We narrowed to 839 results for: LEA

1. Build Your CRISPR Vocabulary

   Type

   Blog Post

   Published

   July 2, 2024, 1:15 p.m.

   ...CRISPR-associated.” The most famous of these proteins is Cas9 nuclease, but there are many other Cas proteins as well...

2. Using Ultrasound to Image Bacteria in vivo: Acoustic Reporter Genes

   Type

   Blog Post

   Published

   June 19, 2018, 1:38 p.m.

   ...29300010. Additional Resources on the Addgene Blog Learn more about in vivo imaging tools The luciferase ...

3. Plasmids 101: Walkthrough of Addgene’s Snapgene-Powered Quality Control Process

   Type

   Blog Post

   Published

   Feb. 1, 2018, 3:07 p.m.

   ...sequence with one or more enzymes.   Click here to learn how to run an agarose gel     We’ve worked with...

4. An Addgene Co-op: The Intersection of Fun, Friendship, and Work

   Type

   Blog Post

   Published

   June 29, 2023, 7 p.m.

   ...nervous (I mean I WAS presenting to three department leads, I probably should have been nervous), but I wasn...

5. CRISPR 101: Drug discovery and CRISPR

   Type

   Blog Post

   Published

   May 4, 2023, 1 p.m.

   ...variants of unknown significance – mutations with unclear effects on disease pathology - to determine if ...

6. The Open Repository of CRISPR Screens: CRISPR Screen Data in One Place

   Type

   Blog Post

   Published

   Jan. 21, 2020, 2:30 p.m.

   ...Resources on Addgene.org Read our CRISPR guide Learn more about plasmid pooled libraries Find CRISPR ...

7. 10 Ways to Share Your Science!

   Type

   Blog Post

   Published

   May 13, 2014, 8:34 p.m.

   ...communicating your research by guest blogging here, just leave a comment if you're interested and our team will...

8. Plasmids 101: Origin of Replication

   Type

   Blog Post

   Published

   Nov. 11, 2020, 2:23 p.m.

   ... any questions? Need something clarified? If so please, post in the comments.  References Chatterjee A...

9. Safe Port in a Storm...How Addgene is Weathering the Pandemic

   Type

   Blog Post

   Published

   Sept. 17, 2020, 1:15 p.m.

   ...for scientists that helps them share reagents — leading to open, reproducible science,” said CZI Head of...

10. Viral Genome Sequencing - A One Stop Shop for AAV Identity and Serotype Confirmation

    Type

    Blog Post

    Published

    April 23, 2020, 1:15 p.m.

    ... Resources on Addgene.org Find AAVs at Addgene Learn more about Addgene's viral service Find protocols...

11. Summer SciComm: Science Storytelling

    Type

    Blog Post

    Published

    Sept. 16, 2022, 1:15 p.m.

    ... science communication and can use what you've learned to share your science with others.    My favorite...

12. HA Frankenbody, a New Imaging Tool to Visualize Single Molecules and Nascent Peptides

    Type

    Blog Post

    Published

    Oct. 10, 2019, 12:41 p.m.

    ...PMC6610143. Additional resources on the Addgene blog Learn about visualizing protein turnover Download the ...

13. The Golden Stopwatch Award: Recognizing Facilitators of Research

    Type

    Blog Post

    Published

    Sept. 5, 2019, 1:06 p.m.

    ... research materials as quickly as possible. (To learn more about why MTAs matter, check out another blog...

14. Multifunctional Neural Fibers: Modulation from Gut to Brain

    Type

    Blog Post

    Published

    Aug. 31, 2023, 1 p.m.

    ...reliable optically evoked neuron activity for at least several months after implantation of fibers, indicating...

15. Antibodies 101: Epitope Tags

    Type

    Blog Post

    Published

    May 11, 2023, 1:15 p.m.

    ...suitable for use in apoptotic cells as Caspase3/7 both cleave after the DVPD sequence. Check out Addgene’s monoclonal...

16. Antibodies 101: Chimeric Antibodies

    Type

    Blog Post

    Published

    April 25, 2023, 1:15 p.m.

    ...antibodies may be the right solution for you. Read on to learn more. What is an isotype? Antibodies are composed...

17. Science Careers: Unruly Interests Feed Many Paths

    Type

    Blog Post

    Published

    Sept. 26, 2017, 12:12 p.m.

    ...I knew anything about being an editor - that I learned on the job. The day-to-day work of a science editor...

18. Viral Vectors 101: Transductions

    Type

    Blog Post

    Published

    April 30, 2024, 1:15 p.m.

    ...genetic cargo — the viral genome — is swapped, at least partially, for a nucleic acid cargo of your choice...

19. Rewiring Metabolic Circuitry with CRISPR RNA Scaffolds [Video]

    Type

    Blog Post

    Published

    April 7, 2015, 4:21 p.m.

    ...catalytically dead Cas9 protein (dCas9) that lacks endonuclease activity coupled with a customizable single ...

