We narrowed to 825 results for: org

...297 . https://doi.org/10.2307/1542146 Lazarus, J. E., Warr, A. R., Kuehl, C. J., Giorgio, R. T., Davis...sea anemone (Chan et al., 2006). Why do these organisms have chromoproteins? One reason may be that chromoproteins...symbiotic algae, during heat stress. For other organisms, like the sea anemone, scientists think that chromoproteins... for making plasmid constructs Resources on Addgene.org Find fluorescent proteins for your research ...xamachana. Marine Biology 94:423–430 . https://doi.org/10.1007/bf00428249 Chan MCY, Karasawa S, Mizuno...Biological Chemistry 281:37813–37819 . https://doi.org/10.1074/jbc.m606921200 Dove SG, Takabayashi M, ...and Environmental Microbiology, 85(16). https://doi.org/10.1128/aem.00990-19 Liljeruhm, J., Funk, S. K....

...Gene Therapy Methods, 23(1), Article 1. https://doi.org/10.1089/hgtb.2011.034 Benyamini, B., Esbin, M. ...Vectors [Preprint]. Molecular Biology. https://doi.org/10.1101/2023.03.20.533580 Goodwin, M. S., Croft...Molecular Neurodegeneration, 15(1), Article 1. https://doi.org/10.1186/s13024-020-00361-z Grimm, D., Kern, A.,... AAV-2. Gene Therapy, 6(7), Article 7. https://doi.org/10.1038/sj.gt.3300946 Howard, D. B., & Harvey, ...Gene Therapy Methods, 28(1), Article 1. https://doi.org/10.1089/hgtb.2016.180 Korte, J., Mienert, J., Hennigs...Gene Therapy, 32(13–14), Article 13–14. https://doi.org/10.1089/hum.2020.120 Lock, M., Alvira, M. R., Chen...Gene Therapy Methods, 25(2), Article 2. https://doi.org/10.1089/hgtb.2013.131 Lock, M., McGorray, S., Auricchio...

...Integrated in Parallel. Cell, 154(4), 914–927. https://doi.org/10.1016/J.CELL.2013.07.018 Anzalone, A. V., Randolph...donor DNA. Nature, 576(7785), 149–157. https://doi.org/10.1038/s41586-019-1711-4 Chen, P. J., & Liu, D...Nature Reviews Genetics, 24(3), Article 3. https://doi.org/10.1038/s41576-022-00541-1 Kim, H. K., Kim, Y.,...performance. Science Advances, 5(11), eaax9249. https://doi.org/10.1126/sciadv.aax9249 Koeppel, J., Weller, J.,...determinants. Nature Biotechnology 2023, 1–11. https://doi.org/10.1038/s41587-023-01678-y Li, X., Chen, W., Martin...epigenetic manipulation of prime editing. Cell. https://doi.org/10.1016/j.cell.2024.03.020 Mathis, N., Allam, A.... Nature Biotechnology, 41, 1151–1159. https://doi.org/10.1038/s41587-022-01613-7 Mathis, N., Allam, A...

...Vectors 101: Viral Applications Resources on addgene.org Addgene's Lentiviral Guide Most popular lentiviral...Microbiological Reviews, 56(3), 375–394. https://doi.org/10.1128/MR.56.3.375-394.1992. PubMed PMID: 1406488...and Perspectives. Viruses, 13(7), 1288. https://doi.org/10.3390/v13071288. PMID: 34372494. Prussin, A. ...Science & Technology Letters, 2(4), 84. https://doi.org/10.1021/ACS.ESTLETT.5B00050. PubMed PMID: 26225354...United States of America, 54(1), 137–144. https://doi.org/10.1073/PNAS.54.1.137. PubMed PMID: 4285422. Pubmed.... The EMBO Journal, 16(16), 5113–5122. https://doi.org/10.1093/EMBOJ/16.16.5113. PubMed PMID: 9305652....Journal of Virology, 14(5), 1132–1141. https://doi.org/10.1128/JVI.14.5.1132-1141.1974. PubMed PMID: 4372391...

...and stably maintain the genes they carry among organisms belonging to many different phylogenetic groups...bacteria and express proteins within different host organisms. pBBR1-based plasmids and plasmids in incompatibility...Biological Chemistry, 273(14), 8447–8453. https://doi.org/10.1074/jbc.273.14.8447  Jain, A., & Srivastava...FEMS Microbiology Letters, 348(2), 87–96. https://doi.org/10.1111/1574-6968.12241  Keen, N. T., Tamaki, S...Gram-negative bacteria. Gene, 70(1), 191–197. https://doi.org/10.1016/0378-1119(88)90117-5  Lale, R., Brautaset...Biology (Clifton, N.J.), 765, 327–343. https://doi.org/10.1007/978-1-61779-197-0_19  Meyer, R. (2009).... IncQ plasmids. Plasmid, 62(2), 57–70. https://doi.org/10.1016/j.plasmid.2009.05.001  Meyer, R., Figurski...

...methyl-CpG-binding protein 2. Nat Genet 23:185–188 . https://doi.org/10.1038/13810 Amir RE, Zoghbi HY (2000) Rett syndrome...correlations. Am J Med Genet 97:147–152 . https://doi.org/10.1002/1096-8628(200022)97:2<147::aid-ajmg6>3.0... Related Disorders. Cell 152:984–996 . https://doi.org/10.1016/j.cell.2013.01.038 Guy J, Gan J, Selfridge...Rett Syndrome. Science 315:1143–1147 . https://doi.org/10.1126/science.1138389 Le TTH, Tran NT, Dao TML...Pluripotent Stem Cells. Front Genet 10: . https://doi.org/10.3389/fgene.2019.00625 McGraw CM, Samaco RC, ... Requires MeCP2. Science 333:186–186 . https://doi.org/10.1126/science.1206593 Powers S, Miranda C, Dennys-Rivers...S51.002). Neurology 92 (15 Supplement) . http://n.neurology.org/content/92/15_Supplement/S51.002.abstract Sinnamon...

