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  1. 10 Basic tips for mammalian cell culture

    Type
    Blog Post
    Published
    July 12, 2018, 1:09 p.m.
    ...Planck Institute of Colloids and Interfaces. Here are some tips for cell culture that will hopefully help you...that cross-contamination can be identified. Even if some mammalian cells lines have similar morphologies,...

  2. Viral Vectors 101: Producing Your rAAV

    Type
    Blog Post
    Published
    July 16, 2024, 1:15 p.m.
    ... need to collect will depend on the serotype, as some rAAV serotypes are abundantly released into the ...10.1089/hum.2010.107 Zen, Z., Espinoza, Y., Bleu, T., Sommer, J. M., & Wright, J. F. (2004). Infectious titer...

  3. Antibodies 101: Flow Compensation

    Type
    Blog Post
    Published
    May 16, 2024, 1:15 p.m.
    ...like BioLegend’s Fluorophore Guide Chart.  Pro tip! Some antibodies go by different names depending on your...specific value between the two channels. It is something your computer will do for you to better analyze...

  4. Plasmids 101: Optimizing Plasmid Yields

    Type
    Blog Post
    Published
    May 26, 2016, 2:30 p.m.
    ...thermostable periplasmic endonuclease that can sometimes co-purify with your plasmid DNA and lead to degradation...reached your lysate volume limits, the copy number of some origins of replication (e.g. p15A, ColE1) can be...

  5. Tips for arabidopsis transformation

    Type
    Blog Post
    Published
    Oct. 25, 2018, 1:23 p.m.
    ...sounds simple, and it pretty much is, but there are some tips and tricks you can use to obtain optimum transformation... this process right the first time. I’ll lay out some guidelines here. A detailed transformation protocol...

  6. Simplify Cloning with in vivo Assembly

    Type
    Blog Post
    Published
    Oct. 18, 2018, 12:37 p.m.
    ... plasmid assembly (if primer design is correct). Some DNA sequences are not easy to amplify by PCR, so...Betaine (1 M) to the PCR mix. When PCR is not possible Some plasmids just won’t amplify! Examples of this are...

  7. CRISPR 101: RNA Editing with Cas13

    Type
    Blog Post
    Published
    July 31, 2020, 12:30 p.m.
    ...locus, making them more flexible than Cas9/Cpf1. Some Cas13 enzymes prefer targets with a given single...Exchange) system (Abudayyeh et al., 2019). To avoid some of the inherent drawbacks for RNA editing of natural...

  9. DNA Purification Without a Kit

    Type
    Blog Post
    Published
    Feb. 11, 2020, 2:15 p.m.
    ...reach for that silica spin column, stop to consider some ways to purify DNA without a kit. DNA purification...precipitated, dried, and then rehydrated. There are some slight modifications depending on the type of DNA...

  11. Great Results Start with Great Standard Curves

    Type
    Blog Post
    Published
    Aug. 15, 2024, 1:15 p.m.
    ...how to create and use a standard curve and provide some general considerations for scientists planning to...of the unknown sample. The unknown should fall somewhere in the middle of the standard curve. If you do...

  12. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    Published
    Aug. 13, 2024, 1:15 p.m.
    ... to produce your own recombinant antibodies with some molecular biology and cell culture experience. Let...immunocytochemistry. It’s important to note that some antibodies are better suited for certain applications...

  13. Course-Based Undergraduate Research Experiences (CUREs)

    Type
    Blog Post
    Published
    Feb. 9, 2023, 2:15 p.m.
    ...many facts but the training of the mind to think something that cannot be learned from textbooks” (Brian,...Biochemistry and Molecular Biology Annual Meeting. Some of my students published their work identifying ...

  14. Negative Can Be Positive: Open AAV Data with Addgene

    Type
    Blog Post
    Published
    Sept. 6, 2022, 1:15 p.m.
    ... We can all acknowledge that negative data is something every researcher experiences and that it often... J., Bahr-Davidson, J., Smith, P. H., Zhen, Z., Sommer, J. M., Pierce, G. F., & Qu, G. (2005). Identification...

  15. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

    Type
    Blog Post
    Published
    Dec. 7, 2020, 2:53 p.m.
    .... Additionally, these transposon also exhibit something called target immunity, whereby multiple proximal... R, Jiang Y, Yang J, Yang S (2020) Multicopy Chromosomal Integration Using CRISPR-Associated Transposases...

