We narrowed to 557 results for: ESP

1. Quick Guide to Working with Drosophila Part 2: Controlling Gene Expression in Flies with Gal4/UAS

   Type

   Blog Post

   Published

   July 21, 2017, 12:48 p.m.

   ...PMID: 28704652. 6. V. Rodríguez, D. Didiano, C. Desplan, Power tools for gene expression and clonal analysis...

2. Unique and persistent IDs for improved reproducibility: Addgene now supports RRIDs and compact identifiers for all plasmids

   Type

   Blog Post

   Published

   Nov. 20, 2018, 8:17 p.m.

   ...you directly to the lenti-Cas9-VQR plasmid page. Despite their differences, both of these PID formats for...

3. Scientists Map the SARS-CoV-2-Human Interaction Network

   Type

   Blog Post

   Published

   April 9, 2020, 1:15 p.m.

   ... these potential human helpers of SARS-CoV-2 (especially the ones that are druggable) is a key step in...

4. Advice for Scientists Starting a Lab

   Type

   Blog Post

   Published

   April 29, 2014, 1:43 p.m.

   ...decide on the best way to structure roles and responsibilities as we grew.” Initially, he spent the majority...

5. Finding and Joining Your Dream Lab

   Type

   Blog Post

   Published

   April 8, 2014, 2:50 p.m.

   ...for a lab Studies show that job satisfaction (especially for scientists and technical people) is linked...

6. Fluorescent Proteins 101: Luciferases

   Type

   Blog Post

   Published

   July 22, 2025, 1:15 p.m.

   ...abdomen to produce yellow-green light (Figure 2). Despite the title of this post, this reaction is not fluorescence...

7. Viral Vectors 101: Gamma-Retroviral Packaging Systems

   Type

   Blog Post

   Published

   Aug. 6, 2024, 1:15 p.m.

   ...The widespread use of γ-retroviruses (gamma-retroviruses) in cancer and stem cell research has prompted...

8. 7 Tips to Secure a STEAM Internship This Summer

   Type

   Blog Post

   Published

   Feb. 2, 2021, 2:15 p.m.

   ...Read the associated prompt carefully, plan your response, and let your inner wordsmith shine through. The...

9. Plasmids 101: Screening Strategies Used in Plasmid Cloning

   Type

   Blog Post

   Published

   Sept. 15, 2020, 1:15 p.m.

   ...sequence just 1 kb of DNA, custom primers are especially helpful in verifying mutations that wouldn't ...

10. New and Upcoming Viral Vectors - May 2020

    Type

    Blog Post

    Published

    May 28, 2020, 1:15 p.m.

    ...together encode the N and C terminal segments respectively of a GFP dependent Cre. The two segments come...

11. Hot Plasmids - May 2022

    Type

    Blog Post

    Published

    May 26, 2022, 1:15 p.m.

    ...fluorescent protein by a specific transcription response element (TRE) or kinase translocation reporters...

12. Building and Validating Recombinant Antibodies: A Case Study

    Type

    Blog Post

    Published

    April 5, 2022, 1:20 p.m.

    ...previously possible. This advancement could be especially exciting for scientists working to visualize ...

13. Antibodies 101: ChIP

    Type

    Blog Post

    Published

    March 10, 2022, 3:25 p.m.

    ...then processing with a homogenizer, but bone or especially fibrous tissues may need extra steps.  Fragmenting...

14. Hot Plasmids - February 2022

    Type

    Blog Post

    Published

    Feb. 22, 2022, 2:15 p.m.

    ...less than half the size of either Cas9 or Cas12a. Despite its small size, CasMINI is comparable in efficacy...

15. Antibodies 101: Buffers, Storage, and Conjugates

    Type

    Blog Post

    Published

    Feb. 1, 2022, 2:15 p.m.

    ... by emitting a specific wavelength of light in response to excitation by a laser, which is then read by...

16. Communicating Your Science With Help From ComSciCon

    Type

    Blog Post

    Published

    July 19, 2018, 1:12 p.m.

    ...graduate students to become ambassadors for their respective fields. To help attendees launch new organizations...

17. Why and how to start studying chromatin (with Addgene's help)

    Type

    Blog Post

    Published

    June 12, 2018, 11:40 a.m.

    ...scientists with me at the Keystone Chromatin meeting, especially Karen Reddy (@theReddyLab), Thoru Pederson, Joanna...

18. Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering

    Type

    Blog Post

    Published

    May 3, 2018, 1:48 p.m.

    ...protein knockdown measured by flow cytometry, respectively. Cas13d CRISPR array processing is robust, with...

19. Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification

    Type

    Blog Post

    Published

    Nov. 19, 2019, 2:08 p.m.

    ...reversibly bind and release a protein of interest in response to light allows for tagless protein purification...

20. Plasmids 101: Inducible Promoters

    Type

    Blog Post

    Published

    Jan. 18, 2018, 2:34 p.m.

    ...normally inactive and cannot bind the tetracycline response elements (TRE) in a promoter. Tetracycline and...