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We narrowed to 825 results for: org

Showing: 361 - 380 of 825 results
  1. Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

    Type
    Blog Post
    Published
    Oct. 27, 2015, 3 p.m.
    ...distinct subcellular environments, including the organelles that compose the secretory pathway, endocytic...formation, and proteolytic cleavage. FPs localized to organelles are equally susceptible to these non-native modifications... Lee Snapp. "Fluorescent proteins in cellular organelles: serious pitfalls and some solutions." DNA and...
  2. Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping

    Type
    Blog Post
    Published
    Dec. 2, 2019, 2 p.m.
    ... to living cells thus APEX is not suitable for organoid or in vivo studies (Che and Khavari, 2017). Find...many fusion proteins this would lead to protein/organelle aggregation. The Ting lab hypothesized that the...Efficient proximity labeling in living cells and organisms with TurboID." Nature biotechnology 36.9 (2018...
  3. Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

    Type
    Blog Post
    Published
    April 9, 2019, 1:13 p.m.
    ...the observation of proteins in living cells and organisms. Both components of the chimera are encoded by...). However, these assays are qualitative. The Organized Smooth Endoplasmic Reticulum (OSER) assay (Costantini...Read our plasmids 101 blog series Resources on Addgene.org Browse the fluorescent protein collection Find...
  4. An Integrin Antibody Toolkit from IPI

    Type
    Blog Post
    Published
    March 28, 2024, 1:15 p.m.
    ...extended-open integrins enable tissue morphogenesis, organ development, and the shaping of the extracellular...subunits (p. 2024.01.26.577394). bioRxiv. https://doi.org/10.1101/2024.01.26.577394 Jo, M. H., Li, J., Jaumouillé...mechanics. Nature Communications, 13(1), 7471. https://doi.org/10.1038/s41467-022-35173-w More resources from ...
  5. Multiple Plasmids at a Low Price: Kits from Addgene

    Type
    Blog Post
    Published
    Sept. 14, 2016, 2:30 p.m.
    ...11.2 (2016): e0149483. Rogers, Jameson K., and George M. Church. "Genetically encoded sensors enable ...Sciences 108.38 (2011): 15870-15875. See Kendall Morgan’s blog post on the PUF Assembly Kit for some insights...to deposit a kit, please contact us at depost@addgene.org. ...
  6. 9 tips for a successful postdoctoral experience

    Type
    Blog Post
    Published
    Oct. 9, 2018, 12:21 p.m.
    ...skilled at presenting and answering questions. Organizing a thoughtful engaging talk takes significant ...successful writers about their strategies for organizing and self-critiquing their own writings. 8. Learn... our transferable skills guide Resources on Addgene.org Learn more about Careers at Addgene Watch out...
  7. Sequencing Options for CRISPR Genotyping

    Type
    Blog Post
    Published
    Oct. 4, 2016, 2:30 p.m.
    ...impact of CRISPR editing in the model cell line or organism. Several options exist for unbiased off-target...equitable society. Read more at https://magazine.scienceforthepeople.org/.     References 1. Fu, Yanfang, et...Models with CRISPR/Cas9 Additional Resources on Addgene.org View Our CRISPR Guide Pages Find CRISPR Plasmids...
  8. With an Eye Towards the Future, We Look Back at the March for Science

    Type
    Blog Post
    Published
    Oct. 24, 2017, 1:54 p.m.
    ...the groundbreaking research happens, sometimes forgetting how to engage with friends, family, and the public...over in silence his very large one” (http://oll.libertyfund.org/titles/galilei-dialogues-concerning-two-new-sciences...I was also incredibly impressed with how well organized the march was, as well as the friendly atmosphere...
  9. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    Published
    April 17, 2018, 1:37 p.m.
    ...embryoid bodies which are compact balls of loosely organized tissues that express markers of all three germ...Central PMCID: 2956203 5. Robinton, Daisy A., and George Q. Daley. "The promise of induced pluripotent stem...the Stem Cell Field Additional Resources on Addgene.org Visit our Stem Cell Guide Page Find reprogramming...
  10. Addgene is Stopping Use of X (formerly Twitter)

    Type
    Blog Post
    Published
    Jan. 11, 2024, 2:15 p.m.
    ...decision allows us to remain aligned to our organizational values.  To preserve the knowledge, conversations...can reach out to us directly via email at help@addgene.org, and we invite you to follow us across our social...
  11. Trends in CRISPR and SynBio Technologies

    Type
    Blog Post
    Published
    Feb. 4, 2015, 3:58 p.m.
    ...forums such as http://www.genome-engineering.org and http://www.egenome.org." At the Keystone Meeting Eric...
  12. Technical Design of a Western Blot

    Type
    Blog Post
    Published
    Aug. 22, 2024, 1:15 p.m.
    ...gel-to-gel.  Ladder Whatever gel type you select, don’t forget to save a lane for your standards (ladder). You...AntibodyAntibodies 101: Secondary Antibodies Resources on Addgene.org Addgene's Western Blot Protocol Addgene's Western...Neuroscience Methods, 172(2), 250–254. https://doi.org/10.1016/j.jneumeth.2008.05.00...
  13. New FLIS Plasmid (April Fools 2022)

    Type
    Blog Post
    Published
    April 1, 2022, 1:15 p.m.
    ...size have allowed scientists to use it as a model organism to interrogate many scientific questions. But ...containing escaped flies. Nature 4:1-22. https://doi.org/4.01.2022/hafd...
  14. New Videos: Addgene Lab Tips

    Type
    Blog Post
    Published
    Feb. 15, 2018, 2:41 p.m.
    ...suggestions for future tips?  Send your ideas to blog@addgene.org.  Additional Resources on the Addgene Blog ...Science: We Share Science Additional Resources on Addgene.org Browse our Video Library Check out Our Protocol...
  15. Twenty Years of Sharing Science!

    Type
    Blog Post
    Published
    Jan. 8, 2024, 3:25 p.m.
    ...shipped 2,078 items to 764 scientists at 375 organizations in 21 different countries. It's safe to say ...protocols, and of course, the friendly folks at help@addgene.org! Fun fact! Did you know you can search for plasmids...
  16. SciComm with the Experts at Science in the News Part 1

    Type
    Blog Post
    Published
    March 16, 2017, 1:06 p.m.
    ...from Science in the News, a graduate student organization that works to promote science communication....non-scientists. These programs are all run and organized by graduate student researchers. That’s right,...
Showing: 361 - 380 of 825 results