...timely feedback. We often associate feedback with end-of-the-year performance review, but feedback should...assume that reports want to succeed at work and providing feedback is needed for that. In many cases reports... for the feedback, but at least you can start making changes for the best. Positive feedback is equally...you team feel safe. Your team members need to trust you and you need to encourage them to speak their minds... and by offering advice on areas where you might need some help. Today in our transferable skills guide...! I was a poor delegator at first. I knew what needed to be done and how to prioritize but I  just added... is no way around the fact that new team members need to be trained, which may seem like a waste of your...

...that can’t set seeds. I study the development of the mutant plants, but the seeds needed for experiments...the green seedlings that don’t have the transgene and save the red seedlings to collect seed for further...RUBY-expressing Arabidopsis seed following floral dip transformation next to an untransformed seed from the same plant...genes usually involves killing the untransformed seedlings or callus (undifferentiated plant cell culture...dip). In the next generation the CRISPR construct needs to be segregated away to prevent continued production...simplify the identification of these mutants will speed up my work and make it easier to study these mutants... plants will be red and will be able to produce seeds. However, they will not be useful to study because...

...materials, you’ll need to use aerosol-tight caps, compatible with your centrifugation speed, to cover the ...plasmid (packaging plasmid). Viral production then proceeds by transfection of all three plasmids into a producer...proto-oncogenes, which can lead to cancer. These viruses need to be handled in a BSL-2 (or BSL-2+) lab and require... your lab coats and double gloving, so that, if needed, a layer can quickly be removed and disposed of...materials, such as lentivirus. Most of this work will need to be done in a biosafety cabinet. Many researchers...to be done outside of a biosafety cabinet, you’ll need to take extra steps to ensure that particles are.... Finally, when spinning down lentivirus, you’ll need to use centrifuge bin covers, which you should open...

...of interest. You can also just follow feeds curated by Meta. Feeds automatically update as papers are published...personalized feed is simple. You can enter as many search terms as you like, and your feed will update ...your feed which means only papers than contain all of your search terms will show up in your feed. Feeds...score, is also displayed in your feed. By clicking on a paper in your feed you can see suggested related ...characters! Using Twitter to find papers  You’ll need to start following relevant accounts in order to...search engine. Instead, you create your own research feed by entering keywords, concepts, journals, and/or... to help you expand your search. Results in your feed are ranked based on their influence on the network...

...exactly your lab needs from an ELN (the full article is available here):   Does your lab need to keep paper...exported or printed from the ELN when needed. Whether it is because you need to keep paper records as well as...to do it when needed. How many people from your lab would use the ELN i.e. do you need a system that ...research? Electronic lab notebooks and researcher's needs The familiar paper-based lab notebook is still a...institutions tend to think about the long term, so they need a solution for which they are sure that there will...electronic lab notebook starts with defining your lab’s needs The values of ELNs show in the long run as they ...currently exchanging information with your team? If you need to exchange information regarding lab work with ...

... if needed. And since we live in the digital age, make sure to research any software you may need: cost...possible COVID-driven virtual drain. Audience needs high speed internet access. High probability of real-... of any given country have equal access to high-speed internet. Before you begin any work, make sure you...produce an outcome you’re happy with. Know if you’ll need specialized equipment and/or expertise from the ...to tell a compelling story in an image or do you need a full exhibit? In a five minute activity at a street...demanding format. Equipment, expense and expertise needed can increase exponentially with complexity. ... and physically. Easy to incorporate visuals if needed. Traditional media formats are quite competitive...

...just as easily discover you need to add some people if you find there’s a need for an expertise your current... and by offering advice on areas where you might need some help. Today: Teamwork. My first experience ...may be relatively permanent; some teams may only need to exist for a year, or a few months, or even a ...project that never ends is a poorly planned project indeed. Mission team A Mission Team is assembled to make...requires a broad knowledge base is going to necessarily need a diverse group of people to provide that base. ... out the door much faster, but getting helpful feedback from other people who have a stake in the outcome... Social interaction is a good thing Part of you needs to interact with other people, whether you want ...

