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We narrowed to 749 results for: SOM

Showing: 321 - 340 of 749 results

  1. New CRISPR Web Resources and #12DaysofCRISPR Recap

    Type
    Blog Post
    Published
    Dec. 12, 2017, 3:13 p.m.
    ...We also updated some our most popular CRISPR blogs to keep them current. Here are some of the changes ...’ve also added four new plasmid pages that cover some of the hottest CRISPR tools. Base Edit: Base editing...

  2. FastCloning: A Newer, Simpler Cloning Technique

    Type
    Blog Post
    Published
    Nov. 1, 2016, 2:30 p.m.
    ...sequence alteration. Molecular cloning techniques are some of the most widely used techniques in the biological...efficiency and simpler procedures have been developed. Some of these include, TA cloning, ligation independent... step by step procedure of FastCloning, here are some tips for high efficiency cloning with this technique...

  4. Management for Scientists: Giving Feedback

    Type
    Blog Post
    Published
    Feb. 6, 2015, 2:46 p.m.
    ...good. Don’t pretend something went well when it didn’t… but don’t “punish” someone for a past infraction...positive feedback when it is merited. Surprisingly, some people find this hard so get used to it by making...

  5. Choosing Your Perfect Empty Backbone

    Type
    Blog Post
    Published
    Aug. 19, 2014, 3:39 p.m.
    ...described below some of the most frequently requested vectors in our repository and will discuss some of the features...approaches to studying YGOI's function in worms. Some of the more popular studies involve using a reporter...

  6. Meet the New Addgene Board Members!

    Type
    Blog Post
    Published
    April 28, 2020, 1:14 p.m.
    ...for Biomedical Research. Drug development was something Stegmeier has been interested in for a long time... genetics during graduate school at MIT. “I read some of the seminal papers on cell cycle discovery from... many interactions with Addgene over the years. “Some of the walls we’ve built create inefficiencies that...

  7. The Golden Stopwatch Award: Recognizing Facilitators of Research

    Type
    Blog Post
    Published
    Sept. 5, 2019, 1:06 p.m.
    ...Though they may seem like boilerplate drudgery to some scientists, MTAs are important—they guarantee certain...receive that legal agreement. Below, you will find some easy talking points for broaching this subject with...research institutions TTO: Technology Transfer Office. Some synonymous terms include: legal office, office of...

  8. Intro to Our Summer SciComm Series!

    Type
    Blog Post
    Published
    June 9, 2022, 1:15 p.m.
    ...best practices in the field, and even getting into some active science communication research.   This summer...as laypeople when it comes to molecular biology.  Some lay people have a high amount of knowledge or interest... the communication method, that determines if something is scientific or science communication. A Tweet...

  9. Three Tips for Preventing Viral Plasmid Recombination in Your Samples

    Type
    Blog Post
    Published
    Sept. 14, 2021, 1:15 p.m.
    ...common E. coli strains like DH5-alpha are recA-, some strains have been engineered to further reduce recombination...is important to not overgrow the plasmid at 37°C. Some researchers find that using a 30°C incubation temperature...for single colonies and testing multiple preps. In some cases, we suggest gel extraction to isolate the ...

  10. Viral Vectors 101: What is a Virus (and a Viral Vector)?

    Type
    Blog Post
    Published
    Aug. 18, 2023, 4:15 p.m.
    ...genome inside a protein shell known as a capsid, some also have a lipid bilayer surrounding the capsid...single particle creates many copies of itself, sometimes up to millions of new copies, which are released...the cell like baby spiders from an egg sac. (Okay, some enveloped viruses bud out, instead of lysing, but...

  11. Summer SciComm: Science Storytelling

    Type
    Blog Post
    Published
    Sept. 16, 2022, 1:15 p.m.
    ... science ever comes from an interview in which someone laughed and said, “Science is failing over and ...First, it should “hook” your audience in with something that sparks their interest and makes them want...the midst of all these storytelling devices, we sometimes forget the most important thing about science:...

  12. Creating accessible biology activities in schools with BioBits

    Type
    Blog Post
    Published
    Aug. 2, 2018, 12:56 p.m.
    ...in college that I really fell in love with it. Something about being able to actually hold all those seemingly...instructor at the college level, perhaps consider using some elements of BioBits to transition your undergraduate...educational uses. We believe that biology education is something that should be available to everyone, and hope...

  13. The Importance of High Titer for AAV Transductions

    Type
    Blog Post
    Published
    March 1, 2018, 2:11 p.m.
    ... that’s compatible with your experimental setup. Some experiments require higher gene expression and therefore...How do you achieve high titers in the first place? Some AAVs are known to be difficult to produce in high...ways you can optimize AAV production. We’ve listed some simple steps you can take below but you should read...

  15. Science Career Options

    Type
    Blog Post
    Published
    Feb. 23, 2017, 1:30 p.m.
    ...part time or full time) and what life is like for someone who currently in the position. Questions you’ll...well as work history can really show that you have some of the abilities listed above. Showing that you ...out our other career posts for more specifics on some careers and to find advice on crafting the perfect...

  16. Hot Plasmids - January 2023

    Type
    Blog Post
    Published
    Jan. 31, 2023, 2:15 p.m.
    ...through our hot plasmids articles. Think we missed something smokin'? You can pitch a Hot Plasmids section ...of our most commonly requested antibodies. But sometimes, one antibody per target just isn’t enough and...(recombinant) monoclonal. Another reason is that some antibodies may just not perform as well in one application...

  17. Plasmids 101: NGS Quality Control for Pooled Libraries

    Type
    Blog Post
    Published
    Oct. 26, 2017, 1:59 p.m.
    ... or even a million plasmids. These libraries are some of Addgene’s most exciting and versatile offerings...are a number of other analysis scripts available, some associated with specific libraries, but any analysis...amplification. I hope this post has helped clarify some of the important considerations when using, amplifying...

  18. Four Factors that Differentiate the Stem Cell Field

    Type
    Blog Post
    Published
    Sept. 28, 2016, 2:35 p.m.
    ...scientists. In my time at the meeting, I've made some observations regarding what makes this group of ...say that I'm glad we're so fast and affordable. Something in their world should be fast and affordable. .... 4. Stem cell scientists are generous Besides some of the scientists I've already mentioned, Addgene ...

  19. 5 Great Apps for Lab Life

    Type
    Blog Post
    Published
    Sept. 22, 2016, 2:30 p.m.
    ...skim the manual. Overall, it’s a great tool for someone starting cell culture work. 3. NEB Tools by New...properties of or draw an amino acid or peptide for some reason. It contains all twenty amino acids plus ...chain, except if their names have a B, J, X, or Z. Some alternative, similar apps: Findings Lab Notebook...

  20. Synthetic Photobiology: Optogenetics for E. coli

    Type
    Blog Post
    Published
    Sept. 8, 2015, 2:30 p.m.
    ...with one RR (ompR) encoded chromosomally. Leakiness was also an issue; some promoter activity persisted...Phytochromes are light-responsive systems found in some bacteria, but not E. coli. They fall under the heading...
Showing: 321 - 340 of 749 results