...was moved exactly as is from one organism to a distantly related organism, the gene may not express well...or intentional release of engineered organisms. “Is [the organism] engineered? Where did it come from?...this lies in the codon usage biases of different organisms. Different species have different preferences ...that it more closely resembles that of the host organism, it may express protein more efficiently. To classify...not much variation in GC content between many organisms and DNA synthesis companies often work within ...trend of sourcing genes from distantly related organisms. In 2006, less than 1% of the genes deposited ...the less likely it is transferred into another organism in the lab. Predicting lab-of-origin of engineered...

...fluorescent dyes have enabled the visualization of both organic tissues and cell cultures, opening biological interiors...cellular components. Some dyes target specific organelles like mitochondria, and different dyes can result...receptors. Scientific Reports, 10(1), 21842. https://doi.org/10.1038/s41598-020-78827-9  Breen, C. J., Raverdeau...ligand-binding assay. Scientific Reports, 6, 25769. https://doi.org/10.1038/srep25769  Grimm, J. B., & Lavis, L. D....labels. Nature Methods, 19(2), 149–158. https://doi.org/10.1038/s41592-021-01338-6  Grimm, J. B., Muthusamy...imaging. Nature Methods, 14(10), 987–994. https://doi.org/10.1038/nmeth.4403  Hayashi-Takanaka, Y., Stasevich...for live cell imaging. PLoS ONE, 9(9). https://doi.org/10.1371/journal.pone.0106271  Heinrichs, A. (2009...

... development. These are required for a growing organism to make the complicated transition from a clump...dynamic processes are largely absent in adult organisms, where tissues structures are more stable and ...movement from the bone marrow and other lymphoid organs to specific tissues where they can scan for any...is large scale cellular movement throughout an organism. Studying cell mobility within these complex...tools to directly follow immune cells in lymphoid organs. The development of cell sorters together with ...antibodies made it possible to track cells from one organ to another. Thus several labs were able to decipher...microscopy images of chemically coloured lymphoid organs did in the past. How do researchers track fluorescently...

... (New York, N.Y.), 339(6121), 819–823. https://doi.org/10.1126/science.1231143  Gaudelli, N. M., Komor... cleavage. Nature, 551(7681), 464–471. https://doi.org/10.1038/nature24644  Gaudelli, N. M., Lam, D. K... Nature Biotechnology, 38(7), 892–900. https://doi.org/10.1038/s41587-020-0491-6  Komor, A. C., Kim, Y... cleavage. Nature, 533(7603), 420–424. https://doi.org/10.1038/nature17946  Lander, E. S. (2016). The ...Heroes of CRISPR. Cell, 164(1), 18–28. https://doi.org/10.1016/j.cell.2015.12.041  Musunuru, K., Grandinette...Journal of Medicine, 392(22), 2235–2243. https://doi.org/10.1056/NEJMoa2504747  Rees, H. A., Wilson, C.,...editors. Science Advances, 5(5), eaax5717. https://doi.org/10.1126/sciadv.aax5717  Richter, M. F., Zhao, K...

...indispensable tools for manipulating cells and organisms. They can be used for a variety of research purposes...applications. Gene Therapy, 17(2), 150–157. https://doi.org/10.1038/gt.2009.135. PubMed PMID: 19847206 Desfarges...Viruses: Essential Agents of Life, 147–175. https://doi.org/10.1007/978-94-007-4899-6_7. PubMed Central PMCID... Nature Biotechnology, 31(9), 822–826. https://doi.org/10.1038/nbt.2623. PubMed PMID: 23792628 PubMed ...Clinical Investigation, 118(9), 3143–3150. https://doi.org/10.1172/JCI35798. PubMed PMID: 18688286 PubMed .... Journal of Virology, 70(2), 721–728. https://doi.org/10.1128/JVI.70.2.721-728.1996. PubMed PMID: 8551608...local hotspots. Cell, 110(4), 521–529. https://doi.org/10.1016/s0092-8674(02)00864-4. PubMed PMID: 12202041...

...interactions. Neuron, 113(16), 2599–2620.e7. https://doi.org/10.1016/j.neuron.2025.05.019    Unveiling the... transfer. Nature, 623(7986), 283–291. https://doi.org/10.1038/s41586-023-06537-z Hoover, G., et al. ...cancer metastasis. Nature, 644 252–262. https://doi.org/10.1038/s41586-025-09176-8 Spees, J. L., et al...Academy of Sciences USA, 103(5), 1283–1288. https://doi.org/10.1073/pnas.0510511103      Red calcium indicators...capabilities. bioRxiv 2024.12.16.628673. https://doi.org/10.1101/2024.12.16.628673 Zhou, S., Zhu, Q., Eom...resolution. PLoS Biol. 23(4): e3003048. https://doi.org/10.1371/journal.pbio.3003048   Micropeptide killswitch... diseases. Nature, 614(7948), 564–571. https://doi.org/10.1038/s41586-022-05682-1 Zhang, Y., et al. (2025...

