...sharing. Listen to learn more about Darrell, his research, and his focus on open science. Listen to the interview...Darrell strives to promote open source biology in his own lab, at the Center for Regenerative Medicine,...Center for Regenerative Medicine. Darrell describes his career goals and what drives him as a praticing physician...

...  Erik Snapp just released version 3.4 of his eBook, How to Get a Faculty Position. This edition has ...Snapp, who is always looking for ways to improve his eBook, adds: "If you have any questions, comments...email me at [email protected]." You can find his original blog post below! --Ed.  Eight years ago...formula to guarantee a reader the faculty job of his/her dreams. There are too many moving parts in the...luck with your search!   Erik Lee Snapp received his PhD in Molecular Microbiology and Immunology from...from Oregon Health Sciences University, did his postdoctoral fellowship with Jennifer Lippincott-Schwartz...Research Campus of the Howard Hughes Medical Institute. His interests include the quality control of secretory...

...further with his new company, Science with Tom. In this podcast, we learn more about Tom and pick his brain ...and communicate science. Learn more about Tom on his youtube channel and at sciencewithtom.com.   Special...Rosalind Franklin vs. Watson & Crick - Science History Rap Battle A great example of science story telling...and their importance in understanding biological history. 4. Please Don't Kill My Hive Beautifully filmed...

...affinity tag is the polyhistidine (His) tag. Small and unlikely to affect function, His-tagged proteins can...using a Ni2+ column. Like other affinity tags, a His tag can be fused to either the N- or C-terminus of...for purification as elution requires low pH poly His HHHHHH  0.8 Affinity and Purification Very small ...which they can be added to a purification scheme, His tags are frequently combined with other tags for ...Vectors from the Berkeley QB3 MacroLab Eric thanks his wife, Annette Sievers, Ph.D., for her tips and guidance... signaling peptide (Bio), flanked by hexahistidine motifs (6xHis) MBP Large Protein  40 Solubility and...the tag size quickly – modifying the length a polyhistidine tract does not greatly alter the size of the...

...number of institutions in Ghana and most recently got his PhD in cell and molecular biology at the University...Novartis. Listen to learn how Kwabena plans to use his research experiences as well as plasmids from Addgene...50 : Why Kwabena chose to go back to Ghana after his PhD and the need to de-mystify molecular biology....

... work in DNA repair informed his decision to work with CRISPR during his postdoc 12:01 - 13:21 : The Wyss...Work with Cas9 Activators Michael Koeris Explains His Path from Grad Student to Entrepeneur Find Resources...

...As MIT's Ed Boyden explains it, his goal is "to understand the brain at a level of abstraction that enables... fix it when things go awry. To reach that goal, his group develops tools to tinker with brain circuits... article published in Nature Methods, Boyden and his team describe the discovery of a light-sensitive ...optogenetics advance, the big questions that motivate his interest in neuroengineering, and some of the optogenetic...who may not be experts, can you give me a brief history of optogenetics? How long has this idea been around...

...optimized CyPet pCyPet-His Cyan Bacterial Expresses CyPet with C-terminal His tag SCFP3A Cyan Mammalian...optimized YPet pYPet-His Yellow Bacterial Expresses YPet with C-terminal His tag SYFP2 Yellow Mammalian...Cyan Bacterial Expresses Aquamarine with N-terminal His tag pAquaN1 Cyan Mammalian Expresses mammalian ...interactions in living cells using a microscope. Historically, molecular interactions were detected by indirect...

...which can be carried out using Addgene plasmids. His-tag labeling reagents You may already have a FRET...used for protein purification: the NTA binds to the His tag on your protein and the coupled Cy fluorophore... Tc: CCPGCC 50 40-72 Intact Cells Cy3NTAc Poly His  44 35-63 Plasma membrane protein, permeabilized ... Tc: CCPGCC 48 38-70 Intact Cells Cy3NTAc Poly His  63 50-90 Plasma membrane protein, permeabilized ... Tc: CCPGCC 54 42-78 Intact Cells Cy3NTAc Poly His  65 52-94 Plasma membrane protein, permeabilized ...efficiencies between 80% and 10%. C Cy3NTA and Cy5NTA bind His tags similarly but with differing R0 values, thereby...department at Brigham and Women’s Hospital in Boston, MA. His interests include developing novel fluorescent tools...

...cooks and cleans with his family, “sometimes listening to audiobooks.” Church and his family spend family...Ellis highlights the importance of acknowledging his teams successes. “When PhD students graduate there...

...instead started a company around his own research. He was clear on his science, and found a solution. However...manageable laboratory environments. His business skills enhance his passion for science. Follow him on ...to provide the resources required for him to meet his goals. Therefore, he turned from academia altogether...

... Children’s Hospital and Harvard Medical School. His lab studies the molecular genetics of blood cell ...cell development and disease. Learn more at his webpage, bauerlab.org.     Matthew Canver is an MD/PhD student...laboratories of Stuart Orkin and Daniel Bauer. He received his BSE in Bioengineering from the University of Pennsylvania...these may require restriction fragment length polymorphism analysis, allele-specific PCR, T7EN1 cleavage...

...problems resulting from past practices and mores.  His list is dire:  The current system is unsustainable... be in academia to make change.  He talked about his own unique way of funding basic research at NEB and... of the Novartis industry postdoctoral program.  His strongest comments were about the astounding lack...

...Genome Institute of Singapore who recently started his own lab. We discuss some of the joys and difficulties...Investigator at the Genome Institute of Singapore. His lab focuses on producing biotechnologies in the areas...

... Embracing Serendipity Nicolas Mathis, who shared information on his open software that predicts efficiencies.... Illuminating Choices Paul Heisig, who shared his flow cytometry expertise in three posts. An Introduction...

...given over the course of the day. His talk, which focused on the history of genome engineering from ZFNs...mentioned specifically in Dr. Zhang's introduction. His willingness to share reagents so freely with the ...analog versions of each, and Tim Lu even compared his "memory" gene codes to a Turing machine (also discussed... of the book on my way to the meeting, and its history of code making and code breaking is wonderfully...

...about his own biases--many of which will be shared by fellow scientists--but that doesn’t make his introduction...case studies are “old,” they lead Zimmer to one of his most critical observations: “Today the most successful...if Zimmer was able to rewrite the last chapter of his book today--the day you are reading this review--...

... successful applicants and get chosen to work in his or her lab of choice? Is the group micromanaged or...to the lab to see how they fit in with the team. His advice is “if you’re within a year from the end of...the lab for a full day, gives a 1 hour talk about his or her interests, and spends one-on-one time with... hires are simpler because of their documented history in science. Potential graduate students typically...

...genes and proteins associated with pathogenesis. In his future work. Dr. Bengyella would like to use the ...have a heavy focus on teaching. He hopes to teach his molecular biology students about the functions of...

...breakdown Intro: How Tom ended up in academia and his research goals. From grad school to a startup and...the UK lead of the yeast synthetic genome project. His team also investigates bacteria and extremophiles...