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We narrowed to 777 results for: REP

Showing: 361 - 380 of 777 results

  1. Plasmids 101: TOPO Cloning

    Type
    Blog Post
    Published
    Oct. 27, 2016, 2:30 p.m.
    ...rejoining supercoiled DNA ends to facilitate replication. The TOPO technique specifically uses Vaccinia...limit (lower transformation efficiencies have been reported with longer incubation); however, you may need...fairly quick, make sure you stay organized and prepare everything you need for the next step before proceeding... Perform Site Directed Mutagenesis by PCR Use REPLACR Mutatgenesis to Easily Generate Insertion and Deletions...

  2. How Dry Ice Affects Viral Vector Sample pH & How to Avoid It

    Type
    Blog Post
    Published
    June 20, 2017, 2:30 p.m.
    ...some unexpected results. Order Ready to Use Viral Preps from Addgene Measuring the pH of our samples: What...closed dry ice storage container for 48 hours (to replicate how our viruses are shipped), where they would...., 2014). Based on our results and the results reported elsewhere, we recommend that once you receive ... CRISPR User) Tips for Titering Your Lentivirus Preps Additional Resources on Addgene.org Check out ...

  3. Virtual Science Conference Coming Up? Three Addgenies Share Their Experience

    Type
    Blog Post
    Published
    June 30, 2020, 1:15 p.m.
    ...and Addgenie Angela Abitua hosted a virtual Reproducibility for Everyone workshop at TAGC with the three...engaged through a computer screen. For the Reproducibility for Everyone Workshop, Abitua and the other...knowing you're suppose to draw Spongebob? The Reproducibility for Everyone Workshop included this interactive... activity during their session. Image from Reproducibility for Everyone.  While this type of interaction...

  4. Plasmids 101: Gram Negative and Positive Bacteria

    Type
    Blog Post
    Published
    Aug. 2, 2022, 1:15 p.m.
    ...species of bacteria. The stain colored one type, Streptococcus pneumoniae, while the alcohol used in the staining...Klebsiella pneumoniae. After realizing this was a repeatable phenomenon, Gram began using it as a classification... positive bacteria include staphylococci and streptococci. Gram negative bacteria, or diderms, have a ...specific procedural instructions, including timing, preparing the smear, and mixing the stains and solutions...

  5. Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering

    Type
    Blog Post
    Published
    May 3, 2018, 1:48 p.m.
    ... bioinformatic screens that looked for a CRISPR repeat array near a putative effector nuclease. The Cas13d...localization signal (NLS). In a HEK293 mCherry reporter assay, CasRx and AdmCas13d produced 92% and 87%...processed gRNA array (22 nt spacer with 30 nt direct repeat) mediating potent knockdown. Multiplexing from ...Addgene Blog: Learn about CRISPR RNA Editing with REPAIR Get tips for using Cas9 Transcriptional Activators...

  6. Cancer, Inflammation and Immunity - Harnessing the Body’s Defenses to Fight Cancer

    Type
    Blog Post
    Published
    July 6, 2017, 1:59 p.m.
    ...immunotherapy, as well as new approaches to reprogramming tumor-associated inflammation for therapeutic...reflect how cancer/immunology researchers are preparing and carrying out this multi-pronged attack. Inflammation...This immunosuppressive environment in turn may represent a favorable state for tumor progression (Ref 2...potential to become an open-source pipeline and data repository for cancer researchers. Go open-source! Impressions...

  7. Multiplex Genome Editing with CRISPR-Cpf1

    Type
    Blog Post
    Published
    May 9, 2017, 2:12 p.m.
    ... et al used four oligos that consist of direct repeats and crRNA.  Similar to a jigsaw puzzle, the oligos... reverse the orientation of the crRNA’s direct repeats when expressing the Cpf1 multiplex system from ...virus.  Reversing the orientation of the direct repeat protects the (+) stranded lentivirus RNA from Cpf1...This undoes the original reversing of the direct repeats, leading to expression of a crRNA array that can...

  8. Viral Vectors 101: Biosensors

    Type
    Blog Post
    Published
    July 6, 2023, 1 p.m.
    ...thing’ could be a neurotransmitter, such as norepinephrine, or an electrolyte such as calcium. This varied...transducer will generate a numerical value which represents the amount of analyte present in the system at...These sensors have been tested in vivo, in tissue preps, in multiple species, and in cell culture models... several years!). Biosensors tend to be more reproducible when used as stable systems, rather than transiently...

  9. Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems

    Type
    Blog Post
    Published
    May 19, 2020, 1:15 p.m.
    ...sites (thrombin and TEV), and C-terminal tags (StrepII, 3xFLAG, 6xHIS, MBP, Myc). These constructs include...manually transforms, innoculates, and performs DNA minipreps for small batches of samples. As soon as that ...that process is complete, QC scientists prepare Sanger reactions by mixing this isolated plasmid DNA with... each group of plates will have select samples prepared and screened by NGS to spot check the samples,...

  10. Viral Vectors 101: Gamma-Retroviral Packaging Systems

    Type
    Blog Post
    Published
    Aug. 6, 2024, 1:15 p.m.
    ...The transgene sequence is flanked by long terminal repeat (LTR) sequences, which facilitate integration of... be used interchangeably. Are γ-retroviruses replication incompetent? Yes, as with lentiviruses, the genes...Subcomponents: -U3 -R -U5 LTR; Long terminal repeats; U3-R-U5 regions found on either side of a retroviral...activation of viral genomic RNA transcription. R; Repeat region found within both the 5' and 3' LTRs of ...

