We narrowed to 70 results for: NIG
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TypeBlog PostPublishedJan. 5, 2023, 2:15 p.m....Every student about to graduate shares the same nightmare: the question “So, what comes next?” For those...
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Viral Vectors 101: Producing Your rAAV
TypeBlog PostPublishedJuly 16, 2024, 1:15 p.m..../10.1089/hgtb.2016.180 Korte, J., Mienert, J., Hennigs, J. K., & Körbelin, J. (2021). Inactivation of ... -
Quick Guide to Working with Drosophila Part 1: Getting Started with Flies
TypeBlog PostPublishedJuly 13, 2017, 2:30 p.m....throughout daytime. They mostly stop emerging at night. An incubator with a light cycle makes for very ... -
3D Printing Meets CRISPR Cas9
TypeBlog PostPublishedOct. 17, 2017, 12:55 p.m....while and you will wake up in the middle of the night dreaming about the conformational gymnastics that... -
Plasmids 101: Optimizing Plasmid Yields
TypeBlog PostPublishedMay 26, 2016, 2:30 p.m....fast enough for sufficient gas exchange in the overnight culture. Generally, large cultures in flasks may... -
INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons
TypeBlog PostPublishedDec. 7, 2020, 2:53 p.m....mini-transposon Transform plasmid(s) into cells and plate overnight Screen colonies phenotypically or by PCR/sequencing... -
Negative Can Be Positive: Open AAV Data with Addgene
TypeBlog PostPublishedSept. 6, 2022, 1:15 p.m....Sierra-Mercado, D., Robinson, J. O., Outram, S., Koenig, B. A., Pereira, S., McGuire, A. L., & Lázaro-Muñoz... -
MXS Chaining
TypeBlog PostPublishedFeb. 7, 2017, 3:30 p.m....875. Pubmed PMID: 13790780. 5. Shetty RP, Endy D, Knight TF. Engineering BioBrick vectors from BioBrick ... -
Plasmids 101: Colony PCR
TypeBlog PostPublishedMay 12, 2016, 2:30 p.m.... liquid cultures. Lastly, you can start small overnight liquid cultures with the clones you pick and only... -
Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor
TypeBlog PostPublishedJuly 23, 2019, 12:46 p.m.... stem cell antigen‐1: expression, function, and enigma." Stem cells 25.6 (2007): 1339-1347. PubMed PMID... -
Data Freedom: The Expansion of Data Sharing in Research Publications
TypeBlog PostPublishedAug. 5, 2014, 6:51 p.m....reported in PLOS publications. The policy was not an overnight revelation, rather it was the result of consultations... -
Transferable Skills Guide: Time Management
TypeBlog PostPublishedDec. 5, 2017, 1:52 p.m....the start of the day, writing your to-do list the night before will help keep you focused. For a real-world... -
CRISPR 101: Validating Your Genome Edit
TypeBlog PostPublishedNov. 3, 2022, 12:15 p.m....doi.org/10.1093/bioinformatics/btz867 Haeussler M, Schönig K, Eckert H, Eschstruth A, Mianné J, Renaud J-B... -
Antibodies 101: The Basics of Western Blotting
TypeBlog PostPublishedMarch 8, 2021, 2:41 p.m....broad range of protein sizes but takes hours to overnight. In contrast, a semi-dry transfer only requires... -
Viral Vectors 101: Optogenetic Tools
TypeBlog PostPublishedJune 27, 2023, 1:15 p.m....A., Ramakrishnan, C., Inoue, M., Shane, J. C., McKnight, D. J., Yoshizawa, S., Kato, H. E., Ganguli, S... -
Plasmids 101: Repressible Promoters
TypeBlog PostPublishedDec. 20, 2022, 2:15 p.m....PMCID: PMC49329. Triezenberg SJ, Kingsbury RC, McKnight SL. Functional dissection of VP16, the trans-activator... -
PiggyBac-ing Through the Genome Editing Field
TypeBlog PostPublishedMay 31, 2016, 3:30 p.m.... 2016. PubMed PMID: 27075976. 4. X. Li, E. R. Burnight, A. L. Cooney, N. Malani, T. Brady, J. D. Sander... -
Plasmids 101: Restriction Cloning
TypeBlog PostPublishedFeb. 18, 2016, 3:42 p.m....more colonies you will need to pick) and grow overnight cultures for DNA purification. The simplest purification... -
FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution
TypeBlog PostPublishedOct. 17, 2019, 12:28 p.m....where FLEx enters the scene. FLEx: A light when night is about you! The FLEx (for flip-excision) switch... -
With an Eye Towards the Future, We Look Back at the March for Science
TypeBlog PostPublishedOct. 24, 2017, 1:54 p.m....convince me to spend a Saturday in the tourist-trap nightmare that is Times Square. But I made the sacrifice...