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We narrowed to 602 results for: ace

Showing: 11 - 20 of 602 results

  2. What's New in CRISPR - December 2019

    Type
    Blog Post
    Published
    Dec. 17, 2019, 2:37 p.m.
    ...They called this editor Target-ACE (adenine and cytosine editor). Target-ACE exhibits increased editing efficiency...mammalian cells. Read the paper in bioRxiv Find Target-ACE   CRISPR/Cas9-based toolkit for epitope tagging ...this toolkit might be used in. Read the paper in ACS Synthetic Biology Find the CRISPRi toolkit SECURE...ribonucleoproteins for transient expression. They replaced the tetraloop of the sgRNA scaffold with a com...

  3. Hot Plasmids September 2018 - Optogenetics, RNA Localization, Fluorescent Protein, and Base-editing Tools

    Type
    Blog Post
    Published
    Sept. 18, 2018, 1:33 p.m.
    ...Chemical Biology 2018. PubMed: PMID 30220597. ACE system: A versatile in vivo fluorescent reporter ...monitors both APOBEC- and Cas9-mediated Editing (ACE) in real-time. The reporter constitutively expresses...fluorescence microscopy or flow cytometry. Using the ACE system, the Harris lab showed that human APOBEC3A-Cas9n-UGI...containing rat APOBEC1. Finally, they show that the ACE reporter can also quantify Cas9 cleavage, thus exhibiting...gene editing activities. Click here to find the ACE reporter plasmid! Martin et al., Nucleic Acids Research...

  5. Oh, The Places You Can Go: Careers in Science Communication - Writing for a Research Institute

    Type
    Blog Post
    Published
    Feb. 2, 2018, 3:20 p.m.
    ...College Park, but she quickly realized it wasn’t the place for her. “I went to grad school with the plan of...different directions is sometimes at odds with the space needed to be creative. “You need a lot of time to...Susan feels she was lucky to have been in the right place at the right time when she started her scicomm career...

  6. Four Base Editing Reporters to Monitor and Enrich Editing in Real-time

    Type
    Blog Post
    Published
    July 7, 2020, 1:15 p.m.
    ...mediated editing in real time The Harris lab created ACE, a reporter that monitors APOBEC (a family of cytidine...editing in real time (St. Martin et al., 2018). The ACE reporter is a bicistronic construct that consists... Figure 1: APOBEC- and Cas9-mediated editing (ACE) reporter in action. Image from St. Martin et al....expression to cells expressing eGFP and mCherry. ACE was used to identify cells that have been successfully...and APOBEC3B (St. Martin et al., 2018). TLDR: The ACE reporter from the Harris Lab relies on the restoration...for single base editing by APOBEC-Cas9 Though the ACE reporter quantifies APOBEC-Cas9 efficiency in real-time..., and luciferase. References Gaudelli NM, Komor AC, Rees HA, Packer MS, Badran AH, Bryson DI, Liu DR...

  7. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

    Type
    Blog Post
    Published
    March 3, 2020, 2:15 p.m.
    ...a red-shifted Ace-mScarlet, a fusion of a voltage-sensitive inhibitory rhodopsin (Ace2N) with the red ...Figure 2: Ace-mScarlet is a fusion between the voltage-sensitive inhibitory rhodopsin Ace2N and mScarlet...seven or eight amino acid linker between them. The Ace-mScarlet voltage indicator works like many FRET-opsin... an increase in the neuron’s membrane potential, Ace absorbance increases and results in higher quenching...blue-light-sensitive channelrhodopsins. Find the Ace-mScarlet voltage indicator plasmids at Addgene! Beck...mScarlet. Ace2N quenches a proportion of mScarlet's emission via FRET. Image from Beck and Gong, 2019. ...

  8. Transferable Skills Guide: Career Planning Resources

    Type
    Blog Post
    Published
    Nov. 14, 2017, 2 p.m.
    ...the ACS career fair is more industry-focused. For another industry-geared option, try the Biospace career...Biology (ASCB) or the American Chemical Society (ACS) are just 2 examples of organizations that hold large...best options are the Naturejobs Career Expo and the ACS career fair, which are larger meetings offering resources...Similar to a conference, job fairs bring you face-to-face with working professionals, with the added benefit...career events page. Biospace holds career events all over the country; sometimes several per month, giving... online career resources Once you’ve identified places you’d like to work, get online and take a look ...know, where they are, and who they know all in one place! This information can be critical to a focused and...

  9. R Bodies: Membrane-Rupturing Microscopic Tools

    Type
    Blog Post
    Published
    April 14, 2016, 2:30 p.m.
    ...spheroplasts to release encapsulated proteins. Video by ACS. For a more cartoony explanation of R-bodies, check... Breaks Membranes to Release Encapsulated Cargo. ACS Synth. Biol. (2016). doi:10.1021/acssynbio.5b00237...penetrate other barriers (like vasculature or extracellular matrices) in response to pH changes. Beyond...functionalized so that they adhere to specific surfaces, or they could be decorated with enzymes for use...

  10. Hot Plasmids and Viral Preps - January 2021

    Type
    Blog Post
    Published
    Jan. 5, 2021, 2:15 p.m.
    .... Created with BioRender.com In their recent ACS Synthetic Biology publication, Mary Dunlop’s lab ...cut in as little as 2 hours.  Sheets, MB, et al., ACS Synth Biol. 2020. https://pubs.acs.org/doi/10.1021...
Showing: 11 - 20 of 602 results