...large inserts (>7 kb)(Berg et al., 1989), direct repeats (Ribeiro et al., 2009), and at high copy numbers...plasmid recombination mediated by 28 bp direct repeats. Mol Biotechnol. 2008 Nov;40(3):252-60. doi: 10.1007...

...stop codon) and closed (including a stop codon) formats. The collection is compatible with both standard...kit (GGT) that streamlines the assembly of TALE repeats into a single-tube, 3 day process by utilizing ...hard-to-study genes in hard-to-study cell types. Natsume T, et al. Cell Rep. 2016. PubMed PMID: 27052166...

...desired target of RNAi is expressed as inverted repeats separated by a loop region that facilitates the... complementary to your gene target as inverted repeats (Figure 1). With this technique, multiple genes...

...DNA. Finally, in 1953, Franklin along with James Watson and Francis Crick resolved the DNA double helix...:3870–3879 . https://doi.org/10.1093/nar/gkf495 Watson JD, Crick FHC (2003) Reprint: Molecular Structure...

...green algae (Oesterhelt and Stoeckenius, 1971; Matsuno-Yagi and Mukohata, 1977; Foster et al., 1984; Harz...: W. H. Freeman, 2000. Bookshelf ID: NBK21668. Matsuno-Yagi, Akemi, and Yasuo Mukohata. "Two possible ...

...member of the Vancouver SciCATs. Follow her on twitter @Point_Mutation, and @SciCATSyvr. Additional Resources...

...contains a nontargeting spacer flanked by two CRISPR repeats. The spacer can be digested using BsaI, allowing...were to develop an array using 3 distinct spacer-repeats, you could easily create 7 unique arrays (e.g. ...

...  Monday: Thank you post (this is it!)  Tuesday: Stats roundup on Twitter Wednesday: 2022 Blue Flame Depositors...

...interview with Tom McFadden!   1. Rosalind Franklin vs. Watson & Crick - Science History Rap Battle A great example...

...Clustered Regularly Interspaced Short Palindromic Repeats: Sequences of DNA found in bacteria that allow ...

... Here's the schedule for the week: Monday: Fun stats and trivia begins! Tuesday: Depositor features (...

...Urbana-Champaign. The PUF proteins include eight modular repeats, each of which binds a consecutive base in its ...

...transitions at target sequences specified by gRNAs. Caveats, potential specificity issues, and other means ...

...every time you meet.  Seek feedback in multiple formats      Ask for input and opinions in meetings, via...

...that you are not interested in. You can use the carats in the datatable headers to sort alphabetically...

...by clustered regularly interspaced palindromic repeats (CRISPR)/Cas9 nuclease system in mammalian cells...

...last 10 years @Addgene Check out our impressive stats from 10 Years of Plasmid Sharing Or read more about...

...mentions that some of the team will be moving to flats with gardens this year and hopes that 2014 will ...

... the plant, similar to viral vectors’ terminal repeats. It’s a lot of information to fit into a single...

... will be adaptable to a wide variety of screen formats and contexts and continue to improve the accessibility...

...between the AAV genome’s two inverted terminal repeats (ITRs); but when recombinant AAV (rAAV) is made...

...Simplified, long-term single-molecule tracking Caveats of SunTag Very large size: a 24x scaffold fully...

...: "In fearful day, in raging night, With strong heats full, our souls ignite, When all seems lost in the...

...Clustered Regularly Interspaced Short Palindromic Repeats, in Pubmed returned 728 articles (3/12/2014). With...

...CRISPR (clustered regularly interspaced palindromic repeats) Cas (CRISPR associated genes) system. This popular...

...recombination often occurs between the long-terminal repeats (LTRs). After recombination, one resulting plasmid...

...possible new awardees, and we’re happy to share stats and strategies with tech transfer offices. Drop ...

...? It’s not a new idea, but it comes with a few caveats. The most common protein-only transduction strategy...

...functionality of the RNA scaffolds follows the rules of Watson-Crick base pairing, allowing for rational, straightforward...

...than in-person. You might miss out on the short chats between sessions or waiting for a session to start...

...) in an old Girl Scout Cookie factory with their cats, Harley and Lexi, and a clutter ofoutdoors-only ...

...which is depicted here: The heat-inducible Hsp (heatshock promoter) drives expression of the tumor-seeking...

...school teacher proudly boasted to me that he never eats “anything with chemicals in it,” leaving me to wonder...

...Experimenting in an Open Source Lab: from CRISPR to Cats Stem Cell Models for Disease & Open Science: Interview...

...translation dynamics, but they come with a few caveats. In both cases, creating a reporter mRNA requires...

...and simply called the repeated elements “direct repeats” and the unique stretches of DNA between them “...

...career paths? Ask other lab members how the advisor treats such inquiries. If you know you would like to head...

...Subcomponents: -U3 -R -U5 LTR; Long terminal repeats; U3-R-U5 regions found on either side of a retroviral...

...FLAG is eight amino acids, HA is nine), multiple repeats of the tag can recruit multiple frankenbodies to...

...10.3390/v3060677 Varanda C, Félix MDR, Campos MD, Materatski P. An Overview of the Application of Viruses ...