...additional data needed to rule out alternative models or to strengthen your new model?  PFs need to be regularly...your postdoctoral experience, define what will be needed to achieve your intended next career stage and ...competitive for a Group Leader position, what will you need? Multiple first or corresponding author publications... you get there and more practically, when do you need to be there? A typical biomedical postdoc fellowship...Group Leader position, key first author manuscripts need to be ready to write by Year 3.5. Thus, it is critical... directions of the lab, gives you constructive feedback on your research, and teaches you how to write...mentor is. If the person makes time for you, as needed, this is a strong indicator that this person cares...

...that we chose needed to fulfill certain requirements: Customizable to our specific needs Modern and well-supported...usage of the scripts needs to be customized in the pipeline for the specific needs of the user. Thus, a... repository continues to expand, we realized the need to modernize our approach to handle larger volumes...              DNA sequencing drives our bioinformatics needs       Fig. 1: QC'ing data at Addgene is like...been crucial for us to keep pace with our current need to analyze nearly 300 sequences each week. Our internal... challenge: automating the bioinformatics steps needed to transform the raw sequencing data into a complete...Seqera’s mission and our own, as both address the need to accelerate science using different approaches...

...spent on details that do not yet need to be decided. Figure out what you need to accomplish to get to the ... meeting at all. When no discussion is needed and you just need to give everyone a heads up (for example...everyone’s time by considering the level of meeting you need. Project Meetings: You do not have to include every...workload, updates on current projects, and offer feedback. The employee should ask for input on projects...the manager know if any additional resources are needed. All-Company Meetings: As your organization grows...whole thing together. How long do you actually need? Just like liquids, meetings tend to fill the shape...of their container. Consider whether you actually need a full hour to achieve the purpose of the meeting...

...single most important skill that scientists need to succeed in their work. While it's not always recognized...scientist needs to be able to communicate with their colleagues and peers. Scientists also need to be able...substitute for feedback from others.  Seek out members of your target audience and ask for their feedback before...will have in the world is the value that you can succeed in communicating. Even the most rigorous, insightful... interactions, ask yourself how every piece of feedback you receive generalizes.  If someone suggests ...   To address that 'why' question effectively, you need to make your audience the focus of every sentence... write and step you take.  Why does the audience need to know this? How will they react to it - what will...

...testing new products or methods, using metrics and feedback, taking a look at what works and what doesn’t,...can guide viewers through. I know cameras, but we needed someone else. Design: Jennifer thankfully volunteered.... It’s wonderful and simple to use, but when you need to juggle filming and performing on camera, it’s...No scripting made this a lot easier and I didn’t need to be there directing. Everyone’s schedules were...While this works well for social media videos, we need as much detail as possible when showing lab methods...  Design: The previous cycle made me realize we needed to focus on ways to shoot safely at a distance ...out of reach for Addgene.  So, onto research! I needed to design a system that would allow me to set up...

...that need to be resolved. If there are any forms that need to be signed, or manuscripts that need to be...their ideas confidently while also considering the needs of other people. Some students and postdoctoral .... It is in your supervisor’s interest that you succeed, and if you cannot resolve a problem on your own...reach out for help so you can get the support you need. 2) Prepare an agenda for every meeting Supervisors...brainstorm about solutions that will meet both of your needs. 6) Put important agreements in writing One of ...meeting with an email that summarizes what you have agreed upon and your action items. This will give your... what you have discussed and add suggestions if needed. 7) Always follow through on your end of the deal...

