... of your gene’s functional biology in a living organism. This is the first post in a three-part series...book for newbies on how to work with flies. Flybase.org is absolutely essential. You can look up information...Drosophila Learn about Cre-Lox Resources on Addgene.org Find Drosophila Plamids Drosophila CRISPR Resources...

...there were a variety of ways to monitor cell, organelle, and protein localization. For instance, you might...microscope, fractionate samples to isolate particular organelles and their contents, or perform in situ hybridization...Choosing Your Fluorescent Protein Resources at Addgene.org Find Fluorescent Protein Timers Browse All Fluorescent...

... produce all of the differentiated cells in an organism, including the embryonic and extraembryonic cell...quantities in specific “stem cell niches” found in many organs in the adult human body. These generate specialized...Differentiate the Stem Cell Field Resources on Addgene.org Visit Our Stem Cell Page Find Plasmids for Reprogramming...

...is “…where molecules become real”.  We sometimes forget that the models we create are around 30 million...reticulum. And graduate students, momentarily forgetting that the physical model is based on their own...User by a First Time CRISPR User Resources on Addgene.org Visit Our CRISPR Guide Page CRISPR/Cas9 History...

... success of CRISPR-Cas9 across multiple model organisms (3) lies in the versatility of its delivery methods...in the comments section below or contact blog@addgene.org if you'd like to write a more detailed post ...Cpf1 Stacks Up to Cas9 and NgAgo Resources on Addgene.org Request NgAgo Find CRISPR Resources Catch Up...

..., enter the common or scientific name into the Organism box. This small piece of information can significantly... of alternatives to BLAST: ExPASy - http://www.expasy.org/genomics/sequence_alignment EMBL-EBI - http...Snapgene-powered plasmid maps. Resources on Addgene.org Sequence Analysis of your Addgene Plasmid Addgene...

...fluorescent when ectopically expressed in most organisms. Furthermore, fusing FPs to another protein usually...Additionally, many other FPs have been found in other organisms. Blue 424 - 467 nm Cyan 474 - 492 nm Green...Large Stokes Shift (LSS): Stokes shift (named after George G. Stokes) is the shift in wavelength from excitation...

... (e.g. Retroviral) Species: Specify the source organism for a gene insert. (e.g. S. cerevisiae) Plasmid...functionally (e.g. Base Editing) and by target organism. Clicking through to RNA Editing brings us to ...Contact our scientific technical support at help@addgene.org and let us help.  Check back on Thursday for ...

...however, be appropriate for cloning genes from organisms with high CpG dinucleotide representation. The... you use MXS Chaining by emailing us at blog@addgene.org. Table 3: Chaining Based Cloning Methods ...101: Green Fluorescent Protein Resources on Addgene.org Browse our Fluorescent Protein Collection Find...

...Worried about your budget? Email janeliafluor@janelia.hhmi.org to try out the JF dyes. Acknowledgements ...2015): 917-923. PubMed PMID: 26575238. 5. Crivat, Georgeta, and Justin W. Taraska. "Imaging proteins inside...Fluorescent Protein Should I Use? Resources on Addgene.org Generate Fluorescent Protein Fusions Request...

...their suppliers, and public health and medical organizations are working to help educate the public about...chance of an opportunistic infection like for organisms like C. difficile. In 2014, Citorik et al. and... out of the Genome  Additional Resources on Addgene.org Find CRISPR Plasmids for Your Research Read ...

...grandfather’s 100th birthday, as your mom will never forgive you… #3 Honor the work day and keep it holy Keep...home which happens very, very fast! I will never forget the time my eight-year-old daughter vehemently ...temple, my kids’ school, and with professional organizations supporting women in science and career development...

...plant tissue cultures.   C. roseus is not a model organism such as Arabidopsis or tobacco. Studying gene ...transgenic plants is a common problem in non-model organisms. Therefore, transient methods are usually the ...Read our Plasmids 101 blog posts Resources on Addgene.org Browse our Plant Plasmids and Resources page...

...a positive stir and was recognized by meeting organizers in their town hall.  Iterative improvement - ...Mentor-Singleton relationship. Some of the variability was organic, as some people need less assistance than others...take some leaps. Binning Singletons is its own organization, working to make scientific meetings more productive...

... salaries (which have been made by multiple organizations across academia). Increased postdoc wages, overtime... salaries as their website implies. These 5 organizations, according to the NSF data, account for 18% ...Good Management for Scientists Resources on Addgene.org Learn about Careers at Addgene ...

...region (approximately 500bp is best) and select the organism. It will then tell you the available gRNA targets... much more from our favourite mammalian model organisms in the near future.  Many thanks to our guest...Homology Directed Repair Additional Resources on Addgene.org Find Software Tools to Help Design You gRNA ...

...distinct subcellular environments, including the organelles that compose the secretory pathway, endocytic...formation, and proteolytic cleavage. FPs localized to organelles are equally susceptible to these non-native modifications... Lee Snapp. "Fluorescent proteins in cellular organelles: serious pitfalls and some solutions." DNA and...

...the observation of proteins in living cells and organisms. Both components of the chimera are encoded by...). However, these assays are qualitative. The Organized Smooth Endoplasmic Reticulum (OSER) assay (Costantini...Read our plasmids 101 blog series Resources on Addgene.org Browse the fluorescent protein collection Find...

... to living cells thus APEX is not suitable for organoid or in vivo studies (Che and Khavari, 2017). Find...many fusion proteins this would lead to protein/organelle aggregation. The Ting lab hypothesized that the...Efficient proximity labeling in living cells and organisms with TurboID." Nature biotechnology 36.9 (2018...

...extended-open integrins enable tissue morphogenesis, organ development, and the shaping of the extracellular...subunits (p. 2024.01.26.577394). bioRxiv. https://doi.org/10.1101/2024.01.26.577394 Jo, M. H., Li, J., Jaumouillé...mechanics. Nature Communications, 13(1), 7471. https://doi.org/10.1038/s41467-022-35173-w More resources from ...