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Showing: 541 - 570 of 744 results

  1. 3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells

    Type
    Blog Post
    Published
    Sept. 5, 2017, 1:58 p.m.
    ...them back to wild type), differentiate them into somatic cells affected by the disease and study what’s ...guide RNA binding site works well. However, since some guide RNAs may tolerate a mismatch in the binding...

  2. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

    Type
    Blog Post
    Published
    Oct. 11, 2016, 2:30 p.m.
    ...plant breeding approaches, these techniques are cumbersome, often requiring several rounds of selection ...functionality of the CRISPR system in most organisms, some plant-specific changes to CRISPR components are ...

  3. An Integrin Antibody Toolkit from IPI

    Type
    Blog Post
    Published
    March 28, 2024, 1:15 p.m.
    ...bind discrete subunits or particular subunit pairs. Some of the antibodies in the collection are able to ...hybridoma antibodies developed in the Springer lab. Some are rabbit immunoglobulin G1 (IgG1) chimeric antibodies...

  4. Antibodies 101: Single Chain Fragment Variables (scFvs)

    Type
    Blog Post
    Published
    June 3, 2021, 1:15 p.m.
    ...configurations can generate functional scFvs; however, some individual scFvs performing better in one configuration... of screening. Created with Biorender.com.   Some phage display libraries screen antibody genes from...

  5. Technique: Probe Phage Genomes for Host Binding Proteins

    Type
    Blog Post
    Published
    Sept. 29, 2016, 2:30 p.m.
    ...of the vector. In order to reduce the chances of some clones being outcompeted by others in liquid culture...extraction reagent (BPER from Thermo works well) with some added lysozyme, DNAse I, and protease inhibitor ...

  6. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    Published
    Sept. 24, 2020, 1:15 p.m.
    ... was really due to the intended edit rather than some passenger variant that came along with the single...likely to be active, not only for SpCas9 but also for some other Cas enzymes. It seems to be the case that ...

  8. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    Published
    Jan. 6, 2015, 5:21 p.m.
    ...work than using established methods and minimizes some of the technical issues associated with high levels...Classic genetic engineering methods enabling chromosomal integration of sequences in bacteria are time-consuming...

  9. Beginner's Guide to Viral Vectors

    Type
    Blog Post
    Published
    June 2, 2017, 2:30 p.m.
    ...'s out there, Addgenie Leila Haery has summed up some of the most important characteristics of retroviruses...

  12. 2022 Depositor Week

    Type
    Blog Post
    Published
    Dec. 12, 2022, 2:15 p.m.
    ...Twitter, so keep an eye out there for a chance to win some cool Addgene swag.  And, of course, to all our depositors...

  14. The Twelve Days of Antibodies

    Type
    Blog Post
    Published
    Jan. 6, 2025, 5:17 p.m.
    ...Every year, we like to add in some holiday cheer to our blog and social media. This year, we're doing...

  15. CRISPR Guide Update

    Type
    Blog Post
    Published
    Oct. 10, 2024, 1:15 p.m.
    ...we have added new sections to share and describe some incredible new CRISPR technologies developed and...

  16. Meet Aavery, Blugene's New Virus Friend!

    Type
    Blog Post
    Published
    Feb. 13, 2020, 2:34 p.m.
    ... asking you to help us make the decision between some meaningful names…. Watch the video to find out the...

  17. Addgene's 20th Anniversary Party

    Type
    Blog Post
    Published
    June 11, 2024, 1:15 p.m.
    ...Addgene's past. Framed photos and slideshows shared some of the moments we captured over the years, including...

  18. The 12 Days of CRISPR

    Type
    Blog Post
    Published
    Jan. 3, 2023, 2:15 p.m.
    ...Every year we celebrate the 12 Days of CRISPR with some of the latest and greatest in CRISPR tools and resources...

  19. The 12 Days of CRISPR: 2021

    Type
    Blog Post
    Published
    Dec. 28, 2021, 2:15 p.m.
    ...Addgene gave to me: MUCICAT to perform multicopy chromosomal integration.  On the tenth day of CRISPR, Addgene...

  24. Addgene at SfN23!

    Type
    Blog Post
    Published
    Nov. 9, 2023, 2:15 p.m.
    ...to brush up on your conference skills, check out some of our helpful conference blog posts before you ...

  26. Top Requested Plasmid of 2017 - pMD2.G

    Type
    Blog Post
    Published
    Jan. 10, 2018, 4:13 p.m.
    ...Plasmid technologies are constantly evolving, but sometimes a technology is so useful it forever enhances ...

  28. Bringing Renewable Energy to Addgene

    Type
    Blog Post
    Published
    Aug. 27, 2019, 1:08 p.m.
    ...question cannot be washed or reused/repurposed in some fashion. Addgenies swapping out printer cartridges...

  29. Call for Guest Bloggers

    Type
    Blog Post
    Published
    March 2, 2016, 3 p.m.
    ...Contribute to the Addgene Blog Possible guest topics Some topics you might consider are: An overview of software...
Showing: 541 - 570 of 744 results