  11. Sweating the Small Stuff: Details in the Lab

    Type
    Blog Post
    Published
    Feb. 8, 2022, 2:15 p.m.
    ...spend more prep time on an experiment than it is to rerun an experiment, so consider your prep an investment... used to constantly underestimate time needed to prep for an experiment (labeling tubes never takes longer...harder to start earlier, but simply moving all my prep work to the day before, which got rid of the issue...

  12. Antibodies 101: Monoclonal Antibodies

    Type
    Blog Post
    Published
    June 29, 2021, 1:15 p.m.
    ... are first injected with a small peptide that represents a short, unique amino acid sequence found in ...cells and antibody-producing B cells that cannot replicate, meaning that only hybridomas will survive this...monoclonal antibodies, they also lead to more reproducible experimental results. When you are originally...about the Developmental Studies Hybridoma Bank, a repository for hybridomas  Learn about monoclonal antibodies...

  13. Plasmids 101: Blue-white Screening

    Type
    Blog Post
    Published
    June 4, 2015, 1:03 p.m.
    ...accurate, IPTG binds and inactivates the lac operon repressor, thereby allowing lac expression). When expressed...multiple cloning site (MCS) into the α-peptide (represented as an orange wedge in the figure on the left)...-complementation (cell B). As shown on the representative plate to the right, colonies with an insert-containing...defined medium, without which the cells cannot reproduce to form colonies (used to complement auxotrophic...

  14. Will You Be My Mentor? Finding and Asking for Mentoring Support

    Type
    Blog Post
    Published
    Feb. 4, 2014, 3:50 p.m.
    ... get started with mentoring. Read this Catalyst Report “Making Mentoring Work” to learn about what makes...mentoring can work if both parties are diligent about replying and thinking about good questions and answers....Thank them anyway and move on. If they say yes, be prepared with questions and topics to discuss. The mentee...make your mentor want to keep working with you is report back with progress. Tell them how their advice ...

  15. Kiran Musunuru on the Newest TALEN Genome-Editing System

    Type
    Blog Post
    Published
    Dec. 10, 2013, 3:13 p.m.
    ...break in the genome anywhere you would like. Cells repair those breaks in one of two ways: by putting the... a gene. One can also exploit homology-directed repair by flooding the cell with a mutant template and...and fooling the cell into using that template for repair. By using these two scenarios, you can either knock...where particular variants or mutations have been reported in people, we can use this kit to knock in those...

  16. RaPID Detection of RNA-protein Interactions

    Type
    Blog Post
    Published
    Aug. 16, 2018, 12:40 p.m.
    ...little was one minute BASU generated a strong streptavidin signal (~250-fold enrichment) when compared ...Ramanathan et al. also used the Contamination Repository for Affinity Purification (CRAPome), to identify...identify highly biotinylated background proteins reported by other research groups that performed BirA*-based... that QKI is a host protein critical to Zika replication. Human genetic disease. RaPID can also be used...

  17. Choosing the B(right)est Fluorescent Protein: Photostability

    Type
    Blog Post
    Published
    June 8, 2017, 2:30 p.m.
    ...conditions, i.e. with low excitation power. By repeating these measurements for different fluorescent proteins... be noted that the photostability measurements reported in the literature are performed in different ways...information can help guide your understanding of reported data. Photobleaching results in a loss of fluorescence...a set-up has been changed (for instance after replacing a broken lightbulb or exchanging excitation filters...

  18. The Breast Cancer Microenvironment: A Tumor’s Backstage Team

    Type
    Blog Post
    Published
    Jan. 31, 2019, 1:32 p.m.
    ...How do microenvironment enzymes and metabolic reprograming influence tumors development? Being pathological...cells improve tumor’s durability, while others repress it. Unfortunately, as a whole, microenvironments...microenvironments promote tumor durability instead of repressing them, making it even harder to cure cancer. To understand...affect tumor progression, enzymes and metabolism reprogramming aren’t the whole story. It’s essential for a...

  19. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

    Type
    Blog Post
    Published
    March 3, 2020, 2:15 p.m.
    ...highlight a subset of the new plasmids in the repository through our hot plasmids articles. These articles...indicator plasmids at Addgene! Beck et al., Sci Report 2019. PubMed PMID: 31685893. A MoClo toolkit for... standardized parts, it allows for greater reproducibility as scientists can assemble parts from different...sequences for genomic integration by homology directed repair. Using parts from this kit, the McCormick lab generated...

  20. Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening

    Type
    Blog Post
    Published
    April 12, 2016, 2:30 p.m.
    ...AATTGXXXXXXXXGAATAGGTCTTCTCGAAGACCCAAACNNNNNNNNNNNNNNNNNNNC -3’, where NNN and XXX represent the 20-bp gRNA target sequence and its 8-bp barcode...and applications in functional genomics, cell reprogramming, and beyond. For further details, please check..."Genome-scale CRISPR-mediated control of gene repression and activation." Cell 159.3 (2014): 647-661. ... for West-Nile-virus-induced cell death." Cell reports 12.4 (2015): 673-683. PubMed PMID: 26190106. PubMed...
Showing: 361 - 380 of 777 results