...s next for prime editing? In addition to the smorgasbord of prime editing tools discussed here, many additional... Nature Biotechnology, 40(5), 731–740. https://doi.org/10.1038/s41587-021-01133-w Anzalone, A. V., Randolph...donor DNA. Nature, 576(7785), 149–157. https://doi.org/10.1038/s41586-019-1711-4 Chen, P. J., Hussmann...outcomes. Cell, 184(22), 5635-5652.e29. https://doi.org/10.1016/j.cell.2021.09.018 Choi, J., Chen, W., ... Nature Biotechnology, 40(2), 218–226. https://doi.org/10.1038/s41587-021-01025-z Doman, J. L., Pandey...editors. Cell, 186(18), 3983-4002.e26. https://doi.org/10.1016/j.cell.2023.07.039 Lin, Q., Jin, S., Zong... Nature Biotechnology, 39(8), 923–927. https://doi.org/10.1038/s41587-021-00868-w Nelson, J. W., Randolph...

...nonprofit or U.S. governmental research organizations, and organizations that require the H-1B employee to ...entity, or nonprofit research organizations or governmental research organizations. Since there is no numerical...Additionally, since most universities and non-profit organizations do not have the April 1 H-1B queue process or... or quota like industry, these organizations can be a good choice for individuals needing sponsorship.... in your field. Membership in a professional organization – this is not referring to one that you can ...program self-driving cars, tissue engineering for organ replacement).  In addition to presenting your expert...These candidates should also join professional organizations and attend the annual conferences – i.e. Society...

...the 4D Nucleome with CRISPR/Cas9 Resources at Addgene.org Read our CRISPR Guide to Epigenetics References...somatic enhancers. Nat Genet 52, 819–827. https://doi.org/10.1038/s41588-020-0639-9.  Feinberg A, Vogelstein...normal counterparts. Nature 301, 89–92. https://doi.org/10.1038/301089a0. Galonska C, Charlton J, Mattei...methyltransferase footprints. Nat Commun 9, 597. https://doi.org/10.1038/s41467-017-02708-5 Hrit J, Goodrich L, ...function in development. eLife 7:e34870. https://doi.org/10.7554/eLife.34870.  Kaas GA, Zhong C, Eason DE...Memory Formation. Neuron 79, 6 P1086-1093. https://doi.org/10.1016/j.neuron.2013.08.032.  Morita S, Noguchi...fusions. Nat Biotechnol 34, 1060–1065. https://doi.org/10.1038/nbt.3658. Sapozhnikov DM, Szyf M (2021)...

... Nucleic Acids Research 41:7429–7437 . https://doi.org/10.1093/nar/gkt520 Chavez A, Scheiman J, Vora S... programming. Nat Methods 12:326–328 . https://doi.org/10.1038/nmeth.3312 Chavez A, Tuttle M, Pruitt BW...multiple species. Nat Methods 13:563–567 . https://doi.org/10.1038/nmeth.3871 Konermann S, Brigham MD, Trevino...CRISPR-Cas9 complex. Nature 517:583–588 . https://doi.org/10.1038/nature14136 Perez-Pinera P, Kocak DD, Vockley...transcription factors. Nat Methods 10:973–976 . https://doi.org/10.1038/nmeth.2600 Qi LS, Larson MH, Gilbert LA... Gene Expression. Cell 152:1173–1183 . https://doi.org/10.1016/j.cell.2013.02.022 Sajwan S, Mannervik ...differ in target preference. Sci Rep 9: . https://doi.org/10.1038/s41598-019-54179-x Tanenbaum ME, Gilbert...

...different organizations and individuals, to identify ways of collaborating, and to organize events that...professional development (PD) workshops for teachers; organizing Congressional briefings in Washington DC; convening...Hollywood; and working directly with community organizations, libraries, youth groups, and faith institutions...studies (STS). I also worked with a number of organizations in public engagement efforts, including giving...middle and high school students. One of these organizations was pgEd. After obtaining my PhD and working...often “all hands on deck” to take care of the organizational logistics. Setting yourself on the path to ...audiences. Many universities or cities have organizations (often volunteer-based) that put on public lectures...

...multiplex immunolabeling of brain. eLife 8: . https://doi.org/10.7554/elife.43322 Baker M (2015) Reproducibility...on the antibodies. Nature 521:274–276 . https://doi.org/10.1038/521274a Begley CG, Ellis LM (2012) Raise... cancer research. Nature 483:531–533 . https://doi.org/10.1038/483531a Bradbury A, Plückthun A (2015) ...antibodies used in research. Nature 518:27–29 . https://doi.org/10.1038/518027a Bradbury AM, Plückthun A (2015)...recombinants once. Nature 520:295–295 . https://doi.org/10.1038/520295b Bradbury ARM, Trinklein ND, Thie...functional variable regions. mAbs 10:539–546 . https://doi.org/10.1080/19420862.2018.1445456 Crosnier C, Staudt...monoclonal antibodies. BMC Biology 8:76 . https://doi.org/10.1186/1741-7007-8-76 Greenfield, E.A. (Eds.),...

..."href":"https://datahub.addgene.org/","href_with_scheme":"https://datahub.addgene.org/","type":"EXTERNAL...Nature Reviews Immunology, 10(5), 301–316. https://doi.org/10.1038/nri2761 Chiu, M. L., Goulet, D. R., Teplyakov...Engineering therapeutics. Antibodies, 8(4), 55. https://doi.org/10.3390/antib8040055 Holliger, P., & Hudson, P....Nature Biotechnology, 23(9), 1126–1136. https://doi.org/10.1038/nbt1142 Kang, B. H., Lax, B. M., & Wittrup.... Methods in Molecular Biology, 29–62. https://doi.org/10.1007/978-1-0716-2285-8_2 McMahon, C., Baier,...Structural & Molecular Biology, 25(3), 289–296. https://doi.org/10.1038/s41594-018-0028-6 Packer, M. S., & Liu,...Nature Reviews Genetics, 16(7), 379–394. https://doi.org/10.1038/nrg3927 Wang, Y., Xue, P., Cao, M., Yu,...

