We narrowed to 816 results for: ache
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TypeBlog PostPublishedOct. 11, 2016, 2:30 p.m....desirable traits using traditional plant breeding approaches, these techniques are cumbersome, often requiring...highlight the specific modifications to CRISPR machinery that allow for the use of CRISPR in plants, and...in question. In plant cells, gRNA expression is achieved by placing the gRNA downstream of a plant-specific...Glycol (PEG), although the application of this approach is limited to protoplast cells (plant cells whose...
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Plasmids 101: Expressing shRNAs for RNA Interference
TypeBlog PostPublishedJune 17, 2025, 1:15 p.m....therapeutic settings. Silencing of a target gene is achieved through the delivery of a double-stranded RNA ... plasmids, shRNAs hijack the endogenous miRNA machinery for processing (Figure 1). The shRNA sequence ...the bound miRISC can simply block translation machinery. The composition of the RISC varies, but at the... Silencing Complex: a versatile gene-silencing machine. Journal of Biological Chemistry, 284(27), 17897... -
Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection
TypeBlog PostPublishedApril 9, 2019, 1:13 p.m....tag. Many thanks to our guest blogger, Joachim Goedhart! Joachim Goedhart is an assistant professor at ...This post was contributed by guest blogger Joachim Goedart, an assistant professor at the Section of ...fluorescent probes. You can follow him on twitter: @joachimgoedhart. References Adjobo-Hermans, Merel JW, et ... -
Evolution of Lab Techniques
TypeBlog PostPublishedJune 21, 2016, 2:30 p.m....allowing for the study of their function. This approach to studying DNA sequences helped researchers at...Researchers perform four different reactions, one for each radiolabelled ddNTP, and then run these reactions...generally considered first generation DNA sequencing machines (9). Today, similar technologies, sometimes referred...older technologies nanopore-based DNA sequencing machines are relatively small meaning that DNA sequencing... -
Hot Plasmids: Spring 2025
TypeBlog PostPublishedJune 3, 2025, 1:15 p.m....small number of glutamate molecules are released in each synaptic event and they are rapidly taken up by ...for two-photon (2P) imaging and loose-seal, cell-attached recordings in primary visual cortex (V1) layer... is scarless, unlike previous integrase-based approaches. Find STITCHR plasmids here! Fell, C. W., Villiger...'s important to know what proteins interact with each other and where those interactions occur in the ... -
Is this the right place for me? 8 tactics for choosing a lab
TypeBlog PostPublishedOct. 2, 2018, 12:56 p.m....role model Make sure the mentors you choose are teaching the stuff you want to know and display character...members speak disrespectfully of supervisors and/or each other. You are not allowed to speak to lab members... a mentor that sees you only as a paper making machine. Choose one that is a true mentor, supporting career...collaboration? If you are considering a career at a small, teaching college you might want to consider less expensive... -
How to Design Your gRNA for CRISPR Genome Editing
TypeBlog PostPublishedSept. 24, 2020, 1:15 p.m....blog post will discuss differences between these approaches, and provide updates on how best to design gRNAs...should not be undertaken lightly! Indeed, truly achieving the gold standard requires not one but two rounds... using knockout or transcriptional modulation approaches. Avoiding off-target effects The off-target ...Guell M, Byrne S, Yang JL, De Los Angeles A, Mali P, Aach J, Kim-Kiselak C, Briggs AW, Rios X, Huang P-Y, ... -
Easi-CRISPR: Generating Knock-In and Conditional Mouse Models
TypeBlog PostPublishedApril 5, 2018, 12:42 p.m....homology on each side. For optimal repair, place the loxP sites precisely at the cut site for each guide. ...When delivering the ssDNA template and CRISPR machinery, Quadros et al. recommend ribonucleoprotein (RNP...strands, as well as guides facing towards or away from each other. Figure 4: How to design a conditional... -
Plasmids 101: Restriction Cloning
TypeBlog PostPublishedFeb. 18, 2016, 3:42 p.m....it is also a good idea to run an uncut sample of each plasmid to help with troubleshooting if your digests...approximately 1:3. Since the number of base pairs for each varies, it is difficult to calculate this based ... alone. One method is to conduct 2 ligations for each plasmid you are trying to create, with varying ratios...2), or roughly equivalent numbers of colonies on each plate (unsuccessful 3). If you have a high ... -
Introducing the Addgene Antibodies 101 eBook!
