We narrowed to 762 results for: SOM

...and everything in between – now all you need are some of your favorite proteins purified to carry out ...used for both direct and indirect visualization. Some of these tags can also be used for protein purification...purification, just as affinity tags can sometimes be repurposed for visualization. However, due to the nature...

... labor-intensive project; scripts can be used in some cases, but much of this work has required manually...the graduate student down the hall who got it from some technician downstairs who got it from who knows ...submissions from scientists all over globe, with some surprising, and important, findings being shared...

...successfully takes up plasmid DNA. Among those that do, some now have plasmids that contain your gene of interest...selecting for the loss of a gene product - usually something toxic. This gene is found on the original plasmid...alleviates its toxic effect. Negative selection is sometimes called counterselection.  Toxin-antitoxin systems...

...either. But with a little Googling and some trial and error, I found some free design software that let me ...for drawing figures with lots of elements. It took some playing around to figure out all the settings and...

...sequence alteration. Molecular cloning techniques are some of the most widely used techniques in the biological...efficiency and simpler procedures have been developed. Some of these include, TA cloning, ligation independent... step by step procedure of FastCloning, here are some tips for high efficiency cloning with this technique...

...with the academic enterprise or that there weren’t some pretty hefty bumps during my graduate training. ...path to science communication This may come as somewhat of surprise to many of my high school friends....being an Outreach Scientist at Addgene has been awesome. The process of writing, editing, and publishing...

...good. Don’t pretend something went well when it didn’t… but don’t “punish” someone for a past infraction...positive feedback when it is merited. Surprisingly, some people find this hard so get used to it by making...

...described below some of the most frequently requested vectors in our repository and will discuss some of the features...approaches to studying YGOI's function in worms. Some of the more popular studies involve using a reporter...

...genome inside a protein shell known as a capsid, some also have a lipid bilayer surrounding the capsid...single particle creates many copies of itself, sometimes up to millions of new copies, which are released...the cell like baby spiders from an egg sac. (Okay, some enveloped viruses bud out, instead of lysing, but...

...for Biomedical Research. Drug development was something Stegmeier has been interested in for a long time... genetics during graduate school at MIT. “I read some of the seminal papers on cell cycle discovery from... many interactions with Addgene over the years. “Some of the walls we’ve built create inefficiencies that...

...best practices in the field, and even getting into some active science communication research.   This summer...as laypeople when it comes to molecular biology.  Some lay people have a high amount of knowledge or interest... the communication method, that determines if something is scientific or science communication. A Tweet...

...common E. coli strains like DH5-alpha are recA-, some strains have been engineered to further reduce recombination...is important to not overgrow the plasmid at 37°C. Some researchers find that using a 30°C incubation temperature...for single colonies and testing multiple preps. In some cases, we suggest gel extraction to isolate the ...

...Though they may seem like boilerplate drudgery to some scientists, MTAs are important—they guarantee certain...receive that legal agreement. Below, you will find some easy talking points for broaching this subject with...research institutions TTO: Technology Transfer Office. Some synonymous terms include: legal office, office of...

... science ever comes from an interview in which someone laughed and said, “Science is failing over and ...First, it should “hook” your audience in with something that sparks their interest and makes them want...the midst of all these storytelling devices, we sometimes forget the most important thing about science:...

...in college that I really fell in love with it. Something about being able to actually hold all those seemingly...instructor at the college level, perhaps consider using some elements of BioBits to transition your undergraduate...educational uses. We believe that biology education is something that should be available to everyone, and hope...

... that’s compatible with your experimental setup. Some experiments require higher gene expression and therefore...How do you achieve high titers in the first place? Some AAVs are known to be difficult to produce in high...ways you can optimize AAV production. We’ve listed some simple steps you can take below but you should read...

... or even a million plasmids. These libraries are some of Addgene’s most exciting and versatile offerings...are a number of other analysis scripts available, some associated with specific libraries, but any analysis...amplification. I hope this post has helped clarify some of the important considerations when using, amplifying...

...Read the first post here. Once you’ve identified some fly stocks and other reagents of interest, the next...next question to ask is what to do with them. In some cases, there might be a mutation that disrupts the...running with fly lines that are already available, sometimes you’ll have to make your own. In my next post,...

...part time or full time) and what life is like for someone who currently in the position. Questions you’ll...well as work history can really show that you have some of the abilities listed above. Showing that you ...out our other career posts for more specifics on some careers and to find advice on crafting the perfect...

...through our hot plasmids articles. Think we missed something smokin'? You can pitch a Hot Plasmids section ...of our most commonly requested antibodies. But sometimes, one antibody per target just isn’t enough and...(recombinant) monoclonal. Another reason is that some antibodies may just not perform as well in one application...