... https://doi.org/10.1016/j.ymthe.2005.02.021 Zuk, P., Sanchez, C. E., Kostick, K., Torgerson, L., Muñoz..., 1–12. https://doi.org/10.3389/fnhum.2020.578687 Additional Resources on addgene.org  Download Addgene's...AAV PHP.B. Gene Therapy, 25(5), 392–400. https://doi.org/10.1038/s41434-018-0028-5 de Backer, M. W. A., ...Hypothalamus. Human Gene Therapy, 21(6), 673–682. https://doi.org/10.1089/hum.2009.169 Gruntman, A. M., Su, L., Su...Human Gene Therapy Methods, 26(2), 71–76. https://doi.org/10.1089/hgtb.2015.040 Initiative, F. B. (2019).... Gene Therapy Methods, 30(6), 214–225. https://doi.org/10.1089/hgtb.2019.105 Rumachik, N. G., Malaker,...Clinical Development, 18(September), 98–118. https://doi.org/10.1016/j.omtm.2020.05.018 Tedersoo, L., Küngas...

...CRISPR effector. Science 353:aaf5573 . https://doi.org/10.1126/science.aaf5573 Broughton JP, Deng X, Yu...detection of SARS-CoV-2. Nature Biotechnology. https://doi.org/10.1038/s41587-020-0513-4 Chen JS, Ma E, Harrington... DNase activity. Science 360:436–439 . https://doi.org/10.1126/science.aar6245 Gootenberg JS, Abudayyeh...CRISPR-Cas13a/C2c2. Science 356:438–442 . https://doi.org/10.1126/science.aam9321 Gootenberg JS, Abudayyeh...Cas12a, and Csm6. Science 360:439–444 . https://doi.org/10.1126/science.aaq0179 Myhrvold C, Freije CA, ...using CRISPR-Cas13. Science 360:444–448 . https://doi.org/10.1126/science.aas8836 Niewoehner O, Jinek M (...CRISPR-associated protein Csm6. RNA 22:318–329 . https://doi.org/10.1261/rna.054098.115 Shmakov S, Abudayyeh OO,...

...path.   Yeast as a model organism Yeast are single cell, eukaryotic organisms formally classified as fungi...know that yeast is a powerful eukaryotic model organism – its genome is easy to manipulate, it’s affordable...cerevisiae (budding). Yeast make for an excellent model organism for a few reasons: Affordable to culture and...studies Peroxisomes are small, membrane-bound organelles essential for sequestering certain enzymes and... methanol metabolism occurs in methylotrophic organisms. K. phaffii is an excellent model for peroxisome...et al., Komagataella phaffi as Emerging Model Organisms in Fundamental Research. Front. Microbiol. 11-...Resources on the Addgene Blog Five Popular Model Organisms Plasmids 101: Yeast Vectors Tips for Screening...

... Vectors. Microorganisms, 12(2), 310. https://doi.org/10.3390/microorganisms1202031038399714. PMID: 38399714...Vectors 101: Systemic Capsids More resource on Addgene.org Addgene's AVV guide AAV Viral Preps References...Expression. Hum Gene Ther, 31(3–4), 151–162. https://doi.org/10.1089/hum.2019.274. PMID: 31914802. Savy, A.,...Hum Gene Ther Methods, 28(5), 277–289. https://doi.org/10.1089/hgtb.2016.133. PMID: 28967288. Shitik, ... modification. Front Med, 10, 1106085. https://doi.org/10.3389/fmed.2023.1106085. PMID: 36817775. Wilmott...Hum Gene Ther Methods, 30(6), 206–213. https://doi.org/10.1089/hgtb.2019.276. PMID: 31752513. Zhou, Q....transgene expression. Sci Rep, 7(1), 5432. https://doi.org/10.1038/s41598-017-04054-4. PMID: 28710345....

...Nucleic Acids Research, 52(22):13931-13944. https://doi.org/10.1093/nar/gkae1066. Bryant, J., Herron, L., Doctor...gene expression via adaptamers. bioRxiv. https://doi.org/10.64898/2025.12.21.695777 Doman, J. L., Pandey...prime editors. Cell, 186(18), 3983–4002. https://doi.org/10.1016/j.cell.2023.07.039 Gupta, A., Gazzo, A....cancer. Nature Genetics, 57(6), 1452–1462. https://doi.org/10.1038/s41588-025-02187-1 Ortega, P., Bournique...catastrophe. Science Advances, 11(16), eadu0437. https://doi.org/10.1126/sciadv.adu0437 Pecori, R., Casati, B., ...tumor-specific neoantigens (editopes). bioRxiv. https://doi.org/10.1101/2023.03.16.532918 Pierce, S. E., Erwood...genome editing. Nature, 648(8092), 191–202. https://doi.org/10.1038/s41586-025-09732-2 Richter, M. F., Zhao...

