We narrowed to 365 results for: his

1. To Codon Optimize or Not: That is the Question

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   Blog Post

   Published

   Nov. 12, 2020, 2:15 p.m.

   ...are under expressed compared to others. Image from Hiss et al., 2017. Most commonly, scientists codon...

2. Antibodies 101: Immunoprecipitation

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   Blog Post

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   Dec. 7, 2021, 2:15 p.m.

   ...technique to identify DNA binding proteins involved in histone modification. Similarly, RNA IP (RIP) can be used...

3. Mesothelioma - Causes, Symptoms, and Treatment

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   Blog Post

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   Dec. 13, 2016, 3:30 p.m.

   ...brought a lot of promise to a field that has historically been full of bad news and dreadful prognoses...

4. Getting the Most from Your Lentiviral Transduction

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   Blog Post

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   April 7, 2016, 2:30 p.m.

   ...discarding and thawing a fresh vial. In many labs, the history of the cells in storage may be a bit dubious due...

5. Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

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   Blog Post

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   Sept. 1, 2020, 1:15 p.m.

   ...key to fitting large genes into AAV First, some history. In 2008, the Auricchio lab decided to solve the...

6. An Integrin Antibody Toolkit from IPI

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   Blog Post

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   March 28, 2024, 1:15 p.m.

   ...will also work in immunoprecipitation and immunohistochemistry. Table 1: IPI antibodies  Antibody ...

7. Antibodies 101: Introduction to Immunofluorescence

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   Blog Post

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   Nov. 16, 2021, 2:15 p.m.

   ...with IF are immunocytochemistry (ICC) or immunohistochemistry (IHC). All three of these terms refer to...

8. 10 Steps to a Perfect Science Talk

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   Blog Post

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   Aug. 23, 2016, 2:30 p.m.

   ... like they’re sitting through a pre-recorded lecture.This takes practice (see above) and a deep familiarity...

9. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

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   Blog Post

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   May 10, 2016, 2:30 p.m.

   ...HIV-infected reservoir, researchers have previously used histone deacetylase (HDAC) inhibitors to increase transcription...

10. Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods

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    Blog Post

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    March 1, 2016, 3:30 p.m.

    ... 1. Gibson DG, Young L, Chuang RY, Venter JC, Hutchison CA 3rd, Smith HO. Enzymatic assembly of DNA molecules...

11. Treating Muscular Dystrophy with CRISPR Gene Editing

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    Blog Post

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    Jan. 26, 2016, 3:30 p.m.

    ... blot, at <10% of the wild-type level. Muscle histology is improved, with vastly reduced inflammation ...

12. Plasmids 101: Repressible Promoters

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    Blog Post

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    Dec. 20, 2022, 2:15 p.m.

    ... GAL80 under different promoters and create sophisticated patterns of UAS-driven gene expression. To further...

13. Antibodies 101: The Basics of Western Blotting

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    Blog Post

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    March 8, 2021, 2:41 p.m.

    ...and references References Aydin S (2015) A short history, principles, and types of ELISA, and our laboratory...

14. Finding Your Science Policy Path

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    Blog Post

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    Sept. 22, 2020, 1:15 p.m.

    ...move into science policy. Read broadly about the historical roots of science policy to connect to your why...

15. Deep Dive: Statistical Tests (Comparisons)

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    Blog Post

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    March 7, 2023, 2:15 p.m.

    ...distribution you are working with, graphing data with a histogram is sometimes sufficient to determine normality...

16. FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution

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    Blog Post

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    Oct. 17, 2019, 12:28 p.m.

    ...Despite its relatively recent development, the history of optogenetics begins almost 50 years ago with...

17. Typing CRISPR Systems

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    Blog Post

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    March 18, 2025, 1:15 p.m.

    ...Alfonse, L. E., Carte, J. M., Keston-Smith, E., Sothiselvam, S., Garrity, A. J., Chong, S., Makarova, K. ...

18. Antibodies 101: Flow Cytometry

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    Blog Post

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    July 20, 2021, 1:15 p.m.

    ... then gate the B cell population and create a histogram of CD19-containing cells versus total cell number...

19. Cancer and the Immune System: Deciphering the Relationship

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    Blog Post

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    March 14, 2017, 2:30 p.m.

    ... downregulate the expression of the major histocompatibility complex (MHC) class I proteins on their surfaces...

20. CRISPR 101: Cytosine Transversion Editors

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    Blog Post

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    March 25, 2025, 1:15 p.m.

    ...the SOX2 activation domain (SAD), which recruits histone acetyltransferase to open chromatin, improved editing...