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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 809 results for: org

Showing: 341 - 360 of 809 results

  1. Plasmids 101: Shuttle Vectors

    Type
    Blog Post
    Published
    May 20, 2025, 1:15 p.m.
    ...entirely. Auxotrophic selection relies on the host organism being unable to synthesize a nutrient required... to design your vector to be functional in the organism your experiment takes place in. In many cases,...Plasmids 101: The Promoter Region Resources on Addgene.org Collection of empty plasmid backbones ...

  2. Changing Labor Laws Bring Increased Postdoc Wages

    Type
    Blog Post
    Published
    Oct. 25, 2016, 2:30 p.m.
    ... salaries (which have been made by multiple organizations across academia). Increased postdoc wages, overtime... salaries as their website implies. These 5 organizations, according to the NSF data, account for 18% ...Good Management for Scientists Resources on Addgene.org Learn about Careers at Addgene ...

  3. Tips for CRISPR Gene Editing in Mice

    Type
    Blog Post
    Published
    June 28, 2016, 10:59 a.m.
    ...region (approximately 500bp is best) and select the organism. It will then tell you the available gRNA targets... much more from our favourite mammalian model organisms in the near future.  Many thanks to our guest...Homology Directed Repair Additional Resources on Addgene.org Find Software Tools to Help Design You gRNA ...

  4. Save Time with Transient Plant Leaf Transformations

    Type
    Blog Post
    Published
    July 25, 2019, 12:03 p.m.
    ...plant tissue cultures.   C. roseus is not a model organism such as Arabidopsis or tobacco. Studying gene ...transgenic plants is a common problem in non-model organisms. Therefore, transient methods are usually the ...Read our Plasmids 101 blog posts Resources on Addgene.org Browse our Plant Plasmids and Resources page...

  5. Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone

    Type
    Blog Post
    Published
    Feb. 18, 2020, 2:15 p.m.
    ...a positive stir and was recognized by meeting organizers in their town hall.  Iterative improvement - ...Mentor-Singleton relationship. Some of the variability was organic, as some people need less assistance than others...take some leaps. Binning Singletons is its own organization, working to make scientific meetings more productive...

  6. Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

    Type
    Blog Post
    Published
    Oct. 27, 2015, 3 p.m.
    ...distinct subcellular environments, including the organelles that compose the secretory pathway, endocytic...formation, and proteolytic cleavage. FPs localized to organelles are equally susceptible to these non-native modifications... Lee Snapp. "Fluorescent proteins in cellular organelles: serious pitfalls and some solutions." DNA and...

  7. Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

    Type
    Blog Post
    Published
    April 9, 2019, 1:13 p.m.
    ...the observation of proteins in living cells and organisms. Both components of the chimera are encoded by...). However, these assays are qualitative. The Organized Smooth Endoplasmic Reticulum (OSER) assay (Costantini...Read our plasmids 101 blog series Resources on Addgene.org Browse the fluorescent protein collection Find...

  8. Multiple Plasmids at a Low Price: Kits from Addgene

    Type
    Blog Post
    Published
    Sept. 14, 2016, 2:30 p.m.
    ...11.2 (2016): e0149483. Rogers, Jameson K., and George M. Church. "Genetically encoded sensors enable ...Sciences 108.38 (2011): 15870-15875. See Kendall Morgan’s blog post on the PUF Assembly Kit for some insights...to deposit a kit, please contact us at depost@addgene.org. ...

  9. An Integrin Antibody Toolkit from IPI

    Type
    Blog Post
    Published
    March 28, 2024, 1:15 p.m.
    ...extended-open integrins enable tissue morphogenesis, organ development, and the shaping of the extracellular...subunits (p. 2024.01.26.577394). bioRxiv. https://doi.org/10.1101/2024.01.26.577394 Jo, M. H., Li, J., Jaumouillé...mechanics. Nature Communications, 13(1), 7471. https://doi.org/10.1038/s41467-022-35173-w More resources from ...

  10. Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping

    Type
    Blog Post
    Published
    Dec. 2, 2019, 2 p.m.
    ... to living cells thus APEX is not suitable for organoid or in vivo studies (Che and Khavari, 2017). Find...many fusion proteins this would lead to protein/organelle aggregation. The Ting lab hypothesized that the...Efficient proximity labeling in living cells and organisms with TurboID." Nature biotechnology 36.9 (2018...

  11. Sequencing Options for CRISPR Genotyping

    Type
    Blog Post
    Published
    Oct. 4, 2016, 2:30 p.m.
    ...impact of CRISPR editing in the model cell line or organism. Several options exist for unbiased off-target...equitable society. Read more at https://magazine.scienceforthepeople.org/.     References 1. Fu, Yanfang, et...Models with CRISPR/Cas9 Additional Resources on Addgene.org View Our CRISPR Guide Pages Find CRISPR Plasmids...

  12. 9 tips for a successful postdoctoral experience

    Type
    Blog Post
    Published
    Oct. 9, 2018, 12:21 p.m.
    ...skilled at presenting and answering questions. Organizing a thoughtful engaging talk takes significant ...successful writers about their strategies for organizing and self-critiquing their own writings. 8. Learn... our transferable skills guide Resources on Addgene.org Learn more about Careers at Addgene Watch out...

  13. With an Eye Towards the Future, We Look Back at the March for Science

    Type
    Blog Post
    Published
    Oct. 24, 2017, 1:54 p.m.
    ...the groundbreaking research happens, sometimes forgetting how to engage with friends, family, and the public...over in silence his very large one” (http://oll.libertyfund.org/titles/galilei-dialogues-concerning-two-new-sciences...I was also incredibly impressed with how well organized the march was, as well as the friendly atmosphere...

  14. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    Published
    April 17, 2018, 1:37 p.m.
    ...embryoid bodies which are compact balls of loosely organized tissues that express markers of all three germ...Central PMCID: 2956203 5. Robinton, Daisy A., and George Q. Daley. "The promise of induced pluripotent stem...the Stem Cell Field Additional Resources on Addgene.org Visit our Stem Cell Guide Page Find reprogramming...

  15. Addgene is Stopping Use of X (formerly Twitter)

    Type
    Blog Post
    Published
    Jan. 11, 2024, 2:15 p.m.
    ...decision allows us to remain aligned to our organizational values.  To preserve the knowledge, conversations...can reach out to us directly via email at help@addgene.org, and we invite you to follow us across our social...

  16. Trends in CRISPR and SynBio Technologies

    Type
    Blog Post
    Published
    Feb. 4, 2015, 3:58 p.m.
    ...forums such as http://www.genome-engineering.org and http://www.egenome.org." At the Keystone Meeting Eric...

  17. New FLIS Plasmid (April Fools 2022)

    Type
    Blog Post
    Published
    April 1, 2022, 1:15 p.m.
    ...size have allowed scientists to use it as a model organism to interrogate many scientific questions. But ...containing escaped flies. Nature 4:1-22. https://doi.org/4.01.2022/hafd...

  19. Technical Design of a Western Blot

    Type
    Blog Post
    Published
    Aug. 22, 2024, 1:15 p.m.
    ...gel-to-gel.  Ladder Whatever gel type you select, don’t forget to save a lane for your standards (ladder). You...AntibodyAntibodies 101: Secondary Antibodies Resources on Addgene.org Addgene's Western Blot Protocol Addgene's Western...Neuroscience Methods, 172(2), 250–254. https://doi.org/10.1016/j.jneumeth.2008.05.00...
Showing: 341 - 360 of 809 results