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Showing: 341 - 360 of 566 results

  1. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    Published
    Aug. 13, 2024, 1:15 p.m.
    ... you have multiple contaminating bands or a few strong ones, consider increasing the number of washes ...Schirrmann T. Expression of recombinant antibodies. Front Immunol. 2013 Jul 29;4:217. doi: 10.3389/fimmu.2013.00217...

  3. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

    Type
    Blog Post
    Published
    Dec. 7, 2020, 2:53 p.m.
    ...can occur in two different orientations, but is strongly biased towards T-RL, where the R end is proximal...https://doi.org/10.1126/science.abb2920 Vo PLH, Ronda C, Klompe SE, Chen EE, Acree C, Wang HH, Sternberg...

  4. The Importance of a Fun Workplace: Company Culture at Addgene

    Type
    Blog Post
    Published
    Sept. 3, 2015, 2:30 p.m.
    ...to this question.  We truly work in a unique environment where, in addition to furthering our mission ...). 3. Do something outside of your ordinary environment Team building events like Escape the Room or ...

  5. A Career in Grants: How To Become a Grant Professional

    Type
    Blog Post
    Published
    Aug. 20, 2015, 2:30 p.m.
    ...application. They have increased their demand for stronger budgets with clear budget narratives, logic models... a particular area, e.g. education, poverty, environment, early childhood development, or community development...

  7. Your Lentiviral Plasmid FAQs Answered

    Type
    Blog Post
    Published
    April 23, 2014, 1:08 p.m.
    ...Addgene Depositor (and lentiviral expert) Didier Trono's lab webpage explains how lentiviral particles are... of this article. Additional Resources: Didier Trono Lab: Lentivectors Toolbox Resources on the Addgene...

  8. Hot Plasmids - August 2020

    Type
    Blog Post
    Published
    Aug. 18, 2020, 1:15 p.m.
    ...- there's a simple method for that! By Michelle Cronin The benefits of nanobodies over conventional antibodies...optogenetic AAV for expression in forebrain GABA-ergic interneurons. These include mDlx-GFP, hDlx-FLEX-GFP, hDlx-Flex-dTomato...

  10. New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression

    Type
    Blog Post
    Published
    Jan. 24, 2023, 2:15 p.m.
    ...visible under both phase contrast microscopy and electron microscopy (Fig. 1b). Gas vesicle expression ...batch-sorted to isolate a polyclonal cell line with strong gas vesicle expression and robustness without chromatin-opening...

  11. Transferable Skills Guide: Career Planning Resources

    Type
    Blog Post
    Published
    Nov. 14, 2017, 2 p.m.
    ...school and beyond is to live in your lab, right? Wrong! While it is clearly a good idea to be diligent ...them for work. Addgene’s very own Joanne Kamens, a strong advocate for career planning, has written a great...

  12. Antibiotic Resistance: An Old Solution but a New Problem

    Type
    Blog Post
    Published
    Nov. 10, 2016, 3:30 p.m.
    ...bacterium picking up resistance genes from it’s environment (from DNA left behind after another bacterium...survival. Existing virtually ubiquitously in the environment, there is an intense amount of competition between...

  13. Healthcare Consulting: A Door to the Business of Life Sciences

    Type
    Blog Post
    Published
    Oct. 13, 2016, 2:30 p.m.
    ... the most rewarding parts of the job is being confronted with challenges that are crucial for the success...in a short amount of time can sometimes become cauldrons on the verge of boiling. On other occasions, clients...

  14. What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program

    Type
    Blog Post
    Published
    May 2, 2019, 12:41 p.m.
    ...development and evolution of histone genes in Strongylocentrotus purpuratus, the spiny purple sea urchin. ...demonstrate unusual parallel adaptation to disparate environments so can be used study the genetic basis of evolution...

  15. Lab to Office Culture Shock

    Type
    Blog Post
    Published
    Feb. 5, 2019, 1:04 p.m.
    ...it’s like to be properly onboarded. Most work environments outside of academia provide a formal training... until I was out of the lab and in an office environment. Ultimately, it will likely be these skills that...

  16. Hot Plasmids: Fall 2024

    Type
    Blog Post
    Published
    Nov. 21, 2024, 1:30 p.m.
    ...Epitope tag antibody collection grows By Ashley Waldron We are excited to highlight the Anti-V5 tag antibody...codon-optimized for C. elegans and included artificial introns to improve expression. When using these constructs...

  17. Tips for Writing a Good Cover Letter

    Type
    Blog Post
    Published
    May 21, 2024, 1:30 p.m.
    ...reproducible assay. That information can make you a much stronger candidate!  Additional uses The cover letter is...job with a lot of file management that asks for strong organizational skills. You might think, “I’d be...

  18. Tips for CRISPR Gene Editing in Mice

    Type
    Blog Post
    Published
    June 28, 2016, 10:59 a.m.
    ...however, if the microinjection was done as the pronuclei were fusing, you may have several mosaic mice....Daisuke, et al. "Generation of mutant mice by pronuclear injection of circular plasmid expressing Cas9...

  19. New CRISPR Tools: Cas7-11 and PASTE

    Type
    Blog Post
    Published
    Feb. 17, 2022, 2 p.m.
    ...CRISPR-based RNA editing - and further experiments strongly supported their initial findings.  The team originally...the chance of the insert being “dropped” in the wrong place, while increasing the number of potential ...

  20. To Codon Optimize or Not: That is the Question

    Type
    Blog Post
    Published
    Nov. 12, 2020, 2:15 p.m.
    ...from GenBank database (CUTG), that provides an electronic dataset for codon-usage of 257,468 genes across...scientists with the ability to selectively silence neurons in vivo (Slimko and Lester, 2003).  Addgene has...
Showing: 341 - 360 of 566 results