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...many times, until you achieve whatever goal you’re aiming for. When you complete each cycle, your best-performing...building things, lots of math, and maybe heavy machinery. But not all engineering is at a large scale —...improved specificity. There are different ways to achieve this goal, including error-prone PCR, degenerate...

...blog post will discuss differences between these approaches, and provide updates on how best to design gRNAs...should not be undertaken lightly! Indeed, truly achieving the gold standard requires not one but two rounds... using knockout or transcriptional modulation approaches. Avoiding off-target effects The off-target ...Guell M, Byrne S, Yang JL, De Los Angeles A, Mali P, Aach J, Kim-Kiselak C, Briggs AW, Rios X, Huang P-Y, ...

...to arrive at this hierarchy, the main aspects of each class and type are easy to understand. Classes are...involved in plasmid competition or can hijack machinery from other CRISPR systems to carry out various...further broken down into type II, type V, and type VI, each relying on a unique Cas effector — Cas9, Cas12, ...basic diversity and the surface-level function of each type could lead you to new directions in your research...

...discuss. If you can think it, MoClo might help you achieve it! MoClo applications and specialized tools Synthetic...applications in bacteria or cell-free systems. The approach is particularly useful for producing drug precursors...a broad range of parts, including copy number machinery, chromosomal integration modules, and many more...can dream up an experiment, MoClo can help you achieve it! More recently, a CRISPR MoClo kit termed Fragmid...

...eBook is designed to be a helpful desktop reference. Each chapter brings together all of our 101 blog posts...collection useful. And, as always, don’t hesitate to reach out to [email protected] with any thoughts, questions... Rachel Leeson...

...Performing In Vivo CRISPR Screens Using the FITS Approach Caitlin Faulds, who introduced the very first...Applications and Kits We are very grateful for each and every contributor to the blog. Thank you! More...  Rachel Leeson...

...generally publish 5-10 Hot Plasmid articles with each of our newsletters. You can find all of these articles...you haven’t got time to sit down and read through each one, plug in some headphones to learn more about...through this podcast segment. Below, we break down each of the Hot Plasmids segments into its own (highly...

...finally December, and here at Addgene HQ in Massachusetts that means it’s time for near-freezing temperatures...This edition of the contest is our traditional approach. You might decorate your lab in pipette tip snowflakes...home editions. We will select up to 3 winners for each edition and the winners will be revealed on Twitter...

...tasked the students to separate out colonies from each individual strain using the streak plate technique...University of Science and Technology, has been teaching the microbiology lab every year for over 20 years...strains are easily amenable to other experiments to teach other aspects of molecular biology. “What I'd like...

... detect a specific molecule. By combining this approach with receptors for problematic ligands, crops ...Gehan’s team also assessed hyperspectral imaging approaches for detecting plant pigmentation from an aerial...of a field of crops taken from above. A trained machine learning algorithm was able to detect anthocyanin...

...these plasmids have a heavy focus on teaching. He hopes to teach his molecular biology students about ...Working on Rare Diseases Read About Some of Our Outreach Efforts in China Additional Resources on Addgene.org...

...Making the Right Connection by Joachim Goedhart Guest blogger Joachim Goedhart shares some strategies...molecule, are often named after vegetables; Broccoli, Spinach, and Corn, for example. Learn more about the aptamer...

... is my pleasure to announce that we once again reached new heights on the Addgene blog: we surpassed 60,000...Proteins," was contributed by guest blogger Jessica Sacher from the University of Alberta and describes a ...currently on my way back from a very successful Outreach Trip in San Francisco and would like to give my...

... huge, there are times when a more incremental approach is prudent. What's in the CRISPR kinome library...available from Addgene as pools provided in two sets, each with 4 gRNAs per kinase, so screens can be performed...gRNA, for use with cells that already have Cas9). Each library consists of approximately 3,000 gRNAs, as...

...need for a faster and higher-throughput in vitro approach. Using data from The Cancer Genome Atlas (TCGA...et al., 2016), creating a collection of plasmids each carrying a single gene with a defined mutation or...the mutations to functional groups, based on how each one impacted cell viability (see figure). Most of...

...sets, each of which includes plasmids with different varieties of tags, both N- and C-terminal. Each set...Central PMCID: PMC4227841. 2. Young, Carissa L., Zachary T. Britton, and Anne S. Robinson. "Recombinant ...

...genomic deletions We were inspired to produce intrachromosomal deletions based on the experiments of Kim ...to explore the paired double-strand break (DSB) approach at a variety of loci. We were pleasantly surprised... RT-qPCR, or immunoblotting to detect. Another approach is to target two exonic sites within a single ...

...works better for you) and place them on an easy to reach shelf. Make sure all notebooks are visibly labeled...code your Post-its and write brief descriptions on each. Place the notes sticking up out of the top of the...from webpage clippings, photographs, and file attachments. Organize your notes into notebooks, create checklists...

...wonderful opportunity to once again reflect on our achievements in the last year and share the exciting things...our website, which receives 3.8 million visitors each year.  This year, we are excited to launch our Antibody...companies, while making use of our nonprofit status to achieve mission-driven goals of accelerating research and...

...Scientists on Twitter. Each dot represents a scientist. Only scientists who follow each other, or are friends...this cluster analysis. The closer two dots are to each other, the more friends they have in common. Only...