Multiplex Genome Editing with CRISPR-Cpf1
Type
Blog Post
Published
May 9, 2017, 2:12 p.m.
...with a transfectable cell line.
2) They require larger expression vectors which are often more difficult...into viral vectors. Cas9 multiplexing vectors are larger because they require regulatory sequences to allow...individual promoters). spCas9 and its gRNAs are also larger than their Cpf1 counterparts.
Table 2. Cas9 Multiplexing...