We narrowed to 427 results for: RAP

...Future of rAAV gene therapy: Platform for RNAi, Gene Editing and Beyond." Human Gene Therapy ja (2017). PubMed...being made to make them even more useful for therapeutic purposes. The University of Pennsylvania and ...These vectors can be used to improve targeted therapies for the eye, skeletal muscles, and the central...Cas9 but have the potential to deliver other therapeutic molecules such as microRNAs (miRNAs), short hairpin...insertional mutagenesis, and cancer." Molecular Therapy 15.10 (2007): 1740-1743. PubMed PMID: 17882145....tropism in mice after systemic injection." Molecular Therapy 16.6 (2008): 1073-1080. PubMed PMID: 18414476. ...anterograde and retrograde axonal transport." Human gene therapy 25.8 (2014): 705-720. PubMed PMID: 24694006. PubMed...

...Monoclonal antibody drugs are popular therapeutics for a plethora of disease conditions, from cancer ...response. T cell epitopes are found in either the therapeutic antibody itself or as impurities in the formulation... algorithm to evaluate the immunogenicity of therapeutics in silico. These algorithms are trained on vast...algorithms fail to capture. In vitro evaluation of ‌therapeutic immunogenicity is typically performed using traditional...In-vitro models for immunogenicity prediction of therapeutic antibodies. Used under CC BY fromGroell et.al... et.al, 2018). Treating these cells with the therapeutic antibody can help us assess the effects of the...researchers to develop safer and more effective mAb therapeutics. The in silico method of immunogenicity assessment...

...Using rAAV6. Molecular Therapy 19:36–45 . https://doi.org/10.1038/mt.2010.205 Trapani I, Colella P, Sommella...sequence derived from the filamentous phage F1 (Trapani et al., 2014). Drawbacks: The choice of homology...need to be optimized for each unique application (Trapani et al., 2015).  Expression of smaller fragments... of Adeno-Associated Virus Capsids. Human Gene Therapy Methods 27:1–12 . https://doi.org/10.1089/hgtb.2015.140...Capacity in a Transgene-independent Manner. Molecular Therapy 16:124–130 . https://doi.org/10.1038/sj.mt.6300322...Reassembly of Adeno-associated Virus. Molecular Therapy 18:6–8 . https://doi.org/10.1038/mt.2009.280 Hirsch...DNA-PKcs-independent, Rad51C-dependent Repair Pathway. Molecular Therapy 21:2205–2216 . https://doi.org/10.1038/mt.2013.184...

...immunology, cancer-immune cell interactions, cancer immunotherapy, as well as new approaches to reprogramming ...reprogramming tumor-associated inflammation for therapeutic benefit ”as stated by the organizers. My overall impression... discussed the effects of starvation on cancer therapy. Findings in previous animal studies suggested ...immunosuppressive reaction in order to improve therapeutic outcomes.  If you are studying inflammation and...to help rid us of cancer in combination with chemotherapy. To dive deeper into this field read our recent...doctoral candidate at Binghamton University! Cancer therapeutics The so called field of Immuno-Oncology, a sizzling... hot topic, includes studies on novel cancer therapeutics such as  (Chimeric Antigen Receptors) CAR T ...

...attractive vectors for research applications and gene therapy: they can be produced at high titers, can accommodate...the Adv genome’s utility in research and its therapeutic potential. Note: constructs discussed are based...Potential applications for gene and cell-based therapies in the new era of personalized medicine (2017)...Gutless AdV have generated high interest for gene therapy due their increased cloning capacity (up to 36 ...20 additional days at which time the cells are scraped, and lysed by multiple freeze-thaw cycles. The ...References 1. Luo, Jinyong, et al. "A protocol for rapid generation of recombinant adenoviruses using the... the AdEasy System. 3. Jager, Lorenz, et al. "A rapid protocol for construction and production of high-capacity...

... vectors in cancer immunotherapy. Oldham RA, Berinstein EM, Medin JA. Immunotherapy. 2015;7(3):271-84.... and they’ve also had success in clinical gene therapy applications. Read on to learn more about the current...2nd generation lentiviral packaging system The graphic below shows how the lentiviral genome was condensed...interested in developing these vectors for gene therapy. In 2003, VIRxSYS began the first clinical trial...of 2013, 65 patients had been treated with this therapy, which lowered viral load and did not cause severe...Lentiviral vectors are promising agents for cancer immunotherapy, but this research is still in the pre-clinical...These safer vectors have become standard in gene therapy. Researchers have also developed integrase-deficient...