...Horticulutre Biotechnology, he did his PhD in Plant Breeding, Genetics, and Biotechnology at Michigan State...award from nonprofit and Addgene collaborator, Seeding Labs, for lab equipment that will help accelerate...about Dr. Kwapata’s current work and thoughts on the need for more scientific collaboration across the globe...:41 : Kingdom on the difficulties surrouding and need for biotech research in Malawi 11:41-14:53 : Kingdom's...challenges in Malawi, and more 23:45-28:07 : How Seeding Labs is helping advance Kingdom's research 28:08... Kwapata for taking the time to talk to us and Seeding Labs for connecting us with him. Learn more about...about our recent collaboration with Seeding Labs here. Special thanks to our sound engineer/producer, Eddy...

...cytometry is commonly referred to as bleeding. When two fluorophores bleed into each other, it means that the...emission spectra to avoid bleeding as much as possible. However, when you need to stain for multiple markers...different target proteins in your flow analysis, you'll need to use fluorophores that attach to the target. When... When you design a flow experiment, you'll need to pick a fluorophore for each target and ensure the fluorophores...properly stain many markers with any fluorophore. Bleeding Before we start building your first panel, we'... a protein and FITC an organic molecule), their bleed is so strong that these spectra almost overlap. ...markers, some bleeding is often unavoidable. But don't worry, there are strategies to resolve this issue...

...competitive CV You don't necessarily need a Nature/Cell/Science paper. You do need multiple first/corresponding...solicited for feedback), possibly the Dean, and even some people in your field that still need to understand...members will NOT be in your area. Therefore, documents need to minimize jargon. Equally importantly, you really...me that their search committee is different and needs to be told all of the nuances of the research in...Bottom line is to appreciate that your audience needs to understand your proposal to give you proper consideration...your research because you waded too deep into the weeds. Check out Joanne Kamens's "10 Steps to a Perfect...idea in a swamp of unnecessary details. You DO NOT need to present a detailed review of your field or the...

...Luna, Joan Reede, Alberto Roca, and Jessica Tytell made it was obvious that more data is needed for us to...outcomes. Some of the more specific contours of what we need to know in order to even assess the current state...the professional skills and research independence needed to pursue his or her chosen career path,” but individual...companies and organizations (including Addgene), that need people who are data oriented, scientifically minded...graduate students and postdocs. Trainees however, need to be made more aware of the fact that these positions...highlights just this point. As part of this, trainees need to be able to network and interact more with people...well as young PIs and all gave advice on how to succeed in publishing in the current academic environment...

...hear you saying “We know this already!” But, you do need to be able to research and write your application...fresh eyes and correct it (and, believe me, you will need to correct it). It’s easy for reviewers to spot ...department) to read over your application and give you feedback. They’ve most likely been through the process ... your area are looking for. Remember though, you need to leave them time to read it, give it back to you...Funding agencies want value for their money so you need to make it clear that you will be doing your research...you have been working for the last few years, you need to be very clear that you are staying because it...the world for your research to rapidly progress. Need Help Building Your Scientific Network? Check out...

...-international focus. Candidates in need of an industry H-1B need to be aware of the April 1 deadline ...necessary and no job offer is needed for this category. To qualify, you need to satisfy 3 out of the 10 ... their work in the US have the added burden of needing sponsorship. If you are in this position, you should... green cards that are well-tailored to meet the needs of researchers. The categories are as follows: ...organizations can be a good choice for individuals needing sponsorship. Academic H1B The academic H-1B allows...your case – the invitation to be a peer reviewer needs to be addressed to your own personal email directly... not allow for individuals to self-petition. You need the support of your institute to do this one. Universities...

...list. Need feedback on their work but prefer to be approached as peers rather than subordinates Need mental... is hard and most people have some learning they need to do before they can be good at it. Like any other...work harder and contribute more. Assuming basic needs are being met (i.e. enough pay and benefits to live...to work mostly at home. Having a high degree of freedom and diversity built into their jobs Chance to do... to identify the work style of each team member) Need challenging work, opportunities to pursue and problems...people, not just scientists) Are self-directed, but need precise leadership and support from their superiors...comfortable with imposed deadlines (there’s that need for flexibility, sometimes taken to an extreme by...