20. Reagent Repositories Are Speeding up Science During the Pandemic

    Type

    Blog Post

    Published

    Aug. 4, 2020, 1:15 p.m.

    ...Read more about COVID-19 databases and resources Learn about Addgene's work to share plasmids to all scientists...

21. Quantifying DNA? Here are Five DNA Quantification Methods to Consider

    Type

    Blog Post

    Published

    July 21, 2020, 1:15 p.m.

    ...the best way to elute and store your plasmid DNA Learn about the different grades of plasmid DNA preps ...

22. Crowdfight, a Platform to Boost Scientific Collaboration During COVID-19 and Beyond

    Type

    Blog Post

    Published

    June 1, 2021, 1:15 p.m.

    ... the preprint repository BioRxiv. This example clearly illustrates the power of collaboration, which despite...

23. FPbase: A new community-editable fluorescent protein database

    Type

    Blog Post

    Published

    May 16, 2018, 1 p.m.

    ...better way to store and represent within-study photobleaching comparisons.  We very much appreciate feedback...

24. The Power Behind NGS Plasmid Validation: seqWell

    Type

    Blog Post

    Published

    April 19, 2017, 3:25 p.m.

    ...collection.   Check out the SeqWell website to learn more about our plexWell kits and services! Many ...

25. Resume Writing for Non-academic Science Careers

    Type

    Blog Post

    Published

    Dec. 18, 2014, 4:06 p.m.

    ...what you are capable of. You would be surprised to learn how often I see resumes from good candidates that...

26. Choosing Your Fluorescent Proteins for Multi-Color Imaging

    Type

    Blog Post

    Published

    Oct. 9, 2014, 3 p.m.

    ...most detectable and so should be used to tag your least abundant proteins, with the blue and infrared channels...

27. Building and Validating Recombinant Antibodies: A Case Study

    Type

    Blog Post

    Published

    April 5, 2022, 1:20 p.m.

    ...techniques and troubleshooting methodologies drew me to learn more about the process that was used to create this...

28. Antibodies 101: Flow Cytometry Controls

    Type

    Blog Post

    Published

    June 6, 2024, 1:50 p.m.

    ...should have a FMO sample for any marker without a clear positive and negative population. If you’re unsure...

29. Viral Vectors 101: Viral Vector Elements

    Type

    Blog Post

    Published

    July 25, 2023, 1 p.m.

    ...protein that helps with virion assembly and particle release from late endosomes when AAV is trafficked. Rep...

30. Plasmids 101: Screens vs. Selections

    Type

    Blog Post

    Published

    Nov. 10, 2022, 2:15 p.m.

    ...both a screen and a selection, it is important to clearly define what type of factor you are looking for ...

31. REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

    Type

    Blog Post

    Published

    Feb. 10, 2016, 3:30 p.m.

    ...Cloning Techniques like SLIC and Golden Gate Cloning Learn How to Verify Your Plasmid Read our Plasmids 101...

32. Antibodies 101: Polyclonal Antibodies

    Type

    Blog Post

    Published

    July 14, 2021, 1:15 p.m.

    ...of antibody for research, monoclonal antibodies Learn about the advantages of using recombinant plasmid-based...

33. Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options

    Type

    Blog Post

    Published

    May 11, 2021, 1:15 p.m.

    ...its localization in the membrane. The lesson we learned from these studies is that the tag protein can ...

34. It’s Bioelectric! An Exciting Interdisciplinary Field of Research

    Type

    Blog Post

    Published

    Oct. 29, 2019, 2:52 p.m.

    ...an otherwise normal genomic background, can even lead to the formation of whole eyes from cell types that...

35. Troubleshooting Your Plasmid Cloning Experiment

    Type

    Blog Post

    Published

    Sept. 24, 2019, 12:55 p.m.

    ...for digestion. PCR Design primers that overlap at least 24 bp with the template and if the sequence has ...

36. Addgene: The First Twenty Years

    Type

    Blog Post

    Published

    Dec. 19, 2024, 2:15 p.m.

    ...Award, which recognizes researchers who have at least one plasmid deposited at Addgene that has been distributed...

37. Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number

    Type

    Blog Post

    Published

    Sept. 4, 2018, 12:53 p.m.

    ... IPTG concentrations. Higher IPTG concentration leads to higher RepA expression which results in high ...

38. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

    Type

    Blog Post

    Published

    June 15, 2017, 2:30 p.m.

    ...Your Fluorescent Proteins for Multicolor Imaging Learn about Fluorescent Protein Biosensors Additional...

39. CRISPRainbow and Genome Visualization

    Type

    Blog Post

    Published

    Feb. 28, 2017, 3:30 p.m.

    ...Read More about Fluorescent Protein-Based Tools Learn Some of the Pitfalls When Using Fluorescent Proteins...

40. CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt

    Type

    Blog Post

    Published

    April 22, 2015, 2:06 p.m.

    ...through Addgene, and where all of it is likely to lead us in the future. Building circuits from CRISPRs...