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We narrowed to 816 results for: ache

Showing: 361 - 380 of 816 results

  1. A new platform for fast functional characterization of potentially cancerous mutations

    Type
    Blog Post
    Published
    May 7, 2019, 12:39 p.m.
    ...need for a faster and higher-throughput in vitro approach. Using data from The Cancer Genome Atlas (TCGA...et al., 2016), creating a collection of plasmids each carrying a single gene with a defined mutation or...the mutations to functional groups, based on how each one impacted cell viability (see figure). Most of...

  2. Easing the Protein Purification Process with pCri

    Type
    Blog Post
    Published
    June 19, 2015, 3:08 p.m.
    ...sets, each of which includes plasmids with different varieties of tags, both N- and C-terminal. Each set...Central PMCID: PMC4227841. 2. Young, Carissa L., Zachary T. Britton, and Anne S. Robinson. "Recombinant ...

  3. CRISPR Protocol for Genomic Deletions in Mammalian Cell Lines [Video]

    Type
    Blog Post
    Published
    Feb. 18, 2015, 3:09 p.m.
    ...genomic deletions We were inspired to produce intrachromosomal deletions based on the experiments of Kim ...to explore the paired double-strand break (DSB) approach at a variety of loci. We were pleasantly surprised... RT-qPCR, or immunoblotting to detect. Another approach is to target two exonic sites within a single ...

  4. Three Tips to Organize Your Lab Notebooks in the New Year

    Type
    Blog Post
    Published
    Jan. 2, 2014, 3:44 p.m.
    ...works better for you) and place them on an easy to reach shelf. Make sure all notebooks are visibly labeled...code your Post-its and write brief descriptions on each. Place the notes sticking up out of the top of the...from webpage clippings, photographs, and file attachments. Organize your notes into notebooks, create checklists...

  5. Who Gives a Tweet? 9 Facts About Scientists on Twitter

    Type
    Blog Post
    Published
    June 26, 2017, 1:31 p.m.
    ...Scientists on Twitter. Each dot represents a scientist. Only scientists who follow each other, or are friends...this cluster analysis. The closer two dots are to each other, the more friends they have in common. Only...

  6. 2023 and 2024: Reflections and Looking Ahead

    Type
    Blog Post
    Published
    Jan. 23, 2024, 2:15 p.m.
    ...wonderful opportunity to once again reflect on our achievements in the last year and share the exciting things...our website, which receives 3.8 million visitors each year.  This year, we are excited to launch our Antibody...companies, while making use of our nonprofit status to achieve mission-driven goals of accelerating research and...

  7. pSiM24: Simplifying Plant Genetic Engineering

    Type
    Blog Post
    Published
    Sept. 29, 2015, 2:30 p.m.
    ... vectors contain two origins of replication, one each for E. coli and Agrobacterium, along with a selectable...size, they shortened the T-DNA borders to 25 bases each. By switching the E. coli origin of replication ...and codon-optimizing the Agrobacterium ORI, they achieved much higher DNA yield and transformation efficiency...

  8. Cre-ating New Methods for Site-specific Recombination in Drosophila

    Type
    Blog Post
    Published
    May 12, 2015, 1:32 p.m.
    ...representing the specific recombination site for each enzyme, were generated to test recombination efficiency...efficiency. When expressed in neuronal cells, each line showed broad recombinase-dependent RFP expression...determine the cross-reactivity of these recombinases, each recombinase and reporter construct pair were tested...

  9. In Living Color: The Skinny on In Vivo Imaging Tools

    Type
    Blog Post
    Published
    March 27, 2014, 5:14 p.m.
    ...-infrared range, and that’s exactly what iRFPs achieved. Those proteins allowed a signal-to-background...conventional FPs. In addition, PA FPs can improve the achievable signal-to-background ratio, thus, allowing higher...contrast agents for two-color imaging in animals. The approach relies on ultrasound waves as opposed to light...

  11. Antibodies 101: Affinity Tags

    Type
    Blog Post
    Published
    Sept. 19, 2023, 1 p.m.
    ... of affinity tags, the proteins they work best attached to, and the species they are compatible with are...nature of the size, structure, and other qualities of each tag class, they are generally best suited for their...101: Protein tags Antibodies 101: Epitope tags To Each HIS Own ...

