We narrowed to 363 results for: his

1. xCas9: Engineering a CRISPR Variant with PAM Flexibility

   Type

   Blog Post

   Published

   March 28, 2018, 6:52 p.m.

   ...Plasmids for Mammalian Systems Learn about CRISPR History ...

2. Viral Vectors 101: Voltage Indicators

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   Blog Post

   Published

   Jan. 30, 2024, 2:15 p.m.

   ...a genetic component. The VSD components have historically come from a variety of sources – voltage-gated...

3. Antibodies 101: Conventional vs Spectral Flow Cytometry

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   Blog Post

   Published

   Aug. 3, 2023, 1:15 p.m.

   ...spectral flow and can be thought of as a series of histograms flipped onto its side and compressed into a heat...

4. Starter guide to induced pluripotent stem cells (iPSCs) part 1:  A renaissance in regenerative medicine

   Type

   Blog Post

   Published

   Oct. 30, 2018, 1:04 p.m.

   ...PMCID: PMC2752493. 4. Bongso, A. and M. Richards, History and perspective of stem cell research. Best Pract...

5. Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards

   Type

   Blog Post

   Published

   May 22, 2015, 4:58 p.m.

   ...they should not be relied on exclusively because historical studies of pathogen research have conclusively...

6. AAVs in Retinal Gene Therapy

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   Blog Post

   Published

   July 31, 2018, 12:55 p.m.

   ... 10 more years to obtain FDA approval and make history in the form of Luxturna™. A better vector for ...

7. Plasmids 101: Secondary Nanobody Toolbox

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   Blog Post

   Published

   Feb. 27, 2018, 2:04 p.m.

   ...will bind to the column due to their N-terminal His14-bdNEDD8 tag. After several washes, this tag can ...

8. Getting to Know Expansion Microscopy

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   Blog Post

   Published

   June 20, 2023, 1:15 p.m.

   ... L (2022) Super-resolution microscopy: a brief history and new avenues. Philosophical Transactions Royal...

9. Plasmids 101: Choosing an Antibiotic Resistance Gene

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   Blog Post

   Published

   Oct. 10, 2023, 1 p.m.

   ...Ampicillin Ampicillin, often just called amp, is the historical king of the antibiotic resistance realm. Amp ...

10. Lentiviral Vector Uses and Overview

    Type

    Blog Post

    Published

    May 19, 2016, 2:30 p.m.

    ...applications of lentiviral vectors! Lentiviral vector history and components In the early 1990s, researchers ...

11. Cpf1: A New Tool for CRISPR Genome Editing

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    Blog Post

    Published

    Oct. 14, 2015, 2:30 p.m.

    ... off-target editing rates, determined using sophisticated genome-wide analysis. At many of its computationally...

12. To Codon Optimize or Not: That is the Question

    Type

    Blog Post

    Published

    Nov. 12, 2020, 2:15 p.m.

    ...are under expressed compared to others. Image from Hiss et al., 2017. Most commonly, scientists codon...

13. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type

    Blog Post

    Published

    Sept. 16, 2020, 1 p.m.

    ...post-injection. Livers from these mice were histologically normal and liver injury markers were not increased...

14. Finding nucleic acids with SHERLOCK and DETECTR

    Type

    Blog Post

    Published

    April 16, 2020, 1 p.m.

    ...as well as health-related single nucleotide polymorphisms (SNPs) from human saliva. SHERLOCKv2 provides...

15. Neuronal labeling with Spaghetti Monster

    Type

    Blog Post

    Published

    Aug. 14, 2018, 12:42 p.m.

    ...protein of interest (POI), that facilitate immunohistochemistry (IHC) experiments with tag-specific antibodies...

16. New Tools Enable CRISPRa for Neuroscience Applications

    Type

    Blog Post

    Published

    July 2, 2019, 12:51 p.m.

    ... visualizing neuronal differentiation by immunohistochemistry, it is not amenable to FACS.  Thus, the ...

17. Antibodies 101: Immunoprecipitation

    Type

    Blog Post

    Published

    Dec. 7, 2021, 2:15 p.m.

    ...technique to identify DNA binding proteins involved in histone modification. Similarly, RNA IP (RIP) can be used...

18. Tips for Writing a Good Cover Letter

    Type

    Blog Post

    Published

    May 21, 2024, 1:30 p.m.

    ...logistical, like briefly explaining a gap in your job history or sharing relocation plans. They can be skills-based...

19. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

    Type

    Blog Post

    Published

    Nov. 1, 2018, 12:35 p.m.

    ...PMID: 7768854. PubMed Central PMCID: PMC177048. Thisted, Thomas, and Kenn Gerdes. "Mechanism of post-segregational...

20. AAV Titers: Where Do They Come From And What Do They Mean?

    Type

    Blog Post

    Published

    Nov. 15, 2017, 3 p.m.

    ...Quantitative PCR (qPCR) Digital droplet PCR (ddPCR) Historically, a quantitative DNA hybridization method (DNA...