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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 809 results for: org

Showing: 321 - 340 of 809 results

  1. Hot Plasmids - October 2020

    Type
    Blog Post
    Published
    Oct. 13, 2020, 1:15 p.m.
    ...   Bensussen S, et al., iScience 2020. https://doi.org/10.1016/j.isci.2020.101330 Split-wrmScarlet, a..., 2020. Goudeau, J. bioRxiv 2020. https://doi.org/10.1101/2020.07.02.185249 Predicting cell cycle...proliferate. Eastman et al. Cell Reports 2020. https://doi.org/10.1016/j.celrep.2020.107804 The CRISPR corner...

  2. Antibodies 101: Choosing the Right Isotype

    Type
    Blog Post
    Published
    April 6, 2023, 3 p.m.
    ...Issue 8, pp. 977–983). SAGE Publications. https://doi.org/10.1177/002215549804600813 Erickson, P. A., Lewis... Cell Biology (pp. 283–310). Elsevier. https://doi.org/10.1016/s0091-679x(08)60255-1  Manning, C. F., ...e38313). Public Library of Science (PLoS). https://doi.org/10.1371/journal.pone.0038313 More resources from...

  3. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    Published
    March 23, 2021, 1:15 p.m.
    ...accurately.   Priode and Haynes, protocols.io. 2021. dx.doi.org/10.17504/protocols.io.brgcm3sw     New system... cells.      Tang et al., eLife 2020. https://doi.org/10.7554/eLife.61080     A new tool-kit for the...studies.    Duong et al., Genetics 2021. https://doi.org/10.1093/genetics/iyab006       The CRISPR corner...

  4. A New Generation of Adenine Base Editors Improves Editing in Primary Human Cells

    Type
    Blog Post
    Published
    May 7, 2020, 1:15 p.m.
    ...without DNA cleavage. Nature 551:464–471 . https://doi.org/10.1038/nature24644 Gaudelli NM, Lam DK, Rees HA...therapeutic application. Nature Biotechnology. https://doi.org/10.1038/s41587-020-0491-6 Additional resources ...CRISPR variant with PAM flexibility Resources on Addgene.org Get the basics of CRISPR in our CRISPR guide...

  5. Synthetic promoter AAVs for cell-type specific expression in retinal cells

    Type
    Blog Post
    Published
    March 12, 2020, 1:15 p.m.
    ...of our viral vector blog posts Resources at Addgene.org Find AAV plasmids Find ready-to-use AAV viral...humans. Nature Neuroscience, 22, 1345-1356. https://doi.org/10.1038/s41593-019-0431-2  Neff, E.P. An AAV library...macaque, and man. Lab Anim 48, 264 (2019). https://doi.org/10.1038/s41684-019-0380-0...

  6. Plasmids 101: Terminators and PolyA signals

    Type
    Blog Post
    Published
    March 31, 2016, 2:30 p.m.
    ...English Wikipedia, CC BY-SA 3.0, https://commons.wikimedia.org/w/index.php?curid=39322032   No terminator... at en.wikipedia, CC BY-SA 3.0, https://commons.wikimedia.org/w/index.php?curid=17554393   Use of ... all of our Plasmids 101 Posts Resources on Addgene.org Browse our Plasmid Reference Pages Just Starting...

  7. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

    Type
    Blog Post
    Published
    Dec. 1, 2015, 3:30 p.m.
    ...was pleased to be able to participate when the organizers of the Canton Nucleic Acids Forum (CNAF) 2015...variety (see slides from my talk below). Largely organized by Dmitry Samarsky, and a very welcoming team ...CRISPR Michael Terns from of the University of Georgia studies the CRISPR/Cas system as it relates to ...

  8. Starter guide to induced pluripotent stem cells (iPSCs) part 1:  A renaissance in regenerative medicine

    Type
    Blog Post
    Published
    Oct. 30, 2018, 1:04 p.m.
    ... produce all of the differentiated cells in an organism, including the embryonic and extraembryonic cell...quantities in specific “stem cell niches” found in many organs in the adult human body. These generate specialized...Differentiate the Stem Cell Field Resources on Addgene.org Visit Our Stem Cell Page Find Plasmids for Reprogramming...

  9. Google Forums Round Up: First Impressions of NgAgo

    Type
    Blog Post
    Published
    Aug. 4, 2016, 2:30 p.m.
    ... success of CRISPR-Cas9 across multiple model organisms (3) lies in the versatility of its delivery methods...in the comments section below or contact blog@addgene.org if you'd like to write a more detailed post ...Cpf1 Stacks Up to Cas9 and NgAgo Resources on Addgene.org Request NgAgo Find CRISPR Resources Catch Up...