  12. An Addgene Co-op: The Intersection of Fun, Friendship, and Work

    Type
    Blog Post
    Published
    June 29, 2023, 7 p.m.
    ...meetings, when Melina, our CSO and co-founder, approached me and asked about my current projects and personal...seeing early results that suggest our content is reaching more people who need it!  After a few months, ...followed with great discussion and questions. I achieved my goal of helping everyone understand the importance...

  13. Plasmids 101: Gene, enhancer, and promoter trapping

    Type
    Blog Post
    Published
    May 2, 2023, 1 p.m.
    ...proximity to an endogenous gene. Each trap uses a slightly different approach (described in detail below),...something species-specific) or a selectable marker. Each insertion line can then be isolated and screened...

  14. Sleeping Beauty Awakens for Genome Engineering

    Type
    Blog Post
    Published
    June 30, 2015, 2 p.m.
    ...protein reporters (GFP, BFP, RFP, no FP). The vectors each contain two SfiI restriction sites with different...RPL41 gene, to drive the selection/reporter parts of each construct. This new tool kit allows the rapid generation...Epub 2009 May 3. PubMed. 3. Singh H, et al. A new approach to gene therapy using Sleeping Beauty to genetically...

  15. Choosing Your Perfect Empty Backbone

    Type
    Blog Post
    Published
    Aug. 19, 2014, 3:39 p.m.
    ...been incorporated into the host organism, usually achieved with the proper selection marker or antibiotic...shuttle vectors. You may prefer the more traditional approach of an autonomously replicating vector that carries....g. pMT-puro). Worm vectors There are several approaches to studying YGOI's function in worms. Some of...

  16. 10 Ways to Share Your Science!

    Type
    Blog Post
    Published
    May 13, 2014, 8:34 p.m.
    ...others in the field where to find it. A good way to reach others in the science blogosphere is to read and... great place to find opportunities for science outreach. Here in the North Carolina Research Triangle ...He got a little money for his trouble, too. 7)   Reach out to science writers in your area or at your institution...

  17. The Stingy Scientist: How the Baby Gel Box Was Born

    Type
    Blog Post
    Published
    April 15, 2014, 3:11 p.m.
    ...recycle our discards according to the Cambridge, Massachusetts guidelines and wear an extra sweater so we don...protocol for making your own Taq polymerase by Outreach Scientist, Andy Baltus. Addgene founder, Melina...illustration above) and bribed the guys in the hospital machine shop with coffee and donuts to cut the pieces of...

  18. Viral Vectors 101: Parts of the AAV Transfer Plasmid

    Type
    Blog Post
    Published
    Aug. 11, 2020, 1:15 p.m.
    ...focus on the AAV transfer plasmid and take a look at each of its parts. Figure 1: Parts of an AAV transfer...AAV transfer plasmid. ITR sequences are 145 bases each and AAV plasmids have two ITR sequences. The DNA...Woodard K, Cepko C, Guerin KI, Rego MA, Ersing I, Bachle SM, Kamens J, Fan M (2019) Adeno-Associated Virus...

  19. Viral Genome Sequencing - A One Stop Shop for AAV Identity and Serotype Confirmation

    Type
    Blog Post
    Published
    April 23, 2020, 1:15 p.m.
    ...TA-based ligation and tagmentation preparation approaches described previously require a preliminary double... sequencing workflow. Using this simplified approach, we sequenced over 100 AAV vector preparations...believe that this simplified sequencing and analysis approach can be easily incorporated into standard QC regimens...

  20. In Vivo Biotinylation of Bacterial Fusion Proteins

    Type
    Blog Post
    Published
    Jan. 25, 2018, 2:09 p.m.
    ...of an experimental protein-ligand interaction approaches that of GST-glutathione. Biotin-based immobilization...located near the ligand binding site. A selective approach entails the use of the enzyme biotin ligase, which... bacteria refuse to grow. Eventual success was achieved by inserting BirA into a pMal plasmid (NEB) thus...
Showing: 361 - 380 of 816 results