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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 580 results for: RON

Showing: 361 - 380 of 580 results

  2. New CRISPR Tools: Cas7-11 and PASTE

    Type
    Blog Post
    Published
    Feb. 17, 2022, 2 p.m.
    ...CRISPR-based RNA editing - and further experiments strongly supported their initial findings.  The team originally...the chance of the insert being “dropped” in the wrong place, while increasing the number of potential ...

  3. Transferable Skills Guide: Career Planning Resources

    Type
    Blog Post
    Published
    Nov. 14, 2017, 2 p.m.
    ...school and beyond is to live in your lab, right? Wrong! While it is clearly a good idea to be diligent ...them for work. Addgene’s very own Joanne Kamens, a strong advocate for career planning, has written a great...

  4. Tips for Writing a Good Cover Letter

    Type
    Blog Post
    Published
    May 21, 2024, 1:30 p.m.
    ...reproducible assay. That information can make you a much stronger candidate!  Additional uses The cover letter is...job with a lot of file management that asks for strong organizational skills. You might think, “I’d be...

  5. New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression

    Type
    Blog Post
    Published
    Jan. 24, 2023, 2:15 p.m.
    ...visible under both phase contrast microscopy and electron microscopy (Fig. 1b). Gas vesicle expression ...batch-sorted to isolate a polyclonal cell line with strong gas vesicle expression and robustness without chromatin-opening...

  6. Lab to Office Culture Shock

    Type
    Blog Post
    Published
    Feb. 5, 2019, 1:04 p.m.
    ...it’s like to be properly onboarded. Most work environments outside of academia provide a formal training... until I was out of the lab and in an office environment. Ultimately, it will likely be these skills that...

  7. Antibiotic Resistance: An Old Solution but a New Problem

    Type
    Blog Post
    Published
    Nov. 10, 2016, 3:30 p.m.
    ...bacterium picking up resistance genes from it’s environment (from DNA left behind after another bacterium...survival. Existing virtually ubiquitously in the environment, there is an intense amount of competition between...

  8. Healthcare Consulting: A Door to the Business of Life Sciences

    Type
    Blog Post
    Published
    Oct. 13, 2016, 2:30 p.m.
    ... the most rewarding parts of the job is being confronted with challenges that are crucial for the success...in a short amount of time can sometimes become cauldrons on the verge of boiling. On other occasions, clients...

  9. Tips for CRISPR Gene Editing in Mice

    Type
    Blog Post
    Published
    June 28, 2016, 10:59 a.m.
    ...however, if the microinjection was done as the pronuclei were fusing, you may have several mosaic mice....Daisuke, et al. "Generation of mutant mice by pronuclear injection of circular plasmid expressing Cas9...

  10. The Challenges of Cell Culture

    Type
    Blog Post
    Published
    May 5, 2016, 2:30 p.m.
    ...system could be as simple as a piece of paper on the front of the incubator containing the passage number, ...ensure the results are reliable. With this two-pronged approach, the many mistakes of the past can be ...

  11. Data Freedom: The Expansion of Data Sharing in Research Publications

    Type
    Blog Post
    Published
    Aug. 5, 2014, 6:51 p.m.
    ...onto data longer before publishing. But there are strong incentives to publish such as precedence, recognition...Lunenfeld-Tanenbaum Research Institute at Mount Sinai Hospital, Toronto, Canada where he studies protein-serine/threonine...

  13. The Advances Behind The World's First Personalized CRISPR Treatment

    Type
    Blog Post
    Published
    Sept. 16, 2025, 1:15 p.m.
    ...the DNA backbone, making them significantly less prone to indel mutations that plagued CRISPR editing at... K. T., Eton, E., Lapinaite, A., Newby, G. A., Thuronyi, B. W., Wilson, C., Koblan, L. W., Zeng, J., Bauer...

  14. Plasmids 101: Expressing shRNAs for RNA Interference

    Type
    Blog Post
    Published
    June 17, 2025, 1:15 p.m.
    ...common promoters are U6, H1, and 7SK. These are strong promoters with no tissue specificity, so in some...biogenesis, mechanisms of actions, and circulation. Frontiers in Endocrinology, 9. https://doi.org/10.3389/fendo...

  15. Hot Biosensors 2022: Year-End Roundup

    Type
    Blog Post
    Published
    Dec. 6, 2022, 2:15 p.m.
    ...MTRIA sensors will be useful for investigating neuronal signaling and for engineering improved sensors...Small Near-Infrared Fluorescent Calcium Indicator. Front Cell Dev Biol. 2022 Jun 29;10:880107. doi: 10.3389...

  16. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    Published
    Sept. 28, 2023, 1:15 p.m.
    ...light-sensitive ion channels used to control activities like neuronal signaling — think of a mouse with an LED brain... — but there’s a lot more to optogenetics than neuronal activity. Researchers have designed a variety ...

  17. 3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells

    Type
    Blog Post
    Published
    Sept. 5, 2017, 1:58 p.m.
    ... cells affected by the disease and study what’s wrong with the cells. However, our excitement turned into...picking 100 versus 10,000 clones! We would therefore strongly encourage any researcher to incorporate CRISPR...

  18. Antibodies 101: Introduction to Gating in Flow Cytometry

    Type
    Blog Post
    Published
    April 4, 2024, 1:15 p.m.
    ... Many cell types can be found in the tumor microenvironment (tumor cells, immune cells, blood cells and...recorded events) based on their expected size. The strong signals close to the graph’s origin mainly stem...

  19. Deep Mutational Scanning with One Pot Saturation Mutagenesis

    Type
    Blog Post
    Published
    Feb. 22, 2017, 3:30 p.m.
    ...compared to one pot saturation mutagenesis. Error-prone PCR: This method amplifies the wild-type template...Chaput, J. C. (2010). “Random Mutagenesis by Error-Prone PCR”. Methods in Molecular Biology In Vitro Mutagenesis...

  20. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

    Type
    Blog Post
    Published
    Oct. 11, 2016, 2:30 p.m.
    ...Yiping Qi lab and the MoClo Plant Parts Kit from the Patron Lab.  As with all plasmids in the repository, we...Genome-Editing Tool: Application in Improvement of Crops. Front Plant Sci 7: . https://doi.org/10.3389/fpls.2016.00506...
Showing: 361 - 380 of 580 results