...PMID: 27175853. Pubmed Central PMCID: PMC4866822. 3. McDowell, Gary S., et al. "Shaping the Future of ...perspective from junior scientists."F1000Research 3 (2014). Pubmed PMID: 25653845. Pubmed Central PMCID...working group (National Academy of Sciences. “The Postdoctoral Experience Revisited” 2014 Appendix B p93-95...

...materials required for each [1, 2]. This protocol [3] provides, in exquisite detail, the various steps ...doi: 10.1038/nprot.2011.413. PubMed PMID: 22157972. 3. Britt JP, et al. Use of channelrhodopsin for activation...University of Michigan and he is currently a Postdoctoral Fellow in the lab of Mary Jeanne Kreek at the...

...not immunogenic, a necessity for in vivo studies; (3) is compatible with single gene KO and pooled screening... Find FITS plasmids here! Marty LaFleur is a Postdoctoral Fellow in Arlene Sharpe’s laboratory at Harvard...

...the impact of research" (2013) Scientific Reports 3, Article number: 1649 doi:10.1038/srep01649     ...been gaining ground. A study issued by Google in October entitled “Rise of the Rest: The Growing Impact ...

... kb at most - which means that a gene larger than 3 kb cannot be delivered using this vector. For example...emerges from disgrace to be the next big thing, again 3. Acland G. et al., 2001. Gene therapy restores vision...amaurosis. This is the first of what I, scientists, doctors and patients hope are many more clinical successes...

... Consulting firms deploy case teams, usually with 3-6 consultants, who work on analyzing the situation...the client to prioritize investments over the next 3, 5, or 10 years. Complicating these challenges is ...given therapeutic approach brings to patients, doctors, and insurance providers, and is crucial to how...

... of the optical fiber into the region of interest 3) Behavioral experimentation I’ll discuss some of the...thoroughly elsewhere (here and here for instance) [2], [3]. Karl Deisseroth of Stanford University , one of ...10.1146/annurev-neuro-061010-113817. PMID: 21692661.  3. Bernstein JG, Boyden ES. Optogenetic tools for analyzing...University of Michigan and he is currently a Postdoctoral Fellow in the lab of Mary Jeanne Kreek at the...

... enhanced sodium accumulation in their roots and (3) development of tools for data analysis/visualization...post was contributed by Magdalena Julkowska, a postdoctoral researcher at KAUST, Saudi Arabia. From the... submission to the subsequent journal.  As a postdoctoral researcher studying plant development and abiotic...

...one company, likely will need to move around every 3-6 years—a short and exciting ride Complicated matrix...recently, I have been hearing exit survey data from postdoctoral programs in the Boston area that demonstrate...Both explicit and implicit aspects of today’s postdoctoral training can directly interfere with a seamless...

... for a Successful Collaboration. PLoS Comput Biol 3:e44 . https://doi.org/10.1371/journal.pcbi.0030044...This post was contributed by Matteo Tardelli, a postdoctoral scientist at Weill Cornell Medicine. Discussing...blogger Matteo Tardelli! Matteo Tardelli, PhD is Postdoctoral Scientist at Weill Cornell Medicine in NYC and...

... to have either salaries raised or hours tracked, 3% of postdocs were at institutions focused only on ...of Information Act (FOIA) requests for their postdoctoral salary information as of December 1st. Salary...are an important step forward in helping the postdoctoral population continue to pursue academic work,...of the scientific enterprise by reducing the postdoctoral population which keeps growing despite a lack...

...that could be useful for a wide variety of reasons. 3) I would love to see more posts like this on the Addgene...deposited plasmids on this trip. Here’s to hoping that October is even more awesome than September! Tyler J. Ford...

... level should now be automatically adjusted every 3 years. In May 2016, Secretary of Labor Thomas Perez...exactly how postdoctoral salaries are changing. Transparent salary information for postdoctoral positions...institutions set their postdoctoral salary scales to the NRSA scale (Figure 21, National Postdoctoral Association...Stanford raised salary levels to $50,000, and as of October 1st the salary minimum was raised again to $51,...as having postdocs, representing ~85% of the postdoctoral workforce. Based on our communications with ...according to the NSF data, account for 18% of the postdoctoral workforce in the sciences. The Dana Farber Cancer...to have to get rid of postdocs; that current postdoctoral appointments may shorten in length; that postdoc...