...gene therapy have also been in the limelight. The FDA approved the first oncolytic viral therapy in 2015... science research all the way to clinical gene therapy. But how can you use these amazing tools in your...libraries makes them the ideal vector for the job. Therapeutic applications Vaccine development Attenuated and...being exploited as delivery mechanisms for gene therapy. The tractability of AAV, low immunogenicity, and...for human patients. Multiple viral-based gene therapies have already been approved by the FDA, and this...you are interested in basic science or clinical therapy—viruses could further your efforts. And Addgene... et al. Viral vector platforms within the gene therapy landscape. Sig Transduct Target Ther 6, 53 (2021...

...benefits antibody-based therapeutics. Whether antibodies are used directly as therapeutic agents or serve as...antibody engineering, to improve reagents and therapeutics. What is antibody engineering (and why do we...evolution Directed evolution is a method employed to rapidly change a protein towards a specific goal, like ...allowed us to develop more specific antibodies for therapies and improve their delivery methods to various ...end_module_attribute %}{% module_attribute "wrap_field_tag" is_json="true" %}{% raw %}"div"{% endraw %...engineering to develop antibody-based cancer therapeutics.    References and Resources References Chan...Chan, A. C., & Carter, P. J. (2010). Therapeutic antibodies for autoimmunity and inflammation. Nature Reviews...

...inverted terminal repeat sequences. Human Gene Therapy Methods, 23(1), Article 1. https://doi.org/10.1089... Utilizing minimally purified secreted rAAV for rapid and cost-effective manipulation of gene expression...can limit production of recombinant AAV-2. Gene Therapy, 6(7), Article 7. https://doi.org/10.1038/sj.gt...Inactivation of AAV Serotype 1 Vectors. Human Gene Therapy Methods, 28(1), Article 1. https://doi.org/10.1089...Vectors by Oxidant-Based Disinfectants. Human Gene Therapy, 32(13–14), Article 13–14. https://doi.org/10.1089...genome titers by droplet digital PCR. Human Gene Therapy Methods, 25(2), Article 2. https://doi.org/10.1089...type 2 Reference Standard Material. Human Gene Therapy, 21(10), Article 10. https://doi.org/10.1089/hum...

...Some things cannot be photographed or need text to properly contextualize. Graphics/cartoons (no words...be a hard sell!) Digital platforms often have demographic data of their users readily accessible via web...have a solid understanding of their audience demographics. Do your research so you can go to where your...depending on factors like socioeconomic status and geographic location. For example, not all social media platforms... biology are often more suited for cartoons or graphics for visuals. However, it’s much more challenging...academic to informal writing can difficult.  Photography Medium (high for microscopy) Visually compelling...may need text for audience interpretation. Infographics Low Combines both words and images; especially...

...versus the response and use that information to extrapolate the concentration of an unknown sample. For example...standard curve? To create a standard curve, you graph the measured response of the serially diluted standard...absorbance is plotted on the y-axis. With the help of graphing software, you then generate a trendline for the...sigmoidal. Once the trendline is calculated, you can extrapolate the concentration of the unknown sample based...sample is determined by using the trendline to extrapolate its x value, 0.963 mg/mL, from its y value, 0.651...the quality of a standard curve scientists use graphing software to calculate the data’s coefficient of..., manufacturers often recommend using advanced graphing software designed to plot complex trendlines. ...

...biotinylated. Read more about RapID in this blog! Find plasmids for RapID EXCELL: Proximity labeling for...APEX: engineered ascorbate peroxidase that can rapidly biotin label proteins A year after BioID became...  Find the APEX2 plasmids! TurboID and minturbo: Rapid and non-toxic proximity labeling  TurboID and miniTurbo...hours to label proteins and APEX2, although it can rapidly label proteins, requires H2O2 which can be toxic...-BioID blog post. Find plasmids for Split-BioID RaPID: RNA-protein interaction detection So far, we’ve...that can regulate multiple biological functions. RapID (RNA-protein interaction detection) uses proximity...interactions (Ramanathan et al., 2018). To develop RapID, scientists engineered a mutant BirA* from B. subtilis...

...many distinct CRISPR therapies to match these different mutations. For each therapy, effectiveness and ...’s size makes it difficult to deliver via gene therapy, so researchers have set their sights on another...work with dystrophin exon skipping? For such a therapy to be successful, multiple requirements must be...minimal risk of off-target editing. In order for the therapy to persist over time, stem cell editing would be...in DMD, and it’s estimated that exon skipping therapies would be applicable to 80% of DMD patients. ...characterizes additional, unique facets of CRISPR DMD therapy. Long et al. show that AAV-CRISPR does not cause...represent a great step forward for CRISPR gene therapy, it’s important to realize that DMD is a simpler...

