We narrowed to 762 results for: SOM

...order aggregates. This property can be tolerated in some applications (e.g. labeling of cells or tracking...uploading it as a preprint, or documenting it in some other way. Besides the data, new plasmids and/or...

...light. Any fluorescent molecule will photobleach at some point. For live-cell imaging, it is desirable to...different ways. We describe a number of issues with some of the experimental designs used to make these measurements...

...strains ultimately perform the same function, there is some evidence that ccdB Survival™ can be more difficult...plasmid CcdB protein involves poisoning of DNA-topoisomerase II complexes.” J Mol Biol. 1992 Aug 5;226(3)...

... these options can be cumbersome, can lower on-target efficiency, and sometimes even increase off-target...

...Gate cloning into a modular cloning strategy. Sometimes referred to as MoClo, this strategy uses the Type...Christian M, Wang L, Zhang Y, Schmidt C, Baller JA, Somia NV, Bogdanove AJ, Voytas DF. Nucleic Acids Res. ...

...methods aren’t stealthy enough to gain access to some cells and tissues, such as human induced pluripotent...zygotes with CRISPR components. However, this sometimes requires intracellular injections into the cytoplasm...

...experiments.  In this blog post let's take a look at some of the other open resources discussed at the meeting... (Schwartz and Jorgensen, 2018).  These are just some examples of worm resources and tools openly shared...

...to select large sets (with overhangs that violate some of the traditional design rules) for successful ...deposited by NEB – and don’t forget to ask DAD for some help! References Potapov, V., Ong, J. L., Kucera...

...ability to be metabolized into clozapine (CLZ). While some scientists argue that these issues can be mitigated...These synthetic agonists can penetrate the brain and some, such as varenicline, are already approved for clinical...

...homo-oligomerization of a protein fused to CRY2, although some studies have shown that this clustering only happens...To overcome this issue, Shemesh et. al. created a soma-targeted derivative of the opsin CoChR, termied ...

... from Maya Kostman for the IGI.     Here are some highlights of the plasmids shared over the last ... plasmid sharing and more. As you’ve read above, some of these plasmids have been in the repository for...

...visual person. Why don’t you record a podcast or put some effort into making a video? Use your unique talent...annual meetings. 8) Training – Get a formal education Some people get into science communication by taking ...

... of reasons for this but the primary one is that some behavioral experiments require stimulation only ... in this primer on optogenetics we’ll delve into some of the important considerations when performing ...

... need to collect will depend on the serotype, as some rAAV serotypes are abundantly released into the ...10.1089/hum.2010.107 Zen, Z., Espinoza, Y., Bleu, T., Sommer, J. M., & Wright, J. F. (2004). Infectious titer...

...like BioLegend’s Fluorophore Guide Chart.  Pro tip! Some antibodies go by different names depending on your...specific value between the two channels. It is something your computer will do for you to better analyze...

...Planck Institute of Colloids and Interfaces. Here are some tips for cell culture that will hopefully help you...that cross-contamination can be identified. Even if some mammalian cells lines have similar morphologies,...

...thermostable periplasmic endonuclease that can sometimes co-purify with your plasmid DNA and lead to degradation...reached your lysate volume limits, the copy number of some origins of replication (e.g. p15A, ColE1) can be...

...how to create and use a standard curve and provide some general considerations for scientists planning to...of the unknown sample. The unknown should fall somewhere in the middle of the standard curve. If you do...

... to produce your own recombinant antibodies with some molecular biology and cell culture experience. Let...immunocytochemistry. It’s important to note that some antibodies are better suited for certain applications...

.... Additionally, these transposon also exhibit something called target immunity, whereby multiple proximal... R, Jiang Y, Yang J, Yang S (2020) Multicopy Chromosomal Integration Using CRISPR-Associated Transposases...

...locus, making them more flexible than Cas9/Cpf1. Some Cas13 enzymes prefer targets with a given single...Exchange) system (Abudayyeh et al., 2019). To avoid some of the inherent drawbacks for RNA editing of natural...

...would generally not be the same if weekends were somehow imposed on scientists. Figure 6: Model ...regions. Our investigation of model fit revealed some potential instabilities in the China data, so we...

...reach for that silica spin column, stop to consider some ways to purify DNA without a kit. DNA purification...precipitated, dried, and then rehydrated. There are some slight modifications depending on the type of DNA...

...many facts but the training of the mind to think something that cannot be learned from textbooks” (Brian,...Biochemistry and Molecular Biology Annual Meeting. Some of my students published their work identifying ...

...sounds simple, and it pretty much is, but there are some tips and tricks you can use to obtain optimum transformation... this process right the first time. I’ll lay out some guidelines here. A detailed transformation protocol...

... plasmid assembly (if primer design is correct). Some DNA sequences are not easy to amplify by PCR, so...Betaine (1 M) to the PCR mix. When PCR is not possible Some plasmids just won’t amplify! Examples of this are...

... We can all acknowledge that negative data is something every researcher experiences and that it often... J., Bahr-Davidson, J., Smith, P. H., Zhen, Z., Sommer, J. M., Pierce, G. F., & Qu, G. (2005). Identification...

...the article’s PubMed ID (PMID) ...or DOI Or get some keywords close enough... If in the past you have...be useful in an experiment, and want to see if someone has already created and deposited it with Addgene...

..., experiments can be performed in the absence of some viral proteins (compared to viral infection). Therefore...allow for efficient polymerase activity, whereas some ratios result in significantly reduced activity ...

...Researchers also discovered the modular format of some transcriptional activators: that the DNA binding...that use auxotrophic rescue genes as reporters and some that repress instead of activate transcription. ...