We narrowed to 582 results for: RON

...CRISPR-based RNA editing - and further experiments strongly supported their initial findings.  The team originally...the chance of the insert being “dropped” in the wrong place, while increasing the number of potential ...

...school and beyond is to live in your lab, right? Wrong! While it is clearly a good idea to be diligent ...them for work. Addgene’s very own Joanne Kamens, a strong advocate for career planning, has written a great...

...- there's a simple method for that! By Michelle Cronin The benefits of nanobodies over conventional antibodies...optogenetic AAV for expression in forebrain GABA-ergic interneurons. These include mDlx-GFP, hDlx-FLEX-GFP, hDlx-Flex-dTomato...

... Martin et al., 2018). The ACE reporter is a bicistronic construct that consists of a mutated mCherry ... https://doi.org/10.1038/mtna.2016.48 Katti A, Foronda M, Zimmerman J, Diaz B, Zafra MP, Goswami S, Dow...

...development and evolution of histone genes in Strongylocentrotus purpuratus, the spiny purple sea urchin. ...demonstrate unusual parallel adaptation to disparate environments so can be used study the genetic basis of evolution...

...it’s like to be properly onboarded. Most work environments outside of academia provide a formal training... until I was out of the lab and in an office environment. Ultimately, it will likely be these skills that...

...from GenBank database (CUTG), that provides an electronic dataset for codon-usage of 257,468 genes across...scientists with the ability to selectively silence neurons in vivo (Slimko and Lester, 2003).  Addgene has...

...Laboratory Golden Gate Cloning videos made by the Nicola Patron group, one of the developers of the MoClo system...extracting and sequencing the plasmid, or both! We strongly encourage you to sequence the entire insert region...

...bacterium picking up resistance genes from it’s environment (from DNA left behind after another bacterium...survival. Existing virtually ubiquitously in the environment, there is an intense amount of competition between...

... the most rewarding parts of the job is being confronted with challenges that are crucial for the success...in a short amount of time can sometimes become cauldrons on the verge of boiling. On other occasions, clients...

...however, if the microinjection was done as the pronuclei were fusing, you may have several mosaic mice....Daisuke, et al. "Generation of mutant mice by pronuclear injection of circular plasmid expressing Cas9...

...system could be as simple as a piece of paper on the front of the incubator containing the passage number, ...ensure the results are reliable. With this two-pronged approach, the many mistakes of the past can be ...

...onto data longer before publishing. But there are strong incentives to publish such as precedence, recognition...Lunenfeld-Tanenbaum Research Institute at Mount Sinai Hospital, Toronto, Canada where he studies protein-serine/threonine...

...light-sensitive ion channels used to control activities like neuronal signaling — think of a mouse with an LED brain... — but there’s a lot more to optogenetics than neuronal activity. Researchers have designed a variety ...

... Many cell types can be found in the tumor microenvironment (tumor cells, immune cells, blood cells and...recorded events) based on their expected size. The strong signals close to the graph’s origin mainly stem...

... cells affected by the disease and study what’s wrong with the cells. However, our excitement turned into...picking 100 versus 10,000 clones! We would therefore strongly encourage any researcher to incorporate CRISPR...

...compared to one pot saturation mutagenesis. Error-prone PCR: This method amplifies the wild-type template...Chaput, J. C. (2010). “Random Mutagenesis by Error-Prone PCR”. Methods in Molecular Biology In Vitro Mutagenesis...

...beta-barrel structure. Although the beta barrel has no strong affinity for other proteins, it may still block...knock-out of the corresponding protein, that is strong evidence that the fusion behaves well. But this...

...the DNA backbone, making them significantly less prone to indel mutations that plagued CRISPR editing at... K. T., Eton, E., Lapinaite, A., Newby, G. A., Thuronyi, B. W., Wilson, C., Koblan, L. W., Zeng, J., Bauer...

...common promoters are U6, H1, and 7SK. These are strong promoters with no tissue specificity, so in some...biogenesis, mechanisms of actions, and circulation. Frontiers in Endocrinology, 9. https://doi.org/10.3389/fendo...