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We narrowed to 749 results for: SOM

Showing: 361 - 380 of 749 results

  1. CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!

    Type
    Blog Post
    Published
    Oct. 6, 2020, 1:15 p.m.
    ...methods vary in their transcriptional activators: some methods rely on fusion proteins while others re-... exhibits modest levels of gene activation, with some genes experiencing around 2-fold activation levels...method has shown higher activation levels than SAM in some loci across Drosophila cell lines (Sajwan et al....

  2. Meet an Addgenie: Insights into Grad School, Career Paths, and More

    Type
    Blog Post
    Published
    June 25, 2020, 1:15 p.m.
    ...patterns throughout the project where it solidified some concepts that are heard often during undergrad. ...huge interest in data science,” which she’s taking some classes on and hoping to pursue. The choice to not... does. Her parents told her that if she found something she loves to do, she would never work a day in...

  3. CRISPR in the Clinic

    Type
    Blog Post
    Published
    Nov. 2, 2023, 1:15 p.m.
    ...forward to where it is today. Below we will review some of the CRISPR-based editing therapies that are currently...CRISPR CAR-T treatments for leukemia, lymphoma, and some solid tumors are currently in early-phase clinical...CRISPR-HIV therapy currently in clinical trials is somewhat similar (Hussein et al). It employs Cas9 to cut...

  4. A Look at Addgene's QC Process

    Type
    Blog Post
    Published
    May 6, 2025, 1:15 p.m.
    ...the plasmid to stop functioning properly or be something completely different than what was originally ... other critical functional components. Fun fact! Some older plasmids in our collection do not have a full...based on SnapGene’s “Common Feature” library — with some additional features added by the QC team — and allows...

  5. Adeno Associated Virus (AAV) for Cell and Gene Therapy

    Type
    Blog Post
    Published
    Nov. 7, 2017, 1:59 p.m.
    ...to quickly identify underlying genetic causes of some human conditions, opening up new avenues for therapeutics...that are responsible for integration into host chromosomes, further minimizing potential activation of innate...example, prevalence of neutralizing antibodies against some AAV types, AAV1, 2, 3 and 5 are thought to be as...

  6. Plasmids 101: Terminators and PolyA signals

    Type
    Blog Post
    Published
    March 31, 2016, 2:30 p.m.
    ... initiating the desired cleavage event, although some engineered terminators come close (>95%). For most...presence of the poly(A) tail targets the RNA to the degradosome, which contains enzymes that cut RNA not protected...types of RNA: Polymerase I is responsible for ribosomal RNA, Polymerase II is responsible for mRNA and...

  7. Experimenting in an Open Source lab: from CRISPR to Cats

    Type
    Blog Post
    Published
    July 23, 2015, 2:30 p.m.
    ...and sequencing is being organized and funded by someone else (KittyBiome has been incorporated as a non-profit...bioinformatics pipeline. It’s possible that we’ll find something interesting and contribute it back to the kittybiome...And if not, I suspect you can find a makerspace somewhere near you. The place to start looking for your ...

  8. The Scientific Conference Poster Session: Tips for Success

    Type
    Blog Post
    Published
    Aug. 29, 2019, 12:50 p.m.
    ...yourself: I always find it odd when I walk up to someone’s poster, and then he/she dives right into the background... background without an introduction. If someone approaches your poster, introduce yourself and ask about...you’ve already set yourself up for success. But sometimes, attendees can get sucked in to thirty-minute-...

  9. Chromoproteins: Colorful Proteins For Molecular Biology Experiments

    Type
    Blog Post
    Published
    Feb. 4, 2020, 2:15 p.m.
    ...fitness cost varied greatly between chromoproteins. In some cases, cells containing high-copy plasmid-borne ...though expressing the chromoproteins from the chromosome could get around this problem. There is no single...chromoproteins are and how they can be used, why not add some color into your research? If you have a favorite...

