We narrowed to 791 results for: REP

... When we started in 2004, we were the plasmid repository. In 2016, we added viral vectors, and in 2022...ready-to-use antibodies. Now, we think of ourselves as a repository for DNA-based materials, including our popular... kits and libraries. This DNA-based materials repository contains 139,868 reagents from 5,821 labs in ... system for scientists using materials in our repository, from our customer and science support to our...

...really like? Gather data of course. Ask questions. Prepare by going sleuthing on the lab website, publication...well. What do they have to say about this lab’s reputation? If you are choosing a training lab, I strongly... meaningless tasks, removal of responsibility, repeated reminders of blunders, setting target up to fail...ensure that you are expanding your options and preparing for both academic and non-academic career paths...most papers Productive external collaborations Reputation for not being open PI helps lab members determine...

...to their utility in the health sector, phages represent a reservoir of carbohydrate binding proteins waiting...successfully identified using this method. Step 1: Prepare a random fragment library of phage DNA This first...with cloning large fragments. After fragmentation, repair sheared ends with T4 DNA polymerase (to create ...purification, but overnight at 30°C is a good start. Prepare a lysis solution containing your favourite bacterial...20°C (or -80°C) followed by 5 min at 37°C (each repeated 5 times) works well. Leaving the membrane overnight...

...graduate studies. Students also need more access to reputable, organized networks connecting international experts...the University of the Basque Country. Mentees represented a diverse group of individuals with technical...writing fellowships, giving oral presentations, and preparing for job interviews, expanded profesional networks...Spanish scientific community overseas. Official representatives from different Spanish universities and high...development of knowledge, experience, resources, and entrepreneurial visions thereby opening new frontiers for young...

...in a given AAV preparation. For detailed instructions and calculations on how to prepare a plasmid stock...Addgene has shipped thousands of AAV and lentiviral preps to scientists around the world. In the years since...improve both the yield and quality of the viral preps. For example, last year we published an article ...

...plasmid you didn't have to grow the bacteria and prep the plasmid before you begin your cloning experiment...Great news. A subset of our collection has been prepared as part of our new cloning grade DNA service and...plasmid without spending time and resources in preparative experiments.  We’re excited to begin offering...etc. and/or directly long term storage. Every preparation is quality controlled through next generation...

... and antibacterial drugs like penicillin and streptomycin. Mycoplasma can pass through filtration methods...the flasks (unless the source of culture is irreplaceable or highly expensive). Detecting mycoplasma contamination... but it’s a very good practice for prevention. Prepare a small quantity of media stock that would be used...culture flasks. In cases where the culture is irreplaceable or very expensive, there are some ways to rescue...made permanent damage to the cell culture it is irreparable. Some cells may die due to longer incubation ...

...one that was consistently welcoming. This bears repeating. Individuals that work in science policy are a..., deliver public comments, meet with local representatives, or even run for office yourself! Plus, at ...is science communication so an outsized way to prepare is to do lots of writing and presenting for non-scientist...science policy skills in graduate school via this report from the Scientific Citizenship Initiative.    ... learn more! Figure 2: The many ways to prepare for a science policy career. Image used with permission...

...Neuroscience Community blog to read reports from bloggers at the meeting – my report is currently in the works....benefit from depositing their constructs to the repository, and getting feedback from our community. It ... on a symposium discussing progress made in reprogramming astrocytes to functional neurons in vivo. And...

...favorite reagent, scientific technique, or plasmid repository at your next meeting.     If you’re reading...service provides ready-to-use AAV and lentiviral preps! Download the green AAV background.       Some ....         We also have ready-to-use lentiviral preps for COVID-19 research, pooled CRISPR lentiirus libraries...background.       Are none of those backgrounds representative of your favorite technique? Our lab-themed ...

... Untergasser Lab Preparation of chemically competent cells (protocol) Preparation of electrocompetent...Research to make competent cells because cells prepared using this kit can be transformed without heat...of electrocompetent cells (protocol)  Cells prepared using the Z-competent TM buffer can be easily ...

...after they were added to the repository, including beta-catenin reporter M50 Super 8x TOPFlash, scramble...2008 was marked by the influx of plasmids for reprogramming differentiated cells into pluripotent stem cells...now have 19 different ubiquitin plasmids in the repository. 100,000 Plasmids Shipped! - By 2010, Addgene...

...522 organizations in 41 countries enriched our repository with 15,020 new plasmids — a testament to the...viral vector serotypes, or hundreds of Data Hub reports detailing antibody experimental results. We also...options. Almost any AAV transfer plasmid in our repository can now be packaged on demand in five distinct...Antibody characterization is critical to enhance reproducibility in biomedical research. eLife, 13, e100211....

...Medical School. Addgene is proud to present a video reprint of the CRISPR article "Generation of Genomic Deletions...nucleases to harness non-homologous end joining repair (NHEJ) [1]. Our initial work was with TALENs, in...correlative biochemical characteristics and ectopic reporter assays. We have been interested in exploring the...by clustered regularly interspaced palindromic repeats (CRISPR)/Cas9 nuclease system in mammalian cells...

...bisporus.) Reporter constructs equivalent to site-STOP-site-myrRFP, with “site” representing the specific...cross-reactivity of these recombinases, each recombinase and reporter construct pair were tested for excision efficiency...cross-reactivity; however, the other recombinase/reporter pairs were inactive, even at very high recombinase...

... strand in the center of the protein. The repeat:anti-repeat duplex pokes out from the REC lobe and doubles....   The REC lobe binds tightly to the repeat:anti-repeat duplex of the guide RNA, confirming its sequence...looking at, let’s peek at unbound—“apo”—Cas9 from Streptococcus pyogenes.   Figure 2:  Crystal structure...

... can prepare you to present your work at conferences, help with manuscript and grant preparation, and ...science communication skills. Ultimately, my degree prepared me to be exactly the type of scientist I wanted...interview with them to learn how their master's degree prepared them for their career.  2. Are you looking for...

... can then be purified for subsequent library preparation and high-throughput sequencing. Improvements ...lab Very recently, the Henikoff lab published a preprint paper outlining improvements to the initial CUT... rather than to rely on nuclei extraction. They report that this change offers a significant improvement... in terms of initial steps of cell or tissue preparation.   All in all, CUT&RUN offers a great alternative...

...tasked with was creating and sustaining a biweekly report of our social media analytics, something that had... meant I was responsible for establishing a new report and routine across the marketing and content team...a fantastic spreadsheet template to build out a report on. I was able to understand what metrics would...larger content and marketing team, and I started preparing for my first official professional presentation...

...buy a box with the prepaid label and fill it with nitrile gloves, affix the prepaid label, and divert ... lab waste. Despite the fact that researchers represent a minority of the world’s population, we account... (in the U.S.). Their cooler boxes come with a prepaid return shipping label. To return the box, simply...