...is then washed, and either eluted from a fixed surface or precipitated, dried, and then rehydrated. There...glass particles in glass milk, gel slices are first placed in a chaotropic salt sodium iodine (NaI) solution...with ddH2O and then ethanol. After regeneration, trace amounts of DNA (3 femtograms/ul) could still be ...cleaned with 1M phosphoric acid and re-used, but trace contamination could interfere with interpreting ...

...yellow derivatives) A206K Interferes with dimer interface (monomeric derivatives) K26R, Q80R, N146H, H231L...biosensors has been designed to detect a variety of intracellular conditions, including ion (such as Ca2+) concentrations...available. Others: We’ve really just scratched the surface of the potential applications for GFP. It has also...strand break repair, and to label pathogenic intracellular microbes to visualize host/pathogen interactions...

...express my concerns and request a replacement. I got my replacement in the end and the issue was resolved...less suitable for troubleshooting or issuing replacement products. In order to find good and helpful answers... in you – a technical support role is the right place to live it out, but ideally without asking too many...

...progress. You will also probably have a lot more face time with your bench mentor than your scientific...lot of details, especially when you are in a new place, learning something new. So how can you remember... walking through the protocol, by going to each space in order to double-check you remember where the ...Resources Resources on the Addgene blog Trading Spaces – Visiting Scientist Edition Mentoring for Scientists...

...target is present immediately upstream of a Protospacer Adjacent Motif (PAM), the sequence NGG.  If these... within the editing window (bases 4-8 of the protospacer). While this is a small window, this system can...system.  Fig. 1: Cytidine deamination takes place on the free strand of DNA and converts a C to U ...

...recombination; SSA, single strand annealing) took place and reconstituted the EGFP expression cassette. ...fragment containing the sgRNA target sequence can be placed in multi-cloning site (MCS) between EGFP fragments...extra precautions needed when dealing with RNA (e.g. face mask, and a dust free and specially cleaned work...mice that may have no mutation, costing money and space. As a rule, screen all of your pups unless you have...

...), the inner membrane space of mitochondria and chloroplasts, the extracellular milieu, and gram negative...should be taken into account when deciding where to place an FP within a fusion construct [1,2], i.e. positioning...mitochondria, lysosomes or they are secreted into the extracellular milieu. Many of the resident proteins within...

...is by no means exhaustive, but should be a good place to start when trying to pick your perfect promoter...promoter down regulation due to methylation or deacetylation. Ubc General expression mRNA Mammalian promoter...calmodulin. GAL1, 10 General expression mRNA Yeast adjacent, divergently transcribed promoters Inducible with...orientation to your gene Although this list is a great place to start, the tables above do not delve into the...

...The source of those conflicts can frequently be traced back to one of a few trouble spots:  Communication...here is pretty intuitive: You need to be willing to face conflict head-on. That doesn’t mean you have to ...and compromising. Though there may be a time and place for avoiding, competing, or accommodating, if you...knowledge to resolve conflicts. Conflict in the workplace is inevitable, but having who can help resolve...

...consuming and difficult. Finding volunteers who had space to shoot was another hurdle. As we slowly started...reopened. But there were still many restrictions in place, most importantly, masks.  Besides the muffled voices...strict safety protocols to keep Addgenies safe. Space was at a premium, so I had to figure out a solution... we were finally refining the process down to a place I could be happy with it. Cycle 5 - The Future! ...