  10. Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles

    Type
    Blog Post
    Published
    June 22, 2017, 2:30 p.m.
    ...may be transported through the vesicles to the lysosome where they are finally degraded (2, 3). Once you've...software tools can be used for colocalization analysis. Some examples include the ImageJ plugins JACoP and Coloc...limitations and well-executed experiments require some consideration of FP properties. It is important ...

  11. Antibodies 101: Conventional vs Spectral Flow Cytometry

    Type
    Blog Post
    Published
    Aug. 3, 2023, 1:15 p.m.
    ...be distinguished using conventional flow can (sometimes) be distinguished using spectral flow since the...signal, even for rare cellular markers, beads can sometimes distort the fluorescent signature upon antibody...Although there are a few aspects of spectral flow that some users may find more difficult compared to conventional...

  12. Hot Plasmids: Winter 2025

    Type
    Blog Post
    Published
    March 11, 2025, 1:15 p.m.
    ...Every few months, we highlight some of the new plasmids, antibodies, and viral preps in the repository...function of the distance between measured pixel and the soma. The initiation site identified in panel (C) is ...-independent versions that are trafficked to the soma via the Kv2.1 targeting motif as well as a pan-membrane...

  13. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

    Type
    Blog Post
    Published
    June 25, 2024, 1:15 p.m.
    ...editing performance, we suggest that users select some of the best designs (e.g., top 5) and test them ...strengths of PRIDICT/PRIDICT2.0 while accounting for some of the inherent inaccuracies of computational prediction...Koeppel et al., 2023), their predictive power is sometimes limited across different targets due to variations...

  14. Pooled Library Amplifications

    Type
    Blog Post
    Published
    May 30, 2024, 1:30 p.m.
    ...achieve. Most pooled library protocols aim for somewhere between 30x and 200x coverage, meaning that you...Rinehart Lab Iterative Synthetically Phosphorylated Isomers (iSPI) Libraries ~11,000–110,139 144-188 bp...closer to your own pooled library. Be aware that some older libraries may not have amplification protocols...

  15. Addgene's Cancer Collection Pages Connect the Research Community

    Type
    Blog Post
    Published
    Sept. 6, 2016, 2:30 p.m.
    ... cellular proliferation. We have appreciated for some time the causes of this uncontrolled proliferation... in place to protect us from rogue cells when something does go awry. The process of programmed cell death...discovering and designing molecules that are showing some promise in their ability to inhibit Ras. Stephen...

  16. Google Forums Round Up: First Impressions of NgAgo

    Type
    Blog Post
    Published
    Aug. 4, 2016, 2:30 p.m.
    ...with the new method and 65% wanted to wait until someone else optimizes an NgAgo protocol for their favorite...research community if you've had success with it. Some great places to get your results out there include...advancing rapidly and it wouldn’t be a surprise if someone came up with a mutant NgAgo (or a novel endonuclease...

  18. PITChing MMEJ as an Alternative Route for Gene Editing

    Type
    Blog Post
    Published
    Feb. 23, 2016, 3:30 p.m.
    ...termed PITCh (Precise Integration into Target Chromosomes). Using their PITCh plasmids, GFP knock-in cell...microhomologies.MMEJ has certain advantages over HDR. Some species don’t have good HDR systems, and NHEJ will...amplification. Given the inefficiency of HDR for knock-ins, some labs have used NHEJ for whole plasmid integration...

  19. CRISPR 101: Mammalian Expression Systems and Delivery Methods

    Type
    Blog Post
    Published
    Sept. 24, 2015, 2:30 p.m.
    ...! In this blog post we’ll provide an overview of some CRISPR mammalian expression systems, the typical...includes several different methods, so we've summarized some key features of common  delivery methods below: ... in terms of CRISPR use, you will want to invest some time in optimizing and testing the efficiency of...

  20. Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

    Type
    Blog Post
    Published
    Dec. 12, 2013, 3:35 p.m.
    ...computer-programming languages? That's trickier. Sometimes it's the developers. But, it turns out over time...engineering qualities, what these parts allow is for someone to forward engineer a system of 10 genes. You can...can now forward engineer a 10-gene system to do something. We know from our testing of these parts that ...
Showing: 361 - 380 of 749 results