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We narrowed to 749 results for: SOM

Showing: 341 - 360 of 749 results

  1. Choosing Your Fluorescent Proteins for Multi-Color Imaging

    Type
    Blog Post
    Published
    Oct. 9, 2014, 3 p.m.
    ...that the choice of fluorescent proteins requires some thought. The first step in choosing fluorescent...together but CFP and GFP (ex 488 nm / em 507 nm) show some crosstalk between the two fluorescent proteins. ...proteins or marking compartments. I hope this sheds some light on multicolor imaging with fluorescent proteins...

  2. Plasmids 101: How to Verify Your Plasmid Using a Restriction Digest Analysis

    Type
    Blog Post
    Published
    Aug. 28, 2014, 3:34 p.m.
    ... down the hall, it is always a good idea to take some time to confirm that you are working with the correct...strain such as JM110 or INV110. Avoid star activity. Some endonucleases (for example BamHI) are capable of...lanes look like your uncut lane then there is something wrong! Quantify your DNA. Loading too much DNA...

  3. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    Published
    Oct. 12, 2023, 1:15 p.m.
    ...used working definition of systemic capsids. After some consideration, we offer this definition: A systemic...systemic capsids are engineered, as well as highlight some considerations for their use. Then, we’ll provide...differences occur not just between mice and NHPs; some capsids show both different efficacy and tropism...2023 AAV-PHP.S* PNS, including ENS (broad): somatic and visceral sensory ganglia, type II spiral ganglion...E. E., Mahoney, J. T., Kojima, Y., Ding, Y., Somasundaram, S., Miller, J. A., Kalmbach, B. E., Radaelli...

  4. New and Upcoming Viral Vectors - May 2020

    Type
    Blog Post
    Published
    May 28, 2020, 1:15 p.m.
    ...inventory of ready-to-use viral vectors. Here are some of the AAV and lentiviral preps we have released...proteins at the beginning of April. Now, we've turned some of the most requested plasmids into lentiviral preps...targeting. While promoters and AAV serotypes can sometimes be used to target tissue types, recombinases allow...

  5. Plasmids 101: Transformation, Transduction, Bacterial Conjugation, and Transfection

    Type
    Blog Post
    Published
    June 25, 2019, 12:54 p.m.
    ...infected bacterium can either exist as transient extrachromosomal DNA, like a plasmid, or it can integrate into...factor (F-factor). The F-factor is found on an episome, a piece of DNA that can replicate on its own or... or be integrated within a bacterial chromosome and allows the donor bacterium to make a small “bridge...

  6. 15 Years of Addgene: The Top 15 Plasmids

    Type
    Blog Post
    Published
    Jan. 8, 2019, 1:54 p.m.
    ...things to come, we wanted to take a look back at some of the contributions from our depositors. Below ...puromycin cassette that makes it less efficient in some cell lines. pSpCas9(BB)-2A-Puro (PX459) V2.0 was...puromycin cassette that makes it less efficient in some cell lines so it may be best to go with pSpCas9(...

  7. Early Career Researcher Toolbox: Social Media for Scientists

    Type
    Blog Post
    Published
    Dec. 11, 2018, 12:46 p.m.
    ... and use of Twitter and LinkedIn probably had something to do with it. Five resources to help you get...post is designed with beginners in mind, there’s some great reminders for Twitter pros as well, like tip...each of the three social media platforms, there’s some statistics on what percent of scientists and engineers...

  8. Building and Validating Recombinant Antibodies: A Case Study

    Type
    Blog Post
    Published
    April 5, 2022, 1:20 p.m.
    ...closer look at the construction and validation of some of these tools created by the Trimmer Lab that will...of the correct clones, it was determined that for some constructs more than 90% of the colony PCR validated...aberrant IgG light chain transcript was expressed by some of the hybridomas and, as a result, subsequently...

  9. GCE4All: Making Genetic Code Expansion Accessible

    Type
    Blog Post
    Published
    June 15, 2023, 1:15 p.m.
    ... in some systems, rare codons and four-amino acid frameshift codons are also used. Note that some systems... which non-canonical amino acids (ncAAs, also sometimes called unnatural amino acids) are placed in a ...