  10. 3D Printing Meets CRISPR Cas9

    Type
    Blog Post
    Published
    Oct. 17, 2017, 12:55 p.m.
    ...is “…where molecules become real”.  We sometimes forget that the models we create are around 30 million...reticulum. And graduate students, momentarily forgetting that the physical model is based on their own...User by a First Time CRISPR User Resources on Addgene.org Visit Our CRISPR Guide Page CRISPR/Cas9 History...

  11. Quick Guide to Working with Drosophila Part 1: Getting Started with Flies

    Type
    Blog Post
    Published
    July 13, 2017, 2:30 p.m.
    ... of your gene’s functional biology in a living organism. This is the first post in a three-part series...book for newbies on how to work with flies. Flybase.org is absolutely essential. You can look up information...Drosophila Learn about Cre-Lox Resources on Addgene.org Find Drosophila Plamids Drosophila CRISPR Resources...

  12. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type
    Blog Post
    Published
    May 4, 2017, 2:30 p.m.
    ...there were a variety of ways to monitor cell, organelle, and protein localization. For instance, you might...microscope, fractionate samples to isolate particular organelles and their contents, or perform in situ hybridization...Choosing Your Fluorescent Protein Resources at Addgene.org Find Fluorescent Protein Timers Browse All Fluorescent...

  13. Transferable Skills: Negotiation

    Type
    Blog Post
    Published
    March 3, 2022, 2 p.m.
    ...with your colleagues within and outside of your organization. In my non-academic roles, some examples of ...development analyst, I negotiate with global organizations that partner with Addgene. What are the most...management Managing a team Leadership Resources on Addgene.org Check out our careers page Watch our career ...

  14. Tips for Using BLAST to Verify Plasmids

    Type
    Blog Post
    Published
    May 29, 2014, 1:29 p.m.
    ..., enter the common or scientific name into the Organism box. This small piece of information can significantly... of alternatives to BLAST: ExPASy - http://www.expasy.org/genomics/sequence_alignment EMBL-EBI - http...Snapgene-powered plasmid maps. Resources on Addgene.org Sequence Analysis of your Addgene Plasmid Addgene...

  15. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    Published
    May 20, 2014, 2:06 p.m.
    ...fluorescent when ectopically expressed in most organisms. Furthermore, fusing FPs to another protein usually...Additionally, many other FPs have been found in other organisms. Blue 424 - 467 nm Cyan 474 - 492 nm Green...Large Stokes Shift (LSS): Stokes shift (named after George G. Stokes) is the shift in wavelength from excitation...

  16. Better Dyeing Through Chemistry & Small Molecule Fluorophores

    Type
    Blog Post
    Published
    Sept. 8, 2016, 2:30 p.m.
    ...Worried about your budget? Email janeliafluor@janelia.hhmi.org to try out the JF dyes. Acknowledgements ...2015): 917-923. PubMed PMID: 26575238. 5. Crivat, Georgeta, and Justin W. Taraska. "Imaging proteins inside...Fluorescent Protein Should I Use? Resources on Addgene.org Generate Fluorescent Protein Fusions Request...

  17. CRISPR Antimicrobials

    Type
    Blog Post
    Published
    May 3, 2016, 2:30 p.m.
    ...their suppliers, and public health and medical organizations are working to help educate the public about...chance of an opportunistic infection like for organisms like C. difficile. In 2014, Citorik et al. and... out of the Genome  Additional Resources on Addgene.org Find CRISPR Plasmids for Your Research Read ...

  18. MXS Chaining

    Type
    Blog Post
    Published
    Feb. 7, 2017, 3:30 p.m.
    ...however, be appropriate for cloning genes from organisms with high CpG dinucleotide representation. The... you use MXS Chaining by emailing us at blog@addgene.org. Table 3: Chaining Based Cloning Methods ...101: Green Fluorescent Protein Resources on Addgene.org Browse our Fluorescent Protein Collection Find...

  19. Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!

    Type
    Blog Post
    Published
    Dec. 3, 2019, 11:38 a.m.
    ... (e.g. Retroviral) Species: Specify the source organism for a gene insert. (e.g. S. cerevisiae) Plasmid...functionally (e.g. Base Editing) and by target organism. Clicking through to RNA Editing brings us to ...Contact our scientific technical support at help@addgene.org and let us help.  Check back on Thursday for ...

  20. Negotiating Work and Life: How to Find the Joy

    Type
    Blog Post
    Published
    March 20, 2014, 3:04 p.m.
    ...grandfather’s 100th birthday, as your mom will never forgive you… #3 Honor the work day and keep it holy Keep...home which happens very, very fast! I will never forget the time my eight-year-old daughter vehemently ...temple, my kids’ school, and with professional organizations supporting women in science and career development...
Showing: 321 - 340 of 809 results