...Collection are sorted by organism (see screenshot above). Don’t see your favorite organism listed? Help us serve...us know about other organisms we should include by emailing us at help@addgene.org. If you are looking...plasmids made in your lab, see www.addgene.org/deposit or email deposit@addgene.org for more information or ...to narrow your keyword search to one of these organisms. Help us continue to develop resources for your...like to get the word out about a particular microorganism, microbial technique, or microbial technology...

...with Addgene Senior Scientist, Matt Ferenc, to organize the CRISPR forum's most popular questions and ...Genes Genomes Genetics, 3(4), 657–664. https://doi.org/10.1534/g3.112.005439  Cong, L., Ran, F. A., Cox... systems. Science, 339(6121), 819–823. https://doi.org/10.1126/science.1231143  Elliott, B., Richardson...Molecular and Cellular Biology, 18(1), 93–101. https://doi.org/10.1128/mcb.18.1.93  Ran, F. A., Hsu, P. D., ... specificity. Cell, 154(6), 1380–1389. https://doi.org/10.1016/j.cell.2013.08.021  Ran, F. A., Hsu, ...system. Nature Protocols, 8(11), 2281–2308. https://doi.org/10.1038/nprot.2013.143 Sander, J. D., & Joung... Nature Biotechnology, 32(4), 347–355. https://doi.org/10.1038/nbt.2842  Soldner, F., Laganière, J., ...

... ACS Chemical Neuroscience 6:476–484 . https://doi.org/10.1021/cn500325v Goutaudier, R., Coizet, V., Carcenac...Ligands. Eneuro, 6(5), ENEURO.0171-19.2019. https://doi.org/10.1523/ENEURO.0171-19.2019Jendryka M, Palchaudhuri...chemogenetics in mice. Scientific Reports 9: https://doi.org/10.1038/s41598-019-41088-2 Magnus C, Lee P, Bonaventura...applications. Science 6436: eaav5282. https://science.sciencemag.org/content/364/6436/eaav5282.long Redfern ...mice. Nature Biotechnology 17:165–169 . https://doi.org/10.1038/6165 Roth BL (2016) DREADDs for Neuroscientists...Neuroscientists. Neuron 89:683–694 . https://doi.org/10.1016/j.neuron.2016.01.040 Saloman, J. L., Scheff, N... of Neuroscience, 36(42), 10769–10781. https://doi.org/10.1523/JNEUROSCI.3480-15.2016 Weston M, Kaserer...

...from organization to organization, so you’ll need to be familiar with your specific organization/department...-2 labs. When working with lentivirus, many organizations require or recommend disposable gowns over your...cabinet, even if it is not required by their organization. It’s always okay to add another level of protection..., 10% bleach, or 1% iodine are useful. Some organizations or cities may require a higher percentage of...cut. Following all sharps procedures for your organization will significantly reduce the risk of an accident...also need to familiarize yourself with your organization’s reporting mechanisms before you start an experiment.... Happy transducing! Resources Resources on Addgene.org Addgene viral services Addgene Biosafety Guide...

...CRISPR-Cas systems. A full list of Acr proteins organized by the type of CRISPR-Cas system they block can...anti-CRISPR protein. Nature 546:436–439 . https://doi.org/10.1038/nature22377 Harrington LB, Doxzen KW, Ma... CRISPR-Cas9. Cell 170:1224-1233.e15 . https://doi.org/10.1016/j.cell.2017.07.037 Li C, Psatha N, Gil ...Methods & Clinical Development 9:390–401 . https://doi.org/10.1016/j.omtm.2018.04.008 Marino ND, Pinilla-Redondo...technologies. Nat Methods 17:471–479 . https://doi.org/10.1038/s41592-020-0771-6 Mayo-Muñoz D, He F, Jørgensen...islandicus Rod-Shaped Virus 2. Viruses 10:695 . https://doi.org/10.3390/v10120695 Pawluk A, Amrani N, Zhang Y, ...for CRISPR-Cas9. Cell 167:1829-1838.e9 . https://doi.org/10.1016/j.cell.2016.11.017 Shin J, Jiang F, Liu...

...}{"url":{"content_id":null,"href":"https://www.addgene.org/crispr/empty-grna-vectors/","type":"EXTERNAL...prime editors. Nat Biotechnol 38:824–844 . https://doi.org/10.1038/s41587-020-0561-9 Dempster JM, Pacini C...dependency data sets. Nat Commun 10: . https://doi.org/10.1038/s41467-019-13805-y Doench JG (2017) Am ...genetic screens. Nat Rev Genet 19:67–80 . https://doi.org/10.1038/nrg.2017.97 Hanna RE, Doench JG (2020) ...experiments. Nat Biotechnol 38:813–823 . https://doi.org/10.1038/s41587-020-0490-7 Radzisheuskaya A, Shlyueva...repression. Nucleic Acids Res 44:e141–e141 . https://doi.org/10.1093/nar/gkw583 Rees HA, Liu DR (2018) Base ...living cells. Nat Rev Genet 19:770–788 . https://doi.org/10.1038/s41576-018-0059-1 Veres A, Gosis BS, Ding...

...to deliver a standard AdV vector to the targeted organs or tissues with Cas9 and a gRNA designed to target...Genome Editing. Circ Res 115:488–492 . https://doi.org/10.1161/circresaha.115.304351 Holkers M, Maggio... nucleases. Nat Methods 11:1051–1057 . https://doi.org/10.1038/nmeth.3075 Lukashev AN, Zamyatnin AA Jr...perspectives. Biochemistry Moscow 81:700–708 . https://doi.org/10.1134/s0006297916070063 Maddalo D, Manchado E...CRISPR/Cas9 system. Nature 516:423–427 . https://doi.org/10.1038/nature13902 Maggio I, Holkers M, Liu J,...diverse array of human cells. Sci Rep 4: . https://doi.org/10.1038/srep05105 Stephens CJ, Lauron EJ, Kashentseva...Journal of Controlled Release 298:128–141 . https://doi.org/10.1016/j.jconrel.2019.02.009 Wang D, Mou H, Li...

