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We narrowed to 830 results for: Eras

Showing: 381 - 400 of 830 results

  1. Twenty Years of Sharing: Addgene's Viral Vector Service

    Type
    Blog Post
    Published
    July 30, 2024, 1:15 p.m.
    ...was first developed in 2001, with successive generations developed ever since. GCaMP6 was offered through... added jGCaMP7 and eventually jGCaMP8, later generations of GCaMP6, to the service. While they were both...jGCaMP8 per year from 2016–2023. All three generations are regularly requested and a valuable part of...size of the incremental improvements between generations: jGCaMP8 allows for the “picture” of a train ... former approach and involves reading primary literature, soliciting information from requestors and depositors...Swanson, L. E., & Fan, M. (2016). Practical Considerations for Using Pooled Lentiviral CRISPR Libraries...

  2. How to Write a Scientific Review Article

    Type
    Blog Post
    Published
    Feb. 16, 2017, 3:30 p.m.
    ... to write, to read efficiently, to search the literature, and to synthesize a large volume of information...appreciate the discussion sections of the primary literature as a resource that both summarized results and... sure if I should cite reviews or the primary literature or both. I ended up citing both because I used...the scope and the content. Submit your article! Overall, writing a review can be overwhelming and challenging...Thompson, and Thomas D. Gilmore. "Histone acetyltransferases and histone deacetylases in B-and T-cell ...Addgene Blog Writing Scientific Manuscripts: Literature Searching, Reading, & Organizing Tips for Improving...

  3. Antibodies 101: Reading a Flow Plot

    Type
    Blog Post
    Published
    Feb. 6, 2024, 3:13 p.m.
    ...soon find yourself encountering flow data in the literature. Data reported from flow cytometry experiments...work is done in flow cytometers, and output is generated through measurement of events. An event is something... machine is plotted as a dot on an X,Y axis to generate a dot plot (Figure 1). Clustered events are called...                         Figure 1: While the plot generated often looks like the graph on the left, it might...intersection of X,Y. For flow plots, however, that is generally not true. Because of the variations in fluorescent...Of course, this is quite easy to do on a plot generated in Biorender! While a helpful visualization tool...

  4. The Challenges of Cell Culture

    Type
    Blog Post
    Published
    May 5, 2016, 2:30 p.m.
    ...genotypic and phenotypic artefacts. The life science literature is a litany of false interpretation in cell culture...Down-Regulation of HIF-1α by NDRG2 Expression Attenuates Proliferation and Invasion in Renal Cancer Cells, Marker-independent...out by the commercial repository. Repositories generally ensure cell line identity and purity making it...to the original as possible and so any results generated are reproducible and reliable. When you order ...which can be stored in liquid nitrogen long term, preferably some of these vials will be stored in a separate...identity. The Nature Publishing Group recently reiterated its strict stance on the matter. Funding bodies...

  5. Replacing Paper: Tips for Choosing an Electronic lab Notebook

    Type
    Blog Post
    Published
    July 10, 2018, 1:08 p.m.
    ...that only 11% of the academic research in the literature was reproducible by their groups. Finally results...copes with the growing amounts of digital data generated in labs. While many labs approach the situation...are available on the market today, ranging from general note taking tools, to more scientific field-based...From pictures to excel sheets, researchers today generate vast amounts of digital data during their work...within your lab? In every lab, there are certain hierarchies and restrictions to data access. If these are...walk you through your ELN’s features and this interaction will give you an idea of how responsive and supportive...

  6. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    Published
    Oct. 5, 2021, 1:15 p.m.
    ...member at Albert Einstein College of Medicine. Several sets of near-infrared fluorescent proteins (NIR...the NIR light coming from your source and to your camera.  Two-photon image of miRFP670nano3-expressing...reported widely used dimeric iRFPs (Table 1). In general, the monomeric state of a probe leads to less interference...al., 2018). For detection of protein-protein interactions, we recommend using NIR split reporters based...detect spatiotemporal dynamics of protein-protein interactions. Nat Methods 11, 641-644 (2014). Filonov, G....reporter for in vivo imaging of protein-protein interactions. Cell Chem Biol 20, 1078-1086 (2013). Shcherbakova...