TypeBlog PostPublishedOct. 29, 2024, 1:15 p.m....eBook is designed to be a helpful desktop reference. Each chapter brings together all of our 101 blog posts...collection useful. And, as always, don’t hesitate to reach out to [email protected] with any thoughts, questions... Rachel Leeson... -
Typing CRISPR Systems
TypeBlog PostPublishedMarch 18, 2025, 1:15 p.m....to arrive at this hierarchy, the main aspects of each class and type are easy to understand. Classes are...involved in plasmid competition or can hijack machinery from other CRISPR systems to carry out various...further broken down into type II, type V, and type VI, each relying on a unique Cas effector — Cas9, Cas12, ...basic diversity and the surface-level function of each type could lead you to new directions in your research... -
Plasmids 101: Modular Cloning Applications and Kits
TypeBlog PostPublishedMay 14, 2024, 1:15 p.m....discuss. If you can think it, MoClo might help you achieve it! MoClo applications and specialized tools Synthetic...applications in bacteria or cell-free systems. The approach is particularly useful for producing drug precursors...a broad range of parts, including copy number machinery, chromosomal integration modules, and many more...can dream up an experiment, MoClo can help you achieve it! More recently, a CRISPR MoClo kit termed Fragmid... -
Twelve Amazing Guest Blog Contributors!
TypeBlog PostPublishedNov. 26, 2024, 2:15 p.m....Performing In Vivo CRISPR Screens Using the FITS Approach Caitlin Faulds, who introduced the very first...Applications and Kits We are very grateful for each and every contributor to the blog. Thank you! More... Rachel Leeson... -
New Podcast Segment: Hot Plasmids
TypeBlog PostPublishedMay 17, 2017, 3:37 p.m....generally publish 5-10 Hot Plasmid articles with each of our newsletters. You can find all of these articles...you haven’t got time to sit down and read through each one, plug in some headphones to learn more about...through this podcast segment. Below, we break down each of the Hot Plasmids segments into its own (highly... -
#DeckTheLab with Tinsel and Test Tubes, Fa La La La La La La La La
TypeBlog PostPublishedDec. 2, 2021, 2:15 p.m....finally December, and here at Addgene HQ in Massachusetts that means it’s time for near-freezing temperatures...This edition of the contest is our traditional approach. You might decorate your lab in pipette tip snowflakes...home editions. We will select up to 3 winners for each edition and the winners will be revealed on Twitter... -
Streaking for Single Colonies: The Streak Plate Challenge
TypeBlog PostPublishedMarch 2, 2021, 2:15 p.m....tasked the students to separate out colonies from each individual strain using the streak plate technique...University of Science and Technology, has been teaching the microbiology lab every year for over 20 years...strains are easily amenable to other experiments to teach other aspects of molecular biology. “What I'd like... -
Ghanaian Researchers to Receive Plasmid Awards from Addgene and Seeding Labs
TypeBlog PostPublishedJune 15, 2016, 1:30 p.m....these plasmids have a heavy focus on teaching. He hopes to teach his molecular biology students about ...Working on Rare Diseases Read About Some of Our Outreach Efforts in China Additional Resources on Addgene.org... -
Editor's Choice, September 2016
TypeBlog PostPublishedOct. 7, 2016, 4 p.m.... is my pleasure to announce that we once again reached new heights on the Addgene blog: we surpassed 60,000...Proteins," was contributed by guest blogger Jessica Sacher from the University of Alberta and describes a ...currently on my way back from a very successful Outreach Trip in San Francisco and would like to give my... -
Our Most Popular Blog Posts from 2019
TypeBlog PostPublishedDec. 31, 2019, 2:26 p.m....Making the Right Connection by Joachim Goedhart Guest blogger Joachim Goedhart shares some strategies...molecule, are often named after vegetables; Broccoli, Spinach, and Corn, for example. Learn more about the aptamer... -
CRISPR Kinome Libraries Available: Pooled and Individual Plasmid Formats
TypeBlog PostPublishedSept. 9, 2016, 2:43 p.m.... huge, there are times when a more incremental approach is prudent. What's in the CRISPR kinome library...available from Addgene as pools provided in two sets, each with 4 gRNAs per kinase, so screens can be performed...gRNA, for use with cells that already have Cas9). Each library consists of approximately 3,000 gRNAs, as...