...CRISPR–Cas system. Nature 577:271–274 . https://doi.org/10.1038/s41586-019-1849-0 Klompe SE, Vo PLH, Halpin-Healy... DNA integration. Nature 571:219–225 . https://doi.org/10.1038/s41586-019-1323-z Peters JE, Makarova KS...Proc Natl Acad Sci USA 114:E7358–E7366 . https://doi.org/10.1073/pnas.1709035114 Rubin BE, Diamond S, Cress...Within Microbial Communities. bioRxiv https://www.biorxiv.org/content/10.1101/2020.07.17.209189v2 Strecker...CRISPR-associated transposases. Science 365:48–53 . https://doi.org/10.1126/science.aax9181 Strecker J, Ladha A, Makarova... transposases.” Science 368:eabb2920 . https://doi.org/10.1126/science.abb2920 Vo PLH, Ronda C, Klompe...bacterial genome engineering. Nat Biotechnol. https://doi.org/10.1038/s41587-020-00745-y Zhang Y, Sun X, Wang...

...Why have zebrafish become such a popular model organism? Zebrafish (Figure 1) were initially recognized...reasons that zebrafish have been embraced as a model organism, I’ll outline a few of the available genetic resources...reason for the success of zebrafish as a model organism is its amenability to genetic manipulation. Tools...Europe, and China. In addition, the Zebrafish Model Organism Database ZFIN actively curates information on ...Generating zebrafish transgenics Creating transgenic organisms by introducing foreign DNA into the genome is ...fertilized zebrafish zygote transforms into an organism with a clearly established body plan including...pinhead—contribute to the “sculpting” of tissues and organs during embryo development. Single cell sequencing-based...

...blog posts about plasmid cloning Resources on Addgene.org Download the Plasmids 101 eBook  Browse plasmids...low-copy number plasmids. Plasmid 80:54–62 . https://doi.org/10.1016/j.plasmid.2015.03.007 Pubmed PMID: 25889267... Molecular Microbiology 56:1430–1440 . https://doi.org/10.1111/j.1365-2958.2005.04643.x Pubmed PMID: 15916596...plasmids in vivo. PLOS ONE 12:e0172913 . https://doi.org/10.1371/journal.pone.0172913 Pubmed PMID: 28245276...Plasmid Incompatibility. PLoS ONE 7:e29875 . https://doi.org/10.1371/journal.pone.0029875 Pubmed PMID: 22253811...Letters in Applied Microbiology 47:235–240 . https://doi.org/10.1111/j.1472-765x.2008.02426.x Pubmed PMID: 19241516...Opinion in Structural Biology 22:72–79 . https://doi.org/10.1016/j.sbi.2011.11.001 Pubmed PMID: 22153351...

... Resources and references More resources on addgene.org  Addgene's CRISPR Guide Addgene's CRISPR 101 ...Reviews Microbiology, 20(11), 641–656. https://doi.org/10.1038/s41579-022-00739-4 References Bravo, J....CRISPR–Cas9. Nature, 603(7900), 343–347. https://doi.org/10.1038/s41586-022-04470-1 Cofsky, J. C., Soczek...Structural & Molecular Biology, 29(4), 395–402. https://doi.org/10.1038/s41594-022-00756-0 Jiang, F., & Doudna,...Annual Review of Biophysics, 46, 505–529. https://doi.org/10.1146/annurev-biophys-062215-010822 Nishimasu...and Target DNA. Cell, 156(5), 935–949. https://doi.org/10.1016/j.cell.2014.02.001 Osuka, S., Isomura, ...CRISPR–Cas9. The EMBO Journal, 37(10), e96941. https://doi.org/10.15252/embj.201796941 Pattanayak, V., Lin, S....

... the effects of the drug on the actual cells or organ system. This allows scientists to get a more definitive...Patients. Frontiers in Immunology, 13. https://www.frontiersin.org/articles/10.3389/fimmu.2022.885672Piché, ...Assessment (pp. 519–542). Academic Press. https://doi.org/10.1016/B978-0-12-417144-2.00027-5van Brummelen...Challenges. The oncologist, 21(10), 1260–1268. https://doi.org/10.1634/theoncologist.2016-0061WHO. International...Frontiers in drug discovery, 2, 952326. https://doi.org/10.3389/fddsv.2022.952326Joubert, M. K., Deshpande...Biotherapeutics. PloS one, 11(8), e0159328. https://doi.org/10.1371/journal.pone.0159328Groell, F., Jordan,...Pharmazeutische Verfahrenstechnik e.V, 130, 128–142. https://doi.org/10.1016/j.ejpb.2018.06.008Lu, RM., Hwang, YC., ...

...investigate the susceptibility of cells from different organisms to SARS-CoV-2 (Chu et al., 2020). An additional...mechanisms to maintain and grow these critical organizations should be a fundamental part of the scientific... study. The Lancet Microbe 1:e14–e23 . https://doi.org/10.1016/s2666-5247(20)30004-5 McCray PB Jr, Pewe...Syndrome Coronavirus. JVI 81:813–821 . https://doi.org/10.1128/jvi.02012-06 Ou X, Liu Y, Lei X, Li P, ...cross-reactivity with SARS-CoV. Nat Commun 11: . https://doi.org/10.1038/s41467-020-15562-9 Subbarao K, Roberts ...SARS? Trends in Microbiology 14:299–303 . https://doi.org/10.1016/j.tim.2006.05.007 (2020) Open for outbreaks...outbreaks. Nat Biotechnol 38:377–377. https://doi.org/10.1038/s41587-020-0499-y Additional resources on the...

