We narrowed to 554 results for: ESP

1. Hot Plasmids - September 2019 - CRISPR, Auxin Inducible Degradation, MerMAID Channelrhodopsins, and Genome Looping

   Type

   Blog Post

   Published

   Sept. 3, 2019, 1:15 p.m.

   ...While other eukaryotes don’t naturally have this response, they do contain the specific ubiquitin ligase...

2. Summer SciComm Series: A PhD in Science Communication

   Type

   Blog Post

   Published

   Aug. 23, 2022, 1:15 p.m.

   ...people with greater levels of science literacy are especially prone to this, possibly because a greater familiarity...

3. Advice for Scientists Starting a Lab

   Type

   Blog Post

   Published

   April 29, 2014, 1:43 p.m.

   ...decide on the best way to structure roles and responsibilities as we grew.” Initially, he spent the majority...

4. Finding and Joining Your Dream Lab

   Type

   Blog Post

   Published

   April 8, 2014, 2:50 p.m.

   ...for a lab Studies show that job satisfaction (especially for scientists and technical people) is linked...

5. Plasmids 101: Screening Strategies Used in Plasmid Cloning

   Type

   Blog Post

   Published

   Sept. 15, 2020, 1:15 p.m.

   ...sequence just 1 kb of DNA, custom primers are especially helpful in verifying mutations that wouldn't ...

6. New and Upcoming Viral Vectors - May 2020

   Type

   Blog Post

   Published

   May 28, 2020, 1:15 p.m.

   ...together encode the N and C terminal segments respectively of a GFP dependent Cre. The two segments come...

7. The Many Reasons for Irreproducible Research- and a Vaccine to Eradicate It

   Type

   Blog Post

   Published

   Jan. 30, 2020, 2:15 p.m.

   ...they actually are. Throwing out plates that don’t respond as expected, use of representative images, measuring...

8. Why and how to start studying chromatin (with Addgene's help)

   Type

   Blog Post

   Published

   June 12, 2018, 11:40 a.m.

   ...scientists with me at the Keystone Chromatin meeting, especially Karen Reddy (@theReddyLab), Thoru Pederson, Joanna...

9. Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering

   Type

   Blog Post

   Published

   May 3, 2018, 1:48 p.m.

   ...protein knockdown measured by flow cytometry, respectively. Cas13d CRISPR array processing is robust, with...

10. Plasmids 101: Inducible Promoters

    Type

    Blog Post

    Published

    Jan. 18, 2018, 2:34 p.m.

    ...normally inactive and cannot bind the tetracycline response elements (TRE) in a promoter. Tetracycline and...

11. Viral Vectors 101: Viruses as Biological Tools

    Type

    Blog Post

    Published

    March 21, 2023, 1:15 p.m.

    ...which means they do not stimulate a strong immune response when introduced into an organism. This feature...

12. Sharing is Caring: But How to Distribute Open Hardware?

    Type

    Blog Post

    Published

    Feb. 28, 2023, 2:15 p.m.

    ...are still many open questions to be clarified, especially with regard to licensing models. Direct sharing...

13. Fluorescent Proteins 101: Luciferases

    Type

    Blog Post

    Published

    July 22, 2025, 1:15 p.m.

    ...abdomen to produce yellow-green light (Figure 2). Despite the title of this post, this reaction is not fluorescence...

14. A Practical Approach to Choosing the B(right)est Fluorescent Protein

    Type

    Blog Post

    Published

    June 1, 2017, 2:30 p.m.

    ...extinction coefficient (EC) and quantum yield (QY) respectively. The theoretical brightness is calculated by...

15. Addgene’s Viral Service - Why Virus? Why Now?

    Type

    Blog Post

    Published

    March 23, 2017, 2:30 p.m.

    ...services. There was an overwhelmingly positive response to the option of Addgene providing plasmids in...

16. Targeted Mutagenesis with EvolvR

    Type

    Blog Post

    Published

    Feb. 21, 2019, 1:15 p.m.

    ...provide a better understanding of the key residues responsible for this protein-drug interaction.   The Dueber...

17. Q&A with Karen Katz and Carl Paratore, the Newest Members of the Addgene Board of Directors

    Type

    Blog Post

    Published

    Feb. 19, 2019, 1:36 p.m.

    ... he leads the Internal Audit function and is responsible for bringing a systematic and disciplined approach...

18. Hot Plasmids December 2018 - C. elegans RNAi, TurboID, Detecting Viral Outbreaks, and Protein Binding Specificity Tools

    Type

    Blog Post

    Published

    Dec. 6, 2018, 1:34 p.m.

    ...target specific mRNA transcripts for degradation. Despite how easy these experiments are to physically set...

19. Viral Vectors 101: Gamma-Retroviral Packaging Systems

    Type

    Blog Post

    Published

    Aug. 6, 2024, 1:15 p.m.

    ...The widespread use of γ-retroviruses (gamma-retroviruses) in cancer and stem cell research has prompted...

20. Communicating Your Science With Help From ComSciCon

    Type

    Blog Post

    Published

    July 19, 2018, 1:12 p.m.

    ...graduate students to become ambassadors for their respective fields. To help attendees launch new organizations...