  7. Using Phosphoserine to Study Protein Phosphorylation

    Type
    Blog Post
    Published
    June 23, 2016, 2:30 p.m.
    ...candidate regulatory kinase is known, it’s possible to generate non-phosphorylated recombinant protein and perform...Creating phosphoserine-incorporated proteins The generation of pSer-incorporated proteins requires a molecular...position. Practical tips for your experiments To generate as much pSer-incorporated protein of interest ...be optimized for parameters such as induction temperature and length of induction, which will vary between...between different proteins. Beyond these standard considerations, it is important to determine how much phosphorserine... effects of pSer on enzyme function, protein interaction, etc. by comparison to non-phosphorylated or ...

  8. Plasmids 101: Common Lab E. coli Strains

    Type
    Blog Post
    Published
    Nov. 7, 2014, 2:56 p.m.
    ...suggests there are hundreds. This only includes general lab strains designed for subcloning or protein ...ATPase that is essential for recombination and general DNA repair Reduces plasmid recombination, increases...Δleu mtl1 DH10B Streptomycin MC1061 derivative. General cloning and storage, blue/white screening, leucine...)7697 mcrA Δ(mrr-hsdRMS-mcrBC) λ- DH5alpha   General cloning and storage of common plasmids, blue/white...galK2 xyl-5 mtl-1 rpsL20(SmR) glnV44 λ- JM109   General cloning and plasmid maintenance, blue/white screening...(TetR) TOP10 Streptomycin MC1061 derivative. General cloning and storage, blue/white screening. F- mcrA...

  9. Ghanaian Researchers to Receive Plasmid Awards from Addgene and Seeding Labs

    Type
    Blog Post
    Published
    June 15, 2016, 1:30 p.m.
    ...produce molecular biological tools like Taq polymerase, T7 polymerase, EcoRI, and various fluorescent proteins... elucidating the molecular mechanisms behind interactions between crops like potatoes and their microbial...that enable E. coli and Citrobacter freundii to generate sugars from cellulosic biomass. Dr. Duedu would...construction of new plasmids. Dr. Duedu will also use polymerases produced using plasmids from Addgene to test ...

  10. How Exercise Purges Stress and Keeps Depression at Bay

    Type
    Blog Post
    Published
    Nov. 25, 2014, 4:02 p.m.
    ...unpredictable loud noises and flashing lights - that sent average, control mice into depression. In case you were... skeletal muscle expression of kynurenine aminotransferases, to enhance the conversion of kynurenine into...between the muscle and brain, which may have therapeutic implications, say Hyo Youl Moon and Henriette...psychiatric and cognitive conditions as well as neurodegenerative diseases.” I don’t know about you, but I think...Pettersson AT, Ferreira DM, Krook A, Barres R, Zierath JR, Erhardt S, Lindskog M, Ruas JL. doi: 10.1016...

  11. Science Communication for Everyone

    Type
    Blog Post
    Published
    April 16, 2019, 1:05 p.m.
    ...for general audiences. So far, we are beginning to make an impact on society and science literacy. We ... Addictive Brain and science communication for general audiences. How does The Addictive Brain address...science, especially disease related topics. Neurodegenerative and brain disorders are frequently overshadowed...“manipulate” could mean “illicit tampering” to general audiences while “manipulate” could mean “worked...

  12. Modulate the Activity of 17 Signaling Pathways with One Kit!

    Type
    Blog Post
    Published
    March 22, 2016, 2:30 p.m.
    ...become resistant to drugs, particularly targeted therapies,” said Kris Wood of Duke University Medical Center...that are frequently implicated in cancer cell proliferation, survival, differentiation, and apoptosis. For...Rather, they relied on published studies in the literature to curate the perfect library, obtained the appropriate...researchers, and cloned them into lentiviral vectors to generate a kit which is now available at Addgene. “We called... cancer therapy...

  13. Plasmids 101: What is a plasmid?

    Type
    Blog Post
    Published
    April 2, 2020, 6:17 p.m.
    ...Plasmids 101 will provide you with an overview of general molecular biology knowledge and techniques, and...Site (MCS) Short segment of DNA which contains several restriction sites allowing for the easy insertion...later work.     How do scientists use plasmids? Generally, scientists use plasmids to manipulate gene expression...Green Fluorescent Protein (GFP) Plasmids 101: Luciferase Monitor the level of a chemical in a particular... viruses that can be used in research or for therapeutics Addgene has compiled various educational resources...