...https://doi.org/10.7554/eLife.64631  Goertsen D, et al. Nature Neuroscience. 2022. https://doi.org/10.1038...proteomic mapping in both cell culture and whole organisms (see our 2019 overview). Until recently, the approach...dedicated transgenic line. Using zebrafish as a model organism, the Parton Lab recently created a flexible system...Given that the liver is an immunologically active organ, this advance is likely to afford the ability to...Goertsen D, et al. Nature Neuroscience. 2022. https://doi.org/10.1038/s41593-021-00969-4.   dCas9-SSAP with ...here! Wang et al. Nat Cell Biol. 2022. https://doi.org/10.1038/s41556-021-00836-1 Visual barcodes for...here! Kaufman, et al. 2022. Nature Comm.https://doi.org/10.1038/s41467-022-30008-0      ...

...paper or preparing a presentation, it's easy to forget that you have a much bigger job than merely telling... at Addgene, ComSciCon, and many more great organizations. At ComSciCon, our primary mission is to strengthen...respective fields. To help attendees launch new organizations that will extend opportunities to practice science...education initiatives like BiteScis (https://bitescis.org/) and amazing graduate student writing collaboratives...collaboratives like Oceanbites (https://oceanbites.org/) have already grown out of our workshops. If you'... create them.  Seek out collaborations with organizations that practice science communication or start...Facebook, Instragram, or send an email to info@comscicon.org. Many thanks to our guest blogger, Nathan Sanders...

... vector you are using. Contact Addgene (help@addgene.org), or the supplier of your vector, to obtain this...in the Mouse Brain. PLoS ONE 8:e76310. https://doi.org/10.1371/journal.pone.0076310 Burger C, Gorbatyuk... System. Molecular Therapy 10:302–317. https://doi.org/10.1016/j.ymthe.2004.05.024 Dudek AM, Pillay S,...Independent of AAV Receptor. J Virol 92. https://doi.org/10.1128/jvi.02213-17 Fischer KB, Collins HK, Callaway...Proc Natl Acad Sci USA 116:27001–27010. https://doi.org/10.1073/pnas.1915974116 Guerin K, Rego M, Bourges...Extracts. Human Gene Therapy 31:664–678. https://doi.org/10.1089/hum.2019.277 Lavin TK, Jin L, Lea NE, Wickersham...Concentrations. Front Synaptic Neurosci 12. https://doi.org/10.3389/fnsyn.2020.00006 O’Carroll SJ, Cook WH,...

...–9. https://doi.org/10.1038/s41592-023-02085-6. PMID: 38036853. Preprint: https://doi.org/10.21203/rs.3...–4. https://doi.org/10.1038/s41587-023-02018-w. PMID: 38081970. Preprint: https://doi.org/10.21203/rs.3...Biotechnology, 10.1038/s41587-023-01756-1. https://doi.org/10.1038/s41587-023-01756-1. PMID: 37095348. ...from StayGold. ResearchSquare (Preprint). https://doi.org/10.21203/rs.3.rs-3188559/v1.  Neurogenetic alchemy... Nanotechnology, 18, 1241–1251 (2023). https://doi.org/10.1038/s41565-023-01419-x. PMID: 37430038. Endothelial-specific...primates. Nature Communications, 14(1), 3345. https://doi.org/10.1038/s41467-023-38582-7. PMID: 37291094.   ...

...steps. Molecular Cell, 70(3), 385-394.e3. https://doi.org/10.1016/j.molcel.2018.03.031 Xiao, Y., Luo, M.,... Cascade and Cas3. Science, 361(6397). https://doi.org/10.1126/science.aat0839 Yoshimi, K., & Mashimo,... Gene and Genome Editing, 3–4, 100013. https://doi.org/10.1016/j.ggedit.2022.100013 O’Brien, R. E., Bravo...Cascade. Molecular Cell, 83(5), 746-758.e5. https://doi.org/10.1016/j.molcel.2023.01.024 Morisaka, H., Yoshimi...human cells. Nature Communications, 10(1). https://doi.org/10.1038/s41467-019-13226-x Cameron, P., Coons, ...Nature Biotechnology, 37(12), 1471–1477. https://doi.org/10.1038/s41587-019-0310-0 Makarova, K. S., Wolf...Reviews Microbiology, 13(11), 722–736. https://doi.org/10.1038/nrmicro3569 Additional resources on the...

...About Developing Lab Management Software    Organizing and sharing your updated protocols So that’s ... mobile) tools are available for scientists to organize and share their scientific protocols. Here are...lab protocols online Addgene Website: http://www.addgene.org/ If you’re working with plasmids and you decide...submit an updated protocol? Just email us at help@addgene.org and we will be happy to upload your newest resources...Supplemental Document”: gRNA_Cloning Vector (deposited by George Church’s lab). Addgene has also cataloged a variety... Protocol Online Website: http://www.protocol-online.org/ Protocol Online is one of the early protocol...streamline your lab management and you want the works: organizing reagent storage and inventory, managing orders...

... such resources in less genetically tractable organisms lags behind. BiFC can also be used to screen for...interactions in live Drosophila. eLife 7. https://doi.org/10.7554/elife.38853 Hu C-D, Chinenov Y, Kerppola...Complementation. Molecular Cell 9:789–798. https://doi.org/10.1016/s1097-2765(02)00496-3 Kerppola TK (2006... living cells. Nat Protoc 1:1278–1286. https://doi.org/10.1038/nprot.2006.201 Kerppola TK (2013) Bimolecular...Spring Harb Protoc 2013:pdb.prot076497. https://doi.org/10.1101/pdb.prot076497 Kim Y, Jung JP, Pack C-G...Saccharomyces cerevisiae. Genome Res 29:135–145. https://doi.org/10.1101/gr.231860.117 Kodama Y, Hu C-D (2012) Bimolecular...update and future perspectives. Biotech 53. https://doi.org/10.2144/000113943 Additional resources on the Addgene...

... priority and have organized many events over the years. To keep the organizational side of things from...Use it! 8. Organize If you’re doing an event that requires people to be in groups, organize your troops...event! Addgene company culture At Addgene we organically developed the philosophy of  “work hard, play... in getting the fun started. I’m aware that  “organizing the fun” sounds a little neurotic, but hey- sometimes...posters, megaphone, or your company’s intranet. Don’t forget about the power of simple word of mouth - be sure... her operational responsibilities, she enjoys organizing team building events at Addgene and cultivating...

...proteins. Nature Communications, 12(1), 1384. https://doi.org/10.1038/s41467-021-21559-9 Huang, M. E., Qin, Y.... Nature Cell Biology, 26(2), 294–304. https://doi.org/10.1038/s41556-023-01342-2 Koblan, L. W., Arbab...Nature Biotechnology, 39(11), 1414–1425. https://doi.org/10.1038/s41587-021-00938-z Koblan, L. W., Doman... Nature Biotechnology, 36(9), 843–846. https://doi.org/10.1038/nbt.4172 Kurt, I. C., Zhou, R., Iyer, S...cells. Nature Biotechnology, 39(1), 41–46. https://doi.org/10.1038/s41587-020-0609-x Yang, C., Dong, X., Ma...Baden-Wurttemberg, Germany), 9(26), e2202957. https://doi.org/10.1002/advs.202202957 Yuan, T., Yan, N., Fei, ...methods. Nature Communications, 12(1), 4902. https://doi.org/10.1038/s41467-021-25217-y Zhao, D., Li, J., Li...

... Nature Biotechnology, 42(4), 638–650. https://doi.org/10.1038/s41587-023-01821-9 He, Y., Zhou, X., Chang.... Molecular Cell, 84(7), 1257-1270.e6. https://doi.org/10.1016/j.molcel.2024.01.021 Tong, H., Liu, N.,...National Science Review, 10(8), nwad143. https://doi.org/10.1093/nsr/nwad143 Tong, H., Wang, H., Wang, X...glycosylase. Nature Communications, 15(1), 4897. https://doi.org/10.1038/s41467-024-49343-5 Tong, H., Wang, X., ...Nature Biotechnology, 41(8), 1080–1084. https://doi.org/10.1038/s41587-022-01595-6 Ye, L., Zhao, D., Li...Nature Biotechnology, 42(10), 1538–1547. https://doi.org/10.1038/s41587-023-02050-w Yi, Z., Zhang, X., Wei...glycosylase. Nature Communications, 15(1), 6397. https://doi.org/10.1038/s41467-024-50012-w Additional Resources...

...screens. bioRxiv 2024.03.17.585235; doi: https://doi.org/10.1101/2024.03.17.585235.   Improved prime editor...RNA-binding protein. Nature. 2024 Apr 3. doi: https://doi.org/10.1038/s41586-024-07259-6. PMID: 38570691. Antibodies...monoclonal antibody. Virology. 558, 28–37. https://doi.org/10.1016/j.virol.2021.01.003. PMID: 33714753. ...emitting at 525 nm. Image reused from https://www.janelia.org/open-science/voltron-and-positron.  Both...2023) Neuron, 111(10), 1547–1563.e9. doi: https://doi.org/10.1016/j.neuron.2023.03.009. PMID: 37015225.  ...Virus. bioRxiv 2023.12.12.571321; doi: https://doi.org/10.1101/2023.12.12.571321.  Revolutionizing Gene...vitro and in vivo, including in human retinal organoids and live mice. Importantly, REVeRT avoids the ...

...critical in the business world. Companies and organizations that avoid all conflict can fall into “groupthink...individuals involved, and also for the labs, organizations, or businesses as a whole. When I’m attempting... sharing gets more challenging the bigger an organization gets, and creating new avenues of communication...should be communicated is necessary in a large organization, because confirming that every person has read...attempting to resolve conflicts within a given organization, it can help tremendously to have as much context... solutions that benefit themselves and their organization. Resolving conflicts is great, but avoiding ...Thomas-Kilmann Conflict Model, courtesy of https://managementweekly.org/thomas-kilmann-conflict-resolution-model...

... (XFP) of different colors to label an organ or entire organism. Because each cell can have multiple copies...The ability to track single cell dynamics at the organism level has been made possible by tools that allow.... Genes encoding the fluorescent proteins are organized in tandem with a single promoter, but only the...developed for tracking various cell types and organisms and for improving the level of detail possible...that allow for multicolor labeling of an entire organism or a specific tissue. XFPs are introduced using...systems allow single cell dynamics in a variety of organisms to be observed in more detail than ever before...fluorescent proteins topic page Resources on Addgene.org Browse Addgene’s fluorescent protein collection...