  17. Tips for Screening with Yeast Two Hybrid Systems

    Type
    Blog Post
    Published
    Oct. 22, 2015, 2:30 p.m.
    ...Researchers also discovered the modular format of some transcriptional activators: that the DNA binding...that use auxotrophic rescue genes as reporters and some that repress instead of activate transcription. ...

  18. A Career in Grants: How To Become a Grant Professional

    Type
    Blog Post
    Published
    Aug. 20, 2015, 2:30 p.m.
    ... for an organization in a position that required some involvement in the grants process and managing programs...resources. 3. Get a mentor, find your people Find someone who is working or has worked as a Grant Professional...

  19. Using Video to Share Your Science: We Share Science

    Type
    Blog Post
    Published
    Nov. 3, 2016, 2:30 p.m.
    ...Creative titles and controversial topics can garner some attention, though in reality much of our research...about your research is not hard either. Here are some resources: ImpactStory, a website that helps researchers...

  20. AAV Titers: Where Do They Come From And What Do They Mean?

    Type
    Blog Post
    Published
    Nov. 15, 2017, 3 p.m.
    ...be affected by many experimental factors. To list some complicating factors for quantifying AAV: qPCR... or if the sample needs to be quantified again. Some of the issues with titrating by qPCR are addressed...

  21. Designing Your Chalk Talk for the Academic Job Interview

    Type
    Blog Post
    Published
    March 12, 2019, 1:37 p.m.
    ...talk is quite simple. It's you, a dry erase board, some markers (chalk is rare these days), and a room of..., if you cannot describe the likely outcome with some specifics, the search committee member cannot either...

  22. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type
    Blog Post
    Published
    Aug. 20, 2020, 2:30 p.m.
    ...compared to wild-type SpCas9, which also displays some cleavage with an NGA PAM. This variant may increase...potentially reversible since there is no genomic edit. Some Cas13 enzymes require a single base protospacer ...

  23. New CRISPR Tools: Cas7-11 and PASTE

    Type
    Blog Post
    Published
    Feb. 17, 2022, 2 p.m.
    ...variety of related bacteria with similar Cas systems. Some of these Cas systems have enzymes small enough to...could likely do it using Prime editing alongside some creative (and significant!) protein engineering....

  24. Hot Plasmids - November 2023

    Type
    Blog Post
    Published
    Nov. 14, 2023, 3:35 a.m.
    ...generate seven new prime editors, PE6a–g, that overcome some current prime limitations and enable impressive ...protein interactions or map a local proteome, but some applications have been challenging or inaccessible...

  25. Viral Production at Addgene

    Type
    Blog Post
    Published
    Oct. 24, 2023, 1:15 p.m.
    ...cultures are inspected for sterility. Transducibility Some viral vectors are tested in vitro and in vivo for...gene expression and/or function. These data are sometimes reported or posted on the material page for the...

  26. Viral Vectors 101: Preparing Pooled Libraries

    Type
    Blog Post
    Published
    July 13, 2023, 1:15 p.m.
    ...anyone who has worked with individual plasmids knows, some plasmids amplify well and others don’t. The size...s far better to verify via NGS now than to learn some of your plasmids didn’t amplify after your screen...

  27. Hot Plasmids Spring 2024

    Type
    Blog Post
    Published
    May 23, 2024, 1:15 p.m.
    ...Every few months we highlight some of the new plasmids, antibodies, and viral preps in our repository...number of cell types at different genomic loci — in some cases, over 20-fold higher editing than PEmax. The...

  28. Plasmids 101: Broad Host Range Plasmids

    Type
    Blog Post
    Published
    April 11, 2024, 1:15 p.m.
    ...plasmid can transfer to, replicate in, and persist in. Some plasmids have a narrow host range, while others ...fully understood, but scientists have identified some of the features that confer broad host range. These...

  29. Plasmids 101: Control Plasmids

    Type
    Blog Post
    Published
    April 9, 2015, 3:29 p.m.
    ...the independent variable you are testing and not some other unintended factor. Control plasmids are used...different days, etc, but outside constraints may impose some limitations and you should be cognizant of these...

  30. 3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells

    Type
    Blog Post
    Published
    Sept. 5, 2017, 1:58 p.m.
    ...them back to wild type), differentiate them into somatic cells affected by the disease and study what’s ...guide RNA binding site works well. However, since some guide RNAs may tolerate a mismatch in the binding...
Showing: 511 - 540 of 745 results