  10. Viral Vectors 101: Viruses as Biological Tools

    Type
    Blog Post
    Published
    March 21, 2023, 1:15 p.m.
    ...hard-to-reach organs, such as the brain. Secondly, some viruses have a low immunogenicity which makes them...Mutagenesis Possible Adenovirus Remains Episomal (Little Risk of Insertional Mutagenesis) High ...Expression High Immunogenecity AAV Remains Episomal (Little Risk of Mutagenesis) Very Low Immunogenicity...

  11. A Deep Dive into BioBuilder

    Type
    Blog Post
    Published
    Oct. 11, 2017, 1:44 p.m.
    ...lessons to teach, and how — some choose to run full semester-long classes, some pick and choose a few lessons... they remember the moments when they discover something new for themselves and when they effectively contribute...

  12. Dos & Don'ts When Publishing a Scientific Manuscript

    Type
    Blog Post
    Published
    May 22, 2014, 4:02 p.m.
    ...subgroup of researchers in your area of expertise? Sometimes, the funders of your research may stipulate that...more reproducible and your results more valuable. Some journals, including F1000Research, also ask for ...published. In summary, it is a good idea to take some time and look into which journal would be best suited...

  13. Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number

    Type
    Blog Post
    Published
    Sept. 4, 2018, 12:53 p.m.
    ...number by introducing genes of interest into the chromosome. But this has its own set of problems. Rapidly...is expressed from a plasmid as opposed to the chromosome. Let’s take a look at how the stabilized promoters...levels of expression between a plasmid and the chromosome The team also showed that the levels of gene ...

  14. RANbodies: Reporter Nanobody Fusions

    Type
    Blog Post
    Published
    April 10, 2018, 12:56 p.m.
    ...reporters stain cultured cells and tissue sections, some reporters are better suited for particular applications... 10 HA or 10 MYC tags built into a GFP scaffold. Some GFP nanobodies  stain poorly when tagged with a ...secondary Enzymatic reactions on tissues can be cumbersome Tyramide reagents can be expensive Can only stain...

  15. Troubleshooting Your Plasmid Cloning Experiment

    Type
    Blog Post
    Published
    Sept. 24, 2019, 12:55 p.m.
    ...to troubleshoot the cloning experiment. Here are some tips that will help you with your cloning project... Before diving into the experimental work, spend some time outlining the construction of the plasmid and...plasmid troubleshooting guide, you hopefully have some new strategies to tackle that tricky cloning project...

  16. Addgene: The First Twenty Years

    Type
    Blog Post
    Published
    Dec. 19, 2024, 2:15 p.m.
    ..., we wanted to walk through some of our favorite milestones and share some thoughts from the community...began distributing ready-to-use viral vectors from some of our most popular lentiviral and AAV vectors, ...

  17. Antibodies 101: Fab Fragments

    Type
    Blog Post
    Published
    Sept. 5, 2024, 1:15 p.m.
    ...affinity reagents, and for good reason. However, sometimes you want a tool that is a little more compact ...applications and have similar advantages, though with some caveats.  First off, F(ab’)2 fragments are bivalent...less stable and have lower binding affinity than some of the larger fragments.  Think of antibody fragments...

  18. Viral Vectors 101: An Introduction to AAV

    Type
    Blog Post
    Published
    May 30, 2023, 1:15 p.m.
    ...infect (or not infect) different cell types with some specificity. There are a lot of AAV serotypes, many...expressed, viral cargo is usually quite stable and in some mammalian tissues has shown expression for several...host genome and therefore the transduced gene is episomal and does not divide and replicate in the same ...

  19. CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt

    Type
    Blog Post
    Published
    April 22, 2015, 2:06 p.m.
    ...carry CRISPR circuitry, it can slow their growth. Something has to be done about that.  The second problem...variation. The first one worked in each case. Sometimes in CRISPR people talk about off-target interactions...genetic designs of that scale could be created. Someone could take on an engineering project including ...

  20. Adeno Associated Virus (AAV) for Cell and Gene Therapy

    Type
    Blog Post
    Published
    Nov. 7, 2017, 1:59 p.m.
    ...to quickly identify underlying genetic causes of some human conditions, opening up new avenues for therapeutics...that are responsible for integration into host chromosomes, further minimizing potential activation of innate...example, prevalence of neutralizing antibodies against some AAV types, AAV1, 2, 3 and 5 are thought to be as...
Showing: 341 - 360 of 749 results