...acceptable. Aims should be mechanistic, doable by 1-3 people in 1-5 years, and contribute to resolving the...contributed by Erik Snapp, the Director of Student and Postdoctoral Programs at the Janelia Research Campus of the...from Oregon Health Sciences University, did his postdoctoral fellowship with Jennifer Lippincott-Schwartz...and now serves as the Director of Student and Postdoctoral Programs at the Janelia Research Campus of the...

...to safely get back to our lab work. Figure 3: When a bison wanders into the campsite, leave your...Thermus aquaticus bacterium – was discovered at Octopus Hot Spring at YNP, and since then, many researchers...

...Brandon Sanderson "The Mistborn series is the first 3 books of a larger epic of fantasy novels. Set in a...not - to inspire you to read a little more this October. Science favorites Musicophilia: Tales of Music...

...100th birthday, as your mom will never forgive you… #3 Honor the work day and keep it holy Keep a regular...And, yes, your partner CAN take the kids to the doctor without your help. Your partner is equally responsible...eight-year-old daughter vehemently reminded her doctor that she was allergic to penicillin – I am much...

... scientists have certainly not lost site of that. 3. Stem cell science is hard, and expensive Dr. Yamanaka...differentiated cells simply by expressing four proteins Oct4, Sox2, Klf4, and cMyc (the so called OSKM factors...

...great tool for someone starting cell culture work. 3. NEB Tools by New England BioLabs In my lab we do ...Management Software Resources on Addgene.org Find Protoctols for Your Research Catch Up On Your Molecular ...

...while AAV-PHP.S is typically used at a dose between 3 x 1011 and 1 x 1012 vg*. Most systemic capsids are...transduction in New World monkeys   Figure 3: Evolutionary tree depicting the phylogenetic relationship...414 (2019). https://doi.org/10.1038/s41596-018-0097-3 More resources on the Addgene blog Viral Vectors 101...Sensory Ganglion Neurons Using AAV-PHP.S. eNeuro,9(3), ENEURO.0373-21.2022. https://doi.org/10.1523/ENEURO...Are Limited to C57BL/6J Mice. Molecular Therapy, 26(3), 664–668. https://doi.org/10.1016/j.ymthe.2018.01.018.... R., Flytzanis, N. C., Goeden, N., Christopher Octeau, J., Roxas, K. M., Chan, K. Y., Scherrer, J., Winchester...

... make it easy for your supervisor to support you. 3) Explain the problem and leave your emotions out of...the needs of other people. Some students and postdoctoral fellows are hesitant about expressing their ...

...PMID: 25599551. PubMed Central PMCID: PMC4344395. 3. Lavis, Luke D., and Ronald T. Raines. "Bright ideas... tag (Figure 1c). Click chemistry (e.g., transcyclooctene–tetrazine)– Nonnatural amino acids can be ...

...www.businessinsider.com/how-to-get-the-most-out-of-job-fairs-2014-3 https://www.forbes.com/sites/neilpatel/2015/05/21/... students today is that many graduate and post-doctoral programs lack any formal or mandatory professional...

...Government (2016). 2. CDC Information Pages on Antibiotics 3. WHO Antimicrobial Resistance Fact Sheet  Additional...www.youtube.com/watch?v=sx1uDYSfINA. When you go to the doctor, it will very likely take time to determine if ...

...biotechnology 16(7): 657–62. PubMed PMID: 9661200.  3. Cardin, Jessica A. et al. 2009. “Driving Fast-Spiking...guest blogger, Katrin Michel! Katrin Michel is a Postdoctoral Researcher at MIT and is fascinated by the question...

...the membrane is cut so each lane is its own piece (3). After incubation, blocking, and washing, the membrane...protein concentration decreases).     Figure 3: Testing the antibody’s dynamic range. A) The protein...resources with examples, like BioRad’s Western Blot Doctor page, or robust discussions, like ResearchGate’...

...or 1kb fragments are produced (see figure above). 3 Reasons The PSU Ladders Are Awesome Tools: They are... through the expression of transcription factors OCT3/4, SOX2, KLF4, and c-MYC is the established model...Addgene and include a variety of cMYC, KLF4, SOX2 and OCT4 DNA binding domain and transcription activation ...

...should be ~60°C (between 50 and 65, with no more than 3 degrees between the two Tms)  Aim for 50-60% GC content...Nucleic Acids Research, Volume 28, Issue 19, 1 October 2000, Pages 3752–3761, https://doi.org/10.1093/...