Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

We narrowed to 580 results for: RON

Showing: 341 - 360 of 580 results

  1. PITChing MMEJ as an Alternative Route for Gene Editing

    Type
    Blog Post
    Published
    Feb. 23, 2016, 3:30 p.m.
    ...repair. NHEJ joins the ends of a DSB in an error-prone fashion, with insertions and deletions common. In...plasmid integration; however, since NHEJ is error-prone, such a system is likely to introduce additional...

  2. Genome-wide Screening Using CRISPR

    Type
    Blog Post
    Published
    Aug. 18, 2015, 2:30 p.m.
    ... target DNA, which, when repaired through error-prone non-homologous end joining (NHEJ), usually results...of multiple gRNAs targeting the same gene can be strong evidence that a particular gene is actually important...

  4. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    Published
    Aug. 13, 2024, 1:15 p.m.
    ... you have multiple contaminating bands or a few strong ones, consider increasing the number of washes ...Schirrmann T. Expression of recombinant antibodies. Front Immunol. 2013 Jul 29;4:217. doi: 10.3389/fimmu.2013.00217...

  5. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

    Type
    Blog Post
    Published
    Dec. 7, 2020, 2:53 p.m.
    ...can occur in two different orientations, but is strongly biased towards T-RL, where the R end is proximal...https://doi.org/10.1126/science.abb2920 Vo PLH, Ronda C, Klompe SE, Chen EE, Acree C, Wang HH, Sternberg...

  6. Capturing a Moment with Marc Zimmer’s “The State of Science”

    Type
    Blog Post
    Published
    July 30, 2020, 1:15 p.m.
    ... of science, particularly public health and environmental science, by the Trump administration are difficulties... climate change, the degradation of the U.S. environment, and a decline in the competitiveness of U.S....

  8. An Introduction to Adenovirus

    Type
    Blog Post
    Published
    Oct. 3, 2023, 1:15 p.m.
    ...exposure, a lack of immunity, and then finally a synchronized exposure under these conditions once the measures...proteins and downstream cellular events, all recruit strong innate and adaptive immune responses that inactivate...

  9. Plasmids 101: Secondary Nanobody Toolbox

    Type
    Blog Post
    Published
    Feb. 27, 2018, 2:04 p.m.
    ...IgG antibody structures. Functional comparison Strong but Large: Advantages of Antibodies One of the ...are great indirect immunodetection tools is their strong signal strength. This signal strength is due in...

  10. Transferable Skills Guide: Identifying Your Transferable Skills

    Type
    Blog Post
    Published
    Jan. 11, 2018, 3:29 p.m.
    ...across most, if not all, industries and work environments. This is why they are called transferable skills...leader,” because you don’t see yourself  standing in front of a crowd and using your charisma to give people...

  11. Simplify Cloning with in vivo Assembly

    Type
    Blog Post
    Published
    Oct. 18, 2018, 12:37 p.m.
    ...Biology. He is interested in neuronal signal transduction, and uses electron cryo-microscopy to understand...

  13. The Importance of a Fun Workplace: Company Culture at Addgene

    Type
    Blog Post
    Published
    Sept. 3, 2015, 2:30 p.m.
    ...to this question.  We truly work in a unique environment where, in addition to furthering our mission ...). 3. Do something outside of your ordinary environment Team building events like Escape the Room or ...

  14. A Career in Grants: How To Become a Grant Professional

    Type
    Blog Post
    Published
    Aug. 20, 2015, 2:30 p.m.
    ...application. They have increased their demand for stronger budgets with clear budget narratives, logic models... a particular area, e.g. education, poverty, environment, early childhood development, or community development...

  15. Your Lentiviral Plasmid FAQs Answered

    Type
    Blog Post
    Published
    April 23, 2014, 1:08 p.m.
    ...Addgene Depositor (and lentiviral expert) Didier Trono's lab webpage explains how lentiviral particles are... of this article. Additional Resources: Didier Trono Lab: Lentivectors Toolbox Resources on the Addgene...

  16. Tips and Tricks for Using Golden Gate Modular Cloning (MoClo)

    Type
    Blog Post
    Published
    July 8, 2025, 1:15 p.m.
    ...Laboratory Golden Gate Cloning videos made by the Nicola Patron group, one of the developers of the MoClo system...extracting and sequencing the plasmid, or both! We strongly encourage you to sequence the entire insert region...

  17. Hot Plasmids: Fall 2024

    Type
    Blog Post
    Published
    Nov. 21, 2024, 1:30 p.m.
    ...Epitope tag antibody collection grows By Ashley Waldron We are excited to highlight the Anti-V5 tag antibody...codon-optimized for C. elegans and included artificial introns to improve expression. When using these constructs...

  18. To Codon Optimize or Not: That is the Question

    Type
    Blog Post
    Published
    Nov. 12, 2020, 2:15 p.m.
    ...from GenBank database (CUTG), that provides an electronic dataset for codon-usage of 257,468 genes across...scientists with the ability to selectively silence neurons in vivo (Slimko and Lester, 2003).  Addgene has...

  19. Hot Plasmids - August 2020

    Type
    Blog Post
    Published
    Aug. 18, 2020, 1:15 p.m.
    ...- there's a simple method for that! By Michelle Cronin The benefits of nanobodies over conventional antibodies...optogenetic AAV for expression in forebrain GABA-ergic interneurons. These include mDlx-GFP, hDlx-FLEX-GFP, hDlx-Flex-dTomato...
Showing: 341 - 360 of 580 results