We narrowed to 762 results for: SOM

... for an organization in a position that required some involvement in the grants process and managing programs...resources. 3. Get a mentor, find your people Find someone who is working or has worked as a Grant Professional...

... organization that is committed to understanding some of the basic principles of life and health and works...E. E., Mahoney, J. T., Kojima, Y., Ding, Y., Somasundaram, S., Miller, J. A., Kalmbach, B. E., Radaelli...

...Every few months we highlight some of the new plasmids, antibodies, and viral preps in our repository...number of cell types at different genomic loci — in some cases, over 20-fold higher editing than PEmax. The...

...plasmid can transfer to, replicate in, and persist in. Some plasmids have a narrow host range, while others ...fully understood, but scientists have identified some of the features that confer broad host range. These...

...compared to wild-type SpCas9, which also displays some cleavage with an NGA PAM. This variant may increase...potentially reversible since there is no genomic edit. Some Cas13 enzymes require a single base protospacer ...

...talk is quite simple. It's you, a dry erase board, some markers (chalk is rare these days), and a room of..., if you cannot describe the likely outcome with some specifics, the search committee member cannot either...

...generate seven new prime editors, PE6a–g, that overcome some current prime limitations and enable impressive ...protein interactions or map a local proteome, but some applications have been challenging or inaccessible...

...cultures are inspected for sterility. Transducibility Some viral vectors are tested in vitro and in vivo for...gene expression and/or function. These data are sometimes reported or posted on the material page for the...

...anyone who has worked with individual plasmids knows, some plasmids amplify well and others don’t. The size...s far better to verify via NGS now than to learn some of your plasmids didn’t amplify after your screen...

...variety of related bacteria with similar Cas systems. Some of these Cas systems have enzymes small enough to...could likely do it using Prime editing alongside some creative (and significant!) protein engineering....

...be affected by many experimental factors. To list some complicating factors for quantifying AAV: qPCR... or if the sample needs to be quantified again. Some of the issues with titrating by qPCR are addressed...

...Creative titles and controversial topics can garner some attention, though in reality much of our research...about your research is not hard either. Here are some resources: ImpactStory, a website that helps researchers...

...the independent variable you are testing and not some other unintended factor. Control plasmids are used...different days, etc, but outside constraints may impose some limitations and you should be cognizant of these...

...nucleus, or visualizing DNA instead of proteins. Some stains can diffuse through the cell membrane freely...depending on the location of the targeted markers. Some commercially available fix/perm kits allow for labeling...

...immunostaining of the truncated YFP. In Thy1-Brainbow mice, some neurons expressed as many as 16 copies of the injected...cell, even axons of up to 100 microns in length. Some challenges of the Brainbow system are that it does...

...configurations can generate functional scFvs; however, some individual scFvs performing better in one configuration... of screening. Created with Biorender.com.   Some phage display libraries screen antibody genes from...

...them back to wild type), differentiate them into somatic cells affected by the disease and study what’s ...guide RNA binding site works well. However, since some guide RNAs may tolerate a mismatch in the binding...

...bind discrete subunits or particular subunit pairs. Some of the antibodies in the collection are able to ...hybridoma antibodies developed in the Springer lab. Some are rabbit immunoglobulin G1 (IgG1) chimeric antibodies...

...plant breeding approaches, these techniques are cumbersome, often requiring several rounds of selection ...functionality of the CRISPR system in most organisms, some plant-specific changes to CRISPR components are ...

...serotypes have a primary cellular receptor and sometimes one or multiple coreceptors, which may be present...present on different cell types and sometimes vary between target species or even strains. Pro tip! Getting...

...of the vector. In order to reduce the chances of some clones being outcompeted by others in liquid culture...extraction reagent (BPER from Thermo works well) with some added lysozyme, DNAse I, and protease inhibitor ...

... was really due to the intended edit rather than some passenger variant that came along with the single...likely to be active, not only for SpCas9 but also for some other Cas enzymes. It seems to be the case that ...

...construction of plasmid vectors and/or rely on cumbersome in vitro synthesis of single guide RNAs (sgRNAs...of knock-in experiments depends on many factors. Some of these include: Most importantly, the crRNA...

...work than using established methods and minimizes some of the technical issues associated with high levels...Classic genetic engineering methods enabling chromosomal integration of sequences in bacteria are time-consuming...

...'s out there, Addgenie Leila Haery has summed up some of the most important characteristics of retroviruses...

...We’re so excited to share some good news with you: with contributions from depositors around the globe...

...and even those who might just need a refresher on some best practices for introductory science techniques...

...Twitter, so keep an eye out there for a chance to win some cool Addgene swag.  And, of course, to all our depositors...

...your best ideas because the finalists will receive some newly designed Addgene swag. Have fun! Suggest a...

...Every year, we like to add in some holiday cheer to our blog and social media. This year, we're doing...