  14. Using Ultrasound to Image Bacteria in vivo: Acoustic Reporter Genes

    Type
    Blog Post
    Published
    June 19, 2018, 1:38 p.m.
    ...Administration of Substrate: Some luciferase reporters, such as Renilla luciferase (RLuc) or NanoLuc, require...bacterial reporters that use fluorophores and luciferases to track bacteria in real time, but each of these...Blog Learn more about in vivo imaging tools The luciferase reporter gene The green fluorescent protein reporter...vivo imaging with fluorescent proteins Browse luciferase plasmids Find fluorescent protein biosensors ...

  15. ReFigure: Save Scientific Figures into Dashboards and Share Your Insights

    Type
    Blog Post
    Published
    Dec. 13, 2017, 3:20 p.m.
    ...saves time, knowledge and makes your insights discoverable. Watch this quick video to learn how ReFigure...How much time do you spend reading scientific literature? Very often the data of interest are in 1 or ...preclinical and clinical data about a disease or therapeutic Side-by-side comparison of the same experiment...save analyses with the relevant figures from a literature review. No more hunting through a whole folder...reproducible. For an example, see our ReFigures on the therapeutic use of anti-CD47 antibodies in immuno-oncology...

  16. Hot Plasmids and Viral Preps - July 2021

    Type
    Blog Post
    Published
    July 27, 2021, 1:15 p.m.
    ..., functions at temperatures up to 43 °C and can be used for genome editing in moderate thermophiles.  ...straightforward workflow for doing gene targeting, reporter generation, controllable gene knockouts, and inducible gene...Strategy for leak-free transcriptional control. C) Iterative transduction workflow overview. Image from Sun...successfully targeted cells that can be used to generate organoid cultures with a modification of interest...

  17. Addgene's a Nonprofit? Nonprofit Awareness Day 2017

    Type
    Blog Post
    Published
    June 5, 2017, 3:19 p.m.
    ...that Addgene is officially filed, recognized and operated as a nonprofit under the United States Internal...fulfilling our mission. “Addgene’s nonprofit mission: Accelerate research and discovery by improving access to...in that we are able to fulfill our mission by generating revenue through distribution of research materials...nonprofit" is a bit misleading. Nonprofits can generate revenue (as Addgene does through materials distribution...nonprofit, open science and Addgene in our mission to accelerate research with easier sharing and collaboration...

  18. Antibodies 101: Fc Effector Functions

    Type
    Blog Post
    Published
    May 18, 2023, 1:15 p.m.
    ...besides structure and isotype determination, don’t generally affect antibody applications like ELISAs or Western...the receptors are named after the isotypes they interact with, so IgG isotypes bind to Fcγ receptors; IgE..., ADCC can be induced through a very similar interaction between IgE antibodies and eosinophils, where... induce?  Fc receptors are intriguing from a therapeutic standpoint. Engineered monoclonal antibodies ...phagocytosis for effective macrophage-based cancer immunotherapy.Sci. Adv.8,eabl9171(2022).DOI:10.1126/sciadv.abl9171...

  19. Oh, The Places You Can Go: Careers in Science Communication - Curriculum Development

    Type
    Blog Post
    Published
    Nov. 1, 2017, 2:04 p.m.
    ...that in January. This involves going in front of a camera and kind of walking them through the steps of, ... heart to look at the inside. We do a lot of interactives and videos, as well, to supplement the hands-on... Caitlin also collaborates with the sales and operations departments at eScience Labs. This can involve...eScience warehouse is onsite, Caitlin can visit the operations team to make sure “that everything that’s going...facilitator between her team, the sales department, and operations department with the ultimate goal of determining...

  20. Understanding GPCR Signaling Using TRUPATH

    Type
    Blog Post
    Published
    Dec. 14, 2020, 2:15 p.m.
    ...by constructing 14 luciferase donor Rluc8-Gα chimeras, four acceptor GFP2-Gγ chimeras, and two untagged... exhaustive refinement, it was not possible to generate an optimal Rluc8-Gα construct for two of the 16...treated with the luminescent enzyme substrate coelenterazine, followed by the addition of the endogenous...
Showing: 381 - 400 of 830 results