated with Addgene deposits...deposits are more highly cited, we went to one of the largest bibliographic databases available, Web of Science...

... and mediate biochemical processes. One of the largest and best characterized of these is the Src homology... sequencing, revealing that they bind to a much larger subset of protein motifs than previously thought...