...double-strand break (DSB). DSBs can be caused by intracellular factors such as nucleases and reactive oxygen...for DNA repair synthesis. The ssDNA strand then displaces one strand of the homologous DNA donor and can...formation of a DNA structure referred to as the displacement loop (D loop). The recombination intermediates...synthesized portion of the invasive strand is displaced from the template and returned to the processed...

...precision editing of the dystrophin gene would take place, with minimal risk of off-target editing. In order...sites. Each paper characterizes additional, unique facets of CRISPR DMD therapy. Long et al. show that AAV-CRISPR...the desired phenotype. Precision genome editing faces the challenges above and more. One chief challenge...approval. Even with the challenges precision editing faces, it’s encouraging to see such progress in the more...

...finding yourself having issues, you’re in the right place! In this post, we’ll talk about western blot optimization... visible to the naked eye.   If you see a smiley face in your gel, or if you see smeared bands, your voltage...voltage is likely too high. If you’re seeing a smiley face at an appropriate voltage (usually 10–15 V/cm of...bleed!), cut off the upper left-hand corner, or place a double ladder on the lefthand side. Whatever you...dry the membrane, wet it with 20% methanol, and place over a light box to visualize proteins after transfer...

... site and adjacent PAM. Your knock-in design may naturally remove the gRNA sequence or place it far from...100 bp homology on each side. For optimal repair, place the loxP sites precisely at the cut site for each...the oviductal lumen, tweezer-type electrodes are placed to mediate electroporation. Ohtsuka et al. compared...

...members of your field, and present your research. Face to face meetings can make favorable impressions on potential...Medical Institute. Today's postdoctoral fellows (PFs) face a number of challenges ranging from long periods...manuscripts. 4. Balance focus and exploration Researchers face a fundamental conflict. Science is about exploration...

...reaching the detector. The pinhole is specifically placed to only allow light from the focal plane to reach...coverslip at an angle such that when it reaches the interface between the glass coverslip and a sample in aqueous...within a few hundred nanometers of the glass/water interface. Super resolution microscopy Super Resolution ...sample thin (< 15 μm) or thick? These questions will place each sample into one of four categories that are...

...plasmids and your viral packaging service in the same place, making it easier to go from selection to viral ...to order at your convenience. After an order is placed, we’ll facilitate the necessary MTA, then start...your plasmid of interest in your desired serotype, place your order when ready, and we’ll take care of the...

...Can you imagine how much space a million plasmids would fill? Two weeks ago, Addgene edged passed our... hard to picture one million plasmids. How much space would one million bacterial stabs take up? If 81...upcoming months, we will be moving into a bigger space so that we can make an even bigger impact - we are...

...depends on a surface glycoprotein – the only viral protein present on the virion surface – making it critical... system in a novel way, by shielding host cell surface proteins. Researchers at the University of Virginia...

...factors – to specific sequences of DNA has become commonplace. This ability has opened up a world of possibilities...on a version that she hopes will change the intracellular localization of RNA. “You can think of changing...tips: First, she recommends using standard BsaI in place of the high fidelity version (despite what the original...