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Addgene
Showing: 191 - 200 of 602 results
  1. Why Do I Need an MTA Anyway?

    Type
    Blog Post
    Published
    May 1, 2014, 8:23 p.m.
    ...transfers. When Addgene entered the plasmid sharing space, we heard over and over again that the legal documents...experiments with confidence. Benefits of the UBMTA: Peace of mind Addgene has adopted the terms of the Uniform... Recipients: Streamlined Processing: When you place an order, Addgene generates the MTA and emails it...
  2. Same Addgene, New Look - Why We Redesigned Our Homepage & Mascot

    Type
    Blog Post
    Published
    Dec. 5, 2019, 1:49 p.m.
    ...relatively the same for quite a while and hadn’t kept pace with our growth over the last few years. Some of... professional, and modern. Instead of trying to place all of the content above the fold, we decluttered...elements and allowed our content to dictate the space. We wanted to comfortably tell our story, to get...
  3. CRISPR 101: Cas9 Nickase Design and Homology Directed Repair

    Type
    Blog Post
    Published
    March 15, 2018, 12:59 p.m.
    ... a double-strand break, and when used with two adjacent gRNAs, can lower the probability of off-target... of the targeted region, whereas PAM-in designs place the PAMs closer together in the middle of the targeted...sites separated by 51-68 bp Use D10A for HDR Place intended insert in between nick sites Use 40 nt...
  4. Tips for Improving Your Next Manuscript

    Type
    Blog Post
    Published
    Nov. 22, 2016, 3:30 p.m.
    ... Many thanks to our guest blogger Sean Stacey! Sean D. Stacey is a Ph.D. student at East Tennessee State...This post was contributed by guest blogger Sean Stacey who recently attended both online and on site courses...
  5. Early Career Researcher Toolbox: Free Online Molecular Biology Tools

    Type
    Blog Post
    Published
    Sept. 12, 2023, 1:15 p.m.
    ... free online molecular biology tools all in one place. Plasmid mapping These tools are for viewing, editing... looking at plasmid maps and viewing sequencing traces, while the paid version provides more tools for...Removes non-DNA characters, such as numbers and blank spaces, from the text of a DNA sequence, making it ready...
  6. Bringing Sustainable Practices to the Lab: Recycling

    Type
    Blog Post
    Published
    Feb. 14, 2019, 2:35 p.m.
    ...your workplace and you could end up making a huge impact on how waste is handled. A lot of places have...on waste, but also on culture. Encourage your workplace to become a leader in sustainability. Sign up...
  7. Embracing Serendipity: A Crucial Element in the PhD Journey

    Type
    Blog Post
    Published
    April 25, 2024, 1:15 p.m.
    ...perspectives. Such activities foster a mindset that embraces the unexpected and encourages exploration of tangents...ideas and approaches. Moreover, the ability to embrace serendipity fosters adaptability, a crucial skill...your academic journey, remember to leave a little space for the unexpected — it might just be the key to...
  8. Running for Rare Disease, Running for FOP, Running for AJ

    Type
    Blog Post
    Published
    Sept. 15, 2016, 2:30 p.m.
    ...protein engineer currently working at Sanofi Pharmaceuticals in Cambridge, MA. When I joined Sanofi/Genzyme...continue to help bring HOPE to AJ.   PS: The superhero race is the perfect event for supporting AJ, who is a...protein engineer currently working at Sanofi Pharmaceuticals in Cambridge, MA. His work experience is in...
  9. The Stingy Scientist: How the Baby Gel Box Was Born

    Type
    Blog Post
    Published
    April 15, 2014, 3:11 p.m.
    ... amazing job shuttling unwanted lab supplies to places that really need them.  When I was in graduate ...indefinitely at room temperature (thus also saving freezer space).  The procedure becomes less attractive when noting...very thin gel on a large glass slide using only surface tension.  It's a bit tricky to pour the right amount...
  10. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    Published
    March 13, 2014, 4:08 p.m.
    ..."CRISPR", short-hand for Clustered Regularly Interspaced Short Palindromic Repeats, in Pubmed returned...want to use double nickase, you could express two spacers and use PX335 to express the Cas9n (nickase). The...usually adds it when they order the oligo. If your spacer sequence starts with a 'G', you naturally have ...
Showing: 191 - 200 of 602 results