...discovery/development of genetic targets and RNA therapies. [6]. In 2014, we wrote a blog post on CRISPR’...researchers are now using it to investigate potential therapeutics for complex genetic diseases. So we’re revisiting... may not be the most likely candidate for gene therapy, however, because the disease affects multiple ...suggest it might work well enough to consider as a therapeutic.” There are many fundamental questions about ...mutations when using this technology. When CRISPR therapies are being given in vivo, it is difficult to use... References 1. Zhang B. 2021. “CRISPR/Cas gene therapy.” Journal of cellular physiology.  https://doi....1389201021666200621161610 4. Uddin et al. 2020.  “CRISPR Gene Therapy: Applications, Limitations, and Implications for...

...Various AAV Serotypes and Their Applications in Gene Therapy: An Overview. Cells, 12(5), 1–41. https://doi.org... Gene Delivery to the Hypothalamus. Human Gene Therapy, 21(6), 673–682. https://doi.org/10.1089/hum.2009.169...Carroll, S. J. (2019). Astrocyte-selective AAV gene therapy through the endogenous GFAP promoter results in... in the rat spinal cord following injury. Gene Therapy, 26(5), Article 5. https://doi.org/10.1038/s41434...Transduction and Volumetric Spread in the CNS. Molecular Therapy - Nucleic Acids, 8, 184–197. https://doi.org/10.1016...corticospinal tract: Comparison of four promoters. Gene Therapy, 28(1–2), 56–74. https://doi.org/10.1038/s41434...

...human gene therapy. Molecular Therapy: The Journal of the American Society of Gene Therapy, 14(3), 316...AAV1 was the first viral vector approved for gene therapy (Issa et al., 2023). It may be a good choice for...high transduction efficiency in multiple tissues, rapid transgene expression, and can cross blood vessel...rAAV treatment, which can pose problems for gene therapy applications. Cross-reactivity of AAV serotypes...AAV7, and AAV8 in the Mouse and Rat. Human Gene Therapy, 19(12), 1359–1368. https://doi.org/10.1089/hum...Various AAV Serotypes and Their Applications in Gene Therapy: An Overview. Cells, 12(5), 785. https://doi.org...in Mouse and Human Skeletal Muscle. Human Gene Therapy, 31(3–4), 233–240. https://doi.org/10.1089/hum.2019.173...

... research focuses on protein innovations for therapeutic and vaccine design. We all want tools that expand...bearing an alkyne; the two components lock together rapidly and selectively in all sorts of contexts. But the...from the regular building-blocks of living cells, rapidly forming covalent bonds to specific partners under...Adding function to My Favorite Protein (MFP) through rapid covalent coupling. Created with BioRender.com. ...intein SpyTag003/SpyCatcher003 SpyTag/SpyCatcher Rapid covalent bond formation between a genetically encoded...SpyCatcher) SnoopTag/SnoopCatcher SpyTag/SpyCatcher Rapid covalent bond formation between a genetically encoded...expansion DogTag/DogCatcher SpyTag/SpyCatcher Rapid covalent bond formation between a genetically encoded...

...development of toolkits such as the SapTrap CRISPR/Cas Toolkit and SapTrap-SEC kit for C. elegans genome engineering...unprecedented ease. These new C. elegans approaches rapidly enrich for editing events without the need for ...rescued progeny, which makes screening extremely rapid. This stringent selection allowed me to optimize...built on this work, discovering that melting and rapidly cooling dsDNA repair templates dramatically boosted...repair template of your choosing. Tools such as SapTrap Builder greatly simplifies design of these constructs...editing in other organisms and systems, allowing rapid progress in a wide range of fields.   Thank you...genetics.114.170423 Schwartz ML, Jorgensen EM (2018) SapTrap vectors for introducing point mutations with unc...

... interest.  SapTrap Tool Kit for CRISPR Cas9 edits At Addgene, we have the entire SapTrap toolkit from...from the Jorgensen lab. SapTrap is a reagent toolkit and plasmid assembly line for the creation of CRISPR...choosing (Schwartz and Jorgensen, 2016). Using SapTrap, this CRISPR plasmid can be created in a single...single tube. You can also find additional SapTrap plasmids used for introducing point mutations that was published... Schwartz, Matthew L., and Erik M. Jorgensen. "SapTrap, a toolkit for high-throughput CRISPR/Cas9 gene...PMC4905529.  Schwartz, M. L., & Jorgensen, E. M. "SapTrap vectors for introducing point mutations with unc...resources on the Addgene blog Learn more about SapTrap Read about CRISPR/Cas9 in C. elegans Browse our...

...of combining targeted molecular therapies for cancer with chemotherapies, or developing innovative mobile...clinical research to discover and test the best new therapeutics. Consultants often work with such teams to help...human resources towards the development of a new therapy based on its market need and anticipated commercial...context of significant clinical uncertainties and a rapidly evolving healthcare space. The consultants who ...at the core of the suite of benefits a given therapeutic approach brings to patients, doctors, and insurance...and the commercial applications of a particular therapy. With your scientific knowledge behind you, you...business and scientific understanding of successful therapeutics can